The Experts below are selected from a list of 26268 Experts worldwide ranked by ideXlab platform
Caroline Montagnani - One of the best experts on this subject based on the ideXlab platform.
-
transcriptome and proteome analysis of pinctada margaritifera calcifying mantle and shell focus on Biomineralization
BMC Genomics, 2010Co-Authors: Caroline Joubert, David Piquemal, Benjamin Marie, Laurent Manchon, Fabien Pierrat, Yannick Gueguen, Isabelle Zanellacleon, Nathalie Cochenneclaureau, Caroline MontagnaniAbstract:The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled Biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in Biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera Biomineralization-related mantle protein sequences. Components of the current "chitin-silk fibroin gel-acidic macromolecule" model of Biomineralization processes were found, in particular a homolog of a Biomineralization protein (Pif-177) recently discovered in P. fucata. Among these sequences, we could show the localization of two other Biomineralization protein transcripts, pmarg-aspein and pmarg-pearlin, in two distinct areas of the outer mantle epithelium, suggesting their implication in calcite and aragonite formation. Finally, by combining the EST approach with a proteomic mass spectrometry analysis of proteins isolated from the P. margaritifera shell organic matrix, we demonstrated the presence of 30 sequences containing almost all of the shell proteins that have been previously described from shell matrix protein analyses of the Pinctada genus. The integration of these two methods allowed the global composition of biomineralizing tissue and calcified structures to be examined in tandem for the first time. This EST study made on the calcifying tissue of P. margaritifera is the first description of pyrosequencing on a pearl-producing bivalve species. Our results provide direct evidence that our EST data set covers most of the diversity of the matrix protein of P. margaritifera shell, but also that the mantle transcripts encode proteins present in P. margaritifera shell, hence demonstrating their implication in shell formation. Combining transcriptomic and proteomic approaches is therefore a powerful way to identify proteins involved in Biomineralization. Data generated in this study supply the most comprehensive list of Biomineralization-related sequences presently available among protostomian species, and represent a major breakthrough in the field of molluskan Biomineralization.
-
Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on Biomineralization.
BMC Genomics, 2010Co-Authors: Caroline Joubert, David Piquemal, Benjamin Marie, Laurent Manchon, Fabien Pierrat, Isabelle Zanella-cléon, Nathalie Cochennec-laureau, Yannick Gueguen, Caroline MontagnaniAbstract:BACKGROUND: The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled Biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in Biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. RESULTS: We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera Biomineralization-related mantle protein sequences. Components of the current "chitin-silk fibroin gel-acidic macromolecule" model of Biomineralization processes were found, in particular a homolog of a Biomineralization protein (Pif-177) recently discovered in P. fucata. Among these sequences, we could show the localization of two other Biomineralization protein transcripts, pmarg-aspein and pmarg-pearlin, in two distinct areas of the outer mantle epithelium, suggesting their implication in calcite and aragonite formation. Finally, by combining the EST approach with a proteomic mass spectrometry analysis of proteins isolated from the P. margaritifera shell organic matrix, we demonstrated the presence of 30 sequences containing almost all of the shell proteins that have been previously described from shell matrix protein analyses of the Pinctada genus. The integration of these two methods allowed the global composition of biomineralizing tissue and calcified structures to be examined in tandem for the first time. CONCLUSIONS: This EST study made on the calcifying tissue of P. margaritifera is the first description of pyrosequencing on a pearl-producing bivalve species. Our results provide direct evidence that our EST data set covers most of the diversity of the matrix protein of P. margaritifera shell, but also that the mantle transcripts encode proteins present in P. margaritifera shell, hence demonstrating their implication in shell formation. Combining transcriptomic and proteomic approaches is therefore a powerful way to identify proteins involved in Biomineralization. Data generated in this study supply the most comprehensive list of Biomineralization-related sequences presently available among protostomian species, and represent a major breakthrough in the field of molluskan Biomineralization.
Virgile Quillien - One of the best experts on this subject based on the ideXlab platform.
