The Experts below are selected from a list of 55359 Experts worldwide ranked by ideXlab platform
Jian-hua Liang - One of the best experts on this subject based on the ideXlab platform.
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Pterostilbene, an active component of the dragon's Blood Extract, acts as an antidepressant in adult rats.
Psychopharmacology, 2019Co-Authors: Liang Yang, Yuanyuan Ran, Zhenzhen Quan, Ran Wang, Qinghu Yang, Qiutian Jia, Yiheng Peng, Jian-hua LiangAbstract:Background Hippocampal neurogenesis has been widely considered as one of the potential biological mechanisms for the treatment of depression caused by chronic stress. Many natural products have been reported to be beneficial for neurogenesis. Objectives The present study is designed to investigate the effect of dragon's Blood Extract (DBE) and its biologically active compound, pterostilbene (PTE), on hippocampal neurogenesis. Methods The male Sprague-Dawley (SD) rats were used in this study, which were maintained on the normal, DBE and PTE diet groups for 4 weeks before dissection in the normal rat model and behavioral testing in the CUS depression rat model. Meanwhile, DMI-treated rats are subcutaneously injected with DMI (10 mg/kg, i.p.). Results Results revealed that DBE and PTE have the ability to promote hippocampal neurogenesis. DBE and PTE also promoted the proliferation of neural stem cells isolated from the brain of suckling rats. Oral administration of DBE and PTE induced the proliferation, migration, and differentiation of neural progenitor cells (NPCs) in chronic unexpected stressed (CUS) model rats, and improved the behavioral ability and alleviated depress-like symptoms of CUS rats. It was also observed that PTE treatment significantly induced the expression of neurogenesis-related factors, including BDNF, pERK, and pCREB. Conclusion Oral administration of PTE could affect neurogenesis and it is likely to be achieved via BDNF/ERK/CREB-associated signaling pathways.
Junxu Fang - One of the best experts on this subject based on the ideXlab platform.
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Effect of protein-free calf Blood Extract gel combined with sodium hyaluronate eye drops in the treatment of dry eyes after phacoemulsification
Chinese Journal of Primary Medicine and Pharmacy, 2016Co-Authors: Junxu FangAbstract:Objective To evaluate the clinical efficacy of protein-free calf Blood Extract gel combined with sodium hyaluronate eye drops on the dry eyes after phacoemulsification. Methods 80 patients (80 eyes) with dry eye who were diagnosed after phacoemulsification were selected, and they were randomly divided into observation group and control group, 40 cases in each group.The observation group was treated with protein-free calf Blood Extract gel combined with sodium hyaluronate eye drops, and the control group was treated only by sodium hyaluronate eye drops.Schirmer I test, tear film breakup time(BUT) and subjective symptom scores in two groups before and after two weeks treatment were observed. Results The Schirmer I test, BUT, subjective symptom scores of pre-treatment between the two groups had no significant differences.The Schirmer I test, BUT, subjective symptom scores of the observation group after treatment were (4.88±0.85)mm, (8.50±2.10)s, (3.00±2.25)unit, which of the control group were (4.63±1.00)mm, (5.58±1.57)s, (5.10±2.11)unit.The BUT, subjective symptom scores of pre-and post-treatment between the two groups had significant differences(t=-7.06, 4.303, all P
Lv Lan - One of the best experts on this subject based on the ideXlab platform.
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Experiment study of bred cell calf Blood Extract eye gels in the treatment of rabbit corneal ulcer rehabilitation
The Chinese Journal of Clinical Pharmacology, 2011Co-Authors: Lv LanAbstract:Objective To observe the effect of bred cell calf Blood Extract eye gels in the treatment of rehabilitation of rabbit corneal unlcer and neovascularzation of the cornea.Methods One hundred μm depth sterile corneal ulcer was made in the center of right eye of all rabbit with 8.5 mm diameter trephine.Forty rabbits were randomly devided into four groups(10 rabbits for each group),that was 0.9% normal salin group(model group),bFGF eye gel group(controlⅠgroup),protein-free calf Blood Extract eye gel group(control Ⅱgroup) and bred cell calf Blood Extract eye gels group(test group).All medication was used four times per day and continued for 28 days.Epithelial defect restoration and cornea neovascularzation,etc.were observed and documented on 7,14,21,28 d after treatment.Results At the end of the observation-period,there was no significant differencen between test group and other positive control groups(P0.05),but test group shows less neovascularzation than other positive control groups(P0.05).Conclusion Bred cell calf Blood Extract eye gels has marked facilitation effect in the healing course of rabbits's corneal ulcer without much neovascularzation.
