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Nabil Zouari - One of the best experts on this subject based on the ideXlab platform.
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Application of statistical experimental design for optimisation of Bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium
Brazilian Journal of Microbiology, 2013Co-Authors: Saoussen Ben Khedher, Samir Jaoua, Nabil ZouariAbstract:In order to overproduce Bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on Bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L -1 starch, 30 g L -1 soya bean an d9gL -1 sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis Bioinsecticides at industrial scale which is of great importance in practical point of view.
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Improvement of Photorhabdus temperata strain K122 Bioinsecticide production by batch and fed-batch fermentations optimization
Bioprocess and Biosystems Engineering, 2012Co-Authors: Wafa Jallouli, Samir Jaoua, Nabil ZouariAbstract:Optimization of a fermentation process for Bioinsecticides production by Photorhabdus temperata strain K122 was investigated into fully controlled 3-L fermenter using an optimized medium (OM). Development of large-scale inocula showed that the composition of the growth medium greatly influenced the physiological state of P . temperata cells. The effect of pH, agitation and dissolved oxygen concentration (DO) on the growth, culturability and oral toxicity of P . temperata cells were also investigated. Indeed, maintaining the pH at 7 and controlling DO concentration at 50 % saturation throughout the fermentation process, improved biomass production, CFU counts and oral toxicity by 41.1, 35 and 32.1 %, respectively, as compared to cultures carried out in 500 mL shake flasks. At such conditions, 8 g/L glucose fed-batch fermentation, enhanced cell lysis and variants small colony (Vsm) polymorphism appearance. To overcome such limitations, glucose concentration should be maintained at 4 g/L. In this case, P . temperata cells were produced at high cell density and culturability reaching 4.5 and 1.2 × 10^9 cells/mL, respectively. In addition, the stability of the primary form was maintained for a long period in the stationary growth phase and Vsm polymorphism was completely avoided that can be crucial for scale-up the bioprocess of P. temperata Bioinsecticide.
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Improvement of Photorhabdus temperata Bioinsecticides production in low‐cost media through adequate fermentation technology
Biotechnology Progress, 2012Co-Authors: Wafa Jallouli, Samir Jaoua, Nabil ZouariAbstract:To develop a cost effective process for Bioinsecticides production by Photorhabdus temperata, dissolved oxygen (DO) requirements were investigated in both the complex and the optimized media using diluted seawater as a source of micronutrients. By varying DO concentrations, tolerance to hydrogen peroxide was shown to be medium dependant. Indeed, P. temperata cells grown in the complex medium, exhibited higher tolerance than cells grown in the optimized medium (OM). Tolerance to H2O2 was shown to be related to intracellular reactive oxygen species (ROS) accumulation during soya bean meal or glucose assimilation, as shown by flow cytometry analysis. To avoid oxidative stress damages in P. temperata cells cultured in the OM, DO concentration should be constant 50% saturation throughout the fermentation. However, a DO-shift control strategy was demonstrated to be beneficial for P. temperata Bioinsecticide production in the complex medium. By using such a strategy biomass, culturability, and oral toxicity reached 16.5 × 108, 1.15 × 108 cells/mL and 64.2%, respectively, thus was 16.19, 26.37, and 12.2% more than in the cultures carried out at a constant 50% saturation. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
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Overcoming the production limitations of Photorhabdus temperata ssp. temperata strain K122 Bioinsecticides in low-cost medium
Bioprocess and Biosystems Engineering, 2011Co-Authors: Wafa Jallouli, Samir Jaoua, Nabil ZouariAbstract:For low-cost production of Photorhabdus temperata ssp. temperata strain K122 Bioinsecticide, a cheap complex medium was optimized. Diluted seawater was used as the source of micronutrients, especially sodium chloride, involved in the improvement of cell density, culturability and oral toxicity of the bacterium P . temperata against Ephestia kuehniella larvae. Thus, the new formulated medium was composed only of 10 g/l of soya bean meal, used as the carbon and nitrogen main source, mixed in sevenfold diluted seawater. At such conditions, several limitations of P . temperata Bioinsecticide productions were shown to be overcome. The appearance of variants small colony polymorphism was completely avoided. Thus, the strain K122 was maintained at the primary form even after prolonged incubation. Moreover, the viable but nonculturable state was partially overcome, since the ability of P . temperata cells to form colonies on the solid medium was prolonged until 78 h of incubation. In addition, when cultured in the complex medium, P . temperata cells were produced at high cell density of 12 × 10^8 cells/ml and exhibited 81.48% improvement of oral toxicity compared to those produced in the optimized medium. With such medium, the large-scale Bioinsecticides production into 3-l fully controlled fermenter improved the total cell counts, CFU counts and oral toxicity by 20, 5.81 and 16.73%, respectively. This should contribute to a significant reduction of production cost of highly potent P . temperata strain K122 cells, useful as a Bioinsecticide.
