The Experts below are selected from a list of 1854 Experts worldwide ranked by ideXlab platform
Antonia M. Joussen - One of the best experts on this subject based on the ideXlab platform.
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Breakdown of the Blood-Eye Barrier in choroidal melanoma after proton beam radiotherapy
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Annette Hager, Friederike Meissner, Aline–isabel Riechardt, Theresa Bonaventura, Julia Löwen, Jens Heufelder, Antonia M. JoussenAbstract:Purpose Irradiation of choroidal melanoma is a safe and globe preserving procedure. Chronic inflammatory processes and ischemia are the main reasons for secondary enucleation in the long run. The aim of this study was to determine whether intraocular inflammation and especially inflammatory response after proton beam therapy (PBT) is related to primary tumor characteristics such as height, tumor volume, and initial flare values. Methods Twenty-six patients treated for uveal melanoma using PBT were included. All patients were examined for signs of inflammation using laser flare photometry (LFP). Each examination included assessment of the melanoma and fellow Eye (which acted as the control) and imaging of the melanoma. Results Significant differences of flare values between melanoma Eyes and control group were found both at baseline (median 17.65 ph/ms (min 4, max 37.10), 7.45 ph/ms (min 0.80, max 16.40), respectively) and during follow-up (median 21.45 ph/ms (min 4.5, max 70.90); 6.05 ph/ms (min 2.40, max 16.40), respectively) ( p
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breakdown of the Blood Eye Barrier in choroidal melanoma after proton beam radiotherapy
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Annette Hager, Friederike Meissner, Aline–isabel Riechardt, Theresa Bonaventura, Julia Löwen, Jens Heufelder, Antonia M. JoussenAbstract:PURPOSE Irradiation of choroidal melanoma is a safe and globe preserving procedure. Chronic inflammatory processes and ischemia are the main reasons for secondary enucleation in the long run. The aim of this study was to determine whether intraocular inflammation and especially inflammatory response after proton beam therapy (PBT) is related to primary tumor characteristics such as height, tumor volume, and initial flare values. METHODS Twenty-six patients treated for uveal melanoma using PBT were included. All patients were examined for signs of inflammation using laser flare photometry (LFP). Each examination included assessment of the melanoma and fellow Eye (which acted as the control) and imaging of the melanoma. RESULTS Significant differences of flare values between melanoma Eyes and control group were found both at baseline (median 17.65 ph/ms (min 4, max 37.10), 7.45 ph/ms (min 0.80, max 16.40), respectively) and during follow-up (median 21.45 ph/ms (min 4.5, max 70.90); 6.05 ph/ms (min 2.40, max 16.40), respectively) (p < 0.001, Wilcoxon test). Flare values in melanoma Eyes increased significantly after PBT (p = 0.005, Wilcoxon test) and after a follow-up of 94 days (median, 7-420 days). Flare values of the control group did not change (p = 0.946, Wilcoxon test). The increase of flare values correlated significantly with maximum tumor height and volume (Spearman-Rho 0.633, p = 0.001 and 0.519, p = 0.007, respectively). CONCLUSION LFP has proven to show significantly higher flare values in melanoma Eyes as compared with the control group and provides data on the course of the inflammatory response after treatment. It may ease treatment planning both at baseline and during follow-up.
Annette Hager - One of the best experts on this subject based on the ideXlab platform.
