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Carlos Ricardo Soccol - One of the best experts on this subject based on the ideXlab platform.
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pulp improvement of oil palm empty fruit bunches associated to solid state Biopulping and biobleaching with xylanase and lignin peroxidase cocktail produced by aspergillus sp lpb 5
Bioresource Technology, 2019Co-Authors: Brigitte Sthepani Orozco Colonia, Carlos Ricardo Soccol, Luiz Alberto Junior Letti, Adenise Lorenci Woiciechowski, Rodrigo MalanskiAbstract:Abstract Oil palm empty fruit bunches is a lignocellulosic feedstock with biotechnological potential and thousands of tons are generated in the world each year. Filamentous fungi producing xylanases and ligninases in Biopulping to obtain cellulose is a pulp improvement alternative. The enzymatic cocktail was produced in solid-state Biopulping by Aspergillus sp. LPB-5 with 54.32 U/g xylanase, 13.41 U/g lignin peroxidase and low cellulase activity. Biological, thermal and chemical pretreatments were compared and enzymatic biobleaching was applied to pretreated pulps. Biopulping and biobleaching combination had 36.80% lignin loss, 26.27% hemicellulose reduction, 74.36% pulp yield with 36.56% digestibility. Alkaline and biobleaching combination removed 81.97% hemicellulose and 93.89% lignin with 73.59% digestibility. Enzymatic biobleaching increased the pulp digestibility in all pretreatments. Finally, the development of a bio-pretreatment to remove hemicellulose and alter the lignin-carbohydrate complex interface presented a soft process with great eco-friendly potential, where mild pre-treatments would reduce the use of aggressive agents.
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recent developments and innovations in solid state fermentation
Biotechnology Research and Innovation, 2017Co-Authors: Carlos Ricardo Soccol, Eduardo Scopel Ferreira Da Costa, Luiz Alberto Junior Letti, Susan Grace Karp, Adenise Lorenci Woiciechowski, Luciana Porto De Souza VandenbergheAbstract:After forty years of research development, an overview of solid-state fermentation (SSF), focusing on its applications, mainly of the very recent papers of the last five years, is presented. This review comprises the most important developed processes concerning the production of enzymes, Biopulping processes, and traditional processes, for food fermentation, such as the production of Chinese daqu and koji, and industrial important biomolecules such as organic acids, pigments, phenolic compounds, aromas and biosorbents. SSF bioreactors design that has been developed is reported, so as the solutions for the classical drawbacks and the most important cases of successful employment of the technique are described. And, finally, it is summarized a very interesting report of patents and innovations regarding SSF products and processes.
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Recent developments and innovations in solid state fermentation
Elsevier, 2017Co-Authors: Carlos Ricardo Soccol, Eduardo Scopel Ferreira Da Costa, Luiz Alberto Junior Letti, Susan Grace Karp, Adenise Lorenci Woiciechowski, Luciana Porto De Souza VandenbergheAbstract:After forty years of research development, an overview of solid-state fermentation (SSF), focusing on its applications, mainly of the very recent papers of the last five years, is presented. This review comprises the most important developed processes concerning the production of enzymes, Biopulping processes, and traditional processes, for food fermentation, such as the production of Chinese daqu and koji, and industrial important biomolecules such as organic acids, pigments, phenolic compounds, aromas and biosorbents. SSF bioreactors design that has been developed is reported, so as the solutions for the classical drawbacks and the most important cases of successful employment of the technique are described. And, finally, it is summarized a very interesting report of patents and innovations regarding SSF products and processes. Keywords: Solid-state fermentation, Bioreactors, Patents, Agro-industrial substrate
Annele Hatakka - One of the best experts on this subject based on the ideXlab platform.
