The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Ting Liu - One of the best experts on this subject based on the ideXlab platform.
-
the plant Alkaloid tetrandrine inhibits metastasis via autophagy dependent wnt β catenin and metastatic tumor antigen 1 signaling in human liver cancer cells
Journal of Experimental & Clinical Cancer Research, 2018Co-Authors: Zhenxing Zhang, Ting LiuAbstract:Tetrandrine is a Bisbenzylisoquinoline Alkaloid isolated from the Chinese medicinal herb Stephania tetrandra S. Moore. We previously demonstrated that tetrandrine exhibits potent antitumor effects in many types of cancer cells. In this study, we investigated the effects of tetrandrine on human hepatocellular carcinoma (HCC) metastasis. The invasion and migration effects were evaluated via wound healing and transwell assays. Immunofluorescence and western blotting analyses were used to investigate the levels of epithelial-mesenchymal transition (EMT)-related protein. A metastasis model was established to investigate the inhibitory effect of tetrandrine on hepatocellular carcinoma metastasis in vivo. Tetrandrine inhibits HCC invasion and migration by preventing cell EMT. The underlying mechanism was closely associated with tetrandrine-induced human liver cell autophagy, which inhibits Wnt/β-catenin pathway activity and decreases metastatic tumor antigen 1 (MTA1) expression to modulate cancer cell metastasis. Our findings demonstrate, for the first time, that tetrandrine plays a significant role in the inhibition of human hepatocellular carcinoma metastasis and provide novel insights into the application of tetrandrine in clinical HCC treatment.
-
c-MYC and reactive oxygen species play roles in tetrandrine-induced leukemia differentiation
Nature Publishing Group, 2018Co-Authors: Ting LiuAbstract:Abstract Tetrandrine is a broadly used Bisbenzylisoquinoline Alkaloid component of traditional Chinese medicine that has antitumor effects in some cancer types. In this study, we investigated the effects of tetrandrine on leukemia in vitro and in vivo. The results showed that tetrandrine effectively induced differentiation and autophagy in leukemia cells. In addition, tetrandrine treatment activated the accumulation of reactive oxygen species (ROS) and inhibited c-MYC protein expression. Further, we found that treatment with the ROS scavengers N-acetyl-L-cysteine (NAC) and Tiron as well as overexpression of c-MYC reduced tetrandrine-induced autophagy and differentiation. Moreover, a small molecular c-MYC inhibitor, 10058-F4, enhanced the tetrandrine-induced differentiation of leukemia cells. These results suggest that ROS generation and c-MYC suppression play important roles in tetrandrine-induced autophagy and differentiation, and the results from in vivo experiments were consistent with those from in vitro studies. Therefore, our data suggest that tetrandrine may be a promising agent for the treatment of leukemia
-
The plant Alkaloid tetrandrine inhibits metastasis via autophagy-dependent Wnt/β-catenin and metastatic tumor antigen 1 signaling in human liver cancer cells
BMC, 2018Co-Authors: Zhenxing Zhang, Ting LiuAbstract:Abstract Background Tetrandrine is a Bisbenzylisoquinoline Alkaloid isolated from the Chinese medicinal herb Stephania tetrandra S. Moore. We previously demonstrated that tetrandrine exhibits potent antitumor effects in many types of cancer cells. In this study, we investigated the effects of tetrandrine on human hepatocellular carcinoma (HCC) metastasis. Methods The invasion and migration effects were evaluated via wound healing and transwell assays. Immunofluorescence and western blotting analyses were used to investigate the levels of epithelial-mesenchymal transition (EMT)-related protein. A metastasis model was established to investigate the inhibitory effect of tetrandrine on hepatocellular carcinoma metastasis in vivo. Results Tetrandrine inhibits HCC invasion and migration by preventing cell EMT. The underlying mechanism was closely associated with tetrandrine-induced human liver cell autophagy, which inhibits Wnt/β-catenin pathway activity and decreases metastatic tumor antigen 1 (MTA1) expression to modulate cancer cell metastasis. Conclusion Our findings demonstrate, for the first time, that tetrandrine plays a significant role in the inhibition of human hepatocellular carcinoma metastasis and provide novel insights into the application of tetrandrine in clinical HCC treatment
-
tetrandrine is a potent cell autophagy agonist via activated intracellular reactive oxygen species
Cell & Bioscience, 2015Co-Authors: Haiqing Wang, Ting Liu, Qin Wang, Xin LiuAbstract:Background Autophagy is an evolutionarily conserved cellular process that involves the lysosomal degradation of proteins and organelles and the recycling of cellular components to ensure cellular survival under external or internal stress. Numerous data has indicated that autophagy can be successfully targeted for the treatment of multiple cancers. We have previously demonstrated that tetrandrine, a Bisbenzylisoquinoline Alkaloid isolated from the broadly used Chinese medicinal herb Stephaniae tetrandrae, exhibits potent antitumor effects when used either alone or in combination with other drugs.