-
Tracing key genes associated with the Pinctada margaritifera albino phenotype from juvenile to cultured pearl harvest stages using multiple whole transcriptome sequencing
BMC Genomics, 2020Co-Authors: Pauline Auffret, Jérémy Le Luyer, Manaarii Sham Koua, Virgile QuillienAbstract:Background Albino mutations are commonly observed in the animal kingdom, including in bivalves. In the black-lipped pearl oyster Pinctada margaritifera, albino specimens are characterized by total or partial absence of colouration resulting in typical white shell phenotype expression. The relationship of shell colour with resulting cultured pearl colour is of great economic interest in P. margaritifera, on which a pearl industry is based. Hence, the albino phenotype provides a useful way to examine the molecular mechanisms underlying pigmentation. Results Whole transcriptome RNA-sequencing analysis comparing albino and black wild-type phenotypes at three stages over the culture cycle of P. margaritifera revealed a total of 1606, 798 and 187 differentially expressed genes in whole juvenile, adult mantle and pearl sac tissue, respectively. These genes were found to be involved in five main molecular pathways, tightly linked to known pigmentation pathways: melanogenesis, calcium signalling pathway, Notch signalling pathway, pigment transport and Biomineralization. Additionally, significant phenotype-associated SNPs were selected ( N = 159), including two located in the Pif Biomineralization gene, which codes for nacre formation. Interestingly, significantly different transcript splicing was detected between juvenile ( N = 1366) and adult mantle tissue ( N = 313) in, e.g., the tyrosinase Tyr-1 gene, which showed more complex regulation in mantle, and the Notch1 encoding gene, which was upregulated in albino juveniles. Conclusion This multiple RNA-seq approach provided new knowledge about genes associated with the P. margaritifera albino phenotype, highlighting: 1) new molecular pathways, such as the Notch signalling pathway in pigmentation, 2) associated SNP markers with biomineraliszation gene of interest like Pif for marker-assisted selection and prevention of inbreeding, and 3) alternative gene splicing for melanin biosynthesis implicating tyrosinase.
Denis Saulnier - One of the best experts on this subject based on the ideXlab platform.
-
Influence of preoperative food and temperature conditions on pearl biogenesis in Pinctada margaritifera
Aquaculture, 2017Co-Authors: Oihana Latchere, Nabila Gaertner-mazouni, Kevin Magré, Gilles Le Moullac, Julie Fievet, Denis SaulnierAbstract:Trophic conditions and water temperature strongly influence bivalve physiological processes and metabolism. In black-lip pearl oyster Pinctada margaritifera, these parameters have been shown to affect shell Biomineralization. The present study investigated the effect of preoperative food level (i.e., microalgal concentration) and temperature on pearl Biomineralization. Donor and recipient oysters were conditioned at different levels of food and temperature during the preoperative phase to evaluate the influence of these factors on 1) pearl retention rate (grafting success), 2) expression of genes involved in Biomineralization in the mantle and pearl sac and 3) pearl quality traits. Our study confirmed the influence of both microalgal concentration and temperature on shell growth. Food level of donor oysters was decisive for pearl Biomineralization, with donors that had been fed at a high microalgal concentration producing pearl sacs with significantly higher Biomineralization capabilities and faster nacre establishment during early stages of pearl formation. However, food level showed no effects on quality traits of the pearls harvested 12 months postgrafting, while preoperative temperature only influenced the relative expression of two genes in pearl sacs at 12 months postgrafting. No significant effects of the preoperative conditioning of recipient oysters were detected in either experiment considering gene expression measurements and pearl quality traits. However, mortality was significantly lower in grafted recipient oysters fed at an intermediate trophic level. Finally, pearl weight was shown to be positively correlated with recipient oyster growth.
Caroline Joubert - One of the best experts on this subject based on the ideXlab platform.
-
transcriptome and proteome analysis of pinctada margaritifera calcifying mantle and shell focus on Biomineralization
BMC Genomics, 2010Co-Authors: Caroline Joubert, David Piquemal, Benjamin Marie, Laurent Manchon, Fabien Pierrat, Yannick Gueguen, Isabelle Zanellacleon, Nathalie Cochenneclaureau, Caroline MontagnaniAbstract:The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled Biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in Biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera Biomineralization-related mantle protein sequences. Components of the current "chitin-silk fibroin gel-acidic macromolecule" model of Biomineralization processes were found, in particular a homolog of a Biomineralization protein (Pif-177) recently discovered in P. fucata. Among these sequences, we could show the localization of two other Biomineralization protein transcripts, pmarg-aspein and pmarg-pearlin, in two distinct areas of the outer mantle epithelium, suggesting their implication in calcite and aragonite formation. Finally, by combining the EST approach with a proteomic mass spectrometry analysis of proteins isolated from the P. margaritifera shell organic matrix, we demonstrated the presence of 30 sequences containing almost all of the shell proteins that have been previously described from shell matrix protein analyses of the Pinctada genus. The integration of these two methods allowed the global composition of biomineralizing tissue and calcified structures to be examined in tandem for the first time. This EST study made on the calcifying tissue of P. margaritifera is the first description of pyrosequencing on a pearl-producing bivalve species. Our results provide direct evidence that our EST data set covers most of the diversity of the matrix protein of P. margaritifera shell, but also that the mantle transcripts encode proteins present in P. margaritifera shell, hence demonstrating their implication in shell formation. Combining transcriptomic and proteomic approaches is therefore a powerful way to identify proteins involved in Biomineralization. Data generated in this study supply the most comprehensive list of Biomineralization-related sequences presently available among protostomian species, and represent a major breakthrough in the field of molluskan Biomineralization.
