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Jean-michel Mérillon - One of the best experts on this subject based on the ideXlab platform.
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Anthocyanin phytochemical profiles and anti-oxidant activities of Vitis candicans and Vitis doaniana.
Phytochemical analysis : PCA, 2013Co-Authors: Alexander Acevedo De La Cruz, Céline Rivière, Jean Claude Delaunay, Louis Bordenave, Ghislaine Hilbert, Virginie Mengin, Nathalie Ollat, Caroline Vitrac, Stephane Decroocq, Jean-michel MérillonAbstract:Introduction Grapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American Vitis species have the greatest potential for breeding work. They have multiple resistance properties in comparison with Vitis vinifera but little is known about their anthocyanin content. Objective To determine the anti-oxidant properties and anthocyanin profile of two American species, Vitis candicans and Vitis doaniana, by using LC–MSn and LC–NMR. Methods Grape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC–MSn, LC–NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti-oxidant activities of grape skin extracts were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Results By using LC–MSn and LC–NMR experiments, 30 anthocyanins were identified and quantified in the two Vitis species, including two new cis-p-coumaroyl derivatives. Vitis candicans and V. doaniana showed significant differences in their anthocyanin profile. These two Vitis species possess low-to-medium anti-oxidant activities in comparison with V. vinifera. Conclusion The profiles of 30 anthocyanins were established unambiguously in two American Vitis species. Copyright © 2013 John Wiley & Sons, Ltd.
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Stilbenoid Profiles of Canes from Vitis and Muscadinia Species
Journal of agricultural and food chemistry, 2013Co-Authors: Alison D. Pawlus, Ramla Sahli, Jonathan Bisson, Céline Rivière, Jean Claude Delaunay, Tristan Richard, Eric Gomès, Louis Bordenave, Pierre Waffo-téguo, Jean-michel MérillonAbstract:We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [α](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis × champini, Vitis × doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V. vinifera × M. rotundifolia hybrid. In these species, E-ampelopsin E, E-amurensin B, E-piceid, E-piceatannol, E-resveratrol, E-resveratroloside, E-e-viniferin, E-ω-viniferin, and E-Vitisin B were quantified, when found in sufficient amounts. Total concentrations ranged from ~2.2 to 19.5 g/kg of dry weight. Additional stilbenoids, E-3,5,4'-trihydroxystilbene 2-C-glucoside, Z-ampelopsin E, Z-trans-miyabenol C, E-trans-miyabenol C, scirpusin A, and Z-Vitisin B, were identified but not quantified. Our results indicate that canes, particularly those of non-vinifera species, have substantial quantities of valuable, health-promoting stilbenoids.
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Stilbenoid profiles of canes from Vitis and Muscadinia species
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Alison D. Pawlus, Jonathan Bisson, Céline Rivière, Jean Claude Delaunay, Tristan Richard, Eric Gomès, Louis Bordenave, Pierre Waffo-téguo, Ramla Salhi, Jean-michel MérillonAbstract:We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [alpha](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis x champini, Vitis x doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V. vinifera x M. rotundifolia hybrid. In these species, E-ampelopsin E, E-amurensin B, E-piceid, E-piceatannol, E-resveratrol, E-resveratroloside, E-omega-viniferin, E-omega-viniferin, and E-Vitisin B were quantified, when found in sufficient amounts. Total concentrations ranged from similar to 2.2 to 19.5 g/kg of dry weight. Additional stilbenoids, E-3,5,4'-trihydroxystilbene 2-C-glucoside, Z-ampelopsin E, Z-trans-miyabenol C, E-trans-miyabenol C, scirpusin A, and Z-Vitisin B, were identified but not quantified. Our results indicate that canes, particularly those of non-vinifera species, have substantial quantities of valuable, health-promoting stilbenoids.
