The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform

William Michael Dunne - One of the best experts on this subject based on the ideXlab platform.

S. Nanua - One of the best experts on this subject based on the ideXlab platform.

Annemerethe Hanssen - One of the best experts on this subject based on the ideXlab platform.

  • culturing periprosthetic tissue in bact alert virtuo Blood Culture System leads to improved and faster detection of prosthetic joint infections
    BMC Infectious Diseases, 2019
    Co-Authors: Adriana Sanabria, Merethe E O Rokeberg, Mona Johannessen, Johanna Ericson U Sollid, Gunnar Skov Simonsen, Annemerethe Hanssen
    Abstract:

    Blood Culture bottles (BCBs) provide a semiautomated method for culturing periprosthetic tissue specimens. A study evaluating BCBs for culturing clinical samples other than body fluids is needed before implementation into clinical practice. Our objective was to evaluate use of the BacT/Alert® Virtuo Blood Culture System for culturing periprosthetic tissue specimens. The study was performed through the analysis of spiked (n = 36) and clinical (n = 158) periprosthetic tissue samples. Clinical samples were analyzed by the BCB method and the results were compared to the conventional microbiological Culture-based method for time to detection and microorganisms identified. The BacT/Alert® Virtuo Blood Culture System detected relevant bacteria for prosthetic joint infection in both spiked and clinical samples. The BCB method was found to be as sensitive (79%) as the conventional method (76%) (p = 0.844) during the analyses of clinical samples. The BCB method yielded positive results much faster than the conventional method: 89% against 27% detection within 24 h, respectively. The median detection time was 11.1 h for the BCB method (12 h and 11 h for the aerobic and the anaerobic BCBs, correspondingly). We recommend using the BacT/Alert® Virtuo Blood Culture System for analyzing prosthetic joint tissue, since this detect efficiently and more rapidly a wider range of bacteria than the conventional microbiological method.

  • Culturing periprosthetic tissue in BacT/Alert® Virtuo Blood Culture System leads to improved and faster detection of prosthetic joint infections
    BMC infectious diseases, 2019
    Co-Authors: Adriana Sanabria, Merethe E O Rokeberg, Mona Johannessen, Johanna Ericson U Sollid, Gunnar Skov Simonsen, Annemerethe Hanssen
    Abstract:

    Blood Culture bottles (BCBs) provide a semiautomated method for culturing periprosthetic tissue specimens. A study evaluating BCBs for culturing clinical samples other than body fluids is needed before implementation into clinical practice. Our objective was to evaluate use of the BacT/Alert® Virtuo Blood Culture System for culturing periprosthetic tissue specimens. The study was performed through the analysis of spiked (n = 36) and clinical (n = 158) periprosthetic tissue samples. Clinical samples were analyzed by the BCB method and the results were compared to the conventional microbiological Culture-based method for time to detection and microorganisms identified. The BacT/Alert® Virtuo Blood Culture System detected relevant bacteria for prosthetic joint infection in both spiked and clinical samples. The BCB method was found to be as sensitive (79%) as the conventional method (76%) (p = 0.844) during the analyses of clinical samples. The BCB method yielded positive results much faster than the conventional method: 89% against 27% detection within 24 h, respectively. The median detection time was 11.1 h for the BCB method (12 h and 11 h for the aerobic and the anaerobic BCBs, correspondingly). We recommend using the BacT/Alert® Virtuo Blood Culture System for analyzing prosthetic joint tissue, since this detect efficiently and more rapidly a wider range of bacteria than the conventional microbiological method.

Kevin C. Hazen - One of the best experts on this subject based on the ideXlab platform.

  • multicenter clinical evaluation of bact alert virtuo Blood Culture System
    Journal of Clinical Microbiology, 2017
    Co-Authors: Michael R. Jacobs, Tony Mazzulli, Kevin C. Hazen, Caryn E. Good, Ayman M. Abdelhamed, Bianche Shum, Katharine P. Roman, Danielle C. Robinson
    Abstract:

