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Ana Belén Moldes - One of the best experts on this subject based on the ideXlab platform.
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ionic behavior assessment of surface active compounds from corn steep liquor by exchange resins
Journal of Surfactants and Detergents, 2017Co-Authors: L Rodriguezlopez, J M Cruz, X Vecino, M Rinconfontan, Ana Belén MoldesAbstract:Depending on their ionic nature, Biosurfactants can be classified as nonionic, anionic, cationic, or amphoteric. The ionic behavior of Biosurfactants is an important characteristic that dictates their use in industrial applications. In this work, a Biosurfactant extract obtained from corn steep liquor was subjected to anionic or cationic resins, in order to study the ionic behavior under different operational conditions using response surface methodology. The independent variables included in the study are the dilution of Biosurfactant solution, the amount of cationic or anionic resin, and the extraction time, whereas the dependent variables studied consisted of the surface tension of Biosurfactant aqueous solution, after contacting with anionic or cationic resin. The results showed that Biosurfactant extracted from corn steep liquor is amphoteric, since both resins were able to entrap this Biosurfactant, making it particularly suited for use in personal care preparations for sensitive skin.
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Optimization of liquid–liquid extraction of Biosurfactants from corn steep liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, Roque Devesa, Letricia Barbosa-pereira, J M Cruz, Ana Belén MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less Biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain Biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of Biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of Biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the Biosurfactant extract was 399.4 mg L^−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of Biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less Biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
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optimization of liquid liquid extraction of Biosurfactants from corn steep liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, Letricia Barbosapereira, R Devesarey, J M Cruz, Ana Belén MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less Biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain Biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of Biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of Biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the Biosurfactant extract was 399.4 mg L−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of Biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less Biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
X Vecino - One of the best experts on this subject based on the ideXlab platform.
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ionic behavior assessment of surface active compounds from corn steep liquor by exchange resins
Journal of Surfactants and Detergents, 2017Co-Authors: L Rodriguezlopez, J M Cruz, X Vecino, M Rinconfontan, Ana Belén MoldesAbstract:Depending on their ionic nature, Biosurfactants can be classified as nonionic, anionic, cationic, or amphoteric. The ionic behavior of Biosurfactants is an important characteristic that dictates their use in industrial applications. In this work, a Biosurfactant extract obtained from corn steep liquor was subjected to anionic or cationic resins, in order to study the ionic behavior under different operational conditions using response surface methodology. The independent variables included in the study are the dilution of Biosurfactant solution, the amount of cationic or anionic resin, and the extraction time, whereas the dependent variables studied consisted of the surface tension of Biosurfactant aqueous solution, after contacting with anionic or cationic resin. The results showed that Biosurfactant extracted from corn steep liquor is amphoteric, since both resins were able to entrap this Biosurfactant, making it particularly suited for use in personal care preparations for sensitive skin.
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Optimization of liquid–liquid extraction of Biosurfactants from corn steep liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, Roque Devesa, Letricia Barbosa-pereira, J M Cruz, Ana Belén MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less Biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain Biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of Biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of Biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the Biosurfactant extract was 399.4 mg L^−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of Biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less Biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
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optimization of liquid liquid extraction of Biosurfactants from corn steep liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, Letricia Barbosapereira, R Devesarey, J M Cruz, Ana Belén MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less Biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain Biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of Biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of Biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the Biosurfactant extract was 399.4 mg L−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of Biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less Biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
J M Cruz - One of the best experts on this subject based on the ideXlab platform.
