The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform
Franz L. Dickert - One of the best experts on this subject based on the ideXlab platform.
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Micro-structured interdigital capacitors with synthetic antibody receptors for ABO Blood-Group Typing
Sensors and Actuators B: Chemical, 2017Co-Authors: Adnan Mujahid, Stephan Aigner, Franz L. DickertAbstract:Abstract Interdigital capacitors coated with surface imprinted polymer layers can be designed for ABO-Blood Group Typing. Measurements with 10 mm IDCs in deionized water suggested a change in dielectric properties by erythrocyte adhesion which leads to increase in capacitance. In hypotonic conditions, reduction in resistance was monitored as the erythrocytes interacted with imprinted polymer layers, subsequently the cells burst and the ions were enriched near interdigital electrodes. A clear differentiation among all ABO-Blood Groups was achieved even for A-subGroups, with selectivity factor as high as four i.e. in case of Blood Group B Typing. The basis of these findings are selective interactions between erythrocyte antigens with imprinted sensor coatings. Under isotonic conditions, inclusion of erythrocytes into cavities leads to increase in resistance, because of closed pores in the polymer layers hinder ion diffusion. An enhancement of this sensor signal was observed by a factor of 10 through using smaller IDCs electrode width i.e. 5 μm. Reversibility was improved by using different concentrations of sodium chloride for washing. Optimal frequency was found to be 10 kHz for the measurements to be done. Capacitive measurements confirmed that sensor layers can be reused even after 300 h of operation for Blood Group Typing.
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Blood Group Typing from classical strategies to the application of synthetic antibodies generated by molecular imprinting
Sensors, 2015Co-Authors: Adnan Mujahid, Franz L. DickertAbstract:Blood transfusion requires a mandatory cross-match test to examine the compatibility between donor and recipient Blood Groups. Generally, in all cross-match tests, a specific chemical reaction of antibodies with erythrocyte antigens is carried out to monitor agglutination. Since the visual inspection is no longer useful for obtaining precise quantitative information, therefore there is a wide variety of different technologies reported in the literature to recognize the agglutination reactions. Despite the classical methods, modern biosensors and molecular Blood Typing strategies have also been considered for straightforward, accurate and precise analysis. The interfacial part of a typical sensor device could range from natural antibodies to synthetic receptor materials, as designed by molecular imprinting and which is suitably integrated with the transducer surface. Herein, we present a comprehensive overview of some selected strategies extending from traditional practices to modern procedures in Blood Group Typing, thus to highlight the most promising approach among emerging technologies.
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sensors for healthcare monitoring proteins viruses and Blood Group Typing
2009Co-Authors: Franz L. Dickert, Peter A Lieberzeit, Alexandra Seifner, Romana Schirhagl, Christof JungbauerAbstract:Molecular imprinting strategies could successfully be applied to bioanalytes of different dimensions, ranging from proteins, as insulin, to picornaviruses, Human-Rhino- Virus (HRV) or Foot-Mouth-Disease-Virus (FMDV) to cells, e.g. yeast-cells or even highly flexible erythrocytes. Blood cell surface patterning of pre-polymers could be achieved via coatings with selective recognition properties. Polymer layers coupled with mass-sensitive quartz crystal micro balances (QCMs) lead to a selective sensor system concerning Blood Group Typing. Thus, Blood Groups A1, A2 and B can be distinguished by the imprinted layers acting as synthetic antibodies. Further development of synthetic receptor sites were realized by generating plastic replica of immunoglobulin-Y (IgY), resulting in an antibody-like structure integrated on the polymer surface. For this purpose nano-particles from polymers were imprinted with natural immunoglobulin. After removing the template these patterned particles were pressed in a prepolymer resulting in a synthetic receptor having antibody properties. The sensitivity to the allergen sesame protein was even higher than to the natural analogue.
Adnan Mujahid - One of the best experts on this subject based on the ideXlab platform.
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Micro-structured interdigital capacitors with synthetic antibody receptors for ABO Blood-Group Typing
Sensors and Actuators B: Chemical, 2017Co-Authors: Adnan Mujahid, Stephan Aigner, Franz L. DickertAbstract:Abstract Interdigital capacitors coated with surface imprinted polymer layers can be designed for ABO-Blood Group Typing. Measurements with 10 mm IDCs in deionized water suggested a change in dielectric properties by erythrocyte adhesion which leads to increase in capacitance. In hypotonic conditions, reduction in resistance was monitored as the erythrocytes interacted with imprinted polymer layers, subsequently the cells burst and the ions were enriched near interdigital electrodes. A clear differentiation among all ABO-Blood Groups was achieved even for A-subGroups, with selectivity factor as high as four i.e. in case of Blood Group B Typing. The basis of these findings are selective interactions between erythrocyte antigens with imprinted sensor coatings. Under isotonic conditions, inclusion of erythrocytes into cavities leads to increase in resistance, because of closed pores in the polymer layers hinder ion diffusion. An enhancement of this sensor signal was observed by a factor of 10 through using smaller IDCs electrode width i.e. 5 μm. Reversibility was improved by using different concentrations of sodium chloride for washing. Optimal frequency was found to be 10 kHz for the measurements to be done. Capacitive measurements confirmed that sensor layers can be reused even after 300 h of operation for Blood Group Typing.
