The Experts below are selected from a list of 894 Experts worldwide ranked by ideXlab platform
Wolfgang Blenau - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization and localization of the first tyrAmine Receptor of the American cockroach (Periplaneta americana)
Neuroscience, 2009Co-Authors: Carmen Rotte, C. Krach, Sabine Balfanz, Arnd Baumann, B. Walz, Wolfgang BlenauAbstract:The phenolAmines octopAmine and tyrAmine control, regulate, and modulate many physiological and behavioral processes in invertebrates. Vertebrates possess only small amounts of both substances, and thus, octopAmine and tyrAmine, together with other Biogenic Amines, are referred to as "trace Amines." Biogenic Amines evoke cellular responses by activating G-protein-coupled Receptors. We have isolated a complementary DNA (cDNA) that encodes a Biogenic Amine Receptor from the American cockroach Periplaneta americana, viz., Peatyr1, which shares high sequence similarity to members of the invertebrate tyrAmine-Receptor family. The PeaTYR1 Receptor was stably expressed in human embryonic kidney (HEK) 293 cells, and its ligand response has been exAmined. Receptor activation with tyrAmine reduces adenylyl cyclase activity in a dose-dependent manner (EC50 ∼350 nM). The inhibitory effect of tyrAmine is abolished by co-incubation with either yohimbine or chlorpromazine. Receptor expression has been investigated by reverse transcription polymerase chain reaction and immunocytochemistry. The mRNA is present in various tissues including brain, salivary glands, midgut, Malpighian tubules, and leg muscles. The effect of tyrAmine on salivary gland acinar cells has been investigated by intracellular recordings, which have revealed excitatory presynaptic actions of tyrAmine. This study marks the first comprehensive molecular, pharmacological, and functional characterization of a tyrAmine Receptor in the cockroach. © 2009 IBRO.
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a review of neurohormone gpcrs present in the fruitfly drosophila melanogaster and the honey bee apis mellifera
Progress in Neurobiology, 2006Co-Authors: Frank Hauser, Wolfgang Blenau, Giuseppe Cazzamali, Michael Williamson, Cornelis J P GrimmelikhuijzenAbstract:G protein-coupled Receptor (GPCR) genes are large gene families in every animal, sometimes making up to 1-2% of the animal's genome. Of all insect GPCRs, the neurohormone (neuropeptide, protein hormone, Biogenic Amine) GPCRs are especially important, because they, together with their ligands, occupy a high hierarchic position in the physiology of insects and steer crucial processes such as development, reproduction, and behavior. In this paper, we give a review of our current knowledge on Drosophila melanogaster GPCRs and use this information to annotate the neurohormone GPCR genes present in the recently sequenced genome from the honey bee Apis mellifera. We found 35 neuropeptide Receptor genes in the honey bee (44 in Drosophila) and two genes, coding for leucine-rich repeats-containing protein hormone GPCRs (4 in Drosophila). In addition, the honey bee has 19 Biogenic Amine Receptor genes (21 in Drosophila). The larger numbers of neurohormone Receptors in Drosophila are probably due to gene duplications that occurred during recent evolution of the fly. Our analyses also yielded the likely ligands for 40 of the 56 honey bee neurohormone GPCRs identified in this study. In addition, we made some interesting observations on neurohormone GPCR evolution and the evolution and co-evolution of their ligands. For neuropeptide and protein hormone GPCRs, there appears to be a general co-evolution between Receptors and their ligands. This is in contrast to Biogenic Amine GPCRs, where evolutionarily unrelated GPCRs often bind to the same Biogenic Amine, suggesting frequent ligand exchanges ("ligand hops") during GPCR evolution.
Benjamin P Oldroyd - One of the best experts on this subject based on the ideXlab platform.
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Effects of natural mating and CO2 narcosis on Biogenic Amine Receptor gene expression in the ovaries and brain of queen honey bees, Apis mellifera.
Insect Molecular Biology, 2012Co-Authors: Vanina Vergoz, Michael Duncan, Julianne Lim, Guénaël Cabanes, Benjamin P OldroydAbstract:A queen honey bee mates at ~6 days of age, storing the sperm in her spermatheca for life. Mating is associated with profound changes in the behaviour and physiology of the queen but the mechanisms underlying these changes are poorly understood. What is known is that the presence of semen in the oviducts and spermatheca is insufficient to initiate laying, and that copulation or CO2 narcosis is necessary for ovary activation. In this study we use real-time quantitative PCR to investigate the expression of Biogenic Amine Receptor genes in the brain and ovarian tissue of queens in relation to their reproductive status. We show that dopAmine, octopAmine and serotonin Receptor genes are expressed in the ovaries of queens, and that natural mating, CO2 narcosis, and the presence of semen in the spermatheca differentially affect their expression. We suggest that these changes may be central to the hormonal cascades that are necessary to initiate oogenesis.
