The Experts below are selected from a list of 3372 Experts worldwide ranked by ideXlab platform

Ziyin Yang - One of the best experts on this subject based on the ideXlab platform.

  • study of the biochemical formation pathway of aroma compound 1 phenylethanol in tea camellia sinensis l o kuntze flowers and other plants
    Food Chemistry, 2018
    Co-Authors: Ying Zhou, Lanting Zeng, Fang Dong, Qiyuan Peng, Jinchi Tang, Ziyin Yang
    Abstract:

    After tea leaves, tea (Camellia sinensis) flowers are becoming a second tea plant resource because they contain not only functional metabolites similar to those found in tea leaves, but also predominant amounts of functional metabolites that only occur in tea leaves in small amounts. 1-Phenylethanol (1PE) is a predominant aroma compound found in tea flowers. A 1PE synthase in tea flowers was isolated, functionally characterized, and shown to have the highest catalytic efficiency for the conversion of acetophenone (AP). To determine why 1PE accumulates more in tea flowers than other plants, we compared their 1PE contents and used a stable isotope labeling method to elucidate the 1PE Biosynthetic Route. Supplementation with [2H8]l-phenylalanine and [2H5]AP suggested that most plants containing the enzyme/gene catalyzed the conversion of AP to 1PE. Furthermore, the availability of AP derived from l-phenylalanine was responsible for the difference in 1PE accumulation between tea flowers and other plants.

  • studies on the biochemical formation pathway of the amino acid l theanine in tea camellia sinensis and other plants
    Journal of Agricultural and Food Chemistry, 2017
    Co-Authors: Sihua Cheng, Yinyin Liao, Lanting Zeng, Xiaoqin Wang, Fang Dong, Ziyin Yang
    Abstract:

    Tea (Camellia sinensis) is the most widely consumed beverage aside from water. The flavor of tea is conferred by certain metabolites, especially l-theanine, in C. sinensis. To determine why more l-theanine accumulates in C. sinensis than in other plants, we compare l-theanine contents between C. sinensis and other plant species (Camellia nitidissima, Camellia japonica, Zea mays, Arabidopsis thaliana, and Solanum lycopersicum) and use a stable isotope labeling approach to elucidate its Biosynthetic Route. We quantify relevant intermediates and metabolites by mass spectrometry. l-Glutamic acid, a precursor of l-theanine, is present in most plants, while ethylamine, another precursor of l-theanine, specifically accumulates in Camellia species, especially C. sinensis. Most plants contain the enzyme/gene catalyzing the conversion of ethylamine and l-glutamic acid to l-theanine. After supplementation with [2H5]ethylamine, all the plants produce [2H5]l-theanine, which suggests that ethylamine availability is the...

Rolf Müller - One of the best experts on this subject based on the ideXlab platform.

  • High-titer heterologous production in E. coli of lyngbyatoxin, a protein kinase C activator from an uncultured marine cyanobacterium.
    ACS Chemical Biology, 2013
    Co-Authors: Sarah E. Ongley, Xiaoying Bian, Rocky Chau, William H Gerwick, Youming Zhang, Rolf Müller
    Abstract:

    Many chemically complex cyanobacterial polyketides and nonribosomal peptides are of great pharmaceutical interest, but the levels required for exploitation are difficult to achieve from native sources. Here we develop a framework for the expression of these multifunctional cyanobacterial assembly lines in Escherichia coli using the lyngbyatoxin Biosynthetic pathway, derived from a marine microbial assemblage dominated by the cyanobacterium Moorea producens. Heterologous expression of this pathway afforded high titers of both lyngbyatoxin A (25.6 mg L–1) and its precursor indolactam-V (150 mg L–1). Production, isolation, and identification of all expected chemical intermediates of lyngbyatoxin biosynthesis in E. coli also confirmed the previously proposed Biosynthetic Route, setting a solid chemical foundation for future pathway engineering. The successful production of the nonribosomal peptide lyngbyatoxin A in E. coli also opens the possibility for future heterologous expression, characterization, and ex...

