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Michael R. Strand - One of the best experts on this subject based on the ideXlab platform.

  • Whole Blood and Blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes
    Parasites & Vectors, 2021
    Co-Authors: Ruby E. Harrison, Mark R. Brown, Michael R. Strand
    Abstract:

    Background Most female mosquitoes are anautogenous and must Blood feed on a vertebrate host to produce eggs. Prior studies show that the number of eggs females lay per clutch correlates with the volume of Blood ingested and that protein is the most important macronutrient for egg formation. In contrast, how whole Blood, Blood fractions and specific Blood Proteins from different vertebrates affect egg formation is less clear. Since egg formation is best understood in Aedes aegypti, we examined how Blood and Blood components from different vertebrates affect this species and two others: the malaria vector Anopheles gambiae and arbovirus vector Culex quinquefasciatus . Methods Adult female mosquitoes were fed Blood, Blood fractions and purified major Blood Proteins from different vertebrate hosts. Markers of reproductive response including ovary ecdysteroidogenesis, yolk deposition into oocytes and number of mature eggs produced were measured. Results Ae. aegypti, An. gambiae and C. quinquefasciatus responded differently to meals of whole Blood, plasma or Blood cells from human, rat, chicken and turkey hosts. We observed more similarities between the anthropophiles Ae. aegypti and An. gambiae than the ornithophile C. quinquefasciatus. Focusing on Ae. aegypti, the major plasma-derived Proteins (serum albumin, fibrinogen and globulins) differentially stimulated egg formation as a function of vertebrate host source. The major Blood cell protein, hemoglobin, stimulated yolk deposition when from pigs but not humans, cows or sheep. Serum albumins from different vertebrates also variably affected egg formation. Bovine serum albumin (BSA) stimulated ovary ecdysteroidogenesis, but more weakly induced digestive enzyme activities than whole Blood. In contrast, BSA-derived peptides and free amino acids had no stimulatory effects on ecdysteroidogenesis or yolk deposition into oocytes. Conclusions Whole Blood, Blood fractions and specific Blood Proteins supported egg formation in three species of anautogenous mosquitoes but specific responses varied with the vertebrate source of the Blood components tested.

  • Whole Blood and Blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes.
    Parasites & vectors, 2021
    Co-Authors: Ruby E. Harrison, Mark R. Brown, Michael R. Strand
    Abstract:

    Most female mosquitoes are anautogenous and must Blood feed on a vertebrate host to produce eggs. Prior studies show that the number of eggs females lay per clutch correlates with the volume of Blood ingested and that protein is the most important macronutrient for egg formation. In contrast, how whole Blood, Blood fractions and specific Blood Proteins from different vertebrates affect egg formation is less clear. Since egg formation is best understood in Aedes aegypti, we examined how Blood and Blood components from different vertebrates affect this species and two others: the malaria vector Anopheles gambiae and arbovirus vector Culex quinquefasciatus. Adult female mosquitoes were fed Blood, Blood fractions and purified major Blood Proteins from different vertebrate hosts. Markers of reproductive response including ovary ecdysteroidogenesis, yolk deposition into oocytes and number of mature eggs produced were measured. Ae. aegypti, An. gambiae and C. quinquefasciatus responded differently to meals of whole Blood, plasma or Blood cells from human, rat, chicken and turkey hosts. We observed more similarities between the anthropophiles Ae. aegypti and An. gambiae than the ornithophile C. quinquefasciatus. Focusing on Ae. aegypti, the major plasma-derived Proteins (serum albumin, fibrinogen and globulins) differentially stimulated egg formation as a function of vertebrate host source. The major Blood cell protein, hemoglobin, stimulated yolk deposition when from pigs but not humans, cows or sheep. Serum albumins from different vertebrates also variably affected egg formation. Bovine serum albumin (BSA) stimulated ovary ecdysteroidogenesis, but more weakly induced digestive enzyme activities than whole Blood. In contrast, BSA-derived peptides and free amino acids had no stimulatory effects on ecdysteroidogenesis or yolk deposition into oocytes. Whole Blood, Blood fractions and specific Blood Proteins supported egg formation in three species of anautogenous mosquitoes but specific responses varied with the vertebrate source of the Blood components tested.