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Improvement of Bioinsecticides production by sporeless Bacillus thuringiensis strains in response to various stresses in low cost medium.
Current Microbiology, 2011Co-Authors: Saoussen Ben Khedher, Samir Jaoua, Nabil ZouariAbstract:The use of Bioinsecticides, particularly those produced by sporeless Bacillus thuringiensis strains, has been shown to be a good alternative in pest management. Two types of sporeless mutants were distinguished. The asporogenic mutants which completely lack spores produce a regular bipyramidal crystal inclusion. The oligosporogenic mutants kept the ability to produce insecticidal crystal proteins. However, sporulation in such mutants was not totally blocked and very few of them could still produce spores. In order to improve Bioinsecticides production, adaptation of sporeless strains to heat shock and osmotic stress was investigated. Delta-endotoxin production by 78% of sporeless mutants was significantly improved by osmotic stress with an overproduction of about 17%, compared to the wild strain BNS3. However, toxin production was improved by only 21% of mutants after heat shock, in low cost medium. The statistical analysis proved that delta-endotoxin production, cell growth, and spore formation of asporogenic and oligosporogenic mutants depended on the type of applied stress. Each strain has an important potential when applying the adequate stress. Moreover, adaptation of sporeless mutants to NaCl may allow the substitution of all minerals of the medium by diluted sea water which appeared to be a good alternative for the economic production of Bioinsecticides at industrial scale which is of great importance from the practical point of view.
Siti Herlinda - One of the best experts on this subject based on the ideXlab platform.
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Herbivore insects and predatory arthropods in freshwater swamp rice field in South Sumatra, Indonesia sprayed with Bioinsecticides of entomopathogenic fungi and abamectin
Biodiversitas Journal of Biological Diversity, 2020Co-Authors: Siti Herlinda, Tili Karenina, Yulia Pujiastuti, Ghanni Prabawati, Susilawati Susilawati, Hasbi Hasbi, Chandra IrsanAbstract:Abstract. Herlinda S, Prabawati G, Pujiastuti Y, Susilawati, Karenia T, Hasbi, Irsan C. 2020. Herbivore insects and predatory arthropods in freshwater swamp rice field in South Sumatra, Indonesia sprayed with Bioinsecticides of entomopathogenic fungi and abamectin. Biodiversitas 21: 3755-3768. Herbivore insect population and predatory arthropods in rice field may be effected by the application entomopathogenic fungi or synthetic insecticide. The objective of this research was to analyze individual quantity of herbivore insects and predatory arthropod inhabiting freshwater swamp rice fields treated with Bioinsecticides and abamectin (commercial insecticide). This research was conducted in the freshwater swamp rice field located in Village Pelabuhan Dalam, Sub District Pemulutan, District Ogan Ilir, and South Sumatra. The experiment was arranged in a Completely Randomized Block Design consisted of four plots of treatment. The research used the Bioinsecticides made from entomopathogenic fungi (Beauveria bassiana, Metarhizium anisopliae, and Cordyceps military), and abamectin. Sampling to collect arthropods inhabiting rice canopy was also conducted using an entomological net in the study locations. This research found 12 families of herbivore insects with 22 species dominated by Nilaparvata lugens and Leptocorisa acuta and 32 species of spider belonged to eight families dominated by Tetragnatha virescens and Oxyopes matiensis. The species diversity of spider was higher in the plots of the Bioinsecticide compared to that of the abamectin. Predatory insects found belonged to 14 species belonged to eight families dominated by species of Ophionea nigrofasciata, Verania discolor, and Paedorus fuscipe. The abundance of predatory insects in plots sprayed with the Bioinsecticides was higher compared to that of the abamectin plot. The abundance and species diversity of predatory arthropod sprayed with Bioinsecticide of B. bassiana, M. anisopliae, and C. militaris did not decrease, while the population of herbivore insect tended to decrease.