Sarah E. Ongley - One of the best experts on this subject based on the ideXlab platform.

  • High-Titer Heterologous Production in E. coli of Lyngbyatoxin, a Protein Kinase C Activator from an Uncultured Marine Cyanobacterium
    2016
    Co-Authors: Sarah E. Ongley, Xiaoying Bian, Rocky Chau, William H Gerwick, Youming Zhang, Rolf Müller
    Abstract:

    Many chemically complex cyanobacterial polyketides and nonribosomal peptides are of great pharmaceutical interest, but the levels required for exploitation are difficult to achieve from native sources. Here we develop a framework for the expression of these multifunctional cyanobacterial assembly lines in Escherichia coli using the lyngbyatoxin Biosynthetic pathway, derived from a marine microbial assemblage dominated by the cyanobacterium Moorea producens. Heterologous expression of this pathway afforded high titers of both lyngbyatoxin A (25.6 mg L–1) and its precursor indolactam-V (150 mg L–1). Production, isolation, and identification of all expected chemical intermediates of lyngbyatoxin biosynthesis in E. coli also confirmed the previously proposed Biosynthetic Route, setting a solid chemical foundation for future pathway engineering. The successful production of the nonribosomal peptide lyngbyatoxin A in E. coli also opens the possibility for future heterologous expression, characterization, and exploitation of other cyanobacterial natural product pathways

  • High-titer heterologous production in E. coli of lyngbyatoxin, a protein kinase C activator from an uncultured marine cyanobacterium.
    ACS Chemical Biology, 2013
    Co-Authors: Sarah E. Ongley, Xiaoying Bian, Rocky Chau, William H Gerwick, Youming Zhang, Rolf Müller
    Abstract:

    Many chemically complex cyanobacterial polyketides and nonribosomal peptides are of great pharmaceutical interest, but the levels required for exploitation are difficult to achieve from native sources. Here we develop a framework for the expression of these multifunctional cyanobacterial assembly lines in Escherichia coli using the lyngbyatoxin Biosynthetic pathway, derived from a marine microbial assemblage dominated by the cyanobacterium Moorea producens. Heterologous expression of this pathway afforded high titers of both lyngbyatoxin A (25.6 mg L–1) and its precursor indolactam-V (150 mg L–1). Production, isolation, and identification of all expected chemical intermediates of lyngbyatoxin biosynthesis in E. coli also confirmed the previously proposed Biosynthetic Route, setting a solid chemical foundation for future pathway engineering. The successful production of the nonribosomal peptide lyngbyatoxin A in E. coli also opens the possibility for future heterologous expression, characterization, and ex...

Lanting Zeng - One of the best experts on this subject based on the ideXlab platform.

  • study of the biochemical formation pathway of aroma compound 1 phenylethanol in tea camellia sinensis l o kuntze flowers and other plants
    Food Chemistry, 2018
    Co-Authors: Ying Zhou, Lanting Zeng, Fang Dong, Qiyuan Peng, Jinchi Tang, Ziyin Yang
    Abstract:

    After tea leaves, tea (Camellia sinensis) flowers are becoming a second tea plant resource because they contain not only functional metabolites similar to those found in tea leaves, but also predominant amounts of functional metabolites that only occur in tea leaves in small amounts. 1-Phenylethanol (1PE) is a predominant aroma compound found in tea flowers. A 1PE synthase in tea flowers was isolated, functionally characterized, and shown to have the highest catalytic efficiency for the conversion of acetophenone (AP). To determine why 1PE accumulates more in tea flowers than other plants, we compared their 1PE contents and used a stable isotope labeling method to elucidate the 1PE Biosynthetic Route. Supplementation with [2H8]l-phenylalanine and [2H5]AP suggested that most plants containing the enzyme/gene catalyzed the conversion of AP to 1PE. Furthermore, the availability of AP derived from l-phenylalanine was responsible for the difference in 1PE accumulation between tea flowers and other plants.

