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Bone Tissue Formation

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Meidong Lang – One of the best experts on this subject based on the ideXlab platform.

  • in situ controlled release of rhbmp 2 in gelatin coated 3d porous poly e caprolactone scaffolds for homogeneous Bone Tissue Formation
    Biomacromolecules, 2014
    Co-Authors: Qingchun Zhang, Yan Zhang, Zhaoyang Ye, Meidong Lang

    Abstract:

    In Tissue engineering, incorporation of Bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate Bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable Bone Formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(e-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better Bone format…

  • In situ controlled release of rhBMP-2 in gelatin-coated 3D porous poly(ε-caprolactone) scaffolds for homogeneous Bone Tissue Formation
    Biomacromolecules, 2014
    Co-Authors: Qingchun Zhang, Ke Tan, Yan Zhang, Zhaoyang Ye, Wen-song Tan, Meidong Lang

    Abstract:

    In Tissue engineering, incorporation of Bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate Bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable Bone Formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(ε-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better Bone Formation was observed in gelatin/rhBMP-2-coated scaffolds. Specifically, the spatial distribution of newly formed Bone was more uniform in gelatin-coated scaffolds than in uncoated scaffolds, which displayed preferential Bone Formation at the periphery. These results collectively demonstrated that gelatin-coating of porous PCL scaffolds is a promising approach for delivering rhBMP-2 to stimulate improved Bone regeneration.

  • In Situ Controlled Release of rhBMP-2 in Gelatin-Coated 3D Porous Poly(ε-caprolactone) Scaffolds for Homogeneous Bone Tissue Formation
    Biomacromolecules, 2013
    Co-Authors: Qingchun Zhang, Yan Zhang, Zhaoyang Ye, Meidong Lang

    Abstract:

    In Tissue engineering, incorporation of Bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate Bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable Bone Formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(e-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better Bone format…

Qingchun Zhang – One of the best experts on this subject based on the ideXlab platform.

  • in situ controlled release of rhbmp 2 in gelatin coated 3d porous poly e caprolactone scaffolds for homogeneous Bone Tissue Formation
    Biomacromolecules, 2014
    Co-Authors: Qingchun Zhang, Yan Zhang, Zhaoyang Ye, Meidong Lang

    Abstract:

    In Tissue engineering, incorporation of Bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate Bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable Bone Formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(e-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better Bone format…

  • In situ controlled release of rhBMP-2 in gelatin-coated 3D porous poly(ε-caprolactone) scaffolds for homogeneous Bone Tissue Formation
    Biomacromolecules, 2014
    Co-Authors: Qingchun Zhang, Ke Tan, Yan Zhang, Zhaoyang Ye, Wen-song Tan, Meidong Lang

    Abstract:

    In Tissue engineering, incorporation of Bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate Bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable Bone Formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(ε-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better Bone Formation was observed in gelatin/rhBMP-2-coated scaffolds. Specifically, the spatial distribution of newly formed Bone was more uniform in gelatin-coated scaffolds than in uncoated scaffolds, which displayed preferential Bone Formation at the periphery. These results collectively demonstrated that gelatin-coating of porous PCL scaffolds is a promising approach for delivering rhBMP-2 to stimulate improved Bone regeneration.

  • In Situ Controlled Release of rhBMP-2 in Gelatin-Coated 3D Porous Poly(ε-caprolactone) Scaffolds for Homogeneous Bone Tissue Formation
    Biomacromolecules, 2013
    Co-Authors: Qingchun Zhang, Yan Zhang, Zhaoyang Ye, Meidong Lang

    Abstract:

    In Tissue engineering, incorporation of Bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate Bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable Bone Formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(e-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better Bone format…

Gordana Vunjak-novakovic – One of the best experts on this subject based on the ideXlab platform.

  • THE INFLUENCE OF SILK FIBROIN 3D SCAFFOLD COMPOSITION FOR IN VITRO Bone Tissue ENGINEERING
    , 2020
    Co-Authors: Cristina Correia, Gordana Vunjak-novakovic, Sarindr Bhumiratana, Jeffery M Gimble, David L. Kaplan, Rui A. Sousa, Yan Leping, Ana Oliveira, Rui L Reis

    Abstract:

    Scaffold composition, configuration and resulting properties critically affects Tissue development. In this study, we evaluated the influence of silk fibroin concentration (6 or 17%) and correspondent processing method (aqueous or HFIP-derived) and three- dimensional scaffold structure (lamellar or porous, with distinct pore size) on Bone Tissue Formation by osteogenic differentiation of human adipose Tissue derived stem cells (hASC). We observed that very similar Bone Tissue was formed in all silk fibroin scaffold groups, evaluated by alkaline phosphatase activity, calcium production, collagen type I deposition and scaffold Bone volume fraction.

