The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Matthias Nauck - One of the best experts on this subject based on the ideXlab platform.
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Considerably Reduced Centrifugation Time without Increased Hemolysis: Evaluation of the New BD Vacutainer® SST™II Advance
Clinical chemistry, 2007Co-Authors: Birger Mensel, Ulrike Wenzel, M. Roser, Jan Lüdemann, Matthias NauckAbstract:Short in-laboratory turnaround time is important, and reducing centrifugation time can shorten this phase of the preanalytical process. The new BD Vacutainer® SST™II Advance (Becton Dickinson, article number 367955) has a semisolid, thyxotrophic acrylic gel with chemical composition identical to that of the previous SST™II tube. In the new Advance tube, however, the gel has been extended to 1 side of the tube, forming a gel nose with a larger initial contact surface that is supposed to accelerate gel movement during centrifugation to allow shorter centrifugation time (1). We compared gel barrier formation and hemolysis rates of the new Vacutainer SST™II Advance under a conventional centrifugation (CC) mode (13 min, 1700 g ; acceleration and braking time, 55 s) vs an accelerated centrifugation (AC) mode (5 min, 3000 g ; acceleration and braking time, 39 s) to determine whether this new Vacutainer system allows reduced centrifugation time while maintaining sample quality. In vitro hemolysis can be caused by mechanical stress inflicted by higher g -forces, which might artificially increase several serum analytes and cause interference in some laboratory assays (2). Therefore we measured lactate dehydrogenase …
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considerably reduced centrifugation time without increased hemolysis evaluation of the new bd Vacutainer sst ii advance
Clinical Chemistry, 2007Co-Authors: Birger Mensel, Ulrike Wenzel, M. Roser, Jan Lüdemann, Matthias NauckAbstract:Short in-laboratory turnaround time is important, and reducing centrifugation time can shorten this phase of the preanalytical process. The new BD Vacutainer® SST™II Advance (Becton Dickinson, article number 367955) has a semisolid, thyxotrophic acrylic gel with chemical composition identical to that of the previous SST™II tube. In the new Advance tube, however, the gel has been extended to 1 side of the tube, forming a gel nose with a larger initial contact surface that is supposed to accelerate gel movement during centrifugation to allow shorter centrifugation time (1). We compared gel barrier formation and hemolysis rates of the new Vacutainer SST™II Advance under a conventional centrifugation (CC) mode (13 min, 1700 g ; acceleration and braking time, 55 s) vs an accelerated centrifugation (AC) mode (5 min, 3000 g ; acceleration and braking time, 39 s) to determine whether this new Vacutainer system allows reduced centrifugation time while maintaining sample quality. In vitro hemolysis can be caused by mechanical stress inflicted by higher g -forces, which might artificially increase several serum analytes and cause interference in some laboratory assays (2). Therefore we measured lactate dehydrogenase …
Peter M. George - One of the best experts on this subject based on the ideXlab platform.
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Comparison of BD Vacutainer® Rapid Serum Tube and plasma for haemolysis markers in the emergency department
Annals of Clinical Biochemistry, 2015Co-Authors: J.b. Ryan, Martin Than, Sj Southby, Christopher M. Florkowski, Richard Mackay, La Stuart, Peter M. GeorgeAbstract:BackgroundIn recent years, a rapid-clotting serum tube, BD Vacutainer® Rapid Serum Tube (RST™), was introduced to improve turn-around times for serum samples. Previous studies reported reduced concentrations of some markers of haemolysis in RST specimens compared to other serum or plasma samples. We aimed to compare RST to plasma tubes for haemolysis markers in an emergency department (ED) setting, where increased rates of haemolysis are commonly seen.MethodsPatients presenting to ED over an eight-day period had an RST, BD Vacutainer® PST™ II (plasma) Tube and BD Vacutainer® Heparin (non-gel, plasma) Tube collected. Blood was drawn from an intravenous cannula, and samples were promptly analysed for haemolysis index, potassium, phosphate, aspartate aminotrasferase (AST), magnesium and lactate dehydrogenase (LD).ResultsA total of 347 patient samples were included, and 9.2% of the PST samples were haemolysed. The RST tubes had small increases in all of the haemolysis markers compared to both plasma tubes (P ...
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Evaluation of the BD Vacutainer rapid serum tube on haemolysis markers in the emergency department
Pathology, 2014Co-Authors: J.b. Ryan, Martin Than, Lesney A. Stuart, Sj Southby, Christopher M. Florkowski, Richard Mackay, Peter M. GeorgeAbstract:Aims Haemolysis is an important source of assay interference, especially in the Emergency Department (ED). A rapid-clotting serum tube, Becton-Dickinson (BD) Vacutainer Rapid Serum Tube (RST), was reported to reduce haemolysis rates compared to standard serum tubes. We evaluated the effect of RST on haemolysis markers compared with our current STAT testing tube, BD Vacutainer Plasma Separator Tube (PST). Methods Patients presenting to the ED over a 8-day period had an additional RST and BD Vacutainer non-gel heparin plasma tube collected. Blood was collected from 347 patients by trained nursing staff from an intravenous cannula using an adaptor device (Vacutainer Luer-Lok access device). All samples were then analysed for six haemolysis markers: haemolysis index, phosphate, magnesium, potassium, lactate dehydrogenase (LD), and aspartate aminotras-ferase (AST). Results We found 9% of samples were haemolysed (>0.5g/L free haemoglobin). Significant differences (p p 0.05) higher for phosphate and potassium and lower for LD. Discussion/conclusion RST tubes did not improve markers of haemolysis compared to plasma tubes.