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Efikasi Bioinsektisida Bacillus thuringiensis Barliner terhadap Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) pada Tanaman Padi Utama dan Ratun
2020Co-Authors: Khoirul Ikhsanudin Hanif, Siti Herlinda, Suwandi Suwandi, Tili KareninaAbstract:Hanif et al, 2017. Efficacy of Bacillus thuringiensis Barliner Bioinsecticide against Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) in Major Rice Crops and Ratooning Rice. JLSO 6(1):95-105. Crickets ( Gryllus bimaculatus ) are an important pests on rice that can reduce productivity. The application of Bacillus thuringiensis Bioinsecticide is expected to be an alternative methode to control these pest. The objective of the study was to investigate optimal dose of B. thuringiensis in suppressing rice damage was caused by G. bimaculatus . The observation were conducted on rice cultivation in Pelabuhan Dalam Village, Pemulutan Subdistrict of Ogan Ilir Regency. The results showed that application of B. thuringiensis Bioinsecticide had significant effects in suppressing rice crop damage by G. bimaculatus , but did not had significant effect on growth of rice plants.
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Efikasi Bioinsektisida Beauveria bassiana (Bals.) Vuill Terhadap Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) Pada Padi Ratun
2020Co-Authors: Soleha Soleha, Siti Herlinda, Suparman SuparmanAbstract:Soleha, et al. Efficacy of Bioinsecticide from Beauveria bassiana (Bals.) Vuill on Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) on Ratooning Rice in Fresh Swamps. JLSO 5(2):189-197. The use of chemical insecticide to control pest of crop often causes the bad impact on nontarget organisms. B. bassiana is an entomopathogenic fungi has depeloved to control pest of crop as chemical insecticide alternative. This study aimed to measure mortality and damages rate by crickets to rice of the applications of Bioinsecticide containing B. bassiana. The Method of this study used Random Block Designs with 5 treatments and 4 replications. The treatments applications were 1 L.ha -1 , 2 L.ha -1 , 3 L.ha -1 , commercial B. bassiana as recommended, and 0 L.ha -1 as control. The applications Bioinsecticide containing B. bassiana was significant to nymph of crickets mortality. Mortality of nymph of the applications of B. bassiana was significant with nontreatment (0 L.ha-1). The highest mortality was found in the Bb 3 L .ha-1 about 52,50% and the lowest mortality on 0 L.ha -1 . The shortest LT50 was occurred in Bb 3 L.ha -1 about 7,00 days, while the longest LT50 occurred in Bb 0 L.ha -1 about 50,47 – 64,80 days. The highest damages was found in Bb 0 L.ha -1 about 11,55% - 34,14% and the lowest damages was found in Bb 3 L.ha -1 about 5,94 % - 19,82%. Application Bioinsecticide containing B. bassiana is significant to crickets mortality and will make an important contribution to the biological control for insect pests.
