The Experts below are selected from a list of 174 Experts worldwide ranked by ideXlab platform

Cécile Clercx - One of the best experts on this subject based on the ideXlab platform.

  • Comparison of C-reactive protein concentrations in dogs with Bordetella Bronchiseptica Infection and aspiration bronchopneumonia.
    Journal of veterinary internal medicine, 2021
    Co-Authors: Aude Morgane Canonne, Frédéric Billen, Maud Menard, Christelle Maurey, Ghita Benchrekroun, Nina Fernandes Rodrigues, Cécile Clercx
    Abstract:

    BACKGROUND C-reactive protein (CRP) is a well-known acute-phase protein in dogs that may discriminate bacterial bronchopneumonia from other pulmonary conditions. Bronchopneumonia caused by Bordetella Bronchiseptica (Bb) is common but the associated increase in CRP concentration in naturally infected dogs has not been fully explored. OBJECTIVE To compare CRP concentrations of dogs with Bb Infection, with or without radiographic pulmonary lesions, to dogs with aspiration bronchopneumonia (ABP). ANIMALS Sixteen dogs with Bb Infection and 36 dogs with ABP. METHODS Retrospective study. C-reactive protein concentrations and thoracic radiographs were available for each dog. RESULTS Eleven dogs with Bb Infection had alveolar lesions. In all dogs, CRP concentration was mildly increased (14-38 mg/L). In the 5 dogs without alveolar lesions, CRP concentration was within the reference range in all but 1 dog, in which it was slightly increased. Median CRP concentration was significantly higher in dogs with alveolar lesions (20 mg/L) compared with dogs without alveolar lesions (5 mg/L; p < .002). In dogs with Bb Infection, median duration of clinical signs was not different between dogs with normal CRP concentration and dogs with increased concentration. In dogs with Bb Infection either with or without alveolar lessions, median CRP concentration was significantly lower (20 mg/L) than in dogs with ABP (118 mg/L; p < .001). CONCLUSIONS AND CLINICAL IMPORTANCE In contrast to dogs with APB, CRP was not a good marker for the diagnosis of dogs suspected to have bordetellosis. Confirmation of Bb Infection still requires lower airway sampling.

  • Clinical response to 2 protocols of aerosolized gentamicin in 46 dogs with Bordetella Bronchiseptica Infection (2012-2018).
    Journal of veterinary internal medicine, 2020
    Co-Authors: Aude Morgane Canonne, Frédéric Billen, Elodie Roels, Maud Menard, Loïc Desquilbet, Cécile Clercx
    Abstract:

    Background Bordetella Bronchiseptica (Bb) Infection commonly causes respiratory disease in dogs. Gentamicin delivered by aerosol maximizes local drug delivery without systemic absorption but clinical response to protocols remains undetermined. Objectives To compare the clinical response to 2 protocols of aerosolized delivery of gentamicin in bordetellosis. Animals Forty-six dogs with Bb Infection confirmed by culture or quantitative polymerase chain reaction on bronchoalveolar lavage. Methods Retrospective study. Administration of aerosolized gentamicin for ≥10 minutes q12h for ≥3 weeks using 4 mg/kg diluted with saline (group 1) or undiluted 5% solution (group 2). Clinical response firstly assessed after 3-4 weeks and treatment pursued by 3-weeks increments if cure not reached. Cure defined as absence of cough persisting at least a week after treatment interruption. Results Demographic data were similar between both groups. Clinical cure at 3-4 weeks was more frequently observed with the use of undiluted solution (19/33 vs 3/13 dogs, P = .03) in association with a shorter median duration of treatment (4 vs 6 weeks, P = .01). Dogs from group 2 having less than 1000 cells/μL in lavage were also more likely to be cured at 3-4 weeks than dogs with more than 1000 cells/μL [9/9 vs 10/19, P = .006] and median duration of treatment in that subgroup of animals was reduced (3 vs 5 weeks, P = .02). Conclusion and clinical importance Aerosolized delivery of gentamicin seems effective for inducing clinical cure in Bb Infection. Clinical response appears better using undiluted 5% solution, particularly in the subgroup of dogs having less than 1000 cells/μL in lavage.