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Biogenic Amine Receptor gene expression in the ovarian tissue of the honey bee apis mellifera
Insect Molecular Biology, 2012Co-Authors: Vanina Vergoz, Julianne Lim, Benjamin P OldroydAbstract:In the honey bee Apis mellifera loss of the queen from a colony induces increased levels of the Biogenic Amine dopAmine in the brain of workers, and this elevation is correlated with ovary activation. In the present study we use real-time quantitative PCR to investigate expression of five Biogenic Amine Receptor genes. We show that Biogenic Amine Receptors are expressed in ovarian tissue, and that their expression is strongly influenced by the presence or absence of a queen in the colony. In contrast to the brain, where all three dopAmine Receptors are expressed, only two dopAmine Receptors are expressed in the ovaries, and their expression is strongly correlated with the reproductive status of workers. We conclude that Biogenic Amine Receptors are expressed in the ovaries and are likely to be directly influential in the regulation of worker sterility in honey bees.
Päivi H. Torkkeli - One of the best experts on this subject based on the ideXlab platform.
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Multiple Biogenic Amine Receptor Types Modulate Spider, Cupiennius salei, Mechanosensory Neurons
Frontiers Media S.A., 2018Co-Authors: Vaishnavi Sukumar, Hongxia Liu, Shannon Meisner, Andrew S. French, Päivi H. TorkkeliAbstract:The Biogenic Amines octopAmine (OA), tyrAmine (TA), dopAmine (DA), serotonin (5-HT), and histAmine (HA) affect diverse physiological and behavioral processes in invertebrates, but recent findings indicate that an additional adrenergic system exists in at least some invertebrates. Transcriptome analysis has made it possible to identify Biogenic Amine Receptor genes in a wide variety of species whose genomes have not yet been sequenced. This approach provides new sequences for research into the evolutionary history of Biogenic Amine Receptors and allows them to be studied in experimentally accessible animal models. The Central American Wandering spider, Cupiennius salei, is an experimental model for neurophysiological, developmental and behavioral research. We identified ten different Biogenic Amine Receptors in C. salei transcriptomes. Phylogenetic analysis indicated that, in addition to the typical Receptors for OA, TA, DA, and 5-HT in protostome invertebrates, spiders also have α1- and α2-adrenergic Receptors, but lack TAR2 Receptors and one invertebrate specific DA Receptor type. In situ hybridization revealed four types of Biogenic Amine Receptors expressed in C. salei mechanosensory neurons. We used intracellular electrophysiological experiments and pharmacological tools to determine how each Receptor type contributes to modulation of these neurons. We show that arachnids have similar groups of Biogenic Amine Receptors to other protostome invertebrates, but they lack two clades. We also clarify that arachnids and many other invertebrates have both α1- and α2-adrenergic, likely OA Receptors. Our results indicate that in addition to an OAβ-Receptor that regulates rapid and large changes in sensitivity via a Gs-protein activating a cAMP mediated pathway, the C. salei mechanosensory neurons have a constitutively active TAR1 and/or α2-adrenergic Receptor type that adjusts the baseline sensitivity to a level appropriate for the behavioral state of the animal by a Gq-protein that mobilizes Ca2+
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Table_1_Multiple Biogenic Amine Receptor Types Modulate Spider, Cupiennius salei, Mechanosensory Neurons.PDF
2018Co-Authors: Vaishnavi Sukumar, Hongxia Liu, Shannon Meisner, Andrew S. French, Päivi H. TorkkeliAbstract:The Biogenic Amines octopAmine (OA), tyrAmine (TA), dopAmine (DA), serotonin (5-HT), and histAmine (HA) affect diverse physiological and behavioral processes in invertebrates, but recent findings indicate that an additional adrenergic system exists in at least some invertebrates. Transcriptome analysis has made it possible to identify Biogenic Amine Receptor genes in a wide variety of species whose genomes have not yet been sequenced. This approach provides new sequences for research into the evolutionary history of Biogenic Amine Receptors and allows them to be studied in experimentally accessible animal models. The Central American Wandering spider, Cupiennius salei, is an experimental model for neurophysiological, developmental and behavioral research. We identified ten different Biogenic Amine Receptors in C. salei transcriptomes. Phylogenetic analysis indicated that, in addition to the typical Receptors for OA, TA, DA, and 5-HT in protostome invertebrates, spiders also have α1- and α2-adrenergic Receptors, but lack TAR2 Receptors and one invertebrate specific DA Receptor type. In situ hybridization revealed four types of Biogenic Amine Receptors expressed in C. salei mechanosensory neurons. We used intracellular electrophysiological experiments and pharmacological tools to determine how each Receptor type contributes to modulation of these neurons. We show that arachnids have similar groups of Biogenic Amine Receptors to other protostome invertebrates, but they lack two clades. We also clarify that arachnids and many other invertebrates have both α1- and α2-adrenergic, likely OA Receptors. Our results indicate that in addition to an OAβ-Receptor that regulates rapid and large changes in sensitivity via a Gs-protein activating a cAMP mediated pathway, the C. salei mechanosensory neurons have a constitutively active TAR1 and/or α2-adrenergic Receptor type that adjusts the baseline sensitivity to a level appropriate for the behavioral state of the animal by a Gq-protein that mobilizes Ca2+.
