The Experts below are selected from a list of 708 Experts worldwide ranked by ideXlab platform
Thomas Brüning - One of the best experts on this subject based on the ideXlab platform.
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Effects of benzo[a]pyrene, aromatic amines, and a combination of both on CYP1A1 activities in RT-4 human Bladder Papilloma cells.
Journal of toxicology and environmental health. Part A, 2016Co-Authors: Sabine Plöttner, Lilian Annika Bastian, Heiko Udo Käfferlein, Thomas BrüningAbstract:ABSTRACTThe interaction of arylamines and polycyclic aromatic hydrocarbons (PAH) is of particular interest in the etiology of Bladder cancer. The aim of this study was to (1) examine the metabolic ...
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Effects of benzo[a]pyrene, aromatic amines, and a combination of both on CYP1A1 activities in RT-4 human Bladder Papilloma cells
2016Co-Authors: Sabine Plöttner, Lilian Annika Bastian, Heiko Udo Käfferlein, Thomas BrüningAbstract:The interaction of arylamines and polycyclic aromatic hydrocarbons (PAH) is of particular interest in the etiology of Bladder cancer. The aim of this study was to (1) examine the metabolic capacity of RT-4 human Bladder Papilloma cells and (2) investigate the influence of aromatic amines on the induction of cytochrome P-450 1A1 (CYP1A1) activity and their effects on benzo[a]pyrene (BaP)-induced CYP1A1 activities. Cells were incubated for 24 h with different concentrations of BaP, 1- or 2-naphthylamine (NA), 2-, 3-, or 4-aminobiphenyl (ABP), and binary mixtures consisting of 1 µM BaP and different concentrations of each arylamine. Changes in CYP1A1 activities were measured at concentrations with no or only low cytotoxicity and accompanied by specific protein detection. Several phase I and II enzymes relevant to metabolism of PAH and arylamines were present in RT-4 cells. Concentration-dependent elevation in CYP1A1 activities accompanied by increasing protein levels was found after treating cells with BaP and 1- or 2-NA. The majority of synergistic effects in binary mixtures were less than additive. In contrast, concentration-dependent inhibition was observed for 2-, 3-, and 4-ABP and in both the presence and absence of BaP. Our results suggest that RT-4 cells represent a reliable model cell line to study arylamine- and PAH-induced effects in vitro and that BaP-induced CYP1A1 activities are modulated by aromatic amines. In general, the direction of the effect depends upon the aromatic amine, rather than being unidirectional for aromatic amines.
Sabine Plöttner - One of the best experts on this subject based on the ideXlab platform.
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Effects of benzo[a]pyrene, aromatic amines, and a combination of both on CYP1A1 activities in RT-4 human Bladder Papilloma cells.
Journal of toxicology and environmental health. Part A, 2016Co-Authors: Sabine Plöttner, Lilian Annika Bastian, Heiko Udo Käfferlein, Thomas BrüningAbstract:ABSTRACTThe interaction of arylamines and polycyclic aromatic hydrocarbons (PAH) is of particular interest in the etiology of Bladder cancer. The aim of this study was to (1) examine the metabolic ...
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Effects of benzo[a]pyrene, aromatic amines, and a combination of both on CYP1A1 activities in RT-4 human Bladder Papilloma cells
2016Co-Authors: Sabine Plöttner, Lilian Annika Bastian, Heiko Udo Käfferlein, Thomas BrüningAbstract:The interaction of arylamines and polycyclic aromatic hydrocarbons (PAH) is of particular interest in the etiology of Bladder cancer. The aim of this study was to (1) examine the metabolic capacity of RT-4 human Bladder Papilloma cells and (2) investigate the influence of aromatic amines on the induction of cytochrome P-450 1A1 (CYP1A1) activity and their effects on benzo[a]pyrene (BaP)-induced CYP1A1 activities. Cells were incubated for 24 h with different concentrations of BaP, 1- or 2-naphthylamine (NA), 2-, 3-, or 4-aminobiphenyl (ABP), and binary mixtures consisting of 1 µM BaP and different concentrations of each arylamine. Changes in CYP1A1 activities were measured at concentrations with no or only low cytotoxicity and accompanied by specific protein detection. Several phase I and II enzymes relevant to metabolism of PAH and arylamines were present in RT-4 cells. Concentration-dependent elevation in CYP1A1 activities accompanied by increasing protein levels was found after treating cells with BaP and 1- or 2-NA. The majority of synergistic effects in binary mixtures were less than additive. In contrast, concentration-dependent inhibition was observed for 2-, 3-, and 4-ABP and in both the presence and absence of BaP. Our results suggest that RT-4 cells represent a reliable model cell line to study arylamine- and PAH-induced effects in vitro and that BaP-induced CYP1A1 activities are modulated by aromatic amines. In general, the direction of the effect depends upon the aromatic amine, rather than being unidirectional for aromatic amines.
Lilian Annika Bastian - One of the best experts on this subject based on the ideXlab platform.
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Effects of benzo[a]pyrene, aromatic amines, and a combination of both on CYP1A1 activities in RT-4 human Bladder Papilloma cells.
Journal of toxicology and environmental health. Part A, 2016Co-Authors: Sabine Plöttner, Lilian Annika Bastian, Heiko Udo Käfferlein, Thomas BrüningAbstract:ABSTRACTThe interaction of arylamines and polycyclic aromatic hydrocarbons (PAH) is of particular interest in the etiology of Bladder cancer. The aim of this study was to (1) examine the metabolic ...
