The Experts below are selected from a list of 258105 Experts worldwide ranked by ideXlab platform
Se-kwon Kim - One of the best experts on this subject based on the ideXlab platform.
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Nano-hydroxyapatite composite biomaterials for Bone Tissue engineering - A review
Journal of Biomedical Nanotechnology, 2014Co-Authors: Jayachandran Venkatesan, Se-kwon KimAbstract:In recent years, significant development has been achieved in the construction of artificial Bone with ceramics, polymers and metals. Nano-hydroxyapatite (nHA) is widely used bioceramic material for Bone graft substitute owing to its biocompatibility and osteoconductive properties. nHA with chitin, chitosan, collagen, gelatin, fibrin, polylactic acid, polycaprolactone, poly(lactic-co-glycolic) acid, polyamide, polyvinyl alcohol, polyurethane and polyhydroxybutyrate based composite scaffolds have been explored in the present review for Bone graft substitute. This article further reviews the preparative methods, chemical interaction, biocompatibiity, biodegradation, alkaline phosphatase activity, mineralization effect, mechanical properties and delivery of nHA-based nanocomposites for Bone Tissue regeneration. The nHA based composite biomaterials proved to be promising biomaterials for Bone Tissue engineering.
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Alginate composites for Bone Tissue engineering: A review.
International journal of biological macromolecules, 2014Co-Authors: Jayachandran Venkatesan, Kyong-hwa Kang, Panchanathan Manivasagan, Ira Bhatnagar, Se-kwon KimAbstract:Bone is a complex and hierarchical Tissue consisting of nano hydroxyapatite and collagen as major portion. Several attempts have been made to prepare the artificial Bone so as to replace the autograft and allograft treatment. Tissue engineering is a promising approach to solve the several issues and is also useful in the construction of artificial Bone with materials including polymer, ceramics, metals, cells and growth factors. Composites consisting of polymer-ceramics, best mimic the natural functions of Bone. Alginate, an anionic polymer owing enormous biomedical applications, is gaining importance particularly in Bone Tissue engineering due to its biocompatibility and gel forming properties. Several composites such as alginate-polymer (PLGA, PEG and chitosan), alginate-protein (collagen and gelatin), alginate-ceramic, alginate-bioglass, alginate-biosilica, alginate-Bone morphogenetic protein-2 and RGD peptides composite have been investigated till date. These alginate composites show enhanced biochemical significance in terms of porosity, mechanical strength, cell adhesion, biocompatibility, cell proliferation, alkaline phosphatase increase, excellent mineralization and osteogenic differentiation. Hence, alginate based composite biomaterials will be promising for Bone Tissue regeneration. This review will provide a broad overview of alginate preparation and its applications towards Bone Tissue engineering.
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Chitosan composites for Bone Tissue engineering - An overview
Marine Drugs, 2010Co-Authors: Jayachandran Venkatesan, Se-kwon KimAbstract:Bone contains considerable amounts of minerals and proteins. Hydroxyapatite [Ca₁₀(PO₄)₆(OH)₂] is one of the most stable forms of calcium phosphate and it occurs in Bones as major component (60 to 65%), along with other materials including collagen, chondroitin sulfate, keratin sulfate and lipids. In recent years, significant progress has been made in organ transplantation, surgical reconstruction and the use of artificial prostheses to treat the loss or failure of an organ or Bone Tissue. Chitosan has played a major role in Bone Tissue engineering over the last two decades, being a natural polymer obtained from chitin, which forms a major component of crustacean exoskeleton. In recent years, considerable attention has been given to chitosan composite materials and their applications in the field of Bone Tissue engineering due to its minimal foreign body reactions, an intrinsic antibacterial nature, biocompatibility, biodegradability, and the ability to be molded into various geometries and forms such as porous structures, suitable for cell ingrowth and osteoconduction. The composite of chitosan including hydroxyapatite is very popular because of the biodegradability and biocompatibility in nature. Recently, grafted chitosan natural polymer with carbon nanotubes has been incorporated to increase the mechanical strength of these composites. Chitosan composites are thus emerging as potential materials for artificial Bone and Bone regeneration in Tissue engineering. Herein, the preparation, mechanical properties, chemical interactions and in vitro activity of chitosan composites for Bone Tissue engineering will be discussed.
N Selvamurugan - One of the best experts on this subject based on the ideXlab platform.