-
tetrandrine induces g1 s cell cycle arrest through the ros akt pathway in eoma cells and inhibits angiogenesis in vivo
International Journal of Oncology, 2015Co-Authors: Wenkai Xiao, Zebo Huang, Yajie Jiang, Qiuxu Men, Ling Yuan, Ting Liu, Xin LiuAbstract:Tetrandrine, a Bisbenzylisoquinoline Alkaloid, is known to inhibit tumor cell proliferation and induce apoptosis in cancer models in vitro and in vivo. In the present study, tetrandrine significantly inhibited the proliferation of mouse endothelial cells (EOMA cell) and induced G1/S arrest in EOMA cells, in which the expressions of cyclin D and cyclin E and CDKs were downregulated. Tetrandrine treatment also caused intracellular accumulation of reactive oxygen species (ROS). Pretreatment with NAC, which is a ROS inhibitor, blocked G1/S cell arrest and cyclin regulation induced by tetrandrine, implying that ROS generation plays an important role in tetrandrine-induced cell cycle arrest. Furthermore, a decreased phospho-Akt protein level after tetrandrine treatment was reversible with the removal of the intracellular ROS by NAC. Notably, overexpression of Akt decreased tetrandrine-induced G1/S arrest. Finally, we verified the antiangiogenic effects of tetrandrine in vivo in a liver cancer xenograft model in nude mice. In conclusion, tetrandrine inhibits EOMA cell growth through the ROS/Akt pathway, and it could be a promising compound for cancer therapy as an inhibitor of tumor vascular growth.
J Barbosa M Filho - One of the best experts on this subject based on the ideXlab platform.
-
modification of ca2 metabolism in the rabbit aorta as a mechanism of spasmolytic action of warifteine a Bisbenzylisoquinoline Alkaloid isolated from the leaves of cissampelos sympodialis eichl menispermaceae
Journal of Pharmacy and Pharmacology, 2011Co-Authors: M R De Freitas, G Thomas, S De F Cortes, J Barbosa M FilhoAbstract:The regulation of intracellular Ca 2+ as a mechanism of spasmolytic activity of a Bisbenzylisoquinoline Alkaloid, warifteine, isolated from the leaves of Cissampelos sympodialis, Eichl (Menispermaceae) was studied in the rabbit aorta. Warifteine (pD' 2 4.12 ± 0.09) similar to verapamil (pD' 2 6.89 ± 0.05) antagonized, in a noncompetitive and reversible manner, KCl-induced contractions, mediated by Ca 2+ entry through voltage-operated channels. Noradrenaline-induced sustained contractions mediated by Ca 2+ entry through receptor-operated channels were also inhibited by warifteine (IC50 6.03 x 10 -5 M) and the standard agent sodium nitroprusside (IC50 1.9 x 10 -8 M). In Ca 2+ -free medium, the Alkaloid reduced the intracellular Ca 2+ -dependent transient contraction to noradrenaline by inhibiting the release of Ca 2+ (IC50 2.6 x 10 -5 M) from the stores and the refilling (IC50 1.9 x 10 -5 M) of the intracellular stores. The standard agent, procaine, also inhibited the release of Ca 2+ (IC50 3.2 x 10 -5 M) but had no significant effect on Ca 2+ uptake into the stores. Warifteine failed to affect intracellular Ca 2+ stores sensitive to caffeine, while procaine inhibited (IC50 7.9 x 10 -4 M) the release of Ca 2+ from these stores. The results indicate that warifteine may cause muscle relaxation by inhibiting Ca 2+ channels and by modifying the intracellular Ca 2+ stores sensitive to noradrenaline.