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Anthocyanin phytochemical profiles and anti-oxidant activities of Vitis candicans and Vitis doaniana
Phytochemical Analysis, 2013Co-Authors: Alexander Acevedo De La Cruz, Céline Rivière, Jean Claude Delaunay, Louis Bordenave, Ghislaine Hilbert, Virginie Mengin, Nathalie Ollat, Caroline Vitrac, Stephane Decroocq, Jean-michel MérillonAbstract:Introduction Grapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American Vitis species have the greatest potential for breeding work. They have multiple resistance properties in comparison with Vitis vinifera but little is known about their anthocyanin content. Objective To determine the anti-oxidant properties and anthocyanin profile of two American species, Vitis candicans and Vitis doaniana, by using LC–MSn and LC–NMR. Methods Grape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC–MSn, LC–NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti-oxidant activities of grape skin extracts were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Results By using LC–MSn and LC–NMR experiments, 30 anthocyanins were identified and quantified in the two Vitis species, including two new cis-p-coumaroyl derivatives. Vitis candicans and V. doaniana showed significant differences in their anthocyanin profile. These two Vitis species possess low-to-medium anti-oxidant activities in comparison with V. vinifera. Conclusion The profiles of 30 anthocyanins were established unambiguously in two American Vitis species.
Tristan Richard - One of the best experts on this subject based on the ideXlab platform.
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Polyphenol Profiles of Just Pruned Grapevine Canes from Wild Vitis Accessions and Vitis vinifera Cultivars
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Grégoire Loupit, Tristan Richard, Sylvain Prigent, Céline Franc, Gilles De Revel, Sarah Jane Cookson, Josep Valls FonayetAbstract:Grapevine canes are an abundant byproduct of the wine industry. The stilbene contents of Vitis vinifera cultivars have been largely studied, but little is known about the stilbene contents of wild Vitis accessions. Moreover, there have only been few studies on the quantification of other phenolic compounds in just pruned grapevine canes. In our study, we investigated the polyphenol profile of 51 genotypes belonging to 15 Vitis spp. A total of 36 polyphenols (20 stilbenes, 6 flavanols, 7 flavonols, and 3 phenolic acids) were analyzed by high-performance liquid chromatography coupled with a triple quadrupole mass spectrometer. Our results suggest that some wild Vitis accessions could be of interest in terms of the concentration of bioactive polyphenols and that flavanols contribute significantly to the antioxidant activity of grapevine cane extracts. To the best of our knowledge, this is the most exhaustive study of the polyphenolic composition of grapevine canes of wild Vitis spp.
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Triterpenoid profiles of the leaves of wild and domesticated grapevines
Phytochemistry Letters, 2019Co-Authors: Aleksandra Burdziej, Tristan Richard, Cezary Pączkowski, Agnes Destrac-irvine, Stéphanie Cluzet, Anna SzakielAbstract:The composition of steroids and triterpenoids in the leaves of eight grapevine species was analyzed in order to assess phenotypic differences. Four wild species (Vitis aestivalis Michx., Vitis labrusca L., Vitis riparia Michx., and Vitis vinifera subsp. sylvestris (Gmelin) Hegi) and four domesticated (Vitis vinifera cultivars: Cabernet Sauvignon, Gamay, Marselan, and Merlot) were studied. Although the main profiles of steroids and triterpenoids were similar in all analyzed extracts, remarkable quantitative differences in the content of these compounds in Vitis spp. leaves were demonstrated by GC–MS/FID analysis. The analyzed Vitis plants differed in the level of sterols, i.e. cholesterol (1), campesterol (2), stigmasterol (3), clionasterol (4) (exclusively in V. vinifera subsp. sylvestris (Gmelin) Hegi), sitosterol (5), and their amount was generally higher in wild grapevines. The predominance of pentacyclic triterpenoids of ursane-, oleanane-, lupane- and friedooleanane (taraxerane)-type skeletons depended on the studied cultivar/variety. Taraxerol (7) may be considered as a biomarker compound of V. vinifera leaves. The obtained data supplement information about biochemical diversity of Vitis genus and despite small qualitative differences allow species discrimination considering phylogenetic relationships confirmed by HCPC analysis. Differences between wild and cultivated grapevines in steroid and triterpenoid profiles may be connected to the domestication process.
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Stilbenoid Profiles of Canes from Vitis and Muscadinia Species
Journal of agricultural and food chemistry, 2013Co-Authors: Alison D. Pawlus, Ramla Sahli, Jonathan Bisson, Céline Rivière, Jean Claude Delaunay, Tristan Richard, Eric Gomès, Louis Bordenave, Pierre Waffo-téguo, Jean-michel MérillonAbstract:We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [α](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis × champini, Vitis × doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V. vinifera × M. rotundifolia hybrid. In these species, E-ampelopsin E, E-amurensin B, E-piceid, E-piceatannol, E-resveratrol, E-resveratroloside, E-e-viniferin, E-ω-viniferin, and E-Vitisin B were quantified, when found in sufficient amounts. Total concentrations ranged from ~2.2 to 19.5 g/kg of dry weight. Additional stilbenoids, E-3,5,4'-trihydroxystilbene 2-C-glucoside, Z-ampelopsin E, Z-trans-miyabenol C, E-trans-miyabenol C, scirpusin A, and Z-Vitisin B, were identified but not quantified. Our results indicate that canes, particularly those of non-vinifera species, have substantial quantities of valuable, health-promoting stilbenoids.