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Blood Group Typing from classical strategies to the application of synthetic antibodies generated by molecular imprinting
Sensors, 2015Co-Authors: Adnan Mujahid, Franz L. DickertAbstract:Blood transfusion requires a mandatory cross-match test to examine the compatibility between donor and recipient Blood Groups. Generally, in all cross-match tests, a specific chemical reaction of antibodies with erythrocyte antigens is carried out to monitor agglutination. Since the visual inspection is no longer useful for obtaining precise quantitative information, therefore there is a wide variety of different technologies reported in the literature to recognize the agglutination reactions. Despite the classical methods, modern biosensors and molecular Blood Typing strategies have also been considered for straightforward, accurate and precise analysis. The interfacial part of a typical sensor device could range from natural antibodies to synthetic receptor materials, as designed by molecular imprinting and which is suitably integrated with the transducer surface. Herein, we present a comprehensive overview of some selected strategies extending from traditional practices to modern procedures in Blood Group Typing, thus to highlight the most promising approach among emerging technologies.
Gil Garnier - One of the best experts on this subject based on the ideXlab platform.
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photothermal incubation of red Blood cells by laser for rapid pre transfusion Blood Group Typing
Scientific Reports, 2019Co-Authors: Clare A Manderson, Heather Mcliesh, Rodrigo Curvello, Rico F Tabor, Jim Manolios, Gil GarnierAbstract:Safe Blood transfusion requires compatibility testing of donor and recipient to prevent potentially fatal transfusion reactions. Detection of immunoglobulin G (IgG) antibodies requires incubation at 37 °C, often for up to 15 minutes. Current incubation technology predominantly relies on slow thermal-gradient dependent conduction. Here, we present rapid optical heating via laser, where targeted illumination of a Blood-antibody sample in a diagnostic gel card is converted into heat, via photothermal absorption. Our laser-incubator heats the 75 µL Blood-antibody sample to 37 °C in under 30 seconds. We show that red Blood cells act as photothermal agents under near-infrared laser incubation, triggering rapid antigen-antibody binding. We detect no significant damage to the cells or antibodies for laser incubations of up to fifteen minutes. We demonstrate laser-incubated immunohaematological testing to be both faster and more sensitive than current best practice — with clearly positive results seen from laser incubations of just 40 seconds.
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quantitative Blood Group Typing using surface plasmon resonance
Biosensors and Bioelectronics, 2015Co-Authors: Whui Lyn Then, Marieisabel Aguilar, Gil GarnierAbstract:The accurate and reliable Typing of Blood Groups is essential prior to Blood transfusion. While current Blood Typing methods are well established, results are subjective and heavily reliant on analysis by trained personnel. Techniques for quantifying Blood Group antibody-antigen interactions are also very limited. Many biosensing systems rely on surface plasmon resonance (SPR) detection to quantify biomolecular interactions. While SPR has been widely used for characterizing antibody-antigen interactions, measuring antibody interactions with whole cells is significantly less common. Previous studies utilized SPR for Blood Group antigen detection, however, showed poor regeneration causing loss of functionality after a single use. In this study, a fully regenerable, multi-functional platform for quantitative Blood Group Typing via SPR detection is achieved by immobilizing anti-human IgG antibody to the sensor surface, which binds to the Fc region of human IgG antibodies. The surface becomes an interchangeable platform capable of quantifying the Blood Group interactions between red Blood cells (RBCs) and IgG antibodies. As with indirect antiglobulin tests (IAT), which use IgG antibodies for detection, IgG antibodies are initially incubated with RBCs. This facilitates binding to the immobilized monolayer and allows for quantitative Blood Group detection. Using the D-antigen as an example, a clear distinction between positive (>500 RU) and negative (<100 RU) RBCs is achieved using anti-D IgG. Complete regeneration of the anti-human IgG surface is also successful, showing negligible degradation of the surface after more than 100 regenerations. This novel approach is validated with human-sourced whole Blood samples to demonstrate an interesting alternative for quantitative Blood Grouping using SPR analysis.
Mongkol Kunakorn - One of the best experts on this subject based on the ideXlab platform.