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ionic behavior assessment of surface active compounds from corn steep liquor by exchange resins
Journal of Surfactants and Detergents, 2017Co-Authors: L Rodriguezlopez, J M Cruz, X Vecino, M Rinconfontan, Ana Belén MoldesAbstract:Depending on their ionic nature, Biosurfactants can be classified as nonionic, anionic, cationic, or amphoteric. The ionic behavior of Biosurfactants is an important characteristic that dictates their use in industrial applications. In this work, a Biosurfactant extract obtained from corn steep liquor was subjected to anionic or cationic resins, in order to study the ionic behavior under different operational conditions using response surface methodology. The independent variables included in the study are the dilution of Biosurfactant solution, the amount of cationic or anionic resin, and the extraction time, whereas the dependent variables studied consisted of the surface tension of Biosurfactant aqueous solution, after contacting with anionic or cationic resin. The results showed that Biosurfactant extracted from corn steep liquor is amphoteric, since both resins were able to entrap this Biosurfactant, making it particularly suited for use in personal care preparations for sensitive skin.
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Optimization of liquid–liquid extraction of Biosurfactants from corn steep liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, Roque Devesa, Letricia Barbosa-pereira, J M Cruz, Ana Belén MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less Biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain Biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of Biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of Biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the Biosurfactant extract was 399.4 mg L^−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of Biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less Biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
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optimization of liquid liquid extraction of Biosurfactants from corn steep liquor
Bioprocess and Biosystems Engineering, 2015Co-Authors: X Vecino, Letricia Barbosapereira, R Devesarey, J M Cruz, Ana Belén MoldesAbstract:In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less Biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain Biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of Biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of Biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the Biosurfactant extract was 399.4 mg L−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of Biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less Biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
Swaranjit Singh Cameotra - One of the best experts on this subject based on the ideXlab platform.
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Utilization of oleo-chemical industry by-products for Biosurfactant production
AMB Express, 2013Co-Authors: Garima Bhardwaj, Swaranjit Singh Cameotra, Harish Kumar ChopraAbstract:Biosurfactants are the surface active compounds produced by micro-organisms. The eco-friendly and biodegradable nature of Biosurfactants makes their usage more advantageous over chemical surfactants. Biosurfactants encompass the properties of dropping surface tension, stabilizing emulsions, promoting foaming and are usually non- toxic and biodegradable. Biosurfactants offer advantages over their synthetic counterparts in many applications ranging from environmental, food, and biomedical, cosmetic and pharmaceutical industries. The important environmental applications of Biosurfactants include bioremediation and dispersion of oil spills, enhanced oil recovery and transfer of crude oil. The emphasis of present review shall be with reference to the commercial production, current developments and future perspectives of a variety of approaches of Biosurfactant production from the micro-organisms isolated from various oil- contaminated sites and from the by-products of oleo-chemical industry wastes/ by-products viz. used edible oil, industrial residues, acid oil, deodorizer distillate, soap-stock etc.
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Biosurfactants in agriculture
Applied Microbiology and Biotechnology, 2013Co-Authors: Dhara P. Sachdev, Swaranjit Singh CameotraAbstract:Agricultural productivity to meet growing demands of human population is a matter of great concern for all countries. Use of green compounds to achieve the sustainable agriculture is the present necessity. This review highlights the enormous use of harsh surfactants in agricultural soil and agrochemical industries. Biosurfactants which are reported to be produced by bacteria, yeasts, and fungi can serve as green surfactants. Biosurfactants are considered to be less toxic and eco-friendly and thus several types of Biosurfactants have the potential to be commercially produced for extensive applications in pharmaceutical, cosmetics, and food industries. The Biosurfactants synthesized by environmental isolates also has promising role in the agricultural industry. Many rhizosphere and plant associated microbes produce Biosurfactant; these biomolecules play vital role in motility, signaling, and biofilm formation, indicating that Biosurfactant governs plant-microbe interaction. In agriculture, Biosurfactants can be used for plant pathogen elimination and for increasing the bioavailability of nutrient for beneficial plant associated microbes. Biosurfactants can widely be applied for improving the agricultural soil quality by soil remediation. These biomolecules can replace the harsh surfactant presently being used in million dollar pesticide industries. Thus, exploring Biosurfactants from environmental isolates for investigating their potential role in plant growth promotion and other related agricultural applications warrants details research. Conventional methods are followed for screening the microbial population for production of Biosurfactant. However, molecular methods are fewer in reaching Biosurfactants from diverse microbial population and there is need to explore novel Biosurfactant from uncultured microbes in soil biosphere by using advanced methodologies like functional metagenomics.