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heterologous expression and structural characterization of two low ph laccases from a Biopulping white rot fungus physisporinus rivulosus
Applied Microbiology and Biotechnology, 2013Co-Authors: Taina Lundell, Kristiina Hilden, Miia R Makela, Jaana Kuuskeri, A M Chernykh, L A Golovleva, David B Archer, Annele HatakkaAbstract:The lignin-degrading, Biopulping white-rot fungus Physisporinus rivulosus secretes several laccases of distinct features such as thermostability, extremely low pH optima and thermal activation for oxidation of phenolic substrates. Here we describe the cloning, heterologous expression and structural and enzymatic characterisation of two previously undescribed P. rivulosus laccases. The laccase cDNAs were expressed in the methylotrophic yeast Pichia pastoris either with the native or with Saccharomyces cerevisiae α-factor signal peptide. The specific activity of rLac1 and rLac2 was 5 and 0.3 μkat/μg, respectively. However, mutation of the last amino acid in the rLac2 increased the specific laccase activity by over 50-fold. The recombinant rLac1 and rLac2 enzymes demonstrated low pH optima with both 2,6-dimethoxyphenol (2,6-DMP) and 2,2′-azino-bis(3-ethylbenzathiazoline-6-sulfonate). Both recombinant laccases showed moderate thermotolerance and thermal activation at +60 °C was detected with rLac1. By homology modelling, it was deduced that Lac1 and Lac2 enzymes demonstrate structural similarity with the Trametes versicolor and Trametes trogii laccase crystal structures. Comparison of the protein architecture at the reducing substrate-binding pocket near the T1-Cu site indicated the presence of five amino acid substitutions in the structural models of Lac1 and Lac2. These data add up to our previous reports on laccase production by P. rivulosus during Biopulping and growth on Norway spruce. Heterologous expression of the novel Lac1 and Lac2 isoenzymes in P. pastoris enables the detailed study of their properties and the evaluation of their potential as oxidative biocatalysts for conversion of wood lignin, lignin-like compounds and soil-polluting xenobiotics.
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can co culturing of two white rot fungi increase lignin degradation and the production of lignin degrading enzymes
International Biodeterioration & Biodegradation, 2007Co-Authors: Yujie Chi, Annele Hatakka, Pekka MaijalaAbstract:The aim of this work was to investigate the poorly understood effects of co-culturing of two white rot fungi on the production of lignin-degrading enzyme activities. Four species, Ceriporiopsis subvermispora, Physisporinus rivulosus, Phanerochaete chrysosporium and Pleurotus ostreatus were cultured in pairs to study the degradation of aspen wood and the production of lignin-degrading enzymes. Potential of co-culturing for Biopulping was evaluated. Chemical analysis of decayed aspen wood blocks showed that co-culturing of C. subvermispora with P. ostreatus could significantly stimulate wood decay, when compared to monocultures. Based on the fungi tested here, however, this effect is species-specific. Other combinations of fungi were slightly stimulating or not stimulatory. The pattern of lignin degradation was altered towards the acid insoluble part of lignin especially in co-cultures where P. ostreatus was included as a partner. The use of agar plates containing the polymeric dye Poly R-478 showed elevated dye decolourization at the confrontation zone between mycelia. Laccase was significantly stimulated only in the co-culture of P. ostreatus with C. subvermispora. Manganese peroxidase activity was stimulated in co-cultures of P. ostreatus with C. subvermispora or with P. rivulosus. Immunoblotting indicated changes in lignin-degrading enzymes and/or their isoform composition in response to co-culturing. This is the first report on the effects of co-culturing of potential Biopulping fungi on wood degradation, and gives basic knowledge on fungal interactions during wood decay that can be utilized in practical applications.
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manganese peroxidases laccases and oxalic acid from the selective white rot fungus physisporinus rivulosus grown on spruce wood chips
Enzyme and Microbial Technology, 2005Co-Authors: Terhi K Hakala, Pekka Maijala, Taina Lundell, Sari Galkin, Nisse Kalkkinen, Annele HatakkaAbstract:Abstract The white-rot fungus Physisporinus rivulosus T241i produced manganese peroxidase (MnP), laccase, and oxalic acid when it was grown on spruce ( Picea abies ) wood chips. This white-rot basidiomycete degrades lignin selectively and is promising for use in Biopulping of softwood. During solid-state cultivation on wood chips the fungus produced oxalic acid (28 μmol/g dry wood) and a high MnP activity (24 nkat/g dry wood), while measured laccase activity was negligible. The prepurified laccase and MnP isoforms were fractionated with anion exchange chromatography. Characterization of the isolated MnP fractions indicated the presence of several MnP isoforms with p I values between 3.4 and 3.9 and molecular masses between 47 and 52 kDa. The four laccase isoforms had p I values between 3.1 and 3.3 and molecular masses between 66 and 68 kDa. The MnP and laccase isoform profiles did not vary during the 4-week cultivation. The N-terminal amino acid sequences of four laccase and two MnP isoenzymes resembled those of the two other selective white-rot fungi applied in Biopulping, i.e. Ceriporiopsis subvermispora CZ-3 and the strain IZU-154.