Ruby E. Harrison - One of the best experts on this subject based on the ideXlab platform.

  • Whole Blood and Blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes
    Parasites & Vectors, 2021
    Co-Authors: Ruby E. Harrison, Mark R. Brown, Michael R. Strand
    Abstract:

    Background Most female mosquitoes are anautogenous and must Blood feed on a vertebrate host to produce eggs. Prior studies show that the number of eggs females lay per clutch correlates with the volume of Blood ingested and that protein is the most important macronutrient for egg formation. In contrast, how whole Blood, Blood fractions and specific Blood Proteins from different vertebrates affect egg formation is less clear. Since egg formation is best understood in Aedes aegypti, we examined how Blood and Blood components from different vertebrates affect this species and two others: the malaria vector Anopheles gambiae and arbovirus vector Culex quinquefasciatus . Methods Adult female mosquitoes were fed Blood, Blood fractions and purified major Blood Proteins from different vertebrate hosts. Markers of reproductive response including ovary ecdysteroidogenesis, yolk deposition into oocytes and number of mature eggs produced were measured. Results Ae. aegypti, An. gambiae and C. quinquefasciatus responded differently to meals of whole Blood, plasma or Blood cells from human, rat, chicken and turkey hosts. We observed more similarities between the anthropophiles Ae. aegypti and An. gambiae than the ornithophile C. quinquefasciatus. Focusing on Ae. aegypti, the major plasma-derived Proteins (serum albumin, fibrinogen and globulins) differentially stimulated egg formation as a function of vertebrate host source. The major Blood cell protein, hemoglobin, stimulated yolk deposition when from pigs but not humans, cows or sheep. Serum albumins from different vertebrates also variably affected egg formation. Bovine serum albumin (BSA) stimulated ovary ecdysteroidogenesis, but more weakly induced digestive enzyme activities than whole Blood. In contrast, BSA-derived peptides and free amino acids had no stimulatory effects on ecdysteroidogenesis or yolk deposition into oocytes. Conclusions Whole Blood, Blood fractions and specific Blood Proteins supported egg formation in three species of anautogenous mosquitoes but specific responses varied with the vertebrate source of the Blood components tested.

  • Whole Blood and Blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes.
    Parasites & vectors, 2021
    Co-Authors: Ruby E. Harrison, Mark R. Brown, Michael R. Strand
    Abstract:

    Most female mosquitoes are anautogenous and must Blood feed on a vertebrate host to produce eggs. Prior studies show that the number of eggs females lay per clutch correlates with the volume of Blood ingested and that protein is the most important macronutrient for egg formation. In contrast, how whole Blood, Blood fractions and specific Blood Proteins from different vertebrates affect egg formation is less clear. Since egg formation is best understood in Aedes aegypti, we examined how Blood and Blood components from different vertebrates affect this species and two others: the malaria vector Anopheles gambiae and arbovirus vector Culex quinquefasciatus. Adult female mosquitoes were fed Blood, Blood fractions and purified major Blood Proteins from different vertebrate hosts. Markers of reproductive response including ovary ecdysteroidogenesis, yolk deposition into oocytes and number of mature eggs produced were measured. Ae. aegypti, An. gambiae and C. quinquefasciatus responded differently to meals of whole Blood, plasma or Blood cells from human, rat, chicken and turkey hosts. We observed more similarities between the anthropophiles Ae. aegypti and An. gambiae than the ornithophile C. quinquefasciatus. Focusing on Ae. aegypti, the major plasma-derived Proteins (serum albumin, fibrinogen and globulins) differentially stimulated egg formation as a function of vertebrate host source. The major Blood cell protein, hemoglobin, stimulated yolk deposition when from pigs but not humans, cows or sheep. Serum albumins from different vertebrates also variably affected egg formation. Bovine serum albumin (BSA) stimulated ovary ecdysteroidogenesis, but more weakly induced digestive enzyme activities than whole Blood. In contrast, BSA-derived peptides and free amino acids had no stimulatory effects on ecdysteroidogenesis or yolk deposition into oocytes. Whole Blood, Blood fractions and specific Blood Proteins supported egg formation in three species of anautogenous mosquitoes but specific responses varied with the vertebrate source of the Blood components tested.

