The Experts below are selected from a list of 24483 Experts worldwide ranked by ideXlab platform
Alan B Retik - One of the best experts on this subject based on the ideXlab platform.
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implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human Bladder Muscle
The Journal of Urology, 1993Co-Authors: Anthony Atala, Michael R Freeman, Joseph P Vacanti, Jennifer L Shepard, Alan B RetikAbstract:AbstractThe harvest of human Bladder Muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human Bladder urothelial and Muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and Bladder Muscle cells, when im...
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Implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human Bladder Muscle.
The Journal of urology, 1993Co-Authors: Anthony Atala, Michael R Freeman, Joseph P Vacanti, J Shepard, Alan B RetikAbstract:The harvest of human Bladder Muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human Bladder urothelial and Muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and Bladder Muscle cells, when implanted on polyglycolic acid fibers, from new urological structures in vivo composed of both cell types. The human cell-polymer xenografts can be recovered from host animals at extended times after implantation. These data suggest that feasibility of using polyglycolic acid polymers as substrates for the creation of human urothelial and Muscle grafts for genitourinary reconstruction.
Anthony Atala - One of the best experts on this subject based on the ideXlab platform.
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implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human Bladder Muscle
The Journal of Urology, 1993Co-Authors: Anthony Atala, Michael R Freeman, Joseph P Vacanti, Jennifer L Shepard, Alan B RetikAbstract:AbstractThe harvest of human Bladder Muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human Bladder urothelial and Muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and Bladder Muscle cells, when im...
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Implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human Bladder Muscle.
The Journal of urology, 1993Co-Authors: Anthony Atala, Michael R Freeman, Joseph P Vacanti, J Shepard, Alan B RetikAbstract:The harvest of human Bladder Muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human Bladder urothelial and Muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and Bladder Muscle cells, when implanted on polyglycolic acid fibers, from new urological structures in vivo composed of both cell types. The human cell-polymer xenografts can be recovered from host animals at extended times after implantation. These data suggest that feasibility of using polyglycolic acid polymers as substrates for the creation of human urothelial and Muscle grafts for genitourinary reconstruction.
Tomonori Oura - One of the best experts on this subject based on the ideXlab platform.
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involvement of urinary Bladder connexin43 and the circadian clock in coordination of diurnal micturition rhythm
Nature Communications, 2012Co-Authors: Hiromitsu Negoro, Akihiro Kanematsu, Masao Doi, Sylvia O Suadicani, Masahiro Matsuo, Masaaki Imamura, Takeshi Okinami, Nobuyuki Nishikawa, Tomonori OuraAbstract:Humans and rodents normally store more urine in the Bladder when fast asleep than when awake. In this study, the production of the gap junction protein connexin43, a regulator of Bladder capacity, is shown to oscillate in mouse urinary Bladder Muscle in synchrony with the circadian clock.
Akihiro Kanematsu - One of the best experts on this subject based on the ideXlab platform.
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involvement of urinary Bladder connexin43 and the circadian clock in coordination of diurnal micturition rhythm
Nature Communications, 2012Co-Authors: Hiromitsu Negoro, Akihiro Kanematsu, Masao Doi, Sylvia O Suadicani, Masahiro Matsuo, Masaaki Imamura, Takeshi Okinami, Nobuyuki Nishikawa, Tomonori OuraAbstract:Humans and rodents normally store more urine in the Bladder when fast asleep than when awake. In this study, the production of the gap junction protein connexin43, a regulator of Bladder capacity, is shown to oscillate in mouse urinary Bladder Muscle in synchrony with the circadian clock.
Michael R Freeman - One of the best experts on this subject based on the ideXlab platform.
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implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human Bladder Muscle
The Journal of Urology, 1993Co-Authors: Anthony Atala, Michael R Freeman, Joseph P Vacanti, Jennifer L Shepard, Alan B RetikAbstract:AbstractThe harvest of human Bladder Muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human Bladder urothelial and Muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and Bladder Muscle cells, when im...
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Implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human Bladder Muscle.
The Journal of urology, 1993Co-Authors: Anthony Atala, Michael R Freeman, Joseph P Vacanti, J Shepard, Alan B RetikAbstract:The harvest of human Bladder Muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human Bladder urothelial and Muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and Bladder Muscle cells, when implanted on polyglycolic acid fibers, from new urological structures in vivo composed of both cell types. The human cell-polymer xenografts can be recovered from host animals at extended times after implantation. These data suggest that feasibility of using polyglycolic acid polymers as substrates for the creation of human urothelial and Muscle grafts for genitourinary reconstruction.