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Bivariate Analysis

The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform

Andrew Milgate – 1st expert on this subject based on the ideXlab platform

  • Bivariate Analysis of barley scald resistance with relative maturity reveals a new major qtl on chromosome 3h
    Scientific Reports, 2019
    Co-Authors: Xuechen Zhang, Ben Ovenden, Beverley A Orchard, Meixue Zhou, R F Park, Davinder Singh, Andrew Milgate

    Abstract:

    The disease scald of barley is caused by the pathogen Rhynchosporium commune and can cause up to 30–40% yield loss in susceptible cultivars. In this study, the Australian barley cultivar ‘Yerong’ was demonstrated to have resistance that differed from Turk (Rrs1 (Rh3 type)) based on seedling tests with 11 R. commune isolates. A doubled haploid population with 177 lines derived from a cross between ‘Yerong’ and the susceptible Australian cultivar ‘Franklin’ was used to identify quantitative trait loci (QTL) for scald resistance. A QTL on chromosome 3H was identified with large effect, consistent with a major gene conferring scald resistance at the seedling stage. Under field conditions, a Bivariate Analysis was used to model scald percentage of infected leaf area and relative maturity, the residuals from the regression were used as our phenotype for QTL Analysis. This Analysis identified one major QTL on chromosome 3H, which mapped to the same position as the QTL at seedling stage. The identified QTL on 3H is proposed to be different from the Rrs1 on the basis of seedling resistance against different R. commune isolates and physical map position. This study increases the current understanding of scald resistance and identifies genetic material possessing QTLs useful for the marker-assisted selection of scald resistance in barley breeding programs.

  • Bivariate Analysis of barley scald resistance with relative maturity reveals a new major qtl on chromosome 3h
    bioRxiv, 2019
    Co-Authors: Xuechen Zhang, Ben Ovenden, Beverley A Orchard, Meixue Zhou, R F Park, Davinder Singh, Andrew Milgate

    Abstract:

    Abstract The disease scald of barley is caused by the pathogen Rhynchosporium commune and can cause up to 30-40% yield loss in susceptible varieties. In this study, the Australian barley cultivar Yerong was demonstrated to have resistance that differed from Turk (Rrs1) based on seedling tests with 11 R. commune isolates. A doubled haploid population with 177 lines derived from a cross between Yerong and Franklin was used to identify quantitative trait loci (QTL) for scald resistance. Scald resistance against four pathogen isolates was assessed at the seedling growth stage in a glasshouse experiment and at the adult growth stage in field experiments with natural infection over three consecutive years. A QTL on chromosome 3H was identified with large effect, consistent with a major gene conferring scald resistance at the seedling stage. Under field conditions, scald percentage was negatively correlated with early relative maturity. A Bivariate Analysis was used to model scald percentage and relative maturity together, residuals from the regression of scald percentage on relative maturity were used as our phenotype for QTL Analysis. This Analysis identified one major QTL on chromosome 3H, which mapped to the same position as the QTL identified for scald resistance at seedling stage. The identified QTL on 3H is proposed to be different from the Rrs1 on the basis of seedling resistance against different R. commune isolates and physical map position. The Analysis also identified an additional novel QTL on chromosome 7H. This study increases the current understanding of scald resistance and identifies genetic material possessing QTLs useful for the marker-assisted selection of scald resistance in barley breeding programs.

Zbigniew Darzynkiewicz – 2nd expert on this subject based on the ideXlab platform

  • Bivariate Analysis of cellular DNA versus RNA content by laser scanning cytometry using the product of signal subtraction (differential fluorescence) as a separate parameter.
    Cytometry, 2001
    Co-Authors: Piotr Smolewski, Jerzy Grabarek, Louis A. Kamentsky, Zbigniew Darzynkiewicz

    Abstract:

    Background

    The cytometric methods of Bivariate Analysis of cellular RNA versus DNA content have limitations. The method based on the use of metachromatic fluorochrome acridine orange (AO) requires rigorous conditions of the equilibrium staining whereas pyronin Y and Hoechst 33342 necessitate the use of an instrument that provides two-laser excitation, including the ultraviolet (UV) light wavelength.

    Methods

    Phytohemagglutinin (PHA)-stimulated human lymphocytes were deposited on microscope slides and fixed. DNA and double-stranded (ds) RNA were stained with propidium iodide (PI) and protein was stained with BODIPY 630/650-X or fluorescein isothiocyanate (FITC). Cellular fluorescence was measured with a laser scanning cytometer (LSC). The cells were treated with RNase A and their fluorescence was measured again. The file-merge feature of the LSC was used to record the cell PI fluorescence measurements prior to and after the RNase treatment in list mode, as a single file. The integrated PI fluorescence intensity of each cell after RNase treatment was subtracted from the fluorescence intensity of the same cell measured prior to RNase treatment. This RNase-specific differential value of fluorescence (differential fluorescence [DF]) was plotted against the cell fluorescence measured after RNase treatment or against the protein-associated BODIPY 630/650-X or FITC fluorescence.

    Results

    The scattergrams were characteristic of the RNA versus DNA Bivariate distributions where DF represented cellular ds RNA content and fluorescence intensity of the RNase-treated cells, their DNA content. The distributions were used to correlate cellular ds RNA content with the cell cycle position or with protein content.

