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Sathishkumar Ramalingam - One of the best experts on this subject based on the ideXlab platform.
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dna barcode its effectively distinguishes the medicinal plant Boerhavia diffusa from its adulterants
Genomics Proteomics & Bioinformatics, 2012Co-Authors: Dhivya Selvaraj, Dhivya Shanmughanandhan, Rajeev Kumar Sarma, Jijo C Joseph, Ramachandran V Srinivasan, Sathishkumar RamalingamAbstract:Boerhavia diffusa (B. diffusa), also known as Punarnava, is an indigenous plant in India and an important component in traditional Indian medicine. The accurate identification and collection of this medicinal herb is vital to enhance the drug's efficacy and biosafety. In this study, a DNA barcoding technique has been applied to identify and distinguish B. diffusa from its closely-related species. The phylogenetic analysis was carried out for the four species of Boerhavia using barcode candidates including nuclear ribosomal DNA regions ITS, ITS1, ITS2 and the chloroplast plastid gene psbA-trnH. Sequence alignment revealed 26% polymorphic sites in ITS, 30% in ITS1, 16% in ITS2 and 6% in psbA-trnH, respectively. Additionally, a phylogenetic tree was constructed for 15 species using ITS sequences which clearly distinguished B. diffusa from the other species. The ITS1 demonstrates a higher transition/transversion ratio, percentage of variation and pairwise distance which differentiate B. diffusa from other species of Boerhavia. Our study revealed that ITS and ITS1 could be used as potential candidate regions for identifying B. diffusa and for authenticating its herbal products.
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application note dna barcode its effectively distinguishes the medicinal plant Boerhavia diffusa from its adulterants
2012Co-Authors: Dhivya Selvaraj, Dhivya Shanmughanandhan, Rajeev Kumar Sarma, Jijo C Joseph, Ramachandran V Srinivasan, Sathishkumar RamalingamAbstract:Boerhavia diffusa (B. diffusa), also known as Punarnava, is an indigenous plant in India and an important component in traditional Indian medicine. The accurate identification and collection of this medicinal herb is vital to enhance the drug’s efficacy and biosafety. In this study, a DNA barcoding technique has been applied to identify and distinguish B. diffusa from its closely-related species. The phylogenetic analysis was carried out for the four species of Boerhavia using barcode candidates including nuclear ribosomal DNA regions ITS, ITS1, ITS2 and the chloroplast plastid gene psbA-trnH. Sequence alignment revealed 26% polymorphic sites in ITS, 30% in ITS1, 16% in ITS2 and 6% in psbA-trnH, respectively. Additionally, a phylogenetic tree was constructed for 15 species using ITS sequences which clearly distinguished B. diffusa from the other species. The ITS1 demonstrates a higher transition/transversion ratio, percentage of variation and pairwise distance which differentiate B. diffusa from other species of Boerhavia. Our study revealed that ITS and ITS1 could be used as potential candidate regions for identifying B. diffusa and for authenticating its herbal products.
R.g.s. Wijesekara - One of the best experts on this subject based on the ideXlab platform.
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Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay.
Data in brief, 2018Co-Authors: Kotaro Hara, Takao Someya, Katsura Sano, Yoshimasa Sagane, Toshihiro Watanabe, R.g.s. WijesekaraAbstract:This article describes free radical-scavenging activities of extracts of several plants harvested in Sri Lanka through the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. These plants have traditionally been used in the indigenous systems of medicine in Sri Lanka, such as Ayurveda, as described below. (English name, "local name in Sri Lanka," (scientific name)). bougainvillea plant, "bouganvilla," (Bougainvillea grabla), purple fruited pea eggplant,"welthibbatu," (Solanum trilobatum) [1], country borage plant, "kapparawalliya," (Plectranthus amboinicus) [2], malabar nut plant, "adhatoda," (Justicia adhatoda) [3], long pepper plant,"thippili," (Piper longum) [4], holy basil plant, "maduruthala," (Ocimum tenuiflorum) [5], air plant, "akkapana," (Kalanchoe pinnata) [6], plumed cockscomb plant, "kiri-henda," (Celosia argentea) [7], neem plant,"kohomba," (Azadirachta indica) [8], balipoovu plant, "polpala," (Aerva lanata) [9], balloon-vine plant, "wel penera," (Cardiospermum halicacabum) [10], emblic myrobalan plant, "nelli," (Phyllanthus emblica) [11], indian copperleaf plant, "kuppameniya," (Acalypha indica) [12], spreading hogweed plant, "pita sudu sarana," (Boerhavia diffusa) [13], curry leaf plant, "karapincha," (Murraya koenigii) [14], indian pennywort plant, "gotukola," (Centera asiatica) [15], jewish plum plant, "ambarella,"(Spondias dulcis) [16].