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The impact of Bioinsecticide overdoses of Beauveria bassiana on species diversity and abundance of not targeted arthropods in South Sumatra (Indonesia) freshwater swamp paddy
Biodiversitas, 2020Co-Authors: Khoirul Ikhsanudin Hanif, Siti Herlinda, Chandra Irsan, Yulia Pujiastuti, Ghanni Prabawati, Hasbi, Tili KareninaAbstract:Abstract. Hanif KI, Herlinda S, Irsan C, Pujiastuti Y, Prabawati G, Hasbi, Karenina T. 2020. The impact of Bioinsecticide overdoses of Beauveria bassiana on species diversity and abundance of not targeted arthropods in South Sumatra (Indonesia) freshwater swamp paddy. Biodiversitas 21: 2124-2136. Bioinsecticides have been shown to reduce insect pest populations, but it is necessary to monitor the effect of Bioinsecticide overdoses on non-target arthropods because they are abundant in rice fields. This study aimed to analyze the impact of Bioinsecticide overdoses of B. bassiana on species diversity and abundance of non-target arthropods. Bioinsecticides derived from Beauveria bassiana were sprayed on the paddy every two weeks until the ripening stage, at a dose of 1, 2, 3, 4 L.ha-1, control (without insecticide), and abamectin as prescribed. The abundance of spiders and predatory arthropods did not decrease when Bioinsecticides were applied but the abundance of spiders, especially Lycosidae and Salticidae dropped significantly when abamectin was applied. The abundance of the parasitoid decreased significantly when abamectin was applied, but the abundance of parasitoids applied by Bioinsecticide was not significantly different from those of control. The abundance of insect pests started to decrease when Bioinsecticide was applied to 2, 3 or 4 L.ha-1. The diversity species of non-target arthropods (spiders, predatory arthropods, and parasitoids) tended to decrease when it was applied Bioinsecticides 3 and 4 L.ha-1 or abamectin, but their abundance remained high and no species dominance was found. For this reason, the correct dosage for suppressing insect pests abundance by not decreasing the abundance and diversity of non-target species is 2 L.ha-1 and applied when herbivore abundance is high.
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The abundance of canopy arthropods in South Sumatra (Indonesia) freshwater swamp main and ratooned rice applied with Bioinsecticides and synthetic insecticide
Biodiversitas, 2019Co-Authors: Ghanni Prabawati, Siti Herlinda, Yulia PujiastutiAbstract:Abstract. Prabawati G, Herlinda S, Pujiastuti Y. 2019. A comparative study on the arboreal arthropod abundance of rice fields between applied with fungal Bioinsecticides and abamectin in the freshwater swamp of South Sumatra, Indonesia (Study case in the main and ratoon rice fields). Biodiversitas 20: 2921-2930. Ratoon rice has been cultivated by local farmers in South Sumatra and can be a source of habitat and niches for the main rice arthropods. The study aimed to compare the abundance of arthropods between main and rationed rice field applied with fungal Bioinsecticides and abamectin. This study used Bioinsecticides from fungi of Beauveria bassiana, Metarhizium anisopliae, and Cordyceps militaris and control using abamectin. Arboreal arthropods sampled using a sweep net. The abundance of arthropods in the main rice tends to be higher than in ratoon. Spiders found during the two rice seasons were Araneidae, Linyphiidae, Lycosidae, Oxyopidae, Salticidae, Theridiidae, Tetragnathidae, Theridiosomatidae, while predatory insects found were Mantidae, Coenagrionidae, Staphylinidae, Anthicidae, Latridiidae, Formicidae, Coccinellidae, Coccinellidae, Tettigoniidae, Miridae, Carabidae. The fungal Bioinsecticides did not reduce the predatory arthropod abundance but the abamectin caused a decrease in the abundance of all guilds. The most drastic decrease was found in web-building spiders. The movement of hunting spiders and the predatory insects of the main to ratoon rice was faster than that of web-building spiders. The existence of ratoon rice could become habitats and niches for arthropods from the main rice. For this reason, controlling the herbivore insects could use Bioinsecticides derived from entomopathogenic fungi.
