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Hernâni Geros - One of the best experts on this subject based on the ideXlab platform.

  • changes in the volatile composition of wine from grapes treated with Bordeaux Mixture a laboratory scale study
    Australian Journal of Grape and Wine Research, 2015
    Co-Authors: Viviana Martins, Antonio Teixeira, Hernâni Geros
    Abstract:

    This work was supported by European Union Funds (FEDER/COMPETE-Operational Competitiveness Programme) and by Portuguese national funds (FCT - Portuguese Foundation for Science and Technology) under the project FCOMP-01-0124-FEDER-022692, the research project FCOMP-01-124-FEDER-008760 (Ref. FCT PTDC/AGR-ALI/100636/2008), the project PEst-OE/AGR/UI4033/2014 and the PhD grant no. SFRH/BD/64587/2009 to Dr Viviana Martins. This work also benefited from the networking activities within the European project INNOVINE (ref. 311775) and the European COST ACTION (FA1106 'QualityFruit').

  • copper based fungicide Bordeaux Mixture regulates the expression of vitis vinifera copper transporters
    Australian Journal of Grape and Wine Research, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Mohsen Hanana, Hernâni Geros
    Abstract:

    Background and Aims: The Bordeaux Mixture has been widely used in viticulture, but the response of grape cells (Vitis vinifera L.) to copper is still largely unknown. In the present study, we investigated if copper application in the vineyard could interfere with copper homeostasis through the regulation of the expression of copper transporters (VvCTrs). Methods and Results: The VvCTrs characterised in silico are phylogenetically related to COPTs from Arabidopsis and rice. In a field trial, we studied the expression of each VvCTr in grape berries and leaves throughout the season by quantitative real-time polymerase chain reaction. Transcripts of VvCTr1 and VvCTr8 were the most abundant in leaves and berries, respectively, while VvCTrs 4, 5 and 7 were the least expressed, but the application of copper resulted in a significant change in the transcript levels. In plants treated with Bordeaux Mixture, the copper concentration increased twofold in the pulp and ninefold on the skin of surface-washed mature berries. Conclusions: The expression pattern of VvCTr1-8 depends on the tissue and developmental stage. Four applications of copper following a methodology used in organic farming are sufficient to induce a significant increase in copper content in berry tissues which is either a cause or a consequence of the observed transcriptional reprogramming of the expression of the VvCTrs. Significance of the Study: The application of Bordeaux Mixture should have major consequences on grapevine copper homeostasis, regulating copper transport at the transcriptional level. A new research avenue emerged towards the characterisation of VvCTr-mediated transport at the molecular level in response to copper.

  • metabolic changes of vitis vinifera berries and leaves exposed to Bordeaux Mixture
    Plant Physiology and Biochemistry, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Hernâni Geros
    Abstract:

    Since the development of Bordeaux Mixture in the late 1800's, copper-based fungicides have been widely used against grapevine (Vitis vinifera L.) diseases, mainly in organic but also in conventional viticulture; however their intensive use has raised phytotoxicity concerns. In this study, the composition of grape berries and leaves upon Bordeaux Mixture treatment was investigated during the fructification season by a metabolomic approach. Four applications of Bordeaux Mixture till 3 weeks before harvest were performed following the regular management practices of organic viticulture. Results showed that the copper-based treatment affected the content in sugars, organic acids, lipids and flavan-3-ols of grapes and leaves at specific developmental stages. Nonetheless, the levels of sucrose, glucose and fructose, and of tartaric and malic acids were not significantly affected in mature grapes. In contrast, a sharp decrease in free natural amino acids was observed, together with a reduction in protein content and in mineral nitrogen forms. The treatment with Bordeaux Mixture increased by 7-fold the copper levels in tissue extracts from surface-washed mature berries.

Viviana Martins - One of the best experts on this subject based on the ideXlab platform.