-
warifteine a Bisbenzylisoquinoline Alkaloid decreases immediate allergic and thermal hyperalgesic reactions in sensitized animals
International Immunopharmacology, 2008Co-Authors: Hermann Ferreira Costa, J Barbosa M Filho, Claudio Roberto Bezerrasantos, Marco A Martins, Márcia Regina PiuvezamAbstract:Warifteine is a Bisbenzylisoquinoline Alkaloid isolated from the Cissampelos sympodialis Eichl (Menispermaceae). This plant is used in the folk medicine for the treatment of airway respiratory diseases. A murine model of immediate allergic reaction was used to evaluate warifteine treatment in the IgE production, leukocyte activation, thermal hyperalgesia, mast cell degranulation and scratching behavior. BALB/c mice treated with warifteine (0.4-10 mg/Kg) 1 h before OVA sensitization reduced OVA induced paw edema as well as the OVA-specific IgE serum titers as compared with non-treated and OVA-sensitized animals. Warifteine also reduced the mice death evoked by IgE-dependent anaphylactic shock reaction at 30 min after intravenous OVA challenge. To assess the effect of warifteine treatment on T cell proliferative response, spleen cells from warifteine treated or non-treated and OVA-sensitized animals were evaluated. Spleen cells from warifteine treated animals (2.0 mg/kg) did not proliferate following OVA stimulation as compared with spleen cell cultures from non-treated animals. This response may be related with the increase of NO production as observed in peritoneal macrophage cultures treated with warifteine. Thermal hyperalgesia evoked by IgE or histamine/5-hydroxytryptamine challenge was inhibited on rats at dose of 4.0 mg/kg. Warifteine treatment (0.6 or 6.0 microg/ml) also decreased the IgEalphaDNP-BSA sensitized mast degranulation after DNP-BSA challenge measured by histamine release. In addition, compound 48/80-induced scratching behavior was also sensitive to warifteine treatment. These results demonstrate for the first time that warifteine treatment reduced the allergy-associated responses.
-
spasmolytic actions of warifteine a Bisbenzylisoquinoline Alkaloid isolated from the root bark of cissampelos sympodialis eichl menispermaceae
Phytotherapy Research, 1995Co-Authors: D F Cortes, J L De Alencar, G Thomas, J Barbosa M FilhoAbstract:Warifteine, a Bisbenzylisoquinoline Alkaloid isolated from the root bark of Cissampelos sympodialis Eichl., produced a reversible, nonspecific and noncompetitive antagonism of histamine, carbachol and bradykinin induced contractions of the guinea-pig ileum. The corresponding pD' 2 values (mean±SE) were 4.90±0.15, 4.95±0.20 and 5.03±0.11. Warifteine also antagonized oxytocin and bradykinin induced contractions of the rat uterus in a similar manner with pD' 2 values of 4.30±0.26 and 3.76±0.06 respectively. In the guinea-pig trachea, the Alkaloid inhibited spontaneous tone (IC 50 , 1.1X10 -5 M) as well as carbachol induced sustained contractions (IC 50 , 2.9x10 -5 M). As warifteine antagonized KCI induced contractions of the guinea-pig ileum (pD' 2 value 4.57 ± 0.10), inhibition of Ca ++ influx through voltage operated Ca ++ channels may be partially responsible for its antispasmodic activity. However, the reported local anaesthetic property of warifteine may not contribute to the observed muscle relaxation as procaine failed to reduce the spontaneous tone or consistently antagonize carbachol induced contractions of the trachea and was inactive in inhibiting voltage operated Ca ++ channels in the ileum.