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chitosan based biocomposite scaffolds for Bone Tissue engineering
International Journal of Biological Macromolecules, 2016Co-Authors: S Saravanan, R S Leena, N SelvamuruganAbstract:The clinical demand for scaffolds and the diversity of available polymers provide freedom in the fabrication of scaffolds to achieve successful progress in Bone Tissue engineering (BTE). Chitosan (CS) has drawn much of the attention in recent years for its use as graft material either as alone or in a combination with other materials in BTE. The scaffolds should possess a number of properties like porosity, biocompatibility, water retention, protein adsorption, mechanical strength, biomineralization and biodegradability suited for BTE applications. In this review, CS and its properties, and the role of CS along with other polymeric and ceramic materials as scaffolds for Bone Tissue repair applications are highlighted.
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a novel injectable temperature sensitive zinc doped chitosan β glycerophosphate hydrogel for Bone Tissue engineering
International Journal of Biological Macromolecules, 2013Co-Authors: Ramesh Niranjan, A Moorthi, Chandru Koushik, Sekaran Saravanan, M Vairamani, N SelvamuruganAbstract:Hydrogels are hydrophilic polymers that have a wide range of biomedical applications including Bone Tissue engineering. In this study we report preparation and characterization of a thermosensitive hydrogel (Zn-CS/β-GP) containing zinc (Zn), chitosan (CS) and beta-glycerophosphate (β-GP) for Bone Tissue engineering. The prepared hydrogel exhibited a liquid state at room temperature and turned into a gel at body temperature. The hydrogel was characterized by SEM, EDX, XRD, FT-IR and swelling studies. The hydrogel enhanced antibacterial activity and promoted osteoblast differentiation. Thus, we suggest that the Zn-CS/β-GP hydrogel could have potential impact as an injectable in situ forming scaffold for Bone Tissue engineering applications.
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enhanced osteoblast adhesion on polymeric nano scaffolds for Bone Tissue engineering
Journal of Biomedical Nanotechnology, 2011Co-Authors: N Saranya, A Moorthi, Sekaran Saravanan, B Ramyakrishna, N SelvamuruganAbstract:Bone Tissue engineering is an interdisciplinary field which is emerged for the development of viable substitutes that restore and maintain the function of human Bone Tissues. The success of Bone Tissue engineering depends on designing of the scaffolds. The polymer-based composite scaffolds containing micro- and nano-structures could provide a platform influencing osteoblastic cell adhesion, spreading, proliferation, and differentiation. Osteoblasts may adhere strongly to the nano-structures than micro-structures in the scaffolds due to the large surface area, better osteo-integrative property and mechanical reliability etc. In this review we are focusing the factors such as pore size, surface topography and roughness, protein adsorption and wettability of nano-structures and their interaction with cell surface integrins molecules. A better understanding of the interactions of nano-structures with osteoblastic cells will have potential applications in the regeneration of Bone.
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biocomposites containing natural polymers and hydroxyapatite for Bone Tissue engineering
International Journal of Biological Macromolecules, 2010Co-Authors: Maddela Swetha, Kolli Sahithi, A Moorthi, N Srinivasan, Kumarasamy Ramasamy, N SelvamuruganAbstract:Bone Tissue engineering is an alternative strategy to generate Bone utilizing a combination of biomaterials and cells. Biomaterials that mimic the structure and composition of Bone Tissues at nanoscale are important for the development of Bone Tissue engineering applications. Natural or biopolymer-based composites containing chitin, chitosan, or collagen have advantages such as biocompatibility, biodegradability that are essential for Bone Tissue engineering. The inclusion of nanoparticles of hydroxyapatite (one of the most widely used bioceramic materials) into the biopolymer matrix improves the mechanical properties and incorporates the nanotopographic features that mimic the nanostructure of Bone. This review summarizes the recent work on the development of biocomposites containing natural polymers with hydroxyapatite particles suitable for use in Bone defects/Bone regeneration.
Jayachandran Venkatesan - One of the best experts on this subject based on the ideXlab platform.
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Nano-hydroxyapatite composite biomaterials for Bone Tissue engineering - A review
Journal of Biomedical Nanotechnology, 2014Co-Authors: Jayachandran Venkatesan, Se-kwon KimAbstract:In recent years, significant development has been achieved in the construction of artificial Bone with ceramics, polymers and metals. Nano-hydroxyapatite (nHA) is widely used bioceramic material for Bone graft substitute owing to its biocompatibility and osteoconductive properties. nHA with chitin, chitosan, collagen, gelatin, fibrin, polylactic acid, polycaprolactone, poly(lactic-co-glycolic) acid, polyamide, polyvinyl alcohol, polyurethane and polyhydroxybutyrate based composite scaffolds have been explored in the present review for Bone graft substitute. This article further reviews the preparative methods, chemical interaction, biocompatibiity, biodegradation, alkaline phosphatase activity, mineralization effect, mechanical properties and delivery of nHA-based nanocomposites for Bone Tissue regeneration. The nHA based composite biomaterials proved to be promising biomaterials for Bone Tissue engineering.