  • changes in the volatile composition of wine from grapes treated with Bordeaux Mixture a laboratory scale study
    Australian Journal of Grape and Wine Research, 2015
    Co-Authors: Viviana Martins, Antonio Teixeira, Hernâni Geros
    Abstract:

    This work was supported by European Union Funds (FEDER/COMPETE-Operational Competitiveness Programme) and by Portuguese national funds (FCT - Portuguese Foundation for Science and Technology) under the project FCOMP-01-0124-FEDER-022692, the research project FCOMP-01-124-FEDER-008760 (Ref. FCT PTDC/AGR-ALI/100636/2008), the project PEst-OE/AGR/UI4033/2014 and the PhD grant no. SFRH/BD/64587/2009 to Dr Viviana Martins. This work also benefited from the networking activities within the European project INNOVINE (ref. 311775) and the European COST ACTION (FA1106 'QualityFruit').

  • copper based fungicide Bordeaux Mixture regulates the expression of vitis vinifera copper transporters
    Australian Journal of Grape and Wine Research, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Mohsen Hanana, Hernâni Geros
    Abstract:

    Background and Aims: The Bordeaux Mixture has been widely used in viticulture, but the response of grape cells (Vitis vinifera L.) to copper is still largely unknown. In the present study, we investigated if copper application in the vineyard could interfere with copper homeostasis through the regulation of the expression of copper transporters (VvCTrs). Methods and Results: The VvCTrs characterised in silico are phylogenetically related to COPTs from Arabidopsis and rice. In a field trial, we studied the expression of each VvCTr in grape berries and leaves throughout the season by quantitative real-time polymerase chain reaction. Transcripts of VvCTr1 and VvCTr8 were the most abundant in leaves and berries, respectively, while VvCTrs 4, 5 and 7 were the least expressed, but the application of copper resulted in a significant change in the transcript levels. In plants treated with Bordeaux Mixture, the copper concentration increased twofold in the pulp and ninefold on the skin of surface-washed mature berries. Conclusions: The expression pattern of VvCTr1-8 depends on the tissue and developmental stage. Four applications of copper following a methodology used in organic farming are sufficient to induce a significant increase in copper content in berry tissues which is either a cause or a consequence of the observed transcriptional reprogramming of the expression of the VvCTrs. Significance of the Study: The application of Bordeaux Mixture should have major consequences on grapevine copper homeostasis, regulating copper transport at the transcriptional level. A new research avenue emerged towards the characterisation of VvCTr-mediated transport at the molecular level in response to copper.

  • metabolic changes of vitis vinifera berries and leaves exposed to Bordeaux Mixture
    Plant Physiology and Biochemistry, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Hernâni Geros
    Abstract:

    Since the development of Bordeaux Mixture in the late 1800's, copper-based fungicides have been widely used against grapevine (Vitis vinifera L.) diseases, mainly in organic but also in conventional viticulture; however their intensive use has raised phytotoxicity concerns. In this study, the composition of grape berries and leaves upon Bordeaux Mixture treatment was investigated during the fructification season by a metabolomic approach. Four applications of Bordeaux Mixture till 3 weeks before harvest were performed following the regular management practices of organic viticulture. Results showed that the copper-based treatment affected the content in sugars, organic acids, lipids and flavan-3-ols of grapes and leaves at specific developmental stages. Nonetheless, the levels of sucrose, glucose and fructose, and of tartaric and malic acids were not significantly affected in mature grapes. In contrast, a sharp decrease in free natural amino acids was observed, together with a reduction in protein content and in mineral nitrogen forms. The treatment with Bordeaux Mixture increased by 7-fold the copper levels in tissue extracts from surface-washed mature berries.

Antonio Teixeira - One of the best experts on this subject based on the ideXlab platform.

  • changes in the volatile composition of wine from grapes treated with Bordeaux Mixture a laboratory scale study
    Australian Journal of Grape and Wine Research, 2015
    Co-Authors: Viviana Martins, Antonio Teixeira, Hernâni Geros
    Abstract:

    This work was supported by European Union Funds (FEDER/COMPETE-Operational Competitiveness Programme) and by Portuguese national funds (FCT - Portuguese Foundation for Science and Technology) under the project FCOMP-01-0124-FEDER-022692, the research project FCOMP-01-124-FEDER-008760 (Ref. FCT PTDC/AGR-ALI/100636/2008), the project PEst-OE/AGR/UI4033/2014 and the PhD grant no. SFRH/BD/64587/2009 to Dr Viviana Martins. This work also benefited from the networking activities within the European project INNOVINE (ref. 311775) and the European COST ACTION (FA1106 'QualityFruit').