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Breakdown of the Blood-Eye Barrier in choroidal melanoma after proton beam radiotherapy
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Annette Hager, Friederike Meissner, Aline–isabel Riechardt, Theresa Bonaventura, Julia Löwen, Jens Heufelder, Antonia M. JoussenAbstract:Purpose Irradiation of choroidal melanoma is a safe and globe preserving procedure. Chronic inflammatory processes and ischemia are the main reasons for secondary enucleation in the long run. The aim of this study was to determine whether intraocular inflammation and especially inflammatory response after proton beam therapy (PBT) is related to primary tumor characteristics such as height, tumor volume, and initial flare values. Methods Twenty-six patients treated for uveal melanoma using PBT were included. All patients were examined for signs of inflammation using laser flare photometry (LFP). Each examination included assessment of the melanoma and fellow Eye (which acted as the control) and imaging of the melanoma. Results Significant differences of flare values between melanoma Eyes and control group were found both at baseline (median 17.65 ph/ms (min 4, max 37.10), 7.45 ph/ms (min 0.80, max 16.40), respectively) and during follow-up (median 21.45 ph/ms (min 4.5, max 70.90); 6.05 ph/ms (min 2.40, max 16.40), respectively) ( p
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breakdown of the Blood Eye Barrier in choroidal melanoma after proton beam radiotherapy
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Annette Hager, Friederike Meissner, Aline–isabel Riechardt, Theresa Bonaventura, Julia Löwen, Jens Heufelder, Antonia M. JoussenAbstract:PURPOSE Irradiation of choroidal melanoma is a safe and globe preserving procedure. Chronic inflammatory processes and ischemia are the main reasons for secondary enucleation in the long run. The aim of this study was to determine whether intraocular inflammation and especially inflammatory response after proton beam therapy (PBT) is related to primary tumor characteristics such as height, tumor volume, and initial flare values. METHODS Twenty-six patients treated for uveal melanoma using PBT were included. All patients were examined for signs of inflammation using laser flare photometry (LFP). Each examination included assessment of the melanoma and fellow Eye (which acted as the control) and imaging of the melanoma. RESULTS Significant differences of flare values between melanoma Eyes and control group were found both at baseline (median 17.65 ph/ms (min 4, max 37.10), 7.45 ph/ms (min 0.80, max 16.40), respectively) and during follow-up (median 21.45 ph/ms (min 4.5, max 70.90); 6.05 ph/ms (min 2.40, max 16.40), respectively) (p < 0.001, Wilcoxon test). Flare values in melanoma Eyes increased significantly after PBT (p = 0.005, Wilcoxon test) and after a follow-up of 94 days (median, 7-420 days). Flare values of the control group did not change (p = 0.946, Wilcoxon test). The increase of flare values correlated significantly with maximum tumor height and volume (Spearman-Rho 0.633, p = 0.001 and 0.519, p = 0.007, respectively). CONCLUSION LFP has proven to show significantly higher flare values in melanoma Eyes as compared with the control group and provides data on the course of the inflammatory response after treatment. It may ease treatment planning both at baseline and during follow-up.
A. F. De Vos - One of the best experts on this subject based on the ideXlab platform.
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Retinal Pigment Epithelium-immune System Interactions: Cytokine Production and Cytokine-induced Changes
Progress in retinal and eye research, 2001Co-Authors: G M Holtkamp, Aize Kijlstra, R Peek, A. F. De VosAbstract:Vision is dependent on proper function of several intraocular structures. Immune responses to eliminate invading pathogens from the Eye may threat vision by causing damage to these structures. Therefore, immunological defence of the Eye should be carefully balanced between efficacy and maintenance of functional integrity. The Eye is equipped with several regulatory mechanisms to prevent certain immune and inflammatory responses and is, therefore, regarded as an immune privileged site. The retinal pigment epithelium (RPE) contributes to the immune privileged status of the Eye as part of the Blood-Eye Barrier and by the secretion of immunosuppressive factors inside the Eye. RPE cells, however, may also play an important role in the development of immune and inflammatory responses in the posterior part of the Eye. During the last decade it has become clear that RPE cells are highly sensitive to a variety of inflammatory cytokines. Under inflammatory conditions, RPE cells produce a myriad of cytokines that may activate the resident ocular cells or attract and activate leukocytes. Cytokine stimulation of RPE cells causes profound effects, including nitric oxide secretion, cell surface expression of MHC class II and adhesion molecules and abrogation of Barrier function. This article provides a comprehensive review of the literature concerning RPE cells and cytokines.
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Analysis of the secretion pattern of monocyte chemotactic protein‐1 (MCP‐1) and transforming growth factor‐beta 2 (TGF‐β2) by human retinal pigment epithelial cells
Clinical and experimental immunology, 1999Co-Authors: G M Holtkamp, R Peek, A. F. De Vos, A KijlstaAbstract:Retinal pigment epithelial (RPE) cells, situated between the neurosensory retina and the vascularized choroid, form part of the Blood–Eye Barrier and are important for homeostasis of the outer retina. These cells are able to produce a variety of cytokines which may play a role in the maintenance of the immunosuppressive milieu inside the Eye and in intraocular inflammatory responses. In the present study, we investigated whether RPE cells secreted the anti-inflammatory cytokine TGF-β2 and the proinflammatory cytokine MCP-1 in a polarized manner. Monolayers of human donor RPE cells were cultured on transwell filters. Secretion of TGF-β2 and MCP-1 at either the apical or basal side of the RPE cell monolayers, that were not treated or stimulated with IL-1β (200 U/ml), was analysed by ELISA. All three cell lines examined had a different TGF-β2 secretion pattern. In two of the three donor RPE cell lines tested, TGF-β2 secretion was polarized, but not in the same direction. TGF-β2 secretion was not up-regulated by stimulation with IL-1β. In contrast, IL-1β strongly induced MCP-1 secretion preferentially into the basal compartment of all RPE monolayers tested. These data indicate that human RPE cells are able to secrete TGF-β2 and MCP-1 in a polarized fashion. Our results suggest that MCP-1 can be secreted by RPE cells in the direction of choroidal vessels during inflammatory responses in the posterior part of the Eye, which may limit damage to the neurosensory retina.