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microfluidic pmma based microarray sensor chip with imaging analysis for abo and rhd Blood Group Typing
Vox Sanguinis, 2016Co-Authors: Chinnawut Pipatpanukul, Apirom Vongsakulyanon, Pimpun Kitpoka, Toemsak Srikhirin, Ratthasart Amarit, Armote Somboonkaew, Boonsong Sutapun, Mongkol KunakornAbstract:Background and Objectives Solid phase microarrays have been described for use in Blood Typing; red Blood cells (RBCs) captured on immobilized antibodies were detected using surface plasmon resonance or fluorescence. We present antibody microarray on Poly (methylmethacrylate) (PMMA) surface coupled with microfluidic system for ABO and RhD Blood Typing. After immobilized by antigen–antibody interaction, the RBCs were detected by image recognition. Materials and Methods The sensor surface was produced from grafted aminopropyltriethoxysilane (APTES) on photochemical modified PMMA surface by UV irradiation and subsequently reacted with glutaraldehyde cross-linking. The amine Group of monoclonal antibody of anti-A, anti-B and anti-D was reacted with an aldehyde Group on the glutaraldehyde modified surface, forming an imine linkage. RBCs were captured by the coated antibody via antigen–antibody interaction, and Blood Grouping was determined by microarray image cell counting. Results Suitable condition for RBC detection was 10% RBC concentration at 10 μl/min flow rate. This setting eliminated non-specific RBC binding resulting in correct Blood Groups identification of all 136 samples tested. The platform showed good reproducibility with coefficient of variation of 2·17%, 3·62% and 2·51% for anti-A, anti-B and anti-D respectively. The antibody-coated surface can be stabilized by stabilizer coating and stored for long-term use. Conclusion The PMMA array chip demonstrated its good accuracy and precision in rapid Blood Group testing. For its high throughput, the method has potential for use in large Blood donation centre.
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miltenberger Blood Group Typing by real time polymerase chain reaction qpcr melting curve analysis in thai population
Transfusion Medicine, 2015Co-Authors: Apirom Vongsakulyanon, Pimpun Kitpoka, Mongkol Kunakorn, Toemsak SrikhirinAbstract:SUMMARY Objectives To develop reliable and convenient methods for Miltenberger (Mia) Blood Group Typing. Aim To apply real-time polymerase chain reaction (qPCR) melting curve analysis to Mia Blood Group Typing. Background The Mia Blood Group is the collective set of glycophorin hybrids in the MNS Blood Group system. Mi(a+) Blood is common among East Asians and is also found in the Thai population. Incompatible Mia Blood transfusions pose the risk of life-threatening haemolysis; therefore, Mia Blood Group Typing is necessary in ethnicities where the Mia Blood Group is prevalent. Methods/Materials One hundred and forty-three Blood samples from Thai Blood donors were used in the study. The samples included 50 Mi(a+) samples and 93 Mi(a−) samples, which were defined by serology. The samples were typed by Mia Typing qPCR, and 50 Mi(a+) samples were sequenced to identify the Mia subtypes. Mia subTyping qPCR was performed to define GP.Mur. Both Mia Typing and Mia subTyping were tested on a conventional PCR platform. Results The results of Mia Typing qPCR were all concordant with serology. Sequencing of the 50 Mi(a+) samples revealed 47 GP.Mur samples and 3 GP.Hop or Bun samples. Mia subTyping qPCR was the supplementary test used to further define GP.Mur from other Mia subtypes. Both Mia Typing and Mia subTyping performed well using a conventional PCR platform. Conclusion Mia Typing qPCR correctly identified Mia Blood Groups in a Thai population with the feasibility of Mia subtype discrimination, and Mia subTyping qPCR was able to further define GP.Mur from other Mia subtypes.
Richard A. Doherty - One of the best experts on this subject based on the ideXlab platform.
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Fetal ABO Blood Group Typing using amniotic fluid
Clinical Genetics, 2008Co-Authors: Doris Teichler‐zallen, Richard A. DohertyAbstract:Amniotic fluids (AF) obtained by second trimester amniocentesis were examined using a hemagglutination-inhibition test. In the anti-A and anti-B dilution series three different patterns of agglutination were found. One pattern, indicating the presence of high levels of antigen, is typical of secretors (Se/-) of the Blood Group antigen. The second pattern is indicative of total absence of soluble antigen and is produced in secretor (Se/-) and non-secretor (se/se) fetuses lacking the IA or IB allele at the ABO locus. The third pattern shows the presence of trace amounts of soluble antigen, about one-tenth of that found in secretors, and it appears in non-secretor fetuses bearing the corresponding ABO allele. These trace quantities, if a constant feature of AFs from these non-secretor fetuses, could allow Blood Group Typing information to be obtained on all non-secretor as well as secretor fetuses. A total of 157 amniotic fluid samples were surveyed in this and a previous study. Of these, 31 were from non-secretor fetuses. Blood Group type for the 31 non-secretor fetuses was predicted (“blindly”) on the basis of the presence or absence of trace amounts of soluble antigen. These predictions were verified by standard ABO Typing of the infants afterbirth. In each case the (“blind”) Typing result agreed with the predictions made from the titration curves. This agreement establishes that the production of trace amounts of soluble antigen(s) is a consistent feature of non-secretors with the IA and/or IB allele. The soluble Blood Group substances are of fetal origin and are independent of maternal Blood type and secretor status. Type O non-secretors produce no soluble A or B antigen and also lack detectable soluble H antigen. Thus it appears that the nature of the detectable amounts of soluble Blood Group antigen(s) present in second-trimester AF can be used to accurately assess the ABO Blood type of all fetuses.