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evaluation of novel wood rotting polypores and corticioid fungi for the decay and Biopulping of norway spruce picea abies wood
Enzyme and Microbial Technology, 2004Co-Authors: Terhi K Hakala, Pekka Maijala, Jonas Konn, Annele HatakkaAbstract:Abstract Pre-treatment of wood chips with white-rot fungi (i.e. Biopulping) has previously been shown to decrease the energy consumption in mechanical pulping. In this study, promising novel fungal isolates were found for Biopulping of Norway spruce ( Picea abies ). Three hundred fungal isolates were evaluated by agar plate tests. According to the results, 86 isolates were chosen to a wood block decay test. The characteristics of the fungal attack and the wood decay were studied and chemical analyses of cellulose, lignin, and hemicelluloses in the residual wood were performed. The results showed both interspecies and intraspecies variations in the degradation patterns of these compounds. After a 10-week incubation period, 17 of the novel white-rot strains showed selective lignin degrading ability, based on the chemical analyses. Some of these lignin-selective strains also grew well even at +37 °C. The selectivity value, i.e. the lignin/cellulose loss ratio, was higher for 11 of the studied strains compared to the widely studied Ceriporiopsis subvermispora . In this study, Physisporinus rivulosus T241i was shown to be a superior novel isolate for softwood Biopulping applications based on its growth characteristics through a wide temperature range and its selectivity towards lignin.
Pedro Dario Zapata - One of the best experts on this subject based on the ideXlab platform.
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Biopulping of wood chips with phlebia brevispora bafc 633 reduces lignin content and improves pulp quality
International Biodeterioration & Biodegradation, 2014Co-Authors: Maria Isabel Fonseca, Julia Ines Farina, Maria Lorena Castrillo, Maria Daniela Rodriguez, Carlos Eduardo Nunez, Laura Lidia Villalba, Pedro Dario ZapataAbstract:Abstract The white-rot fungus Phlebia brevispora BAFC 633 produces laccases in large proportions. In this work P. brevispora BAFC 633 was grown on Pinus taeda wood chips in 10-L bioreactors. To select the Biopulping experimental conditions, we analyzed the variables affecting enzymatic laccase activity in the culture supernatants, indicating that the suitable incubation temperature was 30 °C in order to promote enzyme stability. Phlebia brevispora BAFC 633 secreted 744 U/g of laccase, selectively removing lignin during biotreatment of wood chips, causing a reduction in Kappa number and 10% weight loss, and creating a more open structure and better access to the pulping liquor, which would require less chemical consumption, thus diminishing the environmental impact of the chemical pulping process. These results support the biotechnological potential of P. brevispora BAFC 633 for Biopulping processes and enhance the potential for bioprospecting native isolates of the microflora of our country's natural environment.
Maria Isabel Fonseca - One of the best experts on this subject based on the ideXlab platform.