  • Analysis of the lung microbiota in dogs with Bordetella Bronchiseptica Infection and correlation with culture and quantitative polymerase chain reaction
    Veterinary Research, 2020
    Co-Authors: Aline Fastrès, Frédéric Billen, Morgane A. Canonne, Bernard Taminiau, Mutien-marie Garigliany, Georges Daube, Cécile Clercx
    Abstract:

    Infection with Bordetella Bronchiseptica ( Bb ), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung Infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos ( Mc ) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bb in all diseased dogs, about half being co-infected with Mc . In diseased compared with healthy dogs, the β-diversity changed ( P  = 0.0024); bacterial richness and α-diversity were lower ( P  = 0.012 and 0.0061), and bacterial load higher ( P  = 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P  

  • Quantitative PCR and Cytology of Bronchoalveolar Lavage Fluid in Dogs with Bordetella Bronchiseptica Infection.
    Journal of veterinary internal medicine, 2016
    Co-Authors: A.m. Canonne, Frédéric Billen, Charlotte Tual, Eve Ramery, Elodie Roels, Iain R. Peters, Cécile Clercx
    Abstract:

    Background The use of quantitative PCR (qPCR) for detection of Bordetella Bronchiseptica in bronchoalveolar lavage fluid (BALF) and demonstration of bacteria adhering to ciliated epithelial cells in BALF or bronchial brushing fluid (BBF) has not been assessed in a series of affected dogs. CoInfections can worsen the clinical severity in bordetellosis, but the specific association with Mycoplasma cynos has not been evaluated. Objectives To assess the utility of culture, qPCR and cytologic examination of cytospin preparations in the diagnosis of bordetellosis in dogs and the influence of coInfection by M. cynos on disease severity. Animals Twenty-four referred dogs with B. Bronchiseptica Infection and 10 healthy dogs. Methods Retrospective case series. qPCR (B. Bronchiseptica and M. cynos) and culture results from BALF were recorded. Cytospin preparations from BALF and BBF were reviewed. qPCR on BALF from 10 healthy dogs were used as negative control. Results The BALF culture and qPCR detected B. Bronchiseptica in 14/24 and 18/18 dogs, respectively. Coccobacilli were found adhering to ciliated epithelial cells in 20 of the 21 BALF cytologic preparations where epithelial cells were found, and 2/3 BBF cytologic preparations. Quantitative PCR detected a low level of B. Bronchiseptica in one healthy dog. The frequency of detection of M. cynos was not significantly different in B. Bronchiseptica (9/17 dogs) compared with healthy dogs (2/10 dogs) (P = .09). Conclusion and Clinical Importance Quantitative PCR detection of B. Bronchiseptica in BALF appears to be a useful diagnostic tool. Cytologic examination of BALF or BBF, when positive, allows a rapid and reliable diagnosis.

Frédéric Billen - One of the best experts on this subject based on the ideXlab platform.

  • Comparison of C-reactive protein concentrations in dogs with Bordetella Bronchiseptica Infection and aspiration bronchopneumonia.
    Journal of veterinary internal medicine, 2021
    Co-Authors: Aude Morgane Canonne, Frédéric Billen, Maud Menard, Christelle Maurey, Ghita Benchrekroun, Nina Fernandes Rodrigues, Cécile Clercx
    Abstract:

    BACKGROUND C-reactive protein (CRP) is a well-known acute-phase protein in dogs that may discriminate bacterial bronchopneumonia from other pulmonary conditions. Bronchopneumonia caused by Bordetella Bronchiseptica (Bb) is common but the associated increase in CRP concentration in naturally infected dogs has not been fully explored. OBJECTIVE To compare CRP concentrations of dogs with Bb Infection, with or without radiographic pulmonary lesions, to dogs with aspiration bronchopneumonia (ABP). ANIMALS Sixteen dogs with Bb Infection and 36 dogs with ABP. METHODS Retrospective study. C-reactive protein concentrations and thoracic radiographs were available for each dog. RESULTS Eleven dogs with Bb Infection had alveolar lesions. In all dogs, CRP concentration was mildly increased (14-38 mg/L). In the 5 dogs without alveolar lesions, CRP concentration was within the reference range in all but 1 dog, in which it was slightly increased. Median CRP concentration was significantly higher in dogs with alveolar lesions (20 mg/L) compared with dogs without alveolar lesions (5 mg/L; p < .002). In dogs with Bb Infection, median duration of clinical signs was not different between dogs with normal CRP concentration and dogs with increased concentration. In dogs with Bb Infection either with or without alveolar lessions, median CRP concentration was significantly lower (20 mg/L) than in dogs with ABP (118 mg/L; p < .001). CONCLUSIONS AND CLINICAL IMPORTANCE In contrast to dogs with APB, CRP was not a good marker for the diagnosis of dogs suspected to have bordetellosis. Confirmation of Bb Infection still requires lower airway sampling.