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Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay
Elsevier, 2018Co-Authors: Kotaro Hara, Takao Someya, Katsura Sano, Yoshimasa Sagane, Toshihiro Watanabe, R.g.s. WijesekaraAbstract:This article describes free radical-scavenging activities of extracts of several plants harvested in Sri Lanka through the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. These plants have traditionally been used in the indigenous systems of medicine in Sri Lanka, such as Ayurveda, as described below. (English name, “local name in Sri Lanka,” (scientific name)).bougainvillea plant, “bouganvilla,” (Bougainvillea grabla), purple fruited pea eggplant,”welthibbatu,” (Solanum trilobatum) [1], country borage plant, “kapparawalliya,” (Plectranthus amboinicus) [2], malabar nut plant, “adhatoda,” (Justicia adhatoda) [3], long pepper plant,”thippili,” (Piper longum) [4], holy basil plant, “maduruthala,” (Ocimum tenuiflorum) [5], air plant, “akkapana,” (Kalanchoe pinnata) [6], plumed cockscomb plant, “kiri-henda,” (Celosia argentea) [7], neem plant,”kohomba,” (Azadirachta indica) [8], balipoovu plant, “polpala,” (Aerva lanata) [9], balloon-vine plant, “wel penera,” (Cardiospermum halicacabum) [10], emblic myrobalan plant, “nelli,” (Phyllanthus emblica) [11], indian copperleaf plant, “kuppameniya,” (Acalypha indica) [12], spreading hogweed plant, “pita sudu sarana,” (Boerhavia diffusa) [13], curry leaf plant, “karapincha,” (Murraya koenigii) [14], indian pennywort plant, “gotukola,” (Centera asiatica) [15], jewish plum plant, “ambarella,”(Spondias dulcis) [16]. Keywords: Antioxidative activity, DPPH radical-scavenging assay, Traditional plant, Medical her
Debabrata Sircar - One of the best experts on this subject based on the ideXlab platform.
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metabolite profiling and wound healing activity of Boerhavia diffusa leaf extracts using in vitro and in vivo models
Journal of Traditional and Complementary Medicine, 2020Co-Authors: Kriti Juneja, Rutusmita Mishra, Samrat Chauhan, Sumeet Gupta, Partha Pratim Roy, Debabrata SircarAbstract:Abstract Boerhavia diffusa is a perennial herb belonging to the Nyctaginaceae family. This plant has been widely used in Indian traditional medicinal system to cure several human ailments. However, traditional use of this plant in the treatment and management of wounds has not been validated by any comprehensive scientific study. The present study was aimed to explore the in vitro and in vivo wound healing potential of methanol extract (ME) and chloroform extract (CE) from B. diffusa leaf and subsequent identification of the bioactive metabolites, which might be responsible for enhancement of wound healing property of the extracts. The study included in vitro cell viability and wound scratch assays as well as in vivo excision wound assays in rat models. Both ME and CE were analysed for their antioxidant properties and phenolics content. The gas chromatography-mass spectrometry analyses were performed for identification of bioactive metabolites present in the ME and CE. ME of B. diffusa leaf significantly enhanced viability and migration of human keratinocyte cells (HaCaT) as compared to the untreated and CE-treated groups. The topical application of ME of B. diffusa leaf in excision wound model significantly decreased the wound area by the 14th day (91%) as compared to control (22%) (p
Nicholas Ekow Thomford - One of the best experts on this subject based on the ideXlab platform.
