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Marija Kocijancic - One of the best experts on this subject based on the ideXlab platform.
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evaluation of the bd vacutainer rst Blood Collection Tube for routine chemistry analytes clinical significance of differences and stability study
Biochemia Medica, 2014Co-Authors: Marija Kocijancic, Jelena Cargonja, Alma DelicknezevicAbstract:INTRODUCTION Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer(®) Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) Blood Collection Tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer(®) RST Blood Collection Tube in reference with the BD Vacutainer(®) Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). MATERIALS AND METHODS Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality. RESULTS Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II Tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C. CONCLUSIONS Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST Tube provides shorter clotting time.
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Evaluation of the BD Vacutainer(®) RST Blood Collection Tube for routine chemistry analytes: clinical significance of differences and stability study.
Biochemia medica, 2014Co-Authors: Marija Kocijancic, Jelena Cargonja, Alma Delic-knezevicAbstract:INTRODUCTION Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer(®) Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) Blood Collection Tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer(®) RST Blood Collection Tube in reference with the BD Vacutainer(®) Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). MATERIALS AND METHODS Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality. RESULTS Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II Tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C. CONCLUSIONS Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST Tube provides shorter clotting time.
Alma Delic-knezevic - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of the BD Vacutainer(®) RST Blood Collection Tube for routine chemistry analytes: clinical significance of differences and stability study.
Biochemia medica, 2014Co-Authors: Marija Kocijancic, Jelena Cargonja, Alma Delic-knezevicAbstract:INTRODUCTION Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer(®) Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) Blood Collection Tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer(®) RST Blood Collection Tube in reference with the BD Vacutainer(®) Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). MATERIALS AND METHODS Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality. RESULTS Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II Tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C. CONCLUSIONS Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST Tube provides shorter clotting time.
Alma Delicknezevic - One of the best experts on this subject based on the ideXlab platform.
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evaluation of the bd vacutainer rst Blood Collection Tube for routine chemistry analytes clinical significance of differences and stability study
Biochemia Medica, 2014Co-Authors: Marija Kocijancic, Jelena Cargonja, Alma DelicknezevicAbstract:INTRODUCTION Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer(®) Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) Blood Collection Tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer(®) RST Blood Collection Tube in reference with the BD Vacutainer(®) Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). MATERIALS AND METHODS Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality. RESULTS Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II Tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C. CONCLUSIONS Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST Tube provides shorter clotting time.
Alan T Remaley - One of the best experts on this subject based on the ideXlab platform.
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interferences from Blood Collection Tube components on clinical chemistry assays
Biochemia Medica, 2014Co-Authors: Raffick A R Bowen, Alan T RemaleyAbstract:Improper design or use of Blood Collection devices can adversely affect the accuracy of laboratory test results. Vascular access devices, such as catheters and needles, exert shear forces during Blood flow, which creates a predisposition to cell lysis. Components from Blood Collection Tubes, such as stoppers, lubricants, surfactants, and separator gels, can leach into specimens and/or adsorb analytes from a specimen; special Tube additives may also alter analyte stability. Because of these interactions with Blood specimens, Blood Collection devices are a potential source of pre-analytical error in laboratory testing. Accurate laboratory testing requires an understanding of the complex interactions between Collection devices and Blood specimens. Manufacturers, vendors, and clinical laboratorians must consider the pre-analytical challenges in laboratory testing. Although other authors have described the effects of endogenous substances on clinical assay results, the effects/impact of Blood Collection Tube additives and components have not been well systematically described or explained. This review aims to identify and describe Blood Collection Tube additives and their components and the strategies used to minimize their effects on clinical chemistry assays.
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Blood Collection Tube-related alterations in analyte concentrations in quality control material and serum specimens
Clinical biochemistry, 2013Co-Authors: Raffick A R Bowen, Annie Sattayapiwat, Verena Gounden, Alan T RemaleyAbstract:Objectives Several previous studies have described the effects of interfering substances on clinical assay results; however, the effects of exogenous substances, particularly additives from Blood Collection Tubes on quality control (QC) specimens and serum specimens have not been well examined. This study examines the effects of Blood-Collection Tube additives on total triiodothyronine (TT3), and thyroxine (TT4), cortisol, and routine clinical chemistry tests in QC and serum specimens from apparently healthy volunteers.
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Immunoassay Interference by a Commonly Used Blood Collection Tube Additive, the Organosilicone Surfactant Silwet L-720
Clinical chemistry, 2005Co-Authors: Raffick A R Bowen, Yung Chan, Mark E. Ruddel, Glen L. Hortin, Gyorgy Csako, Stephen J. Demosky, Alan T RemaleyAbstract:Background: A small number of immunoassays on several different types of analyzers were recently adversely affected by Tube additives in Becton Dickinson (BD) Vacutainer® SST™, SST II, and Microtainer™ Blood Collection Tubes. We examined the effect of a commonly used Tube surfactant, Silwet™ L-720, on immunoassays and the mechanism for the interference. Methods: Immunoassays were performed on serum supplemented with Silwet L-720 on the IMMULITE™ 2500 and AxSYM™ analyzers. Direct effects of the surfactant on the chemiluminescent detection step of immunoassays and on antibody immobilization on the solid phase were examined. Results: Increasing the final surfactant concentration from 0 to 400 mg/L in serum significantly increased (∼51%) the apparent total triiodothyronine (TT3) concentrations measured on the IMMULITE 2500 but not the AxSYM analyzer. Several other competitive, but not noncompetitive, assays were also significantly affected by the surfactant on the IMMULITE 2500 analyzer. The effect was independent of serum components, and the surfactant had no direct effect on chemiluminescence reactions. The capture antibody, however, was displaced from the solid phase by incubation with solutions containing surfactant under conditions similar to the IMMULITE TT3 assay. Conclusions: The Silwet L-720 surfactant, which is used to coat the inner surfaces of Tubes, appears to account for previously reported immunoassay interference by BD Vacutainer SST Blood Collection Tubes. One of the mechanisms for the interference is the desorption of antibodies from the solid phase by the surfactant. The results identify an important factor in the selection of suitable Blood Collection Tube surfactants and provide an approach for solving similar Tube-assay interference problems in the future.
Jelena Cargonja - One of the best experts on this subject based on the ideXlab platform.
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evaluation of the bd vacutainer rst Blood Collection Tube for routine chemistry analytes clinical significance of differences and stability study
Biochemia Medica, 2014Co-Authors: Marija Kocijancic, Jelena Cargonja, Alma DelicknezevicAbstract:INTRODUCTION Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer(®) Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) Blood Collection Tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer(®) RST Blood Collection Tube in reference with the BD Vacutainer(®) Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). MATERIALS AND METHODS Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality. RESULTS Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II Tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C. CONCLUSIONS Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST Tube provides shorter clotting time.
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Evaluation of the BD Vacutainer(®) RST Blood Collection Tube for routine chemistry analytes: clinical significance of differences and stability study.
Biochemia medica, 2014Co-Authors: Marija Kocijancic, Jelena Cargonja, Alma Delic-knezevicAbstract:INTRODUCTION Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer(®) Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) Blood Collection Tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer(®) RST Blood Collection Tube in reference with the BD Vacutainer(®) Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). MATERIALS AND METHODS Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality. RESULTS Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II Tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C. CONCLUSIONS Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST Tube provides shorter clotting time.