R Frank - One of the best experts on this subject based on the ideXlab platform.
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Neurothelin: amino acid sequence, cell surface dynamics and actin colocalization.
European journal of cell biology, 1995Co-Authors: Burkhard Schlosshauer, H Bauch, R FrankAbstract:Neurothelin is a cell surface protein of chicken endothelial cells at the Blood-brain Barrier and also of pigment epithelial cells forming the Blood-Eye Barrier. Peptide sequencing of affinity-purified neurothelin revealed that it is likely to be identical with the protein called HT7. It belongs to the immunoglobulin superfamily having two extracellular C2-type domains, a membrane spanning region and a cytoplasmic tail. During development neurothelin cell surface localization changed on pigment epithelial cells. In early embryogenesis neurothelin was found preferentially at lateral sites of neighboring epithelial cells, but after hatching, predominantly on basal cell surfaces and on apical microvilli of epithelial cells that contact retinal photoreceptors. Disruption of cell-cell contacts induced a rapid change of neurothelin distribution on the cell surface in vitro as could be shown by confocal laser microscopy. Disintegration of microfilaments by cytochalasin D hampered this specific cell surface rearrangement of neurothelin, whereas depolymerization of microtubules by demecolcine had no effect. In double-labeling experiments neurothelin and F-actin were colocalized. The data suggest that the polarized cell surface distribution of neurothelin is influenced intracellularly by F-actin and extracellularly by cell-cell interactions.
Theresa Bonaventura - One of the best experts on this subject based on the ideXlab platform.
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Breakdown of the Blood-Eye Barrier in choroidal melanoma after proton beam radiotherapy
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Annette Hager, Friederike Meissner, Aline–isabel Riechardt, Theresa Bonaventura, Julia Löwen, Jens Heufelder, Antonia M. JoussenAbstract:Purpose Irradiation of choroidal melanoma is a safe and globe preserving procedure. Chronic inflammatory processes and ischemia are the main reasons for secondary enucleation in the long run. The aim of this study was to determine whether intraocular inflammation and especially inflammatory response after proton beam therapy (PBT) is related to primary tumor characteristics such as height, tumor volume, and initial flare values. Methods Twenty-six patients treated for uveal melanoma using PBT were included. All patients were examined for signs of inflammation using laser flare photometry (LFP). Each examination included assessment of the melanoma and fellow Eye (which acted as the control) and imaging of the melanoma. Results Significant differences of flare values between melanoma Eyes and control group were found both at baseline (median 17.65 ph/ms (min 4, max 37.10), 7.45 ph/ms (min 0.80, max 16.40), respectively) and during follow-up (median 21.45 ph/ms (min 4.5, max 70.90); 6.05 ph/ms (min 2.40, max 16.40), respectively) ( p
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breakdown of the Blood Eye Barrier in choroidal melanoma after proton beam radiotherapy
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Annette Hager, Friederike Meissner, Aline–isabel Riechardt, Theresa Bonaventura, Julia Löwen, Jens Heufelder, Antonia M. JoussenAbstract:PURPOSE Irradiation of choroidal melanoma is a safe and globe preserving procedure. Chronic inflammatory processes and ischemia are the main reasons for secondary enucleation in the long run. The aim of this study was to determine whether intraocular inflammation and especially inflammatory response after proton beam therapy (PBT) is related to primary tumor characteristics such as height, tumor volume, and initial flare values. METHODS Twenty-six patients treated for uveal melanoma using PBT were included. All patients were examined for signs of inflammation using laser flare photometry (LFP). Each examination included assessment of the melanoma and fellow Eye (which acted as the control) and imaging of the melanoma. RESULTS Significant differences of flare values between melanoma Eyes and control group were found both at baseline (median 17.65 ph/ms (min 4, max 37.10), 7.45 ph/ms (min 0.80, max 16.40), respectively) and during follow-up (median 21.45 ph/ms (min 4.5, max 70.90); 6.05 ph/ms (min 2.40, max 16.40), respectively) (p < 0.001, Wilcoxon test). Flare values in melanoma Eyes increased significantly after PBT (p = 0.005, Wilcoxon test) and after a follow-up of 94 days (median, 7-420 days). Flare values of the control group did not change (p = 0.946, Wilcoxon test). The increase of flare values correlated significantly with maximum tumor height and volume (Spearman-Rho 0.633, p = 0.001 and 0.519, p = 0.007, respectively). CONCLUSION LFP has proven to show significantly higher flare values in melanoma Eyes as compared with the control group and provides data on the course of the inflammatory response after treatment. It may ease treatment planning both at baseline and during follow-up.