The Experts below are selected from a list of 262239 Experts worldwide ranked by ideXlab platform
David Garon - One of the best experts on this subject based on the ideXlab platform.
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Comparison of tolerance and biosorption of three trace metals (Cd, Cu, Pb) by the soil fungus Absidia cylindrospora
Chemosphere, 2018Co-Authors: Quentin Albert, Lydia Leleyter, Melanie Lemoine, Natacha Heutte, Jean-philippe Rioult, Lucile Sage, Fabienne Baraud, David GaronAbstract:Trace metals cause deterioration of the soil and constitute a major concern for the environment and human health. Bioremediation could be an effective solution for the rectification of contaminated soils. Fungi could play an important role in biodegradation because of the morphology of their mycelium (highly reactive and extensive Biological Surface) and its physiology (high tolerance to many stresses, production of enzymes and secondary metabolites). Fungi can effectively biosequestrate, or biotransform many organic and inorganic contaminants into a non-bioavailable form. This experiment was designed to evaluate the tolerance and the biosorption abilities of the fungus Absidia cylindrospora against three trace metals: Cadmium (Cd), Copper (Cu), and Lead (Pb). Firstly, the tolerance of the strain was evaluated on metal-enriched malt extract agar (MEA). Secondly, the strain was exposed to trace metals, in a liquid malt extract medium. After 3 or 7 days of exposure, the quantities of absorbed and adsorbed metals were measured with Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). Biomass production and pH evolution were also evaluated during the test. Our experiment revealed differences between the three metals. In agar medium, Cd and Pb were better tolerated than Cu. In liquid medium, Cd and Pb were mostly absorbed whereas Cu was mostly adsorbed. A. cylindrospora biosorbed 14% of Cu, 59% of Pb and 68% of Cd when exposed for 3 days at 50 mg L−1.
Bengt Herbert Kasemo - One of the best experts on this subject based on the ideXlab platform.
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Biological Surface science
Surface Science, 2002Co-Authors: Bengt Herbert KasemoAbstract:Abstract Biological Surface science (BioSS), as defined here is the broad interdisciplinary area where properties and processes at interfaces between synthetic materials and Biological environments are investigated and biofunctional Surfaces are fabricated. Six examples are used to introduce and discuss the subject: Medical implants in the human body, biosensors and biochips for diagnostics, tissue engineering, bioelectronics, artificial photosynthesis, and biomimetic materials. They are areas of varying maturity, together constituting a strong driving force for the current rapid development of BioSS. The second driving force is the purely scientific challenges and opportunities to explore the mutual interaction between Biological components and Surfaces. Model systems range from the unique water structures at solid Surfaces and water shells around proteins and biomembranes, via amino and nucleic acids, proteins, DNA, phospholipid membranes, to cells and living tissue at Surfaces. At one end of the spectrum the scientific challenge is to map out the structures, bonding, dynamics and kinetics of biomolecules at Surfaces in a similar way as has been done for simple molecules during the past three decades in Surface science. At the other end of the complexity spectrum one addresses how biofunctional Surfaces participate in and can be designed to constructively participate in the total communication system of cells and tissue. Biofunctional Surfaces call for advanced design and preparation in order to match the sophisticated (bio) recognition ability of Biological systems. Specifically this requires combined topographic, chemical and visco-elastic patterns on Surfaces to match proteins at the nm scale and cells at the micrometer scale. Essentially all methods of Surface science are useful. High-resolution (e.g. scanning probe) microscopies, spatially resolved and high sensitivity, non-invasive optical spectroscopies, self-organizing monolayers, and nano- and microfabrication are important for BioSS. However, there is also a need to adopt or develop new methods for studies of biointerfaces in the native, liquid state. For the future it is likely that BioSS will have an even broader definition than above and include native interfaces, and that combinations of molecular (cell) biology and BioSS will contribute to the understanding of the “living state”.
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Biological Surface science
Current Opinion in Solid State & Materials Science, 1998Co-Authors: Bengt Herbert KasemoAbstract:Abstract Biological Surface science will be as important in the future of Surface science as semiconductors, catalysis and materials science have been in the past. There are three important research directions: firstly, studies of simple molecules such as water, amino acids, small peptides, and self-assembled monolayers of molecules both in UHV (ultrahigh vacuum) and in water. Secondly, studies of protein. DNA and membrane adsorption (kinetics, dynamics, structure and function) in the liquid phase. Thirdly, advanced preparation of biomaterial and biosensor Surfaces, by making Surface patterns with controlled chemical, topographic and micromechanical (viscoelastic) properties, for basic studies and applications such as, medical implants, biosensors, bioelectronics and tissue engineering.
Alexander Rich - One of the best experts on this subject based on the ideXlab platform.