    BacT/Alert Virtuo is an advanced, automated Blood Culture System incorporating improved automation and an enhanced detection algorithm to shorten time to detection. A multicenter study of the investigational Virtuo System (bioMerieux, Inc., Durham, NC) compared to BacT/Alert 3D (BTA3D) for detection of bacteremia/fungemia in four bottle types, SA and FA Plus (aerobic) and SN and FN Plus (anaerobic), was performed in a clinical setting with patient samples in a matched System design clinical trial. Blood was added to paired aerobic or anaerobic bottles, with the volume in each bottle in each pair required to be ≤10 ml and with the volumes required to be within 30% of each other. Of 5,709 bottle sets (52.5% aerobic pairs and 47.5% anaerobic pairs), 430 (7.5%) were positive for bacterial or fungal growth, with 342 (6.0%) clinically significant and 83 (1.5%) contaminated. A total of 3,539 sets (62.0%) were volume compliant, with 203 sets (5.7%) clinically significant. The positivity rates for volume-compliant bottle pairs determined by the two Systems were comparable, with 68.7% of clinically significant isolates detected by both instruments, 15.7% by Virtuo only, and 15.7% by BTA3D only. Virtuo detected microbial growth nearly 2 h sooner overall than BTA3D (mean, 15.9 h versus 17.7 h). Shorter time to detection by Virtuo was related to organism group, with the time to detection being significantly shorter for enteric Gram-negative bacilli and enterococci (means, 3.6 h and 2.3 h shorter, respectively). This large clinical study demonstrated that the Virtuo Blood Culture System produced results comparable to those seen with the long-established BTA3D System, with significantly shorter time to detection.

  • Multicenter Clinical Evaluation of BacT/Alert Virtuo Blood Culture System
    Journal of clinical microbiology, 2017
    Co-Authors: Michael R. Jacobs, Tony Mazzulli, Kevin C. Hazen, Caryn E. Good, Ayman M. Abdelhamed, Bianche Shum, Katharine P. Roman, Danielle C. Robinson
    Abstract:

    BacT/Alert Virtuo is an advanced, automated Blood Culture System incorporating improved automation and an enhanced detection algorithm to shorten time to detection. A multicenter study of the investigational Virtuo System (bioMerieux, Inc., Durham, NC) compared to BacT/Alert 3D (BTA3D) for detection of bacteremia/fungemia in four bottle types, SA and FA Plus (aerobic) and SN and FN Plus (anaerobic), was performed in a clinical setting with patient samples in a matched System design clinical trial. Blood was added to paired aerobic or anaerobic bottles, with the volume in each bottle in each pair required to be ≤10 ml and with the volumes required to be within 30% of each other. Of 5,709 bottle sets (52.5% aerobic pairs and 47.5% anaerobic pairs), 430 (7.5%) were positive for bacterial or fungal growth, with 342 (6.0%) clinically significant and 83 (1.5%) contaminated. A total of 3,539 sets (62.0%) were volume compliant, with 203 sets (5.7%) clinically significant. The positivity rates for volume-compliant bottle pairs determined by the two Systems were comparable, with 68.7% of clinically significant isolates detected by both instruments, 15.7% by Virtuo only, and 15.7% by BTA3D only. Virtuo detected microbial growth nearly 2 h sooner overall than BTA3D (mean, 15.9 h versus 17.7 h). Shorter time to detection by Virtuo was related to organism group, with the time to detection being significantly shorter for enteric Gram-negative bacilli and enterococci (means, 3.6 h and 2.3 h shorter, respectively). This large clinical study demonstrated that the Virtuo Blood Culture System produced results comparable to those seen with the long-established BTA3D System, with significantly shorter time to detection.

  • Potential use of BacT/Alert automated Blood Culture System for antifungal susceptibility testing.
    Journal of clinical microbiology, 1994
    Co-Authors: Kevin C. Hazen, M. P. Chery, Yongmoon Han
    Abstract:

    A simple, rapid susceptibility test is needed to determine the possible resistance of yeasts to commonly used antifungal agents. The BacT/Alert automated Blood Culture System was evaluated as one technology for development of such a test. Yeast nitrogen base was used as the growth medium, and amphotericin B was the test antifungal agent. Isolates of various Candida species, Torulopsis glabrata, Saccharomyces cerevisiae, and Cryptococcus neoformans were evaluated. The results suggest that detection of amphotericin B resistance of yeast isolates within 12 to 14 h after inoculation of test medium is possible.

Volkan Özenci - One of the best experts on this subject based on the ideXlab platform.