    Conclusions

    One advantage of this novel approach based on the recording and plotting of DF is that only the RNase -specific fraction of cell fluorescence is measured with no contribution of nonspecific components (e.g., due to the emission spectrum overlap or stainability of other than RNA cell constituents). Another advantage is the method’s simplicity, which ensues from the use of a single dye, the same illumination, and the same emission wavelength detection sensor for measurement of both DNA and ds RNA. The method can be extended for multiparameter Analysis of cell populations stained with other fluorochromes of the same-wavelength emission but targeted (e.g., immunocytochemically) for different cell constituents. Cytometry 45:73–78, 2001. © 2001 Wiley-Liss, Inc.

  • Rapid Communication Bivariate Analysis of Cellular DNA Versus RNA Content by Laser Scanning Cytometry Using the Product of Signal Subtraction (Differential Fluorescence) as a Separate Parameter
    , 2001
    Co-Authors: Piotr Smolewski, Jerzy Grabarek, Louis A. Kamentsky, Zbigniew Darzynkiewicz

    Abstract:

    Bivariate Analysis of Cellular DNA Versus RNA Content by Laser Scanning Cytometry Using the Product of Signal Subtraction (Differential Fluorescence) as a Separate Parameter Piotr Smolewski, Jerzy Grabarek, Louis A. Kamentsky, and Zbigniew Darzynkiewicz* Brander Cancer Research Institute, New York Medical College, Valhalla, New York Department of Hematology, Medical University of Lodz, Lodz, Poland Department of Pathology, Pomeranian School of Medicine, Szczecin, Poland CompuCyte Corporation, Cambridge, Massachusetts

  • Current Protocols in Cytometry – Bivariate Analysis of DNA Content and Expression of Cyclin Proteins
    Current protocols in immunology, 1998
    Co-Authors: G Juan, Zbigniew Darzynkiewicz

    Abstract:

    Cyclins are key components of the cell cycle machinery. This unit describes techniques associated with measurement of these transiently expressed molecules and focusses on the combination of cell cycle Analysis and expression of proliferation-associated proteins via immunocytochemically attached antibodies.

    Keywords:

    flow cytometry;
    cyclins;
    DNA content;
    cell cycle;
    proliferation-associated proteins Cyclins are key components of the cell cycle machinery

Xuechen Zhang – 3rd expert on this subject based on the ideXlab platform

  • Bivariate Analysis of barley scald resistance with relative maturity reveals a new major qtl on chromosome 3h
    Scientific Reports, 2019
    Co-Authors: Xuechen Zhang, Ben Ovenden, Beverley A Orchard, Meixue Zhou, R F Park, Davinder Singh, Andrew Milgate

    Abstract:

    The disease scald of barley is caused by the pathogen Rhynchosporium commune and can cause up to 30–40% yield loss in susceptible cultivars. In this study, the Australian barley cultivar ‘Yerong’ was demonstrated to have resistance that differed from Turk (Rrs1 (Rh3 type)) based on seedling tests with 11 R. commune isolates. A doubled haploid population with 177 lines derived from a cross between ‘Yerong’ and the susceptible Australian cultivar ‘Franklin’ was used to identify quantitative trait loci (QTL) for scald resistance. A QTL on chromosome 3H was identified with large effect, consistent with a major gene conferring scald resistance at the seedling stage. Under field conditions, a Bivariate Analysis was used to model scald percentage of infected leaf area and relative maturity, the residuals from the regression were used as our phenotype for QTL Analysis. This Analysis identified one major QTL on chromosome 3H, which mapped to the same position as the QTL at seedling stage. The identified QTL on 3H is proposed to be different from the Rrs1 on the basis of seedling resistance against different R. commune isolates and physical map position. This study increases the current understanding of scald resistance and identifies genetic material possessing QTLs useful for the marker-assisted selection of scald resistance in barley breeding programs.

  • Bivariate Analysis of barley scald resistance with relative maturity reveals a new major qtl on chromosome 3h
    bioRxiv, 2019
    Co-Authors: Xuechen Zhang, Ben Ovenden, Beverley A Orchard, Meixue Zhou, R F Park, Davinder Singh, Andrew Milgate

    Abstract:

    Abstract The disease scald of barley is caused by the pathogen Rhynchosporium commune and can cause up to 30-40% yield loss in susceptible varieties. In this study, the Australian barley cultivar Yerong was demonstrated to have resistance that differed from Turk (Rrs1) based on seedling tests with 11 R. commune isolates. A doubled haploid population with 177 lines derived from a cross between Yerong and Franklin was used to identify quantitative trait loci (QTL) for scald resistance. Scald resistance against four pathogen isolates was assessed at the seedling growth stage in a glasshouse experiment and at the adult growth stage in field experiments with natural infection over three consecutive years. A QTL on chromosome 3H was identified with large effect, consistent with a major gene conferring scald resistance at the seedling stage. Under field conditions, scald percentage was negatively correlated with early relative maturity. A Bivariate Analysis was used to model scald percentage and relative maturity together, residuals from the regression of scald percentage on relative maturity were used as our phenotype for QTL Analysis. This Analysis identified one major QTL on chromosome 3H, which mapped to the same position as the QTL identified for scald resistance at seedling stage. The identified QTL on 3H is proposed to be different from the Rrs1 on the basis of seedling resistance against different R. commune isolates and physical map position. The Analysis also identified an additional novel QTL on chromosome 7H. This study increases the current understanding of scald resistance and identifies genetic material possessing QTLs useful for the marker-assisted selection of scald resistance in barley breeding programs.