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Stilbenoid profiles of canes from Vitis and Muscadinia species
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Alison D. Pawlus, Jonathan Bisson, Céline Rivière, Jean Claude Delaunay, Tristan Richard, Eric Gomès, Louis Bordenave, Pierre Waffo-téguo, Ramla Salhi, Jean-michel MérillonAbstract:We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [alpha](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis x champini, Vitis x doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V. vinifera x M. rotundifolia hybrid. In these species, E-ampelopsin E, E-amurensin B, E-piceid, E-piceatannol, E-resveratrol, E-resveratroloside, E-omega-viniferin, E-omega-viniferin, and E-Vitisin B were quantified, when found in sufficient amounts. Total concentrations ranged from similar to 2.2 to 19.5 g/kg of dry weight. Additional stilbenoids, E-3,5,4'-trihydroxystilbene 2-C-glucoside, Z-ampelopsin E, Z-trans-miyabenol C, E-trans-miyabenol C, scirpusin A, and Z-Vitisin B, were identified but not quantified. Our results indicate that canes, particularly those of non-vinifera species, have substantial quantities of valuable, health-promoting stilbenoids.
Deborah A. Golino - One of the best experts on this subject based on the ideXlab platform.
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Vitis californica and Vitis californica × Vitis vinifera Hybrids are Hosts for Grapevine leafroll-associated virus-2 and -3 and Grapevine virus A and B.
Plant disease, 2011Co-Authors: Vicki Klaassen, S.t. Sim, G. S. Dangl, Fatima Osman, M. Al Rwahnih, Adib Rowhani, Deborah A. GolinoAbstract:Abstract Vitis and non-Vitis spp. surrounding nine Napa Valley vineyards were surveyed for Grapevine leafroll-associated virus (GLRaV)-1 to -5 and -9, Grapevine virus A (GVA), Grapevine virus B (GVB), and Grapevine virus D (GVD). Vitis spp. from three riparian areas not adjacent to vineyards were also included. DNA fingerprinting and probability analyses indicated that the Vitis samples consisted primarily of Vitis californica followed by V. californica × V. vinifera hybrids. Single and mixed infections of GLRaV-2, -3, GVA, or GVB were detected by conventional or quantitative reverse-transcription polymerase chain reaction in 6 of the 66 V. californica and 11 of the 19 V. californica × V. vinifera hybrids. GLRaV-1, -4, -5, -9, and GVD were not detected. Phylogenetic analysis of GLRaV-2 and -3 partial coat protein gene nucleotide sequences indicated that the isolates from V. californica and V. californica × V. vinifera hybrids were closely related to isolates from V. vinifera.
Louis Bordenave - One of the best experts on this subject based on the ideXlab platform.
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Anthocyanin phytochemical profiles and anti-oxidant activities of Vitis candicans and Vitis doaniana.
Phytochemical analysis : PCA, 2013Co-Authors: Alexander Acevedo De La Cruz, Céline Rivière, Jean Claude Delaunay, Louis Bordenave, Ghislaine Hilbert, Virginie Mengin, Nathalie Ollat, Caroline Vitrac, Stephane Decroocq, Jean-michel MérillonAbstract:Introduction Grapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American Vitis species have the greatest potential for breeding work. They have multiple resistance properties in comparison with Vitis vinifera but little is known about their anthocyanin content. Objective To determine the anti-oxidant properties and anthocyanin profile of two American species, Vitis candicans and Vitis doaniana, by using LC–MSn and LC–NMR. Methods Grape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC–MSn, LC–NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti-oxidant activities of grape skin extracts were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Results By using LC–MSn and LC–NMR experiments, 30 anthocyanins were identified and quantified in the two Vitis species, including two new cis-p-coumaroyl derivatives. Vitis candicans and V. doaniana showed significant differences in their anthocyanin profile. These two Vitis species possess low-to-medium anti-oxidant activities in comparison with V. vinifera. Conclusion The profiles of 30 anthocyanins were established unambiguously in two American Vitis species. Copyright © 2013 John Wiley & Sons, Ltd.