-
Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on Biomineralization.
BMC Genomics, 2010Co-Authors: Caroline Joubert, David Piquemal, Benjamin Marie, Laurent Manchon, Fabien Pierrat, Isabelle Zanella-cléon, Nathalie Cochennec-laureau, Yannick Gueguen, Caroline MontagnaniAbstract:BACKGROUND: The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled Biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in Biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. RESULTS: We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera Biomineralization-related mantle protein sequences. Components of the current "chitin-silk fibroin gel-acidic macromolecule" model of Biomineralization processes were found, in particular a homolog of a Biomineralization protein (Pif-177) recently discovered in P. fucata. Among these sequences, we could show the localization of two other Biomineralization protein transcripts, pmarg-aspein and pmarg-pearlin, in two distinct areas of the outer mantle epithelium, suggesting their implication in calcite and aragonite formation. Finally, by combining the EST approach with a proteomic mass spectrometry analysis of proteins isolated from the P. margaritifera shell organic matrix, we demonstrated the presence of 30 sequences containing almost all of the shell proteins that have been previously described from shell matrix protein analyses of the Pinctada genus. The integration of these two methods allowed the global composition of biomineralizing tissue and calcified structures to be examined in tandem for the first time. CONCLUSIONS: This EST study made on the calcifying tissue of P. margaritifera is the first description of pyrosequencing on a pearl-producing bivalve species. Our results provide direct evidence that our EST data set covers most of the diversity of the matrix protein of P. margaritifera shell, but also that the mantle transcripts encode proteins present in P. margaritifera shell, hence demonstrating their implication in shell formation. Combining transcriptomic and proteomic approaches is therefore a powerful way to identify proteins involved in Biomineralization. Data generated in this study supply the most comprehensive list of Biomineralization-related sequences presently available among protostomian species, and represent a major breakthrough in the field of molluskan Biomineralization.
Pauline Auffret - One of the best experts on this subject based on the ideXlab platform.
-
Tracing key genes associated with the Pinctada margaritifera albino phenotype from juvenile to cultured pearl harvest stages using multiple whole transcriptome sequencing
BMC Genomics, 2020Co-Authors: Pauline Auffret, Jérémy Le Luyer, Manaarii Sham Koua, Virgile QuillienAbstract:Background Albino mutations are commonly observed in the animal kingdom, including in bivalves. In the black-lipped pearl oyster Pinctada margaritifera, albino specimens are characterized by total or partial absence of colouration resulting in typical white shell phenotype expression. The relationship of shell colour with resulting cultured pearl colour is of great economic interest in P. margaritifera, on which a pearl industry is based. Hence, the albino phenotype provides a useful way to examine the molecular mechanisms underlying pigmentation. Results Whole transcriptome RNA-sequencing analysis comparing albino and black wild-type phenotypes at three stages over the culture cycle of P. margaritifera revealed a total of 1606, 798 and 187 differentially expressed genes in whole juvenile, adult mantle and pearl sac tissue, respectively. These genes were found to be involved in five main molecular pathways, tightly linked to known pigmentation pathways: melanogenesis, calcium signalling pathway, Notch signalling pathway, pigment transport and Biomineralization. Additionally, significant phenotype-associated SNPs were selected ( N = 159), including two located in the Pif Biomineralization gene, which codes for nacre formation. Interestingly, significantly different transcript splicing was detected between juvenile ( N = 1366) and adult mantle tissue ( N = 313) in, e.g., the tyrosinase Tyr-1 gene, which showed more complex regulation in mantle, and the Notch1 encoding gene, which was upregulated in albino juveniles. Conclusion This multiple RNA-seq approach provided new knowledge about genes associated with the P. margaritifera albino phenotype, highlighting: 1) new molecular pathways, such as the Notch signalling pathway in pigmentation, 2) associated SNP markers with biomineraliszation gene of interest like Pif for marker-assisted selection and prevention of inbreeding, and 3) alternative gene splicing for melanin biosynthesis implicating tyrosinase.