  • copper based fungicide Bordeaux Mixture regulates the expression of vitis vinifera copper transporters
    Australian Journal of Grape and Wine Research, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Mohsen Hanana, Hernâni Geros
    Abstract:

    Background and Aims: The Bordeaux Mixture has been widely used in viticulture, but the response of grape cells (Vitis vinifera L.) to copper is still largely unknown. In the present study, we investigated if copper application in the vineyard could interfere with copper homeostasis through the regulation of the expression of copper transporters (VvCTrs). Methods and Results: The VvCTrs characterised in silico are phylogenetically related to COPTs from Arabidopsis and rice. In a field trial, we studied the expression of each VvCTr in grape berries and leaves throughout the season by quantitative real-time polymerase chain reaction. Transcripts of VvCTr1 and VvCTr8 were the most abundant in leaves and berries, respectively, while VvCTrs 4, 5 and 7 were the least expressed, but the application of copper resulted in a significant change in the transcript levels. In plants treated with Bordeaux Mixture, the copper concentration increased twofold in the pulp and ninefold on the skin of surface-washed mature berries. Conclusions: The expression pattern of VvCTr1-8 depends on the tissue and developmental stage. Four applications of copper following a methodology used in organic farming are sufficient to induce a significant increase in copper content in berry tissues which is either a cause or a consequence of the observed transcriptional reprogramming of the expression of the VvCTrs. Significance of the Study: The application of Bordeaux Mixture should have major consequences on grapevine copper homeostasis, regulating copper transport at the transcriptional level. A new research avenue emerged towards the characterisation of VvCTr-mediated transport at the molecular level in response to copper.

  • metabolic changes of vitis vinifera berries and leaves exposed to Bordeaux Mixture
    Plant Physiology and Biochemistry, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Hernâni Geros
    Abstract:

    Since the development of Bordeaux Mixture in the late 1800's, copper-based fungicides have been widely used against grapevine (Vitis vinifera L.) diseases, mainly in organic but also in conventional viticulture; however their intensive use has raised phytotoxicity concerns. In this study, the composition of grape berries and leaves upon Bordeaux Mixture treatment was investigated during the fructification season by a metabolomic approach. Four applications of Bordeaux Mixture till 3 weeks before harvest were performed following the regular management practices of organic viticulture. Results showed that the copper-based treatment affected the content in sugars, organic acids, lipids and flavan-3-ols of grapes and leaves at specific developmental stages. Nonetheless, the levels of sucrose, glucose and fructose, and of tartaric and malic acids were not significantly affected in mature grapes. In contrast, a sharp decrease in free natural amino acids was observed, together with a reduction in protein content and in mineral nitrogen forms. The treatment with Bordeaux Mixture increased by 7-fold the copper levels in tissue extracts from surface-washed mature berries.

Elias Bassil - One of the best experts on this subject based on the ideXlab platform.

  • copper based fungicide Bordeaux Mixture regulates the expression of vitis vinifera copper transporters
    Australian Journal of Grape and Wine Research, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Mohsen Hanana, Hernâni Geros
    Abstract:

    Background and Aims: The Bordeaux Mixture has been widely used in viticulture, but the response of grape cells (Vitis vinifera L.) to copper is still largely unknown. In the present study, we investigated if copper application in the vineyard could interfere with copper homeostasis through the regulation of the expression of copper transporters (VvCTrs). Methods and Results: The VvCTrs characterised in silico are phylogenetically related to COPTs from Arabidopsis and rice. In a field trial, we studied the expression of each VvCTr in grape berries and leaves throughout the season by quantitative real-time polymerase chain reaction. Transcripts of VvCTr1 and VvCTr8 were the most abundant in leaves and berries, respectively, while VvCTrs 4, 5 and 7 were the least expressed, but the application of copper resulted in a significant change in the transcript levels. In plants treated with Bordeaux Mixture, the copper concentration increased twofold in the pulp and ninefold on the skin of surface-washed mature berries. Conclusions: The expression pattern of VvCTr1-8 depends on the tissue and developmental stage. Four applications of copper following a methodology used in organic farming are sufficient to induce a significant increase in copper content in berry tissues which is either a cause or a consequence of the observed transcriptional reprogramming of the expression of the VvCTrs. Significance of the Study: The application of Bordeaux Mixture should have major consequences on grapevine copper homeostasis, regulating copper transport at the transcriptional level. A new research avenue emerged towards the characterisation of VvCTr-mediated transport at the molecular level in response to copper.