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Biosurfactant Production and Potential Correlation with Esterase Activity
Journal of Petroleum & Environmental Biotechnology, 2012Co-Authors: Kamaljeet Kaur Sekhon, Sunil Khanna, Swaranjit Singh CameotraAbstract:Biosurfactants (microbial surfactants) are surface active compounds produced extracellularly or as part of the cell membrane by several bacterial and fungal species. They have the unique property of reducing the surface and interfacial tension of liquids. Biosurfactants have applications in the field of agriculture, petroleum, microbial enhanced oil recovery, biomedical sciences, cosmetics, food processing and pharmaceuticals. The global Biosurfactants market has grown gradually. Regardless of their greater biodegradability and reduced toxicity, cost competitiveness still remains the major concern for Biosurfactant production. However, recombinant or metabolically engineered hyper producing strains combined with optimized cultivation conditions have made it possible for many companies to reap the benefits of ‘green’ Biosurfactant technology. Simultaneously, Biosurfactants and bioemulsifiers showing esterase activities and having potential applications are reported to form stable oil-water emulsions with hydrophobic substrates such as hexadecane and polyaromatic hydrocarbons. Biosurfactant production and release of esterases by the microbial cells is shown to be synchronized and symbiotically beneficial in some species. Several bacterial Biosurfactant and esterase genes have been identified, cloned and expressed for their enhanced production. This review article emphasizes on the present worldwide scenario of Biosurfactant production, correlation between Biosurfactant production and esterase activity, recent developments in this line of research and future prospects.
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Environmental applications of Biosurfactants: Recent advances
International Journal of Molecular Sciences, 2011Co-Authors: Magdalena Pacwa-płociniczak, Grażyna Płaza, Zofia Piotrowska-seget, Swaranjit Singh CameotraAbstract:Increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. An alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is the use of Biosurfactants and Biosurfactant-producing microorganisms. The diversity of Biosurfactants makes them an attractive group of compounds for potential use in a wide variety of industrial and biotechnological applications. The purpose of this review is to provide a comprehensive overview of advances in the applications of Biosurfactants and Biosurfactant-producing microorganisms in hydrocarbon and metal remediation technologies.
Stéphanie Rossano - One of the best experts on this subject based on the ideXlab platform.
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Biodégradation des HAPs dans les sols, assistée par lavage au Biosurfactant
2019Co-Authors: Florian Cazals, David Huguenot, Stéfan Colombano, Stéphanie Betelu, Nathalie Galopin, Arnault Perrault, Marie-odile Simonnot, Marc Crampon, Ioannis Ignatiadis, Stéphanie RossanoAbstract:Ce travail présente les résultats principaux de deux premières années d’une thèse, dont les objectifs de recherche s’articulent autour de deux axes : la production de Biosurfactants et la mise au point de traitements des pollutions par les Hydrocarbures Aromatiques Polycycliques (HAPs) par une combinaison de lavage aux Biosurfactants et de biodégradation des HAPs contenus dans des sols impactés. Pour atteindre les objectifs fixés, tout d’abord des bactéries capables de produire des Biosurfactants ont été isolées et sélectionnées à partir d’échantillons extraits d’un sol pollué. La production de Biosurfactant par ces souches bactériennes a été optimisée en sélectionnant la source de carbone la plus adaptée et en expérimentant divers rapports de concentrations entre les nutriments présents dans le milieu de culture. La purification du Biosurfactant produit a permis son analyse et son identification par Chromatographie Liquide couplée à un Spectromètre de Masse. Par ailleurs, la capacité des souches bactériennes étudiées à dégrader les HAPs a ensuite été testée en batchs. Les résultats encourageants de ces essais de biodégradation ont permis de changer d’échelle et de tester la désorption des HAPs dans des colonnes de sable et de sol par injection de Biosurfactant. La combinaison du lavage par Biosurfactant et de la biodégradation a pu être testée en colonnes pluri décimétriques, ce qui permettra de dimensionner un pilote expérimental de démonstration, qui sera réalisé à l’échelle plurimétrique, éventuellement sur site.