  • Clinical response to 2 protocols of aerosolized gentamicin in 46 dogs with Bordetella Bronchiseptica Infection (2012-2018).
    Journal of veterinary internal medicine, 2020
    Co-Authors: Aude Morgane Canonne, Frédéric Billen, Elodie Roels, Maud Menard, Loïc Desquilbet, Cécile Clercx
    Abstract:

    Background Bordetella Bronchiseptica (Bb) Infection commonly causes respiratory disease in dogs. Gentamicin delivered by aerosol maximizes local drug delivery without systemic absorption but clinical response to protocols remains undetermined. Objectives To compare the clinical response to 2 protocols of aerosolized delivery of gentamicin in bordetellosis. Animals Forty-six dogs with Bb Infection confirmed by culture or quantitative polymerase chain reaction on bronchoalveolar lavage. Methods Retrospective study. Administration of aerosolized gentamicin for ≥10 minutes q12h for ≥3 weeks using 4 mg/kg diluted with saline (group 1) or undiluted 5% solution (group 2). Clinical response firstly assessed after 3-4 weeks and treatment pursued by 3-weeks increments if cure not reached. Cure defined as absence of cough persisting at least a week after treatment interruption. Results Demographic data were similar between both groups. Clinical cure at 3-4 weeks was more frequently observed with the use of undiluted solution (19/33 vs 3/13 dogs, P = .03) in association with a shorter median duration of treatment (4 vs 6 weeks, P = .01). Dogs from group 2 having less than 1000 cells/μL in lavage were also more likely to be cured at 3-4 weeks than dogs with more than 1000 cells/μL [9/9 vs 10/19, P = .006] and median duration of treatment in that subgroup of animals was reduced (3 vs 5 weeks, P = .02). Conclusion and clinical importance Aerosolized delivery of gentamicin seems effective for inducing clinical cure in Bb Infection. Clinical response appears better using undiluted 5% solution, particularly in the subgroup of dogs having less than 1000 cells/μL in lavage.

  • Analysis of the lung microbiota in dogs with Bordetella Bronchiseptica Infection and correlation with culture and quantitative polymerase chain reaction
    Veterinary Research, 2020
    Co-Authors: Aline Fastrès, Frédéric Billen, Morgane A. Canonne, Bernard Taminiau, Mutien-marie Garigliany, Georges Daube, Cécile Clercx
    Abstract:

    Infection with Bordetella Bronchiseptica ( Bb ), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung Infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos ( Mc ) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bb in all diseased dogs, about half being co-infected with Mc . In diseased compared with healthy dogs, the β-diversity changed ( P  = 0.0024); bacterial richness and α-diversity were lower ( P  = 0.012 and 0.0061), and bacterial load higher ( P  = 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P  

  • Quantitative PCR and Cytology of Bronchoalveolar Lavage Fluid in Dogs with Bordetella Bronchiseptica Infection.
    Journal of veterinary internal medicine, 2016
    Co-Authors: A.m. Canonne, Frédéric Billen, Charlotte Tual, Eve Ramery, Elodie Roels, Iain R. Peters, Cécile Clercx
    Abstract:

    Background The use of quantitative PCR (qPCR) for detection of Bordetella Bronchiseptica in bronchoalveolar lavage fluid (BALF) and demonstration of bacteria adhering to ciliated epithelial cells in BALF or bronchial brushing fluid (BBF) has not been assessed in a series of affected dogs. CoInfections can worsen the clinical severity in bordetellosis, but the specific association with Mycoplasma cynos has not been evaluated. Objectives To assess the utility of culture, qPCR and cytologic examination of cytospin preparations in the diagnosis of bordetellosis in dogs and the influence of coInfection by M. cynos on disease severity. Animals Twenty-four referred dogs with B. Bronchiseptica Infection and 10 healthy dogs. Methods Retrospective case series. qPCR (B. Bronchiseptica and M. cynos) and culture results from BALF were recorded. Cytospin preparations from BALF and BBF were reviewed. qPCR on BALF from 10 healthy dogs were used as negative control. Results The BALF culture and qPCR detected B. Bronchiseptica in 14/24 and 18/18 dogs, respectively. Coccobacilli were found adhering to ciliated epithelial cells in 20 of the 21 BALF cytologic preparations where epithelial cells were found, and 2/3 BBF cytologic preparations. Quantitative PCR detected a low level of B. Bronchiseptica in one healthy dog. The frequency of detection of M. cynos was not significantly different in B. Bronchiseptica (9/17 dogs) compared with healthy dogs (2/10 dogs) (P = .09). Conclusion and Clinical Importance Quantitative PCR detection of B. Bronchiseptica in BALF appears to be a useful diagnostic tool. Cytologic examination of BALF or BBF, when positive, allows a rapid and reliable diagnosis.