Norbert Blanckaert - One of the best experts on this subject based on the ideXlab platform.
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studies on the use of bd Vacutainer sst ii and rst in general practice investigation of artefactual hyperkalaemia
Annals of Clinical Biochemistry, 2014Co-Authors: Tine Huyghe, Frank Buntinx, Rudi Bruyninckx, Veerle Besard, Jos Vunckx, Stephen Church, Karen Byron, Renee Rosa, Norbert BlanckaertAbstract:Background: Current sampling and transport conditions of samples in general practice can result in pseudohyperkalaemia. This study was undertaken to determine, in a general practice setting, whether there is any difference in haemolysis obtained when using BD Vacutainer Rapid Serum Tubes (BD RST) compared with using BD Vacutainer
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Studies on the use of BD Vacutainer® SST II™ and RST™ in general practice: investigation of artefactual hyperkalaemia
Annals of Clinical Biochemistry: An international journal of biochemistry and laboratory medicine, 2013Co-Authors: Tine Huyghe, Frank Buntinx, Rudi Bruyninckx, Veerle Besard, Jos Vunckx, Stephen Church, Karen Byron, Renee Rosa, Norbert BlanckaertAbstract:Background: Current sampling and transport conditions of samples in general practice can result in pseudohyperkalaemia. This study was undertaken to determine, in a general practice setting, whether there is any difference in haemolysis obtained when using BD Vacutainer Rapid Serum Tubes (BD RST) compared with using BD Vacutainer
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evaluation of the bd Vacutainer pst ii blood collection tube for special chemistry analytes
Clinical Chemistry and Laboratory Medicine, 2009Co-Authors: Jeffrey J Chance, Julie Berube, Marita Vandersmissen, Norbert BlanckaertAbstract:Background: The performance of the BD Vacutainer® PST™ II Tube for testing of special chemistry analytes was evaluated in comparison to serum and heparin plasma non-gel blood collection tubes. The comparison tubes included the BD Vacutainer® Serum Plus Tube, the BD Vacutainer® Lithium Heparin Plus Tube, and the BD Vacutainer® Lithium Heparin Glass Tube. Methods: Tubes were drawn by routine venipuncture from 42 subjects according to a randomized draw order. Tubes were processed and centrifuged according to recommended handling procedures. Serum and plasma from the comparison tubes were aliquoted to secondary containers prior to analysis. Specimens were then tested for selected special chemistry analytes at two time intervals (initial time and after 24 h storage). Analytes tested included thyroid stimulating hormone, free thyroxine, total thyroxine, follicle stimulating hormone, luteinizing hormone, ferritin, cortisol, vitamin B12, folate, and testosterone. The data were collected and analyzed by analysis of variance and mean bias comparisons. Results: The performance of the BD Vacutainer® PST™ II Tube was considered to be clinically equivalent to all three comparison tubes for all assays. Test results from the BD Vacutainer® PST™ II Tube and from aliquots from the three comparison tubes at 24 h were considered to be clinically equivalent to those at initial time for all assays. Conclusions: The BD Vacutainer® PST™ II Tube provided clinically equivalent results to serum and plasma non-gel tubes and good storage stability for the assays evaluated without the need to aliquot.
Birger Mensel - One of the best experts on this subject based on the ideXlab platform.