Xin Liu - One of the best experts on this subject based on the ideXlab platform.
-
synergistic anti tumour effects of tetrandrine and chloroquine combination therapy in human cancer a potential antagonistic role for p21
British Journal of Pharmacology, 2015Co-Authors: Liufeng Mei, Yicheng Chen, Zhimeng Wang, Jian Wang, Jiali Wan, Xin LiuAbstract:Background and Purpose Tetrandrine, a Bisbenzylisoquinoline Alkaloid isolated from the Chinese medicinal herb Stephaniae tetrandrae, has a long history in Chinese clinical applications to treat diverse diseases. Tetrandrine induced apoptosis or, at low concentrations, autophagy of human hepatocellular carcinoma cells. Here we have tested the effects of inhibitors of autophagy such as chloroquine, on the response to low concentrations of tetrandrine in cancer cells.
-
tetrandrine is a potent cell autophagy agonist via activated intracellular reactive oxygen species
Cell & Bioscience, 2015Co-Authors: Haiqing Wang, Ting Liu, Qin Wang, Xin LiuAbstract:Background Autophagy is an evolutionarily conserved cellular process that involves the lysosomal degradation of proteins and organelles and the recycling of cellular components to ensure cellular survival under external or internal stress. Numerous data has indicated that autophagy can be successfully targeted for the treatment of multiple cancers. We have previously demonstrated that tetrandrine, a Bisbenzylisoquinoline Alkaloid isolated from the broadly used Chinese medicinal herb Stephaniae tetrandrae, exhibits potent antitumor effects when used either alone or in combination with other drugs.
-
tetrandrine induces g1 s cell cycle arrest through the ros akt pathway in eoma cells and inhibits angiogenesis in vivo
International Journal of Oncology, 2015Co-Authors: Wenkai Xiao, Zebo Huang, Yajie Jiang, Qiuxu Men, Ling Yuan, Ting Liu, Xin LiuAbstract:Tetrandrine, a Bisbenzylisoquinoline Alkaloid, is known to inhibit tumor cell proliferation and induce apoptosis in cancer models in vitro and in vivo. In the present study, tetrandrine significantly inhibited the proliferation of mouse endothelial cells (EOMA cell) and induced G1/S arrest in EOMA cells, in which the expressions of cyclin D and cyclin E and CDKs were downregulated. Tetrandrine treatment also caused intracellular accumulation of reactive oxygen species (ROS). Pretreatment with NAC, which is a ROS inhibitor, blocked G1/S cell arrest and cyclin regulation induced by tetrandrine, implying that ROS generation plays an important role in tetrandrine-induced cell cycle arrest. Furthermore, a decreased phospho-Akt protein level after tetrandrine treatment was reversible with the removal of the intracellular ROS by NAC. Notably, overexpression of Akt decreased tetrandrine-induced G1/S arrest. Finally, we verified the antiangiogenic effects of tetrandrine in vivo in a liver cancer xenograft model in nude mice. In conclusion, tetrandrine inhibits EOMA cell growth through the ROS/Akt pathway, and it could be a promising compound for cancer therapy as an inhibitor of tumor vascular growth.
Xiuping Chen - One of the best experts on this subject based on the ideXlab platform.
