The Experts below are selected from a list of 102 Experts worldwide ranked by ideXlab platform
Andreas Vilcinskas - One of the best experts on this subject based on the ideXlab platform.
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from traditional maggot therapy to modern Biosurgery
2011Co-Authors: Andreas VilcinskasAbstract:The definition of insect biotechnology provided in this book refers to any technological application that uses insects or derivatives thereof, to make or modify products or processes for specific use in agriculture, food science, and medicine. The most prominent application of insects or insect-derived molecules in medicine is known as maggot therapy or Biosurgery. The term maggot therapy has been established for the application of live larvae from the green blow fly Lucilia sericata (Diptera: Calliphoridae) to cure open, infected, chronic and/or necrotic wounds, particularly those associated with diabetic or vascular ulcers, whose cure by conventional approaches often fails. This simple and highly successful therapy has received renewed attention in wound therapy because it combines a number of advantages such as efficacy, excellent safety record, and low costs. However, the maggots are sometimes negatively perceived by patients and their sharp mouth-hooks and spicules can sometimes cause pain. Recent research focuses on the identification of molecules from L. sericata mediating promotion of wound healing as well as debridement and disinfection of wounds. The production and application of corresponding synthetic or recombinant analogues may expand the use of insect-derived molecules beyond maggot therapy of wounds to include the cure of other diseases by Biosurgery.
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septic injury inducible genes in medicinal maggots of the green blow fly lucilia sericata
Insect Molecular Biology, 2009Co-Authors: Boran Altincicek, Andreas VilcinskasAbstract:Lucilia sericata maggots are used world-wide in Biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used the suppression subtractive hybridization method to screen for genes that are differentially expressed in response to septic wounding of sterile second instar larvae of L. sericata. This approach resulted in the identification of 65 novel Lucilia genes including potential signalling proteins (e.g. inhibitor of apoptosis 2 protein) and a number of digestive enzymes including lipases and proteinases. Additionally, we found numerous putative antimicrobial peptides (AMPs), such as a potential Lucilia defensin, diptericin and three novel proline-rich AMPs. The identified genes may facilitate access to both peptides and proteins within the beneficial excretions, secretions and haemolymph of medicinal maggots and provide novel insights into the evolution of innate immunity in Diptera.
Christine H Rohde - One of the best experts on this subject based on the ideXlab platform.
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facial reconstruction by Biosurgery cell transplantation versus cell homing
Tissue Engineering Part B-reviews, 2010Co-Authors: Michael S Stosich, Eduardo K Moioli, Susan Y Fu, Barbara Bastian, Sidney B Eisig, Candice Zemnick, Jeffrey A Ascherman, June Wu, Christine H RohdeAbstract:The face distinguishes one human being from another. When the face is disfigured because of trauma, tumor removal, congenital anomalies, or chronic diseases, the patient has a strong desire for functional and esthetic restoration. Current practice of facial reconstruction using autologous grafts, synthetic fillers, and prostheses is frequently below the surgeon's and patient's expectations. Facial reconstruction is yet to take advantage of recent advances in seemingly unrelated fields of stem cell biology, chemical engineering, biomaterials, and tissue engineering. “Biosurgery,” a new concept that we propose, will incorporate novel principles and strategies of bioactive cues, biopolymers, and/or cells to restore facial defects. Small facial defects can likely be reconstructed by cell homing and without cell transplantation. A critical advantage of cell homing is that agilely recruited endogenous cells have the potential to harness the host's innate capacity for regeneration, thus accelerating the rate of ...
A Looks - One of the best experts on this subject based on the ideXlab platform.
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Biosurgery in wound healing the renaissance of maggot therapy
Journal of The European Academy of Dermatology and Venereology, 2000Co-Authors: Uwe Wollina, Kerstin Karte, Claudia Herold, A LooksAbstract:: Chronic wounds are a challenge for modern health care. A basic principle of treatment is the removal of sloughy, necrotic, devitalized tissue to prevent wound infection and delayed healing. Biosurgery (syn. maggot or larval therapy) is a promising adjunct to the whole spectrum of topical treatment methods, in particular for debridement. The term 'Biosurgery' describes the use of living maggots on wounds to remove devitalized tissue, decrease the risk of infection and improve wound healing. The present paper gives a brief review of history, entomology, biochemistry and medical indications of Biosurgery and the practical handling of maggots. We also provide some clinical data from the literature and our own experience in a wound care unit. Biosurgery is an effective and safe treatment option for debridement and disinfection.