Clercx Cécile - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of the lung microbiota in dogs with Bordetella Bronchiseptica Infection and correlation with culture and quantitative polymerase chain reaction
    2020
    Co-Authors: Fastrès Aline, Canonne-guibert Morgane, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Clercx Cécile
    Abstract:

    Infection with Bordetella Bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung Infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bbin all diseased dogs, about half being co‑infected with Mc. In diseased compared with healthy dogs, the β‑diversity changed (P=0.0024); bacterial richness and α‑diversity were lower (P=0.012 and 0.0061), and bacterial load higher (P= 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P= 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb Infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases.Peer reviewe

  • Analysis of the lung microbiota in dogs with Bordetella Bronchiseptica Infection and correlation with culture and quantitative polymerase chain reaction
    'Springer Science and Business Media LLC', 2020
    Co-Authors: Fastrès Aline, Canonne-guibert Morgane, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Clercx Cécile
    Abstract:

    peer reviewedaudience: researcher, professional, studentInfection with Bordetella Bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung Infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bbin all diseased dogs, about half being co‑infected with Mc. In diseased compared with healthy dogs, the β‑diversity changed (P=0.0024); bacterial richness and α‑diversity were lower (P=0.012 and 0.0061), and bacterial load higher (P= 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P= 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb Infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases

  • Analysis of the lung microbiota in dogs with Bordetella Bronchiseptica Infection and correlation with culture and quantitative polymerase chain reaction
    'Springer Science and Business Media LLC', 2020
    Co-Authors: Fastrès Aline, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Canonne, Morgane A., Clercx Cécile
    Abstract:

    Infection with Bordetella Bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung Infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bb in all diseased dogs, about half being co-infected with Mc. In diseased compared with healthy dogs, the β-diversity changed (P = 0.0024); bacterial richness and α-diversity were lower (P = 0.012 and 0.0061), and bacterial load higher (P = 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P = 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb Infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases

  • ANALYSIS OF THE LUNG MICROBIOTA IN DOGS WITH Bordetella Bronchiseptica Infection AND CORRELATION WITH CULTURE AND QUANTITATIVE POLYMERASE CHAIN REACTION
    2019
    Co-Authors: Fastrès Aline, Canonne-guibert Morgane, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Clercx Cécile
    Abstract:

    Purpose of the study: Infection with Bordetella Bronchiseptica, one of the principal pathogens involved in canine infectious respiratory disease complex (CIRD-C), can be diagnosed using bacterial culture or quantitative polymerase chain reaction (qPCR). The lung microbiota (LM) has been described in healthy experimental dogs, while it has not yet been studied in dogs with lower respiratory Infection. In the present study we aimed to analyze the LM in dogs with B. Bronchiseptica Infection in comparison with healthy dogs, and to assess the correlation between 16S rDNA amplicon sequencing and culture and qPCR/ and to correlate 16S rDNA amplicon sequencing with culture and qPCR results. Methods used: Twenty dogs with B. Bronchiseptica Infection and 4 healthy age-matched dogs were retrospectively included. B. Bronchiseptica Infection was diagnosed based on either positive qPCR (n=19/19) and/or positive culture (n=11/17, including 1 dog with culture available only) in the bronchoalveolar lavage fluid (BALF). qPCR for Mycoplasma cynos were also available in 18 of the dogs with B. Bronchiseptica Infection and the 4 healthy dogs. qPCRs results were categorized into 6 classes based on the cycle threshold values. 16S rDNA sequences were obtained from naïve BALF after DNA extraction, PCR targeting the V1-V3 region of the 16S rDNA and sequencing. Sequences were mapped on the SILVA database with an OTU clustering distance of 0.03. Total bacterial load was calculated with a qPCR targeting the V2-V3 region of the 16S rDNA. Analysis of the LM were performed using MOTHUR v1.39 for the ecological data calculation including the β and α-diversity, the richness and the evenness. Mann-Whitney tests were used to compare the ecological data and the bacterial load. Welsh t-tests and FDR correction were used to compare the differences in relative abundances. Correlations between sequencing results at each taxonomic level and qPCRs or culture were done by Spearman tests. Summary of the results: 16S rDNA sequencing results showed the presence of B. Bronchiseptica in large amounts in all BALF samples in diseased dogs. Seven of them were co-infected with M. cynos, one dog with another species of Mycoplasma, and one with bacteria of the Pseudomonas genus. A shift in the β-diversity of the LM was observed in diseased compared with healthy dogs (P=0.002). Compared with healthy dogs, there were significantly more Burkholderiaceae at family level and Bordetella at genus level (75.92 ± 25.99 versus 2.94 ± 2.56%; P=0.058 and 75.65 ± 26.04 versus 0.11 ± 0.19%; P=0.10 respectively) in diseased dogs. The richness and the α-diversity were significantly lower (93.09 ± 76.19 versus 340.50 ± 158.68; P=0.012 and 1.54 ± 0.48 versus 17.74 ± 9.36; P=0.006 respectively) and the bacterial load higher (5.72 ± 0.38 versus 4.93 ± 0.15; P=0.004) in diseased compared with healthy dogs. B. Bronchiseptica qPCR classes and positive culture results positively correlated with the relative abundance of B. Bronchiseptica obtained by 16S rDNA amplicon sequencing (r= 0.56, P=0.028 and r=0.70, P=0.002 respectively). M. cynos qPCR classes also positively correlated with the relative abundance of M. cynos (r=0.84, P