  • metabolic changes of vitis vinifera berries and leaves exposed to Bordeaux Mixture
    Plant Physiology and Biochemistry, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Hernâni Geros
    Abstract:

    Since the development of Bordeaux Mixture in the late 1800's, copper-based fungicides have been widely used against grapevine (Vitis vinifera L.) diseases, mainly in organic but also in conventional viticulture; however their intensive use has raised phytotoxicity concerns. In this study, the composition of grape berries and leaves upon Bordeaux Mixture treatment was investigated during the fructification season by a metabolomic approach. Four applications of Bordeaux Mixture till 3 weeks before harvest were performed following the regular management practices of organic viticulture. Results showed that the copper-based treatment affected the content in sugars, organic acids, lipids and flavan-3-ols of grapes and leaves at specific developmental stages. Nonetheless, the levels of sucrose, glucose and fructose, and of tartaric and malic acids were not significantly affected in mature grapes. In contrast, a sharp decrease in free natural amino acids was observed, together with a reduction in protein content and in mineral nitrogen forms. The treatment with Bordeaux Mixture increased by 7-fold the copper levels in tissue extracts from surface-washed mature berries.

Eduardo Blumwald - One of the best experts on this subject based on the ideXlab platform.

  • copper based fungicide Bordeaux Mixture regulates the expression of vitis vinifera copper transporters
    Australian Journal of Grape and Wine Research, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Mohsen Hanana, Hernâni Geros
    Abstract:

    Background and Aims: The Bordeaux Mixture has been widely used in viticulture, but the response of grape cells (Vitis vinifera L.) to copper is still largely unknown. In the present study, we investigated if copper application in the vineyard could interfere with copper homeostasis through the regulation of the expression of copper transporters (VvCTrs). Methods and Results: The VvCTrs characterised in silico are phylogenetically related to COPTs from Arabidopsis and rice. In a field trial, we studied the expression of each VvCTr in grape berries and leaves throughout the season by quantitative real-time polymerase chain reaction. Transcripts of VvCTr1 and VvCTr8 were the most abundant in leaves and berries, respectively, while VvCTrs 4, 5 and 7 were the least expressed, but the application of copper resulted in a significant change in the transcript levels. In plants treated with Bordeaux Mixture, the copper concentration increased twofold in the pulp and ninefold on the skin of surface-washed mature berries. Conclusions: The expression pattern of VvCTr1-8 depends on the tissue and developmental stage. Four applications of copper following a methodology used in organic farming are sufficient to induce a significant increase in copper content in berry tissues which is either a cause or a consequence of the observed transcriptional reprogramming of the expression of the VvCTrs. Significance of the Study: The application of Bordeaux Mixture should have major consequences on grapevine copper homeostasis, regulating copper transport at the transcriptional level. A new research avenue emerged towards the characterisation of VvCTr-mediated transport at the molecular level in response to copper.

  • metabolic changes of vitis vinifera berries and leaves exposed to Bordeaux Mixture
    Plant Physiology and Biochemistry, 2014
    Co-Authors: Viviana Martins, Antonio Teixeira, Elias Bassil, Eduardo Blumwald, Hernâni Geros
    Abstract:

    Since the development of Bordeaux Mixture in the late 1800's, copper-based fungicides have been widely used against grapevine (Vitis vinifera L.) diseases, mainly in organic but also in conventional viticulture; however their intensive use has raised phytotoxicity concerns. In this study, the composition of grape berries and leaves upon Bordeaux Mixture treatment was investigated during the fructification season by a metabolomic approach. Four applications of Bordeaux Mixture till 3 weeks before harvest were performed following the regular management practices of organic viticulture. Results showed that the copper-based treatment affected the content in sugars, organic acids, lipids and flavan-3-ols of grapes and leaves at specific developmental stages. Nonetheless, the levels of sucrose, glucose and fructose, and of tartaric and malic acids were not significantly affected in mature grapes. In contrast, a sharp decrease in free natural amino acids was observed, together with a reduction in protein content and in mineral nitrogen forms. The treatment with Bordeaux Mixture increased by 7-fold the copper levels in tissue extracts from surface-washed mature berries.