Michael S Stosich - One of the best experts on this subject based on the ideXlab platform.
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facial reconstruction by Biosurgery cell transplantation versus cell homing
Tissue Engineering Part B-reviews, 2010Co-Authors: Michael S Stosich, Eduardo K Moioli, Susan Y Fu, Barbara Bastian, Sidney B Eisig, Candice Zemnick, Jeffrey A Ascherman, June Wu, Christine H RohdeAbstract:The face distinguishes one human being from another. When the face is disfigured because of trauma, tumor removal, congenital anomalies, or chronic diseases, the patient has a strong desire for functional and esthetic restoration. Current practice of facial reconstruction using autologous grafts, synthetic fillers, and prostheses is frequently below the surgeon's and patient's expectations. Facial reconstruction is yet to take advantage of recent advances in seemingly unrelated fields of stem cell biology, chemical engineering, biomaterials, and tissue engineering. “Biosurgery,” a new concept that we propose, will incorporate novel principles and strategies of bioactive cues, biopolymers, and/or cells to restore facial defects. Small facial defects can likely be reconstructed by cell homing and without cell transplantation. A critical advantage of cell homing is that agilely recruited endogenous cells have the potential to harness the host's innate capacity for regeneration, thus accelerating the rate of ...
Uwe Wollina - One of the best experts on this subject based on the ideXlab platform.
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Biosurgery supports granulation and debridement in chronic wounds clinical data and remittance spectroscopy measurement
International Journal of Dermatology, 2002Co-Authors: Uwe Wollina, Kristin Liebold, Wolfdieter Schmidt, Michael Hartmann, Dieter FasslerAbstract:Background Maggot therapy (Biosurgery) has received increasing interest for the debridement of chronic wounds and for the improvement of wound healing. The purpose of this study was to investigate the clinical effects, side-effects, and possible mechanisms of action of Biosurgery. Methods Biosurgery was used for debridement in 30 patients with chronic leg ulcers of mixed origin. The effect of a single application of maggots for 1–4 days was evaluated by a clinical wound score and contact-free spectroscopy. Side-effects were recorded. Results Debridement was rapid and selective. The wound secretion was temporarily increased. We observed a significant improvement of the wound score with a decrease from 13.5 ± 1.8 to 6.3 ± 2.7 (P < 0.001). The treatment was well tolerated in most patients. Twelve out of 30 patients reported temporary pain, but only two needed analgesic treatment. Other side-effects included venous bleeding in one patient. The remittance spectra showed an improvement of tissue oxygenation as revealed by the characteristic oxygen doublet peak (548 and 575 nm). Conclusions Biosurgery is an effective and rapid treatment for the debridement of chronic wounds and the improvement of wound healing. A possible mode of action is the increase in tissue oxygenation. More studies are needed.
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Biosurgery in wound healing the renaissance of maggot therapy
Journal of The European Academy of Dermatology and Venereology, 2000Co-Authors: Uwe Wollina, Kerstin Karte, Claudia Herold, A LooksAbstract:: Chronic wounds are a challenge for modern health care. A basic principle of treatment is the removal of sloughy, necrotic, devitalized tissue to prevent wound infection and delayed healing. Biosurgery (syn. maggot or larval therapy) is a promising adjunct to the whole spectrum of topical treatment methods, in particular for debridement. The term 'Biosurgery' describes the use of living maggots on wounds to remove devitalized tissue, decrease the risk of infection and improve wound healing. The present paper gives a brief review of history, entomology, biochemistry and medical indications of Biosurgery and the practical handling of maggots. We also provide some clinical data from the literature and our own experience in a wound care unit. Biosurgery is an effective and safe treatment option for debridement and disinfection.