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Environmental remediation: Selection of a Biosurfactant for a new method of treatment for soils and waters impacted by Polycyclic Aromatic Hydrocarbons
2018Co-Authors: Florian Cazals, David Huguenot, Stéfan Colombano, Stéphanie Betelu, Nathalie Galopin, Arnault Perrault, Marie-odile Simonnot, Ioannis Ignatiadis, Stéphanie RossanoAbstract:Objectives: Former industrial sites contaminated with Polycyclic Aromatic Hydrocarbons (PAHs) are an environmental and human health concern in Europe. These sites, often former gasworks and coke factories, are close to or are nowadays part of urban areas and are persistent sources of pollution for the underground water that have to be cleaned. PAHs are also a problem for the petroleum industry. The refining of crude oil, the transport, and the petrochemical industry can release PAHs in the environment, leading to health and environment risks. Bioremediation of PAHs in pore water and soils is possible and had already been done but is limited by the low solubility of such molecules, which reduces their bioavailability. Surfactants can enhance the apparent solubility of hydrophobic compounds, like PAHs, entrapping them by the formation of micelles. Chemically-produced surfactants, widely used in soil remediation (i.e. soil washing techniques, etc.) are impaired by their low biocompatibility. Using Biosurfactants, produced by bacteria, can improve this biocompatibility and then enhance PAHs bioavailability. The objective of this project is to treat an industrial soil and the groundwater sheet associated, impacted by PAHs with Biosurfactant washing and biodegradation at the same time. The present work is part of the Bioxyval project that gathers multiple actors, from public and private sectors, of the soil remediation area. The objective of the project is to develop and implement innovative soil treatment methods, on a former industrial site, with multiple pollutions, including PAHs (former coke factory). Innovative nature of the proposed topic: The aim of the project is to develop an innovative solution for the remediation of the PAHs impacted soil by coupling a soil washing phase with Biosurfactants and a stimulated biodegradation phase of the PAHs solubilized in the water. Experimental approach: From the soil samples of the contaminated site, several bacterial cultures had been extracted and isolated. One of them shown abilities to produce Biosurfactants and had been selected for further experiments and the optimization of the Biosurfactants production. For the optimization of the Biosurfactant production, several series of analysis were made, to select the best carbon source, to find the most appropriate culture medium and nutrient balance and to see the influence of others parameters like temperature or the presence of others bacteria isolates. For the PAHs degradation assays, tests were made with soil and water samples from the contaminated site. Injections of the concentrated bacterial strain, of nutrients, or directly Biosurfactant were tested. The Biosurfactant action was compared with some commercial surfactants and Biosurfactants. Results: The Biosurfactant production tests show that the isolated bacterial culture was able to produce Biosurfactant from an insoluble or a soluble carbon source. The medium with glucose as the carbon source was the one with the fastest production and was selected for further experiment. The presence of both nitrogen and phosphorus nutrient in the culture medium seems to be mandatory for the Biosurfactant production for this bacterial isolate, an optimal balance between nitrogen, phosphorus and glucose was found to optimize the speed of the reaction. The bacterial isolate seems able to produce Biosurfactant in the presence of other bacteria. Biodegradation trials have already proved the high potential of bacteria for their ability to produce Biosurfactant, which pushes the PAHs up to micellar state. On the water samples, evidences of the use of the produced Biosurfactant for the PAHs degradation had been found. The second phase of the project is to test both the Biosurfactant production, the PAHs solubilization and the PAHs biodegradation in soil column to see the influence of the physical parameters of the soil on the remediation process. During the final step of this work, those lab trials will be extended to an in situ pilot treatment on an industrial site.