Tili Karenina - One of the best experts on this subject based on the ideXlab platform.
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Herbivore insects and predatory arthropods in freshwater swamp rice field in South Sumatra, Indonesia sprayed with Bioinsecticides of entomopathogenic fungi and abamectin
Biodiversitas Journal of Biological Diversity, 2020Co-Authors: Siti Herlinda, Tili Karenina, Yulia Pujiastuti, Ghanni Prabawati, Susilawati Susilawati, Hasbi Hasbi, Chandra IrsanAbstract:Abstract. Herlinda S, Prabawati G, Pujiastuti Y, Susilawati, Karenia T, Hasbi, Irsan C. 2020. Herbivore insects and predatory arthropods in freshwater swamp rice field in South Sumatra, Indonesia sprayed with Bioinsecticides of entomopathogenic fungi and abamectin. Biodiversitas 21: 3755-3768. Herbivore insect population and predatory arthropods in rice field may be effected by the application entomopathogenic fungi or synthetic insecticide. The objective of this research was to analyze individual quantity of herbivore insects and predatory arthropod inhabiting freshwater swamp rice fields treated with Bioinsecticides and abamectin (commercial insecticide). This research was conducted in the freshwater swamp rice field located in Village Pelabuhan Dalam, Sub District Pemulutan, District Ogan Ilir, and South Sumatra. The experiment was arranged in a Completely Randomized Block Design consisted of four plots of treatment. The research used the Bioinsecticides made from entomopathogenic fungi (Beauveria bassiana, Metarhizium anisopliae, and Cordyceps military), and abamectin. Sampling to collect arthropods inhabiting rice canopy was also conducted using an entomological net in the study locations. This research found 12 families of herbivore insects with 22 species dominated by Nilaparvata lugens and Leptocorisa acuta and 32 species of spider belonged to eight families dominated by Tetragnatha virescens and Oxyopes matiensis. The species diversity of spider was higher in the plots of the Bioinsecticide compared to that of the abamectin. Predatory insects found belonged to 14 species belonged to eight families dominated by species of Ophionea nigrofasciata, Verania discolor, and Paedorus fuscipe. The abundance of predatory insects in plots sprayed with the Bioinsecticides was higher compared to that of the abamectin plot. The abundance and species diversity of predatory arthropod sprayed with Bioinsecticide of B. bassiana, M. anisopliae, and C. militaris did not decrease, while the population of herbivore insect tended to decrease.
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Efikasi Bioinsektisida Bacillus thuringiensis Barliner terhadap Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) pada Tanaman Padi Utama dan Ratun
2020Co-Authors: Khoirul Ikhsanudin Hanif, Siti Herlinda, Suwandi Suwandi, Tili KareninaAbstract:Hanif et al, 2017. Efficacy of Bacillus thuringiensis Barliner Bioinsecticide against Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) in Major Rice Crops and Ratooning Rice. JLSO 6(1):95-105. Crickets ( Gryllus bimaculatus ) are an important pests on rice that can reduce productivity. The application of Bacillus thuringiensis Bioinsecticide is expected to be an alternative methode to control these pest. The objective of the study was to investigate optimal dose of B. thuringiensis in suppressing rice damage was caused by G. bimaculatus . The observation were conducted on rice cultivation in Pelabuhan Dalam Village, Pemulutan Subdistrict of Ogan Ilir Regency. The results showed that application of B. thuringiensis Bioinsecticide had significant effects in suppressing rice crop damage by G. bimaculatus , but did not had significant effect on growth of rice plants.