Pierre Wallemacq - One of the best experts on this subject based on the ideXlab platform.
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hplc microparticle enzyme immunoassay specific for tacrolimus in whole Blood of hepatic and renal transplant patients
Clinical Chemistry, 1995Co-Authors: I Firdaous, T. Besse, Jean-bernard Otte, Abeer Hassoun, Jean-paul Squifflet, Raymond Reding, Pierre WallemacqAbstract:Tacrolimus is a relatively new immunosuppressant used in organ transplantation to prevent graft rejection. However, its use is not devoid of side effects, making it important to maintain Blood concentrations within therapeutic ranges. Several analytical methods are currently available for routine drug monitoring. However, these methods are based on use of the same monoclonal antibody, which also cross-reacts with some metabolites, resulting in overestimation of some Blood concentrations. Even though this antibody appears appropriate for therapeutic drug monitoring, no reference method measures only the parent drug, mainly because of the poor absorptivity of tacrolimus in ultraviolet light. We have developed a method displaying an increased specificity towards the unchanged drug, using conventional equipment available in most clinical laboratories. After chromatographic separation of the Blood Extract, the tacrolimus fraction is analyzed by an automated microparticle enzyme immunoassay (MEIA) performed on the IMx analyzer (Abbott Labs.). This method is linear from 0 to 40 micrograms/L, yields CVs from 8.5% to 18.2%, and has a detection limit of 5 micrograms/L. Tacrolimus concentrations obtained by HPLC-MEIA in hepatic and renal transplant patients are from 47.5% to 18.8% lower than those obtained by MEIA, according to liver function tests and metabolite accumulation, even though no significant differences were observed between the methods for drug-free Blood samples supplemented with known amounts of tacrolimus.
Liang Yang - One of the best experts on this subject based on the ideXlab platform.
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Pterostilbene, an active component of the dragon's Blood Extract, acts as an antidepressant in adult rats.
Psychopharmacology, 2019Co-Authors: Liang Yang, Yuanyuan Ran, Zhenzhen Quan, Ran Wang, Qinghu Yang, Qiutian Jia, Yiheng Peng, Jian-hua LiangAbstract:Background Hippocampal neurogenesis has been widely considered as one of the potential biological mechanisms for the treatment of depression caused by chronic stress. Many natural products have been reported to be beneficial for neurogenesis. Objectives The present study is designed to investigate the effect of dragon's Blood Extract (DBE) and its biologically active compound, pterostilbene (PTE), on hippocampal neurogenesis. Methods The male Sprague-Dawley (SD) rats were used in this study, which were maintained on the normal, DBE and PTE diet groups for 4 weeks before dissection in the normal rat model and behavioral testing in the CUS depression rat model. Meanwhile, DMI-treated rats are subcutaneously injected with DMI (10 mg/kg, i.p.). Results Results revealed that DBE and PTE have the ability to promote hippocampal neurogenesis. DBE and PTE also promoted the proliferation of neural stem cells isolated from the brain of suckling rats. Oral administration of DBE and PTE induced the proliferation, migration, and differentiation of neural progenitor cells (NPCs) in chronic unexpected stressed (CUS) model rats, and improved the behavioral ability and alleviated depress-like symptoms of CUS rats. It was also observed that PTE treatment significantly induced the expression of neurogenesis-related factors, including BDNF, pERK, and pCREB. Conclusion Oral administration of PTE could affect neurogenesis and it is likely to be achieved via BDNF/ERK/CREB-associated signaling pathways.