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bush mint hyptis suaveolens and spreading hogweed Boerhavia diffusa medicinal plant extracts differentially affect activities of cyp1a2 cyp2d6 and cyp3a4 enzymes
Journal of Ethnopharmacology, 2018Co-Authors: Nicholas Ekow Thomford, Kevin Dzobo, Faustina Adu, Shadreck Chirikure, Ambroise Wonkam, Collet DandaraAbstract:Abstract Ethno-pharmacological relevance Hyptis suaveolens (L) Poit and Boerhavia diffusa Linn are medicinal herbal plants commonly found in the tropics and sub-tropics. They are used to treat various conditions among them boils, dyslipidaemia, eczema, malaria, jaundice and gonorrhoea. Thus, the herbal medicinal extracts are now found as part of some commercial herbal formulations. There has not been adequate characterization of these medicinal herbs on their effects on drug metabolising enzymes. Aim of the study To investigate the effects of extracts of Hyptis suaveolens (HS) and Boerhavia diffusa (BD) on activity of drug metabolising enzymes, CYP1A2, CYP2D6 and CYP3A4, as well predict their potential for herb-drug interaction. A secondary aim was to identify constituent compounds such as polyphenolics, in the crude extract preparations of Hyptis suaveolens and Boerhavia diffusa and measure them for activity. Materials and methods CYP450 inhibition assays using recombinant CYP450 (rCYP) and fluorescence screening employing individual isozymes (CYP1A2, CYP2D6 and CYP3A4) were used to determine reversible- and time-dependent inhibition (TDI) profiles of extracts of Hyptis suaveolens and Boerhavia diffusa. Inhibition kinetic parameters, Ki and Kinact were also estimated. UPLC-MS employing a Synapt G2 (ESI negative) coupled to a PDA detector was used to identify polyphenolic compounds in crude extracts of Hyptis suaveolens and Boerhavia diffusa. Results The inhibitory potency of Hyptis suaveolens and Boerhavia diffusa extracts varied among the different enzymes, with CYP1A2 (3.68 ± 0.10 µg/mL) being the least inhibited by HS compared to CYP2D6 (1.39 ± 0.01 µg/mL) and CYP3A4 (2.36 ± 0.57 µg/mL). BD was most potent on CYP3A4 (7.36 ± 0.94 µg/mL) compared to both CYP2D6 (17.79 ± 1.02 µg/mL) and CYP1A2 (9.48 ± 0.78 µg/mL). Extracts of Hyptis suaveolens and Boerhavia diffusa exhibited TDIs on all CYPs. The most prominent phenolic candidates identified in both medicinal herbs using UPLC-MS analysis included caffeic acid, rutin, quercetin, citric acid, ferulic acid and gluconic acid. These phenolic compounds are thought to potentially give HS and BD their therapeutic effects and inhibitory characteristics affecting CYP450 activities. In vivo predictions showed the potential for HS and BD extracts to cause significant interactions if co-administered with other medications. Conclusions The study reveals that crude aqueous extracts of HS and BD potentially inhibit drug metabolising isozymes CYP1A2, CYP2D6 and CYP3A4 in a reversible and time-dependent manner. Thus care should be taken when these extracts are co-administered with drugs that are substrates of CYP1A2, CYP2D6 and CYP3A4.
Dhivya Selvaraj - One of the best experts on this subject based on the ideXlab platform.
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dna barcode its effectively distinguishes the medicinal plant Boerhavia diffusa from its adulterants
Genomics Proteomics & Bioinformatics, 2012Co-Authors: Dhivya Selvaraj, Dhivya Shanmughanandhan, Rajeev Kumar Sarma, Jijo C Joseph, Ramachandran V Srinivasan, Sathishkumar RamalingamAbstract:Boerhavia diffusa (B. diffusa), also known as Punarnava, is an indigenous plant in India and an important component in traditional Indian medicine. The accurate identification and collection of this medicinal herb is vital to enhance the drug's efficacy and biosafety. In this study, a DNA barcoding technique has been applied to identify and distinguish B. diffusa from its closely-related species. The phylogenetic analysis was carried out for the four species of Boerhavia using barcode candidates including nuclear ribosomal DNA regions ITS, ITS1, ITS2 and the chloroplast plastid gene psbA-trnH. Sequence alignment revealed 26% polymorphic sites in ITS, 30% in ITS1, 16% in ITS2 and 6% in psbA-trnH, respectively. Additionally, a phylogenetic tree was constructed for 15 species using ITS sequences which clearly distinguished B. diffusa from the other species. The ITS1 demonstrates a higher transition/transversion ratio, percentage of variation and pairwise distance which differentiate B. diffusa from other species of Boerhavia. Our study revealed that ITS and ITS1 could be used as potential candidate regions for identifying B. diffusa and for authenticating its herbal products.
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application note dna barcode its effectively distinguishes the medicinal plant Boerhavia diffusa from its adulterants
2012Co-Authors: Dhivya Selvaraj, Dhivya Shanmughanandhan, Rajeev Kumar Sarma, Jijo C Joseph, Ramachandran V Srinivasan, Sathishkumar RamalingamAbstract:Boerhavia diffusa (B. diffusa), also known as Punarnava, is an indigenous plant in India and an important component in traditional Indian medicine. The accurate identification and collection of this medicinal herb is vital to enhance the drug’s efficacy and biosafety. In this study, a DNA barcoding technique has been applied to identify and distinguish B. diffusa from its closely-related species. The phylogenetic analysis was carried out for the four species of Boerhavia using barcode candidates including nuclear ribosomal DNA regions ITS, ITS1, ITS2 and the chloroplast plastid gene psbA-trnH. Sequence alignment revealed 26% polymorphic sites in ITS, 30% in ITS1, 16% in ITS2 and 6% in psbA-trnH, respectively. Additionally, a phylogenetic tree was constructed for 15 species using ITS sequences which clearly distinguished B. diffusa from the other species. The ITS1 demonstrates a higher transition/transversion ratio, percentage of variation and pairwise distance which differentiate B. diffusa from other species of Boerhavia. Our study revealed that ITS and ITS1 could be used as potential candidate regions for identifying B. diffusa and for authenticating its herbal products.