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Effects of benzo[a]pyrene, aromatic amines, and a combination of both on CYP1A1 activities in RT-4 human Bladder Papilloma cells
2016Co-Authors: Sabine Plöttner, Lilian Annika Bastian, Heiko Udo Käfferlein, Thomas BrüningAbstract:The interaction of arylamines and polycyclic aromatic hydrocarbons (PAH) is of particular interest in the etiology of Bladder cancer. The aim of this study was to (1) examine the metabolic capacity of RT-4 human Bladder Papilloma cells and (2) investigate the influence of aromatic amines on the induction of cytochrome P-450 1A1 (CYP1A1) activity and their effects on benzo[a]pyrene (BaP)-induced CYP1A1 activities. Cells were incubated for 24 h with different concentrations of BaP, 1- or 2-naphthylamine (NA), 2-, 3-, or 4-aminobiphenyl (ABP), and binary mixtures consisting of 1 µM BaP and different concentrations of each arylamine. Changes in CYP1A1 activities were measured at concentrations with no or only low cytotoxicity and accompanied by specific protein detection. Several phase I and II enzymes relevant to metabolism of PAH and arylamines were present in RT-4 cells. Concentration-dependent elevation in CYP1A1 activities accompanied by increasing protein levels was found after treating cells with BaP and 1- or 2-NA. The majority of synergistic effects in binary mixtures were less than additive. In contrast, concentration-dependent inhibition was observed for 2-, 3-, and 4-ABP and in both the presence and absence of BaP. Our results suggest that RT-4 cells represent a reliable model cell line to study arylamine- and PAH-induced effects in vitro and that BaP-induced CYP1A1 activities are modulated by aromatic amines. In general, the direction of the effect depends upon the aromatic amine, rather than being unidirectional for aromatic amines.
Heiko Udo Käfferlein - One of the best experts on this subject based on the ideXlab platform.
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Effects of benzo[a]pyrene, aromatic amines, and a combination of both on CYP1A1 activities in RT-4 human Bladder Papilloma cells.
Journal of toxicology and environmental health. Part A, 2016Co-Authors: Sabine Plöttner, Lilian Annika Bastian, Heiko Udo Käfferlein, Thomas BrüningAbstract:ABSTRACTThe interaction of arylamines and polycyclic aromatic hydrocarbons (PAH) is of particular interest in the etiology of Bladder cancer. The aim of this study was to (1) examine the metabolic ...
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Effects of benzo[a]pyrene, aromatic amines, and a combination of both on CYP1A1 activities in RT-4 human Bladder Papilloma cells
2016Co-Authors: Sabine Plöttner, Lilian Annika Bastian, Heiko Udo Käfferlein, Thomas BrüningAbstract:The interaction of arylamines and polycyclic aromatic hydrocarbons (PAH) is of particular interest in the etiology of Bladder cancer. The aim of this study was to (1) examine the metabolic capacity of RT-4 human Bladder Papilloma cells and (2) investigate the influence of aromatic amines on the induction of cytochrome P-450 1A1 (CYP1A1) activity and their effects on benzo[a]pyrene (BaP)-induced CYP1A1 activities. Cells were incubated for 24 h with different concentrations of BaP, 1- or 2-naphthylamine (NA), 2-, 3-, or 4-aminobiphenyl (ABP), and binary mixtures consisting of 1 µM BaP and different concentrations of each arylamine. Changes in CYP1A1 activities were measured at concentrations with no or only low cytotoxicity and accompanied by specific protein detection. Several phase I and II enzymes relevant to metabolism of PAH and arylamines were present in RT-4 cells. Concentration-dependent elevation in CYP1A1 activities accompanied by increasing protein levels was found after treating cells with BaP and 1- or 2-NA. The majority of synergistic effects in binary mixtures were less than additive. In contrast, concentration-dependent inhibition was observed for 2-, 3-, and 4-ABP and in both the presence and absence of BaP. Our results suggest that RT-4 cells represent a reliable model cell line to study arylamine- and PAH-induced effects in vitro and that BaP-induced CYP1A1 activities are modulated by aromatic amines. In general, the direction of the effect depends upon the aromatic amine, rather than being unidirectional for aromatic amines.
Ren Xuan-y - One of the best experts on this subject based on the ideXlab platform.
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Expression of Spl in transitional cell carcinoma of Bladder and its clinical significance
Chinese Journal of Surgical Oncology, 2011Co-Authors: Ren Xuan-yAbstract:Objective To investigate the expression of transcription factor Spl in transitional cell carcinoma of Bladder(BTCC) and analyze its clinical significance.Methods By using immunohistochemical PV-6000 method to study the expression level of Spl in 63 cases of BTCC,21 cases of Papilloma of Bladder and 13 cases normal mucosa of Bladder specimen.Results The positive rate of Spl protein in BTCC(68.3%) was significantly higher than that in Bladder Papilloma(23.8%) and normal Bladder mucosa(7.7%).It had showed that the positive expression of Spl in BTCC was closely related with the pathological grading and TNM stages.Conclusions Transcription factor Spl may play an important role in the carcinogenesis and progression of BTCC.The detection of Spl protein has a definite value in juding the prognosis of BTCC.