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Stilbenoid Profiles of Canes from Vitis and Muscadinia Species
Journal of agricultural and food chemistry, 2013Co-Authors: Alison D. Pawlus, Ramla Sahli, Jonathan Bisson, Céline Rivière, Jean Claude Delaunay, Tristan Richard, Eric Gomès, Louis Bordenave, Pierre Waffo-téguo, Jean-michel MérillonAbstract:We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [α](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis × champini, Vitis × doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V. vinifera × M. rotundifolia hybrid. In these species, E-ampelopsin E, E-amurensin B, E-piceid, E-piceatannol, E-resveratrol, E-resveratroloside, E-e-viniferin, E-ω-viniferin, and E-Vitisin B were quantified, when found in sufficient amounts. Total concentrations ranged from ~2.2 to 19.5 g/kg of dry weight. Additional stilbenoids, E-3,5,4'-trihydroxystilbene 2-C-glucoside, Z-ampelopsin E, Z-trans-miyabenol C, E-trans-miyabenol C, scirpusin A, and Z-Vitisin B, were identified but not quantified. Our results indicate that canes, particularly those of non-vinifera species, have substantial quantities of valuable, health-promoting stilbenoids.
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Stilbenoid profiles of canes from Vitis and Muscadinia species
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Alison D. Pawlus, Jonathan Bisson, Céline Rivière, Jean Claude Delaunay, Tristan Richard, Eric Gomès, Louis Bordenave, Pierre Waffo-téguo, Ramla Salhi, Jean-michel MérillonAbstract:We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [alpha](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis x champini, Vitis x doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V. vinifera x M. rotundifolia hybrid. In these species, E-ampelopsin E, E-amurensin B, E-piceid, E-piceatannol, E-resveratrol, E-resveratroloside, E-omega-viniferin, E-omega-viniferin, and E-Vitisin B were quantified, when found in sufficient amounts. Total concentrations ranged from similar to 2.2 to 19.5 g/kg of dry weight. Additional stilbenoids, E-3,5,4'-trihydroxystilbene 2-C-glucoside, Z-ampelopsin E, Z-trans-miyabenol C, E-trans-miyabenol C, scirpusin A, and Z-Vitisin B, were identified but not quantified. Our results indicate that canes, particularly those of non-vinifera species, have substantial quantities of valuable, health-promoting stilbenoids.
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Anthocyanin phytochemical profiles and anti-oxidant activities of Vitis candicans and Vitis doaniana
Phytochemical Analysis, 2013Co-Authors: Alexander Acevedo De La Cruz, Céline Rivière, Jean Claude Delaunay, Louis Bordenave, Ghislaine Hilbert, Virginie Mengin, Nathalie Ollat, Caroline Vitrac, Stephane Decroocq, Jean-michel MérillonAbstract:Introduction Grapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American Vitis species have the greatest potential for breeding work. They have multiple resistance properties in comparison with Vitis vinifera but little is known about their anthocyanin content. Objective To determine the anti-oxidant properties and anthocyanin profile of two American species, Vitis candicans and Vitis doaniana, by using LC–MSn and LC–NMR. Methods Grape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC–MSn, LC–NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti-oxidant activities of grape skin extracts were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Results By using LC–MSn and LC–NMR experiments, 30 anthocyanins were identified and quantified in the two Vitis species, including two new cis-p-coumaroyl derivatives. Vitis candicans and V. doaniana showed significant differences in their anthocyanin profile. These two Vitis species possess low-to-medium anti-oxidant activities in comparison with V. vinifera. Conclusion The profiles of 30 anthocyanins were established unambiguously in two American Vitis species.
Céline Rivière - One of the best experts on this subject based on the ideXlab platform.
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Anthocyanin phytochemical profiles and anti-oxidant activities of Vitis candicans and Vitis doaniana.