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The impact of Bioinsecticide overdoses of Beauveria bassiana on species diversity and abundance of not targeted arthropods in South Sumatra (Indonesia) freshwater swamp paddy
Biodiversitas, 2020Co-Authors: Khoirul Ikhsanudin Hanif, Siti Herlinda, Chandra Irsan, Yulia Pujiastuti, Ghanni Prabawati, Hasbi, Tili KareninaAbstract:Abstract. Hanif KI, Herlinda S, Irsan C, Pujiastuti Y, Prabawati G, Hasbi, Karenina T. 2020. The impact of Bioinsecticide overdoses of Beauveria bassiana on species diversity and abundance of not targeted arthropods in South Sumatra (Indonesia) freshwater swamp paddy. Biodiversitas 21: 2124-2136. Bioinsecticides have been shown to reduce insect pest populations, but it is necessary to monitor the effect of Bioinsecticide overdoses on non-target arthropods because they are abundant in rice fields. This study aimed to analyze the impact of Bioinsecticide overdoses of B. bassiana on species diversity and abundance of non-target arthropods. Bioinsecticides derived from Beauveria bassiana were sprayed on the paddy every two weeks until the ripening stage, at a dose of 1, 2, 3, 4 L.ha-1, control (without insecticide), and abamectin as prescribed. The abundance of spiders and predatory arthropods did not decrease when Bioinsecticides were applied but the abundance of spiders, especially Lycosidae and Salticidae dropped significantly when abamectin was applied. The abundance of the parasitoid decreased significantly when abamectin was applied, but the abundance of parasitoids applied by Bioinsecticide was not significantly different from those of control. The abundance of insect pests started to decrease when Bioinsecticide was applied to 2, 3 or 4 L.ha-1. The diversity species of non-target arthropods (spiders, predatory arthropods, and parasitoids) tended to decrease when it was applied Bioinsecticides 3 and 4 L.ha-1 or abamectin, but their abundance remained high and no species dominance was found. For this reason, the correct dosage for suppressing insect pests abundance by not decreasing the abundance and diversity of non-target species is 2 L.ha-1 and applied when herbivore abundance is high.
Samir Jaoua - One of the best experts on this subject based on the ideXlab platform.
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Application of statistical experimental design for optimisation of Bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium
Brazilian Journal of Microbiology, 2013Co-Authors: Saoussen Ben Khedher, Samir Jaoua, Nabil ZouariAbstract:In order to overproduce Bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on Bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L -1 starch, 30 g L -1 soya bean an d9gL -1 sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis Bioinsecticides at industrial scale which is of great importance in practical point of view.
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Improvement of Photorhabdus temperata strain K122 Bioinsecticide production by batch and fed-batch fermentations optimization
Bioprocess and Biosystems Engineering, 2012Co-Authors: Wafa Jallouli, Samir Jaoua, Nabil ZouariAbstract:Optimization of a fermentation process for Bioinsecticides production by Photorhabdus temperata strain K122 was investigated into fully controlled 3-L fermenter using an optimized medium (OM). Development of large-scale inocula showed that the composition of the growth medium greatly influenced the physiological state of P . temperata cells. The effect of pH, agitation and dissolved oxygen concentration (DO) on the growth, culturability and oral toxicity of P . temperata cells were also investigated. Indeed, maintaining the pH at 7 and controlling DO concentration at 50 % saturation throughout the fermentation process, improved biomass production, CFU counts and oral toxicity by 41.1, 35 and 32.1 %, respectively, as compared to cultures carried out in 500 mL shake flasks. At such conditions, 8 g/L glucose fed-batch fermentation, enhanced cell lysis and variants small colony (Vsm) polymorphism appearance. To overcome such limitations, glucose concentration should be maintained at 4 g/L. In this case, P . temperata cells were produced at high cell density and culturability reaching 4.5 and 1.2 × 10^9 cells/mL, respectively. In addition, the stability of the primary form was maintained for a long period in the stationary growth phase and Vsm polymorphism was completely avoided that can be crucial for scale-up the bioprocess of P. temperata Bioinsecticide.