Brent D. Cameron - One of the best experts on this subject based on the ideXlab platform.

  • Aptamer-based surface plasmon resonance sensing of glycated human Blood Proteins
    Plasmonics in Biology and Medicine X, 2013
    Co-Authors: Nathan G. F. Reaver, Rui Zheng, Dong-shik Kim, Brent D. Cameron
    Abstract:

    The concentration ratio of glycated to non-glycated forms of various Blood Proteins can be used as a diagnostic measure in diabetes to determine a history of glycemic compliance. Depending on a protein’s half-life in Blood, compliance can be assessed from a few days to several months in the past, which can then be used to provide additional therapeutic guidance. Current glycated protein detection methods are limited in their ability to measure multiple Proteins, and are susceptible to interference from other Blood pathologies. In this study, we developed and characterized DNA aptamers for use in Surface Plasmon Resonance (SPR) sensors to assess the Blood protein hemoglobin. The aptamers were developed by way of a modified Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process which selects DNA sequences that have a high binding affinity to a specific protein. DNA products resulting from this process are sequenced and identified aptamers are then synthesized. The SELEX process was performed to produce aptamers for a glycated form of hemoglobin. Equilibrium dissociation constants for the binding of the identified aptamer to glycated hemoglobin, hemoglobin, and fibrinogen were calculated from fitted Langmuir isotherms obtained through SPR. These constants were determined to be 94 nM, 147 nM, and 244 nM respectively. This aptamer can potentially be used to create a SPR aptamer based biosensor for detection of glycated hemoglobin, a technology that has the potential to deliver low-cost and immediate glycemic compliance assessment in either a clinical or home setting.

  • Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for Blood protein detection.
    Biomedical optics express, 2011
    Co-Authors: Rui Zheng, Dong-shik Kim, Byung-wook Park, Brent D. Cameron
    Abstract:

    This paper presents a generally applicable approach for the highly specific detection of Blood Proteins. Thrombin and thrombin-binding aptamers are chosen for demonstration purposes. The sensor was prepared by immobilizing amine-terminated aptamers onto a gold modified surface using a two-step self-assembled monolayer (SAM) immobilization technique and the physical detection is performed using Surface Plasmon Resonance (SPR). The developed sensor has an optimal detectable range of 5-1000 nM and the results show the sensor has good reversibility, sensitivity and selectivity. Furthermore, the sensor shows the potential of being improved and standardized for direct detection of other Blood Proteins for clinical applications.

Mark R. Brown - One of the best experts on this subject based on the ideXlab platform.

  • Whole Blood and Blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes
    Parasites & Vectors, 2021
    Co-Authors: Ruby E. Harrison, Mark R. Brown, Michael R. Strand
    Abstract:

    Background Most female mosquitoes are anautogenous and must Blood feed on a vertebrate host to produce eggs. Prior studies show that the number of eggs females lay per clutch correlates with the volume of Blood ingested and that protein is the most important macronutrient for egg formation. In contrast, how whole Blood, Blood fractions and specific Blood Proteins from different vertebrates affect egg formation is less clear. Since egg formation is best understood in Aedes aegypti, we examined how Blood and Blood components from different vertebrates affect this species and two others: the malaria vector Anopheles gambiae and arbovirus vector Culex quinquefasciatus . Methods Adult female mosquitoes were fed Blood, Blood fractions and purified major Blood Proteins from different vertebrate hosts. Markers of reproductive response including ovary ecdysteroidogenesis, yolk deposition into oocytes and number of mature eggs produced were measured. Results Ae. aegypti, An. gambiae and C. quinquefasciatus responded differently to meals of whole Blood, plasma or Blood cells from human, rat, chicken and turkey hosts. We observed more similarities between the anthropophiles Ae. aegypti and An. gambiae than the ornithophile C. quinquefasciatus. Focusing on Ae. aegypti, the major plasma-derived Proteins (serum albumin, fibrinogen and globulins) differentially stimulated egg formation as a function of vertebrate host source. The major Blood cell protein, hemoglobin, stimulated yolk deposition when from pigs but not humans, cows or sheep. Serum albumins from different vertebrates also variably affected egg formation. Bovine serum albumin (BSA) stimulated ovary ecdysteroidogenesis, but more weakly induced digestive enzyme activities than whole Blood. In contrast, BSA-derived peptides and free amino acids had no stimulatory effects on ecdysteroidogenesis or yolk deposition into oocytes. Conclusions Whole Blood, Blood fractions and specific Blood Proteins supported egg formation in three species of anautogenous mosquitoes but specific responses varied with the vertebrate source of the Blood components tested.