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Biological Surface engineering a simple system for cell pattern formation
Biomaterials, 1999Co-Authors: Shuguang Zhang, Lin Yan, Michael D Altman, Michael Lassle, Helen Marie Nugent, Felice Frankel, Douglas A Lauffenburger, George M Whitesides, Alexander RichAbstract:Biological Surface engineering using synthetic Biological materials has a great potential for advances in our understanding of complex Biological phenomena. We developed a simple system to engineer Biologically relevant Surfaces using a combination of self-assembling oligopeptide monolayers and microcontact printing (kCP). We designed and synthesized two oligopeptides containing a cell adhesion motif (RADS) n (n"2 and 3) at the N-terminus, followed by an oligo(alanine) linker and a cysteine residue at the C-terminus. The thiol group of cysteine allows the oligopeptides to attach covalently onto a gold-coated Surface to form monolayers. We then microfabricated a variety of Surface patterns using the cell adhesion peptides in combination with hexa-ethylene glycol thiolate which resist non-speci"c adsorption of proteins and cells. The resulting patterns consist of areas either supporting or inhibiting cell adhesion, thus they are capable of aligning cells in a well-de"ned manner, leading to speci"c cell array and pattern formations. ( 1999 Elsevier Science Ltd. All rights reserved
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Biological Surface engineering a simple system for cell pattern formation
Biomaterials, 1999Co-Authors: Shuguang Zhang, Lin Yan, Michael D Altman, Michael Lassle, Helen Marie Nugent, Felice Frankel, Douglas A Lauffenburger, George M Whitesides, Alexander RichAbstract:Biological Surface engineering using synthetic Biological materials has a great potential for advances in our understanding of complex Biological phenomena. We developed a simple system to engineer Biologically relevant Surfaces using a combination of self-assembling oligopeptide monolayers and microcontact printing (muCP). We designed and synthesized two oligopeptides containing a cell adhesion motif (RADS)n (n = 2 and 3) at the N-terminus, followed by an oligo(alanine) linker and a cysteine residue at the C-terminus. The thiol group of cysteine allows the oligopeptides to attach covalently onto a gold-coated Surface to form monolayers. We then microfabricated a variety of Surface patterns using the cell adhesion peptides in combination with hexa-ethylene glycol thiolate which resist non-specific adsorption of proteins and cells. The resulting patterns consist of areas either supporting or inhibiting cell adhesion, thus they are capable of aligning cells in a well-defined manner, leading to specific cell array and pattern formations.
Quentin Albert - One of the best experts on this subject based on the ideXlab platform.
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Comparison of tolerance and biosorption of three trace metals (Cd, Cu, Pb) by the soil fungus Absidia cylindrospora
Chemosphere, 2018Co-Authors: Quentin Albert, Lydia Leleyter, Melanie Lemoine, Natacha Heutte, Jean-philippe Rioult, Lucile Sage, Fabienne Baraud, David GaronAbstract:Trace metals cause deterioration of the soil and constitute a major concern for the environment and human health. Bioremediation could be an effective solution for the rectification of contaminated soils. Fungi could play an important role in biodegradation because of the morphology of their mycelium (highly reactive and extensive Biological Surface) and its physiology (high tolerance to many stresses, production of enzymes and secondary metabolites). Fungi can effectively biosequestrate, or biotransform many organic and inorganic contaminants into a non-bioavailable form. This experiment was designed to evaluate the tolerance and the biosorption abilities of the fungus Absidia cylindrospora against three trace metals: Cadmium (Cd), Copper (Cu), and Lead (Pb). Firstly, the tolerance of the strain was evaluated on metal-enriched malt extract agar (MEA). Secondly, the strain was exposed to trace metals, in a liquid malt extract medium. After 3 or 7 days of exposure, the quantities of absorbed and adsorbed metals were measured with Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). Biomass production and pH evolution were also evaluated during the test. Our experiment revealed differences between the three metals. In agar medium, Cd and Pb were better tolerated than Cu. In liquid medium, Cd and Pb were mostly absorbed whereas Cu was mostly adsorbed. A. cylindrospora biosorbed 14% of Cu, 59% of Pb and 68% of Cd when exposed for 3 days at 50 mg L−1.
Shuguang Zhang - One of the best experts on this subject based on the ideXlab platform.
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Biological Surface engineering a simple system for cell pattern formation
Biomaterials, 1999Co-Authors: Shuguang Zhang, Lin Yan, Michael D Altman, Michael Lassle, Helen Marie Nugent, Felice Frankel, Douglas A Lauffenburger, George M Whitesides, Alexander RichAbstract:Biological Surface engineering using synthetic Biological materials has a great potential for advances in our understanding of complex Biological phenomena. We developed a simple system to engineer Biologically relevant Surfaces using a combination of self-assembling oligopeptide monolayers and microcontact printing (kCP). We designed and synthesized two oligopeptides containing a cell adhesion motif (RADS) n (n"2 and 3) at the N-terminus, followed by an oligo(alanine) linker and a cysteine residue at the C-terminus. The thiol group of cysteine allows the oligopeptides to attach covalently onto a gold-coated Surface to form monolayers. We then microfabricated a variety of Surface patterns using the cell adhesion peptides in combination with hexa-ethylene glycol thiolate which resist non-speci"c adsorption of proteins and cells. The resulting patterns consist of areas either supporting or inhibiting cell adhesion, thus they are capable of aligning cells in a well-de"ned manner, leading to speci"c cell array and pattern formations. ( 1999 Elsevier Science Ltd. All rights reserved
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Biological Surface engineering a simple system for cell pattern formation
Biomaterials, 1999Co-Authors: Shuguang Zhang, Lin Yan, Michael D Altman, Michael Lassle, Helen Marie Nugent, Felice Frankel, Douglas A Lauffenburger, George M Whitesides, Alexander RichAbstract:Biological Surface engineering using synthetic Biological materials has a great potential for advances in our understanding of complex Biological phenomena. We developed a simple system to engineer Biologically relevant Surfaces using a combination of self-assembling oligopeptide monolayers and microcontact printing (muCP). We designed and synthesized two oligopeptides containing a cell adhesion motif (RADS)n (n = 2 and 3) at the N-terminus, followed by an oligo(alanine) linker and a cysteine residue at the C-terminus. The thiol group of cysteine allows the oligopeptides to attach covalently onto a gold-coated Surface to form monolayers. We then microfabricated a variety of Surface patterns using the cell adhesion peptides in combination with hexa-ethylene glycol thiolate which resist non-specific adsorption of proteins and cells. The resulting patterns consist of areas either supporting or inhibiting cell adhesion, thus they are capable of aligning cells in a well-defined manner, leading to specific cell array and pattern formations.