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Alginate composites for Bone Tissue engineering: A review.
International journal of biological macromolecules, 2014Co-Authors: Jayachandran Venkatesan, Kyong-hwa Kang, Panchanathan Manivasagan, Ira Bhatnagar, Se-kwon KimAbstract:Bone is a complex and hierarchical Tissue consisting of nano hydroxyapatite and collagen as major portion. Several attempts have been made to prepare the artificial Bone so as to replace the autograft and allograft treatment. Tissue engineering is a promising approach to solve the several issues and is also useful in the construction of artificial Bone with materials including polymer, ceramics, metals, cells and growth factors. Composites consisting of polymer-ceramics, best mimic the natural functions of Bone. Alginate, an anionic polymer owing enormous biomedical applications, is gaining importance particularly in Bone Tissue engineering due to its biocompatibility and gel forming properties. Several composites such as alginate-polymer (PLGA, PEG and chitosan), alginate-protein (collagen and gelatin), alginate-ceramic, alginate-bioglass, alginate-biosilica, alginate-Bone morphogenetic protein-2 and RGD peptides composite have been investigated till date. These alginate composites show enhanced biochemical significance in terms of porosity, mechanical strength, cell adhesion, biocompatibility, cell proliferation, alkaline phosphatase increase, excellent mineralization and osteogenic differentiation. Hence, alginate based composite biomaterials will be promising for Bone Tissue regeneration. This review will provide a broad overview of alginate preparation and its applications towards Bone Tissue engineering.
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Chitosan composites for Bone Tissue engineering - An overview
Marine Drugs, 2010Co-Authors: Jayachandran Venkatesan, Se-kwon KimAbstract:Bone contains considerable amounts of minerals and proteins. Hydroxyapatite [Ca₁₀(PO₄)₆(OH)₂] is one of the most stable forms of calcium phosphate and it occurs in Bones as major component (60 to 65%), along with other materials including collagen, chondroitin sulfate, keratin sulfate and lipids. In recent years, significant progress has been made in organ transplantation, surgical reconstruction and the use of artificial prostheses to treat the loss or failure of an organ or Bone Tissue. Chitosan has played a major role in Bone Tissue engineering over the last two decades, being a natural polymer obtained from chitin, which forms a major component of crustacean exoskeleton. In recent years, considerable attention has been given to chitosan composite materials and their applications in the field of Bone Tissue engineering due to its minimal foreign body reactions, an intrinsic antibacterial nature, biocompatibility, biodegradability, and the ability to be molded into various geometries and forms such as porous structures, suitable for cell ingrowth and osteoconduction. The composite of chitosan including hydroxyapatite is very popular because of the biodegradability and biocompatibility in nature. Recently, grafted chitosan natural polymer with carbon nanotubes has been incorporated to increase the mechanical strength of these composites. Chitosan composites are thus emerging as potential materials for artificial Bone and Bone regeneration in Tissue engineering. Herein, the preparation, mechanical properties, chemical interactions and in vitro activity of chitosan composites for Bone Tissue engineering will be discussed.
Antonios G. Mikos - One of the best experts on this subject based on the ideXlab platform.
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Materials design for Bone-Tissue engineering
Nature Reviews Materials, 2020Co-Authors: Gerry L. Koons, Mani Diba, Antonios G. MikosAbstract:Design of Bone-Tissue-engineering materials involves consideration of multiple, often conflicting, requirements. This Review discusses these considerations and highlights scalable technologies that can fabricate natural and synthetic biomaterials (polymers, bioceramics, metals and composites) into forms suitable for Bone-Tissue-engineering applications in human therapies and disease models. Successful materials design for Bone-Tissue engineering requires an understanding of the composition and structure of native Bone Tissue, as well as appropriate selection of biomimetic natural or tunable synthetic materials (biomaterials), such as polymers, bioceramics, metals and composites. Scalable fabrication technologies that enable control over construct architecture at multiple length scales, including three-dimensional printing and electric-field-assisted techniques, can then be employed to process these biomaterials into suitable forms for Bone-Tissue engineering. In this Review, we provide an overview of materials-design considerations for Bone-Tissue-engineering applications in both disease modelling and treatment of injuries and disease in humans. We outline the materials-design pathway from implementation strategy through selection of materials and fabrication methods to evaluation. Finally, we discuss unmet needs and current challenges in the development of ideal materials for Bone-Tissue regeneration and highlight emerging strategies in the field.