Phytochemical analysis : PCA, 2013Co-Authors: Alexander Acevedo De La Cruz, Céline Rivière, Jean Claude Delaunay, Louis Bordenave, Ghislaine Hilbert, Virginie Mengin, Nathalie Ollat, Caroline Vitrac, Stephane Decroocq, Jean-michel MérillonAbstract:Introduction Grapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American Vitis species have the greatest potential for breeding work. They have multiple resistance properties in comparison with Vitis vinifera but little is known about their anthocyanin content. Objective To determine the anti-oxidant properties and anthocyanin profile of two American species, Vitis candicans and Vitis doaniana, by using LC–MSn and LC–NMR. Methods Grape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC–MSn, LC–NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti-oxidant activities of grape skin extracts were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Results By using LC–MSn and LC–NMR experiments, 30 anthocyanins were identified and quantified in the two Vitis species, including two new cis-p-coumaroyl derivatives. Vitis candicans and V. doaniana showed significant differences in their anthocyanin profile. These two Vitis species possess low-to-medium anti-oxidant activities in comparison with V. vinifera. Conclusion The profiles of 30 anthocyanins were established unambiguously in two American Vitis species. Copyright © 2013 John Wiley & Sons, Ltd.
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Stilbenoid Profiles of Canes from Vitis and Muscadinia Species
Journal of agricultural and food chemistry, 2013Co-Authors: Alison D. Pawlus, Ramla Sahli, Jonathan Bisson, Céline Rivière, Jean Claude Delaunay, Tristan Richard, Eric Gomès, Louis Bordenave, Pierre Waffo-téguo, Jean-michel MérillonAbstract:We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [α](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis × champini, Vitis × doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V. vinifera × M. rotundifolia hybrid. In these species, E-ampelopsin E, E-amurensin B, E-piceid, E-piceatannol, E-resveratrol, E-resveratroloside, E-e-viniferin, E-ω-viniferin, and E-Vitisin B were quantified, when found in sufficient amounts. Total concentrations ranged from ~2.2 to 19.5 g/kg of dry weight. Additional stilbenoids, E-3,5,4'-trihydroxystilbene 2-C-glucoside, Z-ampelopsin E, Z-trans-miyabenol C, E-trans-miyabenol C, scirpusin A, and Z-Vitisin B, were identified but not quantified. Our results indicate that canes, particularly those of non-vinifera species, have substantial quantities of valuable, health-promoting stilbenoids.
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Stilbenoid profiles of canes from Vitis and Muscadinia species
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Alison D. Pawlus, Jonathan Bisson, Céline Rivière, Jean Claude Delaunay, Tristan Richard, Eric Gomès, Louis Bordenave, Pierre Waffo-téguo, Ramla Salhi, Jean-michel MérillonAbstract:We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [alpha](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis x champini, Vitis x doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V. vinifera x M. rotundifolia hybrid. In these species, E-ampelopsin E, E-amurensin B, E-piceid, E-piceatannol, E-resveratrol, E-resveratroloside, E-omega-viniferin, E-omega-viniferin, and E-Vitisin B were quantified, when found in sufficient amounts. Total concentrations ranged from similar to 2.2 to 19.5 g/kg of dry weight. Additional stilbenoids, E-3,5,4'-trihydroxystilbene 2-C-glucoside, Z-ampelopsin E, Z-trans-miyabenol C, E-trans-miyabenol C, scirpusin A, and Z-Vitisin B, were identified but not quantified. Our results indicate that canes, particularly those of non-vinifera species, have substantial quantities of valuable, health-promoting stilbenoids.
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Anthocyanin phytochemical profiles and anti-oxidant activities of Vitis candicans and Vitis doaniana
Phytochemical Analysis, 2013Co-Authors: Alexander Acevedo De La Cruz, Céline Rivière, Jean Claude Delaunay, Louis Bordenave, Ghislaine Hilbert, Virginie Mengin, Nathalie Ollat, Caroline Vitrac, Stephane Decroocq, Jean-michel MérillonAbstract:Introduction Grapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American Vitis species have the greatest potential for breeding work. They have multiple resistance properties in comparison with Vitis vinifera but little is known about their anthocyanin content. Objective To determine the anti-oxidant properties and anthocyanin profile of two American species, Vitis candicans and Vitis doaniana, by using LC–MSn and LC–NMR. Methods Grape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC–MSn, LC–NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti-oxidant activities of grape skin extracts were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Results By using LC–MSn and LC–NMR experiments, 30 anthocyanins were identified and quantified in the two Vitis species, including two new cis-p-coumaroyl derivatives. Vitis candicans and V. doaniana showed significant differences in their anthocyanin profile. These two Vitis species possess low-to-medium anti-oxidant activities in comparison with V. vinifera. Conclusion The profiles of 30 anthocyanins were established unambiguously in two American Vitis species.