-
neferine a Bisbenzylisoquinoline Alkaloid ameliorates dextran sulfate sodium induced ulcerative colitis
The American Journal of Chinese Medicine, 2018Co-Authors: Yanling Guo, Xiangjing Min, Lixia Pei, Xiuping ChenAbstract:Both the incidence and prevalence of ulcerative colitis (UC) are increasing throughout the world. Neferine, a natural Alkaloid, demonstrated a variety of biological activities. In this study, the a...
G Thomas - One of the best experts on this subject based on the ideXlab platform.
-
modification of ca2 metabolism in the rabbit aorta as a mechanism of spasmolytic action of warifteine a Bisbenzylisoquinoline Alkaloid isolated from the leaves of cissampelos sympodialis eichl menispermaceae
Journal of Pharmacy and Pharmacology, 2011Co-Authors: M R De Freitas, G Thomas, S De F Cortes, J Barbosa M FilhoAbstract:The regulation of intracellular Ca 2+ as a mechanism of spasmolytic activity of a Bisbenzylisoquinoline Alkaloid, warifteine, isolated from the leaves of Cissampelos sympodialis, Eichl (Menispermaceae) was studied in the rabbit aorta. Warifteine (pD' 2 4.12 ± 0.09) similar to verapamil (pD' 2 6.89 ± 0.05) antagonized, in a noncompetitive and reversible manner, KCl-induced contractions, mediated by Ca 2+ entry through voltage-operated channels. Noradrenaline-induced sustained contractions mediated by Ca 2+ entry through receptor-operated channels were also inhibited by warifteine (IC50 6.03 x 10 -5 M) and the standard agent sodium nitroprusside (IC50 1.9 x 10 -8 M). In Ca 2+ -free medium, the Alkaloid reduced the intracellular Ca 2+ -dependent transient contraction to noradrenaline by inhibiting the release of Ca 2+ (IC50 2.6 x 10 -5 M) from the stores and the refilling (IC50 1.9 x 10 -5 M) of the intracellular stores. The standard agent, procaine, also inhibited the release of Ca 2+ (IC50 3.2 x 10 -5 M) but had no significant effect on Ca 2+ uptake into the stores. Warifteine failed to affect intracellular Ca 2+ stores sensitive to caffeine, while procaine inhibited (IC50 7.9 x 10 -4 M) the release of Ca 2+ from these stores. The results indicate that warifteine may cause muscle relaxation by inhibiting Ca 2+ channels and by modifying the intracellular Ca 2+ stores sensitive to noradrenaline.
-
spasmolytic actions of warifteine a Bisbenzylisoquinoline Alkaloid isolated from the root bark of cissampelos sympodialis eichl menispermaceae
Phytotherapy Research, 1995Co-Authors: D F Cortes, J L De Alencar, G Thomas, J Barbosa M FilhoAbstract:Warifteine, a Bisbenzylisoquinoline Alkaloid isolated from the root bark of Cissampelos sympodialis Eichl., produced a reversible, nonspecific and noncompetitive antagonism of histamine, carbachol and bradykinin induced contractions of the guinea-pig ileum. The corresponding pD' 2 values (mean±SE) were 4.90±0.15, 4.95±0.20 and 5.03±0.11. Warifteine also antagonized oxytocin and bradykinin induced contractions of the rat uterus in a similar manner with pD' 2 values of 4.30±0.26 and 3.76±0.06 respectively. In the guinea-pig trachea, the Alkaloid inhibited spontaneous tone (IC 50 , 1.1X10 -5 M) as well as carbachol induced sustained contractions (IC 50 , 2.9x10 -5 M). As warifteine antagonized KCI induced contractions of the guinea-pig ileum (pD' 2 value 4.57 ± 0.10), inhibition of Ca ++ influx through voltage operated Ca ++ channels may be partially responsible for its antispasmodic activity. However, the reported local anaesthetic property of warifteine may not contribute to the observed muscle relaxation as procaine failed to reduce the spontaneous tone or consistently antagonize carbachol induced contractions of the trachea and was inactive in inhibiting voltage operated Ca ++ channels in the ileum.