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Improvement of Photorhabdus temperata Bioinsecticides production in low‐cost media through adequate fermentation technology
Biotechnology Progress, 2012Co-Authors: Wafa Jallouli, Samir Jaoua, Nabil ZouariAbstract:To develop a cost effective process for Bioinsecticides production by Photorhabdus temperata, dissolved oxygen (DO) requirements were investigated in both the complex and the optimized media using diluted seawater as a source of micronutrients. By varying DO concentrations, tolerance to hydrogen peroxide was shown to be medium dependant. Indeed, P. temperata cells grown in the complex medium, exhibited higher tolerance than cells grown in the optimized medium (OM). Tolerance to H2O2 was shown to be related to intracellular reactive oxygen species (ROS) accumulation during soya bean meal or glucose assimilation, as shown by flow cytometry analysis. To avoid oxidative stress damages in P. temperata cells cultured in the OM, DO concentration should be constant 50% saturation throughout the fermentation. However, a DO-shift control strategy was demonstrated to be beneficial for P. temperata Bioinsecticide production in the complex medium. By using such a strategy biomass, culturability, and oral toxicity reached 16.5 × 108, 1.15 × 108 cells/mL and 64.2%, respectively, thus was 16.19, 26.37, and 12.2% more than in the cultures carried out at a constant 50% saturation. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
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Overcoming the production limitations of Photorhabdus temperata ssp. temperata strain K122 Bioinsecticides in low-cost medium
Bioprocess and Biosystems Engineering, 2011Co-Authors: Wafa Jallouli, Samir Jaoua, Nabil ZouariAbstract:For low-cost production of Photorhabdus temperata ssp. temperata strain K122 Bioinsecticide, a cheap complex medium was optimized. Diluted seawater was used as the source of micronutrients, especially sodium chloride, involved in the improvement of cell density, culturability and oral toxicity of the bacterium P . temperata against Ephestia kuehniella larvae. Thus, the new formulated medium was composed only of 10 g/l of soya bean meal, used as the carbon and nitrogen main source, mixed in sevenfold diluted seawater. At such conditions, several limitations of P . temperata Bioinsecticide productions were shown to be overcome. The appearance of variants small colony polymorphism was completely avoided. Thus, the strain K122 was maintained at the primary form even after prolonged incubation. Moreover, the viable but nonculturable state was partially overcome, since the ability of P . temperata cells to form colonies on the solid medium was prolonged until 78 h of incubation. In addition, when cultured in the complex medium, P . temperata cells were produced at high cell density of 12 × 10^8 cells/ml and exhibited 81.48% improvement of oral toxicity compared to those produced in the optimized medium. With such medium, the large-scale Bioinsecticides production into 3-l fully controlled fermenter improved the total cell counts, CFU counts and oral toxicity by 20, 5.81 and 16.73%, respectively. This should contribute to a significant reduction of production cost of highly potent P . temperata strain K122 cells, useful as a Bioinsecticide.
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Improvement of Bioinsecticides production by sporeless Bacillus thuringiensis strains in response to various stresses in low cost medium.
Current Microbiology, 2011Co-Authors: Saoussen Ben Khedher, Samir Jaoua, Nabil ZouariAbstract:The use of Bioinsecticides, particularly those produced by sporeless Bacillus thuringiensis strains, has been shown to be a good alternative in pest management. Two types of sporeless mutants were distinguished. The asporogenic mutants which completely lack spores produce a regular bipyramidal crystal inclusion. The oligosporogenic mutants kept the ability to produce insecticidal crystal proteins. However, sporulation in such mutants was not totally blocked and very few of them could still produce spores. In order to improve Bioinsecticides production, adaptation of sporeless strains to heat shock and osmotic stress was investigated. Delta-endotoxin production by 78% of sporeless mutants was significantly improved by osmotic stress with an overproduction of about 17%, compared to the wild strain BNS3. However, toxin production was improved by only 21% of mutants after heat shock, in low cost medium. The statistical analysis proved that delta-endotoxin production, cell growth, and spore formation of asporogenic and oligosporogenic mutants depended on the type of applied stress. Each strain has an important potential when applying the adequate stress. Moreover, adaptation of sporeless mutants to NaCl may allow the substitution of all minerals of the medium by diluted sea water which appeared to be a good alternative for the economic production of Bioinsecticides at industrial scale which is of great importance from the practical point of view.