  • Development of silk-based scaffolds for Tissue engineering of Bone from human adipose derived stem cells
    Acta Biomaterialia, 2012
    Co-Authors: Cristina Correia, Sarindr Bhumiratana, Jeffery M Gimble, Danielle Rockwood, David L. Kaplan, Rui A. Sousa, Rui L Reis, Ana Oliveira, Gordana Vunjak-novakovic

    Abstract:

    Silk fibroin is a potent alternative to other biodegradable biopolymers for Bone Tissue engineering (TE), because of its tunable architecture and mechanical properties, and its demonstrated ability to support Bone Formation both in vitro and in vivo. In this study, we investigated a range of silk scaffolds for Bone TE using human adipose-derived stem cells (hASCs), an attractive cell source for engineering autologous Bone grafts. Our goal was to understand the effects of scaffold architecture and biomechanics and use this inFormation to optimize silk scaffolds for Bone TE applications. Silk scaffolds were fabricated using differ- ent solvents (aqueous vs. hexafluoro-2-propanol (HFIP)), pore sizes (250-500 lm vs. 500-1000 lm) and structures (lamellar vs. spherical pores). Four types of silk scaffolds combining the properties of interest were systematically compared with respect to Bone Tissue outcomes, with decellularized trabecular Bone (DCB) included as a ”gold standard”. The scaffolds were seeded with hASCs and cultured for 7 weeks in osteogenic medium. Bone Formation was evaluated by cell proliferation and differentiation, matrix pro- duction, calcification and mechanical properties. We observed that 400-600 lm porous HFIP-derived silk fibroin scaffold demonstrated the best Bone Tissue Formation outcomes, as evidenced by increased Bone protein production (osteopontin, collagen type I, Bone sialoprotein), enhanced calcium deposition and total Bone volume. On a direct comparison basis, alkaline phosphatase activity (AP) at week 2 and new calcium deposition at week 7 were comparable to the cells cultured in DCB. Yet, among the aqueous- based structures, the lamellar architecture induced increased AP activity and demonstrated higher equi- librium modulus than the spherical-pore scaffolds. Based on the collected data, we propose a conceptual model describing the effects of silk scaffold design on Bone Tissue Formation.

  • Development of silk-based scaffolds for Tissue engineering of Bone from human adipose-derived stem cells
    Acta Biomaterialia, 2012
    Co-Authors: Cristina Correia, Sarindr Bhumiratana, Le Ping Yan, Ana L. Oliveira, Jeffery M Gimble, Danielle Rockwood, David L. Kaplan, Rui A. Sousa, Rui L Reis, Gordana Vunjak-novakovic

    Abstract:

    Silk fibroin is a potent alternative to other biodegradable biopolymers for Bone Tissue engineering (TE), because of its tunable architecture and mechanical properties, and its demonstrated ability to support Bone Formation both in vitro and in vivo. In this study, we investigated a range of silk scaffolds for Bone TE using human adipose-derived stem cells (hASCs), an attractive cell source for engineering autologous Bone grafts. Our goal was to understand the effects of scaffold architecture and biomechanics and use this inFormation to optimize silk scaffolds for Bone TE applications. Silk scaffolds were fabricated using different solvents (aqueous vs. hexafluoro-2-propanol (HFIP)), pore sizes (250-500 μm vs. 500-1000 μm) and structures (lamellar vs. spherical pores). Four types of silk scaffolds combining the properties of interest were systematically compared with respect to Bone Tissue outcomes, with decellularized trabecular Bone (DCB) included as a “gold standard”. The scaffolds were seeded with hASCs and cultured for 7 weeks in osteogenic medium. Bone Formation was evaluated by cell proliferation and differentiation, matrix production, calcification and mechanical properties. We observed that 400-600 μm porous HFIP-derived silk fibroin scaffold demonstrated the best Bone Tissue Formation outcomes, as evidenced by increased Bone protein production (osteopontin, collagen type I, Bone sialoprotein), enhanced calcium deposition and total Bone volume. On a direct comparison basis, alkaline phosphatase activity (AP) at week 2 and new calcium deposition at week 7 were comparable to the cells cultured in DCB. Yet, among the aqueous-based structures, the lamellar architecture induced increased AP activity and demonstrated higher equilibrium modulus than the spherical-pore scaffolds. Based on the collected data, we propose a conceptual model describing the effects of silk scaffold design on Bone Tissue Formation. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.