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review mineralization of synthetic polymer scaffolds for Bone Tissue engineering
Tissue Engineering, 2007Co-Authors: James D Kretlow, Antonios G. MikosAbstract:It has repeatedly been shown that demineralization improves the ability of Bone auto- and allografts to regenerate natural Bone Tissue. Conversely, much work in the field of Bone Tissue engineering...
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review mineralization of synthetic polymer scaffolds for Bone Tissue engineering
Tissue Engineering, 2007Co-Authors: James D Kretlow, Antonios G. MikosAbstract:It has repeatedly been shown that demineralization improves the ability of Bone auto- and allografts to regenerate natural Bone Tissue. Conversely, much work in the field of Bone Tissue engineering has used composite materials consisting of a mineralized phase or materials designed to mineralize rapidly in situ. In this review, we seek to examine these disparate roles of mineralization and the underlying factors that cause this discordance and to examine methods and principles of the mineralization of synthetic polymer scaffolds. Biomimetic approaches to mineralization and phosphorus-containing materials are highlighted, and a brief section focusing on drug-delivery strategies using mineralized scaffolds is included.
S. Ramakrishna - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous electrospin-electrosprayed biocomposite nanofibrous scaffolds for Bone Tissue regeneration
Acta Biomaterialia, 2010Co-Authors: Lijo Francis, Jayarama Reddy Venugopal, Molamma P. Prabhakaran, Velmurugan Thavasi, Enrico Marsano, S. RamakrishnaAbstract:Abstract Currently, the application of nanotechnology in Bone Tissue regeneration is a challenge for the fabrication of novel bioartificial Bone grafts. These nanostructures are capable of mimicking natural extracellular matrix with effective mineralization for successful regeneration of damaged Tissues. The simultaneous electrospraying of nanohydroxyapatite (HA) on electrospun polymeric nanofibrous scaffolds might be more promising for Bone Tissue regeneration. In the current study, nanofibrous scaffolds of gelatin (Gel), Gel/HA (4:1 blend), Gel/HA (2:1 blend) and Gel/HA (electrospin–electrospray) were fabricated for this purpose. The morphology, chemical and mechanical stability of nanofibres were evaluated by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy and with a universal tensile machine, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was determined by culturing human foetal osteoblasts and investigating the proliferation, alkaline phosphatase (ALP) activity and mineralization of cells. The results of cell proliferation, ALP activity and FESEM studies revealed that the combination of electrospinning of gelatin and electrospraying of HA yielded biocomposite nanofibrous scaffolds with enhanced performances in terms of better cell proliferation, increased ALP activity and enhanced mineralization, making them potential substrates for Bone Tissue regeneration.
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electrospun nanostructured scaffolds for Bone Tissue engineering
Acta Biomaterialia, 2009Co-Authors: Molamma P. Prabhakaran, Jayarama Reddy Venugopal, S. RamakrishnaAbstract:Abstract The current challenge in Bone Tissue engineering is to fabricate a bioartificial Bone graft mimicking the extracellular matrix (ECM) with effective Bone mineralization, resulting in the regeneration of fractured or diseased Bones. Biocomposite polymeric nanofibers containing nanohydroxyapatite (HA) fabricated by electrospinning could be promising scaffolds for Bone Tissue engineering. Nanofibrous scaffolds of poly- l -lactide (PLLA, 860 ± 110 nm), PLLA/HA (845 ± 140 nm) and PLLA/collagen/HA (310 ± 125 nm) were fabricated, and the morphology, chemical and mechanical characterization of the nanofibers were evaluated using scanning electron microscopy, Fourier transform infrared spectroscopy and tensile testing, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was also assessed by growing human fetal osteoblasts (hFOB), and investigating the proliferation, alkaline phosphatase activity (ALP) and mineralization of cells on different nanofibrous scaffolds. Osteoblasts were found to adhere and grow actively on PLLA/collagen/HA nanofibers with enhanced mineral deposition of 57% higher than the PLLA/HA nanofibers. The synergistic effect of the presence of an ECM protein, collagen and HA in PLLA/collagen/HA nanofibers provided cell recognition sites together with apatite for cell proliferation and osteoconduction necessary for mineralization and Bone formation. The results of our study showed that the biocomposite PLLA/collagen/HA nanofibrous scaffold could be a potential substrate for the proliferation and mineralization of osteoblasts, enhancing Bone regeneration.