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Considerably Reduced Centrifugation Time without Increased Hemolysis: Evaluation of the New BD Vacutainer® SST™II Advance
Clinical chemistry, 2007Co-Authors: Birger Mensel, Ulrike Wenzel, M. Roser, Jan Lüdemann, Matthias NauckAbstract:Short in-laboratory turnaround time is important, and reducing centrifugation time can shorten this phase of the preanalytical process. The new BD Vacutainer® SST™II Advance (Becton Dickinson, article number 367955) has a semisolid, thyxotrophic acrylic gel with chemical composition identical to that of the previous SST™II tube. In the new Advance tube, however, the gel has been extended to 1 side of the tube, forming a gel nose with a larger initial contact surface that is supposed to accelerate gel movement during centrifugation to allow shorter centrifugation time (1). We compared gel barrier formation and hemolysis rates of the new Vacutainer SST™II Advance under a conventional centrifugation (CC) mode (13 min, 1700 g ; acceleration and braking time, 55 s) vs an accelerated centrifugation (AC) mode (5 min, 3000 g ; acceleration and braking time, 39 s) to determine whether this new Vacutainer system allows reduced centrifugation time while maintaining sample quality. In vitro hemolysis can be caused by mechanical stress inflicted by higher g -forces, which might artificially increase several serum analytes and cause interference in some laboratory assays (2). Therefore we measured lactate dehydrogenase …
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considerably reduced centrifugation time without increased hemolysis evaluation of the new bd Vacutainer sst ii advance
Clinical Chemistry, 2007Co-Authors: Birger Mensel, Ulrike Wenzel, M. Roser, Jan Lüdemann, Matthias NauckAbstract:Short in-laboratory turnaround time is important, and reducing centrifugation time can shorten this phase of the preanalytical process. The new BD Vacutainer® SST™II Advance (Becton Dickinson, article number 367955) has a semisolid, thyxotrophic acrylic gel with chemical composition identical to that of the previous SST™II tube. In the new Advance tube, however, the gel has been extended to 1 side of the tube, forming a gel nose with a larger initial contact surface that is supposed to accelerate gel movement during centrifugation to allow shorter centrifugation time (1). We compared gel barrier formation and hemolysis rates of the new Vacutainer SST™II Advance under a conventional centrifugation (CC) mode (13 min, 1700 g ; acceleration and braking time, 55 s) vs an accelerated centrifugation (AC) mode (5 min, 3000 g ; acceleration and braking time, 39 s) to determine whether this new Vacutainer system allows reduced centrifugation time while maintaining sample quality. In vitro hemolysis can be caused by mechanical stress inflicted by higher g -forces, which might artificially increase several serum analytes and cause interference in some laboratory assays (2). Therefore we measured lactate dehydrogenase …
Mark Holodniy - One of the best experts on this subject based on the ideXlab platform.
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Comparative stabilities of quantitative human immunodeficiency virus RNA in plasma from samples collected in Vacutainer CPT, Vacutainer PPT, and standard Vacutainer tubes.
Journal of clinical microbiology, 1995Co-Authors: Mark Holodniy, Larry A. Mole, B Yen-lieberman, D Margolis, C Starkey, R Carroll, T Spahlinger, J Todd, J B JacksonAbstract:This study compared the levels of human immunodeficiency virus (HIV) virion RNA in plasma from whole blood collected in Vacutainer CPT (cell preparation tube), Vacutainer PPT (plasma preparation tube), Vacutainer SST (serum separation tube), and standard Vacutainer tubes with sodium heparin, acid citrate dextrose, sodium citrate, and potassium EDTA used as anticoagulants. Quantitative plasma HIV RNA levels were measured by branched-DNA signal amplification. Blood from all tubes was either processed within 1 to 3 h after collection or stored at room temperature or at 4 degrees C for analysis at 6 to 8 and 30 h postdraw. Immediately separated plasma from sodium citrate CPT tubes held at 4 degrees C maintained better stability of HIV RNA equivalents than whole blood held at room temperature or 4 degrees C. The highest number of HIV RNA equivalents was seen with EDTA Vacutainer tubes. HIV RNA equivalents in all types of plasma were significantly higher than in SST tubes. Although a decline in HIV RNA equivalents was seen in all collection devices after 30 h, a significantly greater decline in plasma HIV RNA equivalents occurred in acid citrate dextrose Vacutainer tubes than in citrate CPT, PPT, and standard EDTA Vacutainer tubes. In order to minimize the variability of quantitative HIV RNA test results, our data suggest that samples collected for a particular assay should be processed at the same time postdraw using a particular tube type throughout a given study.
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stabilities of quantitative plasma culture for human immunodeficiency virus rna and p24 antigen from samples collected in Vacutainer cpt and standard Vacutainer tubes
Journal of Clinical Microbiology, 1994Co-Authors: Larry A. Mole, D Margolis, R Carroll, J Todd, Mark HolodniyAbstract:We evaluated the stability of human immunodeficiency virus (HIV) load markers from blood samples collected in Vacutainer CPT or standard Vacutainer brand tubes using sodium heparin or sodium citrate as anticoagulants. Quantitative plasma culture and p24 antigen concentrations were determined, and HIV RNA levels in plasma were measured by both reverse transcription-PCR-enzyme-linked immunosorbent assay (RT-PCR-ELISA) and branched DNA methods. All tubes were stored at room temperature for analysis at 2, 24, 48, and 72 h after the blood samples were drawn. No difference was seen between tube types with respect to the HIV titer in plasma or the positivity rate for all samples that demonstrated a fall in titer over time. Unbound p24 antigen levels in plasma decreased during the initial 48-h period in both tube types. Immune complex-dissociated p24 antigen levels decreased in CPT tubes but not in standard Vacutainer tubes. The HIV RNA copy number in plasma measured by RT-PCR-ELISA was stable in most subjects and was significantly higher in CPT tubes than in standard Vacutainer tubes at 24 and 72 h after the blood samples were drawn. The branched DNA probe assay detected a significant decline in HIV RNA equivalent in plasma over 72 h in both collection tubes, the decline being more dramatic in the standard Vacutainer tube than the CPT tube. Overall, interday variability suggests that samples collected for a particular assay should be processed at the same time after blood is drawn and that a particular tube type be used throughout a given study.