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Biopulping of wood chips with phlebia brevispora bafc 633 reduces lignin content and improves pulp quality
International Biodeterioration & Biodegradation, 2014Co-Authors: Maria Isabel Fonseca, Julia Ines Farina, Maria Lorena Castrillo, Maria Daniela Rodriguez, Carlos Eduardo Nunez, Laura Lidia Villalba, Pedro Dario ZapataAbstract:Abstract The white-rot fungus Phlebia brevispora BAFC 633 produces laccases in large proportions. In this work P. brevispora BAFC 633 was grown on Pinus taeda wood chips in 10-L bioreactors. To select the Biopulping experimental conditions, we analyzed the variables affecting enzymatic laccase activity in the culture supernatants, indicating that the suitable incubation temperature was 30 °C in order to promote enzyme stability. Phlebia brevispora BAFC 633 secreted 744 U/g of laccase, selectively removing lignin during biotreatment of wood chips, causing a reduction in Kappa number and 10% weight loss, and creating a more open structure and better access to the pulping liquor, which would require less chemical consumption, thus diminishing the environmental impact of the chemical pulping process. These results support the biotechnological potential of P. brevispora BAFC 633 for Biopulping processes and enhance the potential for bioprospecting native isolates of the microflora of our country's natural environment.
Bolhassan Mohamad Hasnul - One of the best experts on this subject based on the ideXlab platform.
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Diversity of polyporales and the application of Ganoderma Australe (fr.) pat. in Biopulping of empty fruit bunches of Elaeis Guineensis / Mohamad Hasnul Bolhassan
2013Co-Authors: Bolhassan Mohamad HasnulAbstract:Diversity and distribution of Polyporales in Malaysia was investigated by collecting basidiocarps from trunks, branches, exposed roots and soil from six states (Johore, Kedah, Kelantan, Negeri Sembilan, Pahang and Selangor) in Peninsular Malaysia and Federal Territory Kuala Lumpur. The morphological study of 99 basidiomata collected from 2006 till 2007 and 241 herbarium specimens collected from 2003 - 2005 were undertaken. Sixty species belonging to five families: Fomitopsidaceae, Ganodermataceae, Meruliaceae, Meripilaceae and Polyporaceae were recorded. Polyporaceae was the dominant family with 46 species identified. The common species encountered based on the number of basidiocarps collected were Ganoderma australe followed by Lentinus squarrosulus, Earliella scabrosa, Pycnoporus sanguineus, Lentinus connatus, Microporus xanthopus, Trametes menziesii, Lenzites elegans, Lentinus sajor-caju and Microporus affinis. Eighteen genera with only one specie were also recorded i.e. Daedalea, Amauroderma, Flavodon, Earliella, Echinochaetae, Favolus, Flabellophora, Fomitella, Funalia, Hexagonia, Lignosus, Macrohyporia, Microporellus, Nigroporus, Panus, Perenniporia, Pseudofavolus and Pyrofomes. This study shows that strains of the G. lucidum and G. australe can be identified by 650 base pair nucleic acid sequence characters from ITS1, 5.8S rDNA and ITS2 region on the ribosomal DNA. The phylogenetic analysis used maximum-parsimony as the optimality criterion and heuristic searches used 100 replicates of random addition sequences with tree-bisection-reconnection (TBR) branch-swaping. ITS phylogeny confirms that G. lucidum and G. australe were named correctly based on the molecular analysis even though the strains exhibited differences in morphological characteristics. Thirty-seven selected cultures of Polyporales were qualitatively assayed for the production of amylases, cellulases, laccases and lignin peroxidases after three to seven days incubation at 25±2°C. Two strains - Ganoderma australe KUM60848 and Favolus iii tenuiculus KUM60803 demonstrated good enzymes production and were selected to undergo solid substrate fermentation of oil palm empty fruit bunches (EFB). The study was conducted to analyse the enzymatic activity (U/ml) of cellulase, amylase, laccase, lignin peroxidase, xylanase and β-D-glucosidase. Ganoderma australe showed the highest enzymes activity on the 14 and 21 days of incubation compared to F. tenuiculus and was selected as potential candidate for Biopulping of oil palm (Elaeis guineensis) empty fruit bunches. The property of pulp produced by oil palm empty fruit bunches through solid substrate fermentation with G. australe KUM60848 were then analysed at 14 and 21 days of incubation. The empty fruit bunches was pulped by applying soda pulping process. The result showed that the pulping process influenced the pulping properties. Pre-treatment by G. australe for 14 days produced the lowest degree of material dissolved while pre-treatment at 21 days had the highest degree of material dissolved as indicated by the pulp yields. Compared to control, the Biopulping yield using G. australe had increased to a maximum of 18%. The pulping process also influenced the paper properties i.e. all zero-span tensile indices of pulp were lower than control (conventional pulping), while the fibre strength decreased by 11% and 6% at day 14 and 21 respectively. In conclusion, the 14 days of solid substrate fermentation by G. australe performs better pulp and paper properties than 21 days in Biopulping of EFB.