  • studies on the biochemical formation pathway of the amino acid l theanine in tea camellia sinensis and other plants
    Journal of Agricultural and Food Chemistry, 2017
    Co-Authors: Sihua Cheng, Yinyin Liao, Lanting Zeng, Xiaoqin Wang, Fang Dong, Ziyin Yang
    Abstract:

    Tea (Camellia sinensis) is the most widely consumed beverage aside from water. The flavor of tea is conferred by certain metabolites, especially l-theanine, in C. sinensis. To determine why more l-theanine accumulates in C. sinensis than in other plants, we compare l-theanine contents between C. sinensis and other plant species (Camellia nitidissima, Camellia japonica, Zea mays, Arabidopsis thaliana, and Solanum lycopersicum) and use a stable isotope labeling approach to elucidate its Biosynthetic Route. We quantify relevant intermediates and metabolites by mass spectrometry. l-Glutamic acid, a precursor of l-theanine, is present in most plants, while ethylamine, another precursor of l-theanine, specifically accumulates in Camellia species, especially C. sinensis. Most plants contain the enzyme/gene catalyzing the conversion of ethylamine and l-glutamic acid to l-theanine. After supplementation with [2H5]ethylamine, all the plants produce [2H5]l-theanine, which suggests that ethylamine availability is the...

Fang Dong - One of the best experts on this subject based on the ideXlab platform.

  • study of the biochemical formation pathway of aroma compound 1 phenylethanol in tea camellia sinensis l o kuntze flowers and other plants
    Food Chemistry, 2018
    Co-Authors: Ying Zhou, Lanting Zeng, Fang Dong, Qiyuan Peng, Jinchi Tang, Ziyin Yang
    Abstract:

    After tea leaves, tea (Camellia sinensis) flowers are becoming a second tea plant resource because they contain not only functional metabolites similar to those found in tea leaves, but also predominant amounts of functional metabolites that only occur in tea leaves in small amounts. 1-Phenylethanol (1PE) is a predominant aroma compound found in tea flowers. A 1PE synthase in tea flowers was isolated, functionally characterized, and shown to have the highest catalytic efficiency for the conversion of acetophenone (AP). To determine why 1PE accumulates more in tea flowers than other plants, we compared their 1PE contents and used a stable isotope labeling method to elucidate the 1PE Biosynthetic Route. Supplementation with [2H8]l-phenylalanine and [2H5]AP suggested that most plants containing the enzyme/gene catalyzed the conversion of AP to 1PE. Furthermore, the availability of AP derived from l-phenylalanine was responsible for the difference in 1PE accumulation between tea flowers and other plants.

  • studies on the biochemical formation pathway of the amino acid l theanine in tea camellia sinensis and other plants
    Journal of Agricultural and Food Chemistry, 2017
    Co-Authors: Sihua Cheng, Yinyin Liao, Lanting Zeng, Xiaoqin Wang, Fang Dong, Ziyin Yang
    Abstract:

    Tea (Camellia sinensis) is the most widely consumed beverage aside from water. The flavor of tea is conferred by certain metabolites, especially l-theanine, in C. sinensis. To determine why more l-theanine accumulates in C. sinensis than in other plants, we compare l-theanine contents between C. sinensis and other plant species (Camellia nitidissima, Camellia japonica, Zea mays, Arabidopsis thaliana, and Solanum lycopersicum) and use a stable isotope labeling approach to elucidate its Biosynthetic Route. We quantify relevant intermediates and metabolites by mass spectrometry. l-Glutamic acid, a precursor of l-theanine, is present in most plants, while ethylamine, another precursor of l-theanine, specifically accumulates in Camellia species, especially C. sinensis. Most plants contain the enzyme/gene catalyzing the conversion of ethylamine and l-glutamic acid to l-theanine. After supplementation with [2H5]ethylamine, all the plants produce [2H5]l-theanine, which suggests that ethylamine availability is the...