Vanina Vergoz - One of the best experts on this subject based on the ideXlab platform.
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Effects of natural mating and CO2 narcosis on Biogenic Amine Receptor gene expression in the ovaries and brain of queen honey bees, Apis mellifera.
Insect Molecular Biology, 2012Co-Authors: Vanina Vergoz, Michael Duncan, Julianne Lim, Guénaël Cabanes, Benjamin P OldroydAbstract:A queen honey bee mates at ~6 days of age, storing the sperm in her spermatheca for life. Mating is associated with profound changes in the behaviour and physiology of the queen but the mechanisms underlying these changes are poorly understood. What is known is that the presence of semen in the oviducts and spermatheca is insufficient to initiate laying, and that copulation or CO2 narcosis is necessary for ovary activation. In this study we use real-time quantitative PCR to investigate the expression of Biogenic Amine Receptor genes in the brain and ovarian tissue of queens in relation to their reproductive status. We show that dopAmine, octopAmine and serotonin Receptor genes are expressed in the ovaries of queens, and that natural mating, CO2 narcosis, and the presence of semen in the spermatheca differentially affect their expression. We suggest that these changes may be central to the hormonal cascades that are necessary to initiate oogenesis.
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Biogenic Amine Receptor gene expression in the ovarian tissue of the honey bee apis mellifera
Insect Molecular Biology, 2012Co-Authors: Vanina Vergoz, Julianne Lim, Benjamin P OldroydAbstract:In the honey bee Apis mellifera loss of the queen from a colony induces increased levels of the Biogenic Amine dopAmine in the brain of workers, and this elevation is correlated with ovary activation. In the present study we use real-time quantitative PCR to investigate expression of five Biogenic Amine Receptor genes. We show that Biogenic Amine Receptors are expressed in ovarian tissue, and that their expression is strongly influenced by the presence or absence of a queen in the colony. In contrast to the brain, where all three dopAmine Receptors are expressed, only two dopAmine Receptors are expressed in the ovaries, and their expression is strongly correlated with the reproductive status of workers. We conclude that Biogenic Amine Receptors are expressed in the ovaries and are likely to be directly influential in the regulation of worker sterility in honey bees.
Carmen Rotte - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization and localization of the first tyrAmine Receptor of the American cockroach (Periplaneta americana)
Neuroscience, 2009Co-Authors: Carmen Rotte, C. Krach, Sabine Balfanz, Arnd Baumann, B. Walz, Wolfgang BlenauAbstract:The phenolAmines octopAmine and tyrAmine control, regulate, and modulate many physiological and behavioral processes in invertebrates. Vertebrates possess only small amounts of both substances, and thus, octopAmine and tyrAmine, together with other Biogenic Amines, are referred to as "trace Amines." Biogenic Amines evoke cellular responses by activating G-protein-coupled Receptors. We have isolated a complementary DNA (cDNA) that encodes a Biogenic Amine Receptor from the American cockroach Periplaneta americana, viz., Peatyr1, which shares high sequence similarity to members of the invertebrate tyrAmine-Receptor family. The PeaTYR1 Receptor was stably expressed in human embryonic kidney (HEK) 293 cells, and its ligand response has been exAmined. Receptor activation with tyrAmine reduces adenylyl cyclase activity in a dose-dependent manner (EC50 ∼350 nM). The inhibitory effect of tyrAmine is abolished by co-incubation with either yohimbine or chlorpromazine. Receptor expression has been investigated by reverse transcription polymerase chain reaction and immunocytochemistry. The mRNA is present in various tissues including brain, salivary glands, midgut, Malpighian tubules, and leg muscles. The effect of tyrAmine on salivary gland acinar cells has been investigated by intracellular recordings, which have revealed excitatory presynaptic actions of tyrAmine. This study marks the first comprehensive molecular, pharmacological, and functional characterization of a tyrAmine Receptor in the cockroach. © 2009 IBRO.