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diversity of polyporales and the application of ganoderma australe fr pat in Biopulping of empty fruit bunches of elaeis guineensis mohamad hasnul bolhassan
2013Co-Authors: Bolhassan Mohamad HasnulAbstract:Diversity and distribution of Polyporales in Malaysia was investigated by collecting basidiocarps from trunks, branches, exposed roots and soil from six states (Johore, Kedah, Kelantan, Negeri Sembilan, Pahang and Selangor) in Peninsular Malaysia and Federal Territory Kuala Lumpur. The morphological study of 99 basidiomata collected from 2006 till 2007 and 241 herbarium specimens collected from 2003 - 2005 were undertaken. Sixty species belonging to five families: Fomitopsidaceae, Ganodermataceae, Meruliaceae, Meripilaceae and Polyporaceae were recorded. Polyporaceae was the dominant family with 46 species identified. The common species encountered based on the number of basidiocarps collected were Ganoderma australe followed by Lentinus squarrosulus, Earliella scabrosa, Pycnoporus sanguineus, Lentinus connatus, Microporus xanthopus, Trametes menziesii, Lenzites elegans, Lentinus sajor-caju and Microporus affinis. Eighteen genera with only one specie were also recorded i.e. Daedalea, Amauroderma, Flavodon, Earliella, Echinochaetae, Favolus, Flabellophora, Fomitella, Funalia, Hexagonia, Lignosus, Macrohyporia, Microporellus, Nigroporus, Panus, Perenniporia, Pseudofavolus and Pyrofomes. This study shows that strains of the G. lucidum and G. australe can be identified by 650 base pair nucleic acid sequence characters from ITS1, 5.8S rDNA and ITS2 region on the ribosomal DNA. The phylogenetic analysis used maximum-parsimony as the optimality criterion and heuristic searches used 100 replicates of random addition sequences with tree-bisection-reconnection (TBR) branch-swaping. ITS phylogeny confirms that G. lucidum and G. australe were named correctly based on the molecular analysis even though the strains exhibited differences in morphological characteristics. Thirty-seven selected cultures of Polyporales were qualitatively assayed for the production of amylases, cellulases, laccases and lignin peroxidases after three to seven days incubation at 25±2°C. Two strains - Ganoderma australe KUM60848 and Favolus iii tenuiculus KUM60803 demonstrated good enzymes production and were selected to undergo solid substrate fermentation of oil palm empty fruit bunches (EFB). The study was conducted to analyse the enzymatic activity (U/ml) of cellulase, amylase, laccase, lignin peroxidase, xylanase and β-D-glucosidase. Ganoderma australe showed the highest enzymes activity on the 14 and 21 days of incubation compared to F. tenuiculus and was selected as potential candidate for Biopulping of oil palm (Elaeis guineensis) empty fruit bunches. The property of pulp produced by oil palm empty fruit bunches through solid substrate fermentation with G. australe KUM60848 were then analysed at 14 and 21 days of incubation. The empty fruit bunches was pulped by applying soda pulping process. The result showed that the pulping process influenced the pulping properties. Pre-treatment by G. australe for 14 days produced the lowest degree of material dissolved while pre-treatment at 21 days had the highest degree of material dissolved as indicated by the pulp yields. Compared to control, the Biopulping yield using G. australe had increased to a maximum of 18%. The pulping process also influenced the paper properties i.e. all zero-span tensile indices of pulp were lower than control (conventional pulping), while the fibre strength decreased by 11% and 6% at day 14 and 21 respectively. In conclusion, the 14 days of solid substrate fermentation by G. australe performs better pulp and paper properties than 21 days in Biopulping of EFB.