  • Whole Blood and Blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes.
    Parasites & vectors, 2021
    Co-Authors: Ruby E. Harrison, Mark R. Brown, Michael R. Strand
    Abstract:

    Most female mosquitoes are anautogenous and must Blood feed on a vertebrate host to produce eggs. Prior studies show that the number of eggs females lay per clutch correlates with the volume of Blood ingested and that protein is the most important macronutrient for egg formation. In contrast, how whole Blood, Blood fractions and specific Blood Proteins from different vertebrates affect egg formation is less clear. Since egg formation is best understood in Aedes aegypti, we examined how Blood and Blood components from different vertebrates affect this species and two others: the malaria vector Anopheles gambiae and arbovirus vector Culex quinquefasciatus. Adult female mosquitoes were fed Blood, Blood fractions and purified major Blood Proteins from different vertebrate hosts. Markers of reproductive response including ovary ecdysteroidogenesis, yolk deposition into oocytes and number of mature eggs produced were measured. Ae. aegypti, An. gambiae and C. quinquefasciatus responded differently to meals of whole Blood, plasma or Blood cells from human, rat, chicken and turkey hosts. We observed more similarities between the anthropophiles Ae. aegypti and An. gambiae than the ornithophile C. quinquefasciatus. Focusing on Ae. aegypti, the major plasma-derived Proteins (serum albumin, fibrinogen and globulins) differentially stimulated egg formation as a function of vertebrate host source. The major Blood cell protein, hemoglobin, stimulated yolk deposition when from pigs but not humans, cows or sheep. Serum albumins from different vertebrates also variably affected egg formation. Bovine serum albumin (BSA) stimulated ovary ecdysteroidogenesis, but more weakly induced digestive enzyme activities than whole Blood. In contrast, BSA-derived peptides and free amino acids had no stimulatory effects on ecdysteroidogenesis or yolk deposition into oocytes. Whole Blood, Blood fractions and specific Blood Proteins supported egg formation in three species of anautogenous mosquitoes but specific responses varied with the vertebrate source of the Blood components tested.

Ui-rak Kim - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of Blood Proteins on protein arrays with a spectral surface plasmon resonance biosensor
    Current Applied Physics, 2007
    Co-Authors: Jong Seol Yuk, Myung-jae Lee, Ui-rak Kim
    Abstract:

    Abstract We investigated whether Blood Proteins can be analyzed on protein arrays with a spectral surface plasmon resonance (SPR) biosensor. We modified gold arrays with a mixture of mercaptoundecanoic acid and mercaptohexanol, and immobilized Blood Proteins-hemoglobin (Hb) and haptoglobin (Hp), or their antibodies. We analyzed interactions of Hb with Hp on protein arrays by measuring the shift of resonance wavelength, and a significant interaction was observed when Hp was used as a ligand. Then, we fabricated antibody arrays with antibodies against Hb and Hp, and analyzed binding of Blood Proteins onto the arrays. Anti-Hb showed a specific interaction with Hb on the antibody arrays. Interaction of anti-Hp with Hp was specific when Hp was used as capture molecules. The shift of resonance wavelength caused by the interaction of Blood Proteins was explained by changes of refractive index on the gold surfaces of protein arrays. The present work suggests that spectral SPR biosensors can be used as a useful tool for the analysis of Blood Proteins on protein arrays.