Khoirul Ikhsanudin Hanif - One of the best experts on this subject based on the ideXlab platform.
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Efikasi Bioinsektisida Bacillus thuringiensis Barliner terhadap Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) pada Tanaman Padi Utama dan Ratun
2020Co-Authors: Khoirul Ikhsanudin Hanif, Siti Herlinda, Suwandi Suwandi, Tili KareninaAbstract:Hanif et al, 2017. Efficacy of Bacillus thuringiensis Barliner Bioinsecticide against Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) in Major Rice Crops and Ratooning Rice. JLSO 6(1):95-105. Crickets ( Gryllus bimaculatus ) are an important pests on rice that can reduce productivity. The application of Bacillus thuringiensis Bioinsecticide is expected to be an alternative methode to control these pest. The objective of the study was to investigate optimal dose of B. thuringiensis in suppressing rice damage was caused by G. bimaculatus . The observation were conducted on rice cultivation in Pelabuhan Dalam Village, Pemulutan Subdistrict of Ogan Ilir Regency. The results showed that application of B. thuringiensis Bioinsecticide had significant effects in suppressing rice crop damage by G. bimaculatus , but did not had significant effect on growth of rice plants.
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The impact of Bioinsecticide overdoses of Beauveria bassiana on species diversity and abundance of not targeted arthropods in South Sumatra (Indonesia) freshwater swamp paddy
Biodiversitas, 2020Co-Authors: Khoirul Ikhsanudin Hanif, Siti Herlinda, Chandra Irsan, Yulia Pujiastuti, Ghanni Prabawati, Hasbi, Tili KareninaAbstract:Abstract. Hanif KI, Herlinda S, Irsan C, Pujiastuti Y, Prabawati G, Hasbi, Karenina T. 2020. The impact of Bioinsecticide overdoses of Beauveria bassiana on species diversity and abundance of not targeted arthropods in South Sumatra (Indonesia) freshwater swamp paddy. Biodiversitas 21: 2124-2136. Bioinsecticides have been shown to reduce insect pest populations, but it is necessary to monitor the effect of Bioinsecticide overdoses on non-target arthropods because they are abundant in rice fields. This study aimed to analyze the impact of Bioinsecticide overdoses of B. bassiana on species diversity and abundance of non-target arthropods. Bioinsecticides derived from Beauveria bassiana were sprayed on the paddy every two weeks until the ripening stage, at a dose of 1, 2, 3, 4 L.ha-1, control (without insecticide), and abamectin as prescribed. The abundance of spiders and predatory arthropods did not decrease when Bioinsecticides were applied but the abundance of spiders, especially Lycosidae and Salticidae dropped significantly when abamectin was applied. The abundance of the parasitoid decreased significantly when abamectin was applied, but the abundance of parasitoids applied by Bioinsecticide was not significantly different from those of control. The abundance of insect pests started to decrease when Bioinsecticide was applied to 2, 3 or 4 L.ha-1. The diversity species of non-target arthropods (spiders, predatory arthropods, and parasitoids) tended to decrease when it was applied Bioinsecticides 3 and 4 L.ha-1 or abamectin, but their abundance remained high and no species dominance was found. For this reason, the correct dosage for suppressing insect pests abundance by not decreasing the abundance and diversity of non-target species is 2 L.ha-1 and applied when herbivore abundance is high.