  • ANALYSIS OF THE LUNG MICROBIOTA IN DOGS WITH Bordetella Bronchiseptica Infection AND CORRELATION WITH CULTURE AND QUANTITATIVE POLYMERASE CHAIN REACTION
    2019
    Co-Authors: Fastrès Aline, Canonne-guibert Morgane, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Clercx Cécile
    Abstract:

    audience: researcher, professional, studentPurpose of the study: Infection with Bordetella Bronchiseptica, one of the principal pathogens involved in canine infectious respiratory disease complex (CIRD-C), can be diagnosed using bacterial culture or quantitative polymerase chain reaction (qPCR). The lung microbiota (LM) has been described in healthy experimental dogs, while it has not yet been studied in dogs with lower respiratory Infection. In the present study we aimed to analyze the LM in dogs with B. Bronchiseptica Infection in comparison with healthy dogs, and to assess the correlation between 16S rDNA amplicon sequencing and culture and qPCR/ and to correlate 16S rDNA amplicon sequencing with culture and qPCR results. Methods used: Twenty dogs with B. Bronchiseptica Infection and 4 healthy age-matched dogs were retrospectively included. B. Bronchiseptica Infection was diagnosed based on either positive qPCR (n=19/19) and/or positive culture (n=11/17, including 1 dog with culture available only) in the bronchoalveolar lavage fluid (BALF). qPCR for Mycoplasma cynos were also available in 18 of the dogs with B. Bronchiseptica Infection and the 4 healthy dogs. qPCRs results were categorized into 6 classes based on the cycle threshold values. 16S rDNA sequences were obtained from naïve BALF after DNA extraction, PCR targeting the V1-V3 region of the 16S rDNA and sequencing. Sequences were mapped on the SILVA database with an OTU clustering distance of 0.03. Total bacterial load was calculated with a qPCR targeting the V2-V3 region of the 16S rDNA. Analysis of the LM were performed using MOTHUR v1.39 for the ecological data calculation including the β and α-diversity, the richness and the evenness. Mann-Whitney tests were used to compare the ecological data and the bacterial load. Welsh t-tests and FDR correction were used to compare the differences in relative abundances. Correlations between sequencing results at each taxonomic level and qPCRs or culture were done by Spearman tests. Summary of the results: 16S rDNA sequencing results showed the presence of B. Bronchiseptica in large amounts in all BALF samples in diseased dogs. Seven of them were co-infected with M. cynos, one dog with another species of Mycoplasma, and one with bacteria of the Pseudomonas genus. A shift in the β-diversity of the LM was observed in diseased compared with healthy dogs (P=0.002). Compared with healthy dogs, there were significantly more Burkholderiaceae at family level and Bordetella at genus level (75.92 ± 25.99 versus 2.94 ± 2.56%; P=0.058 and 75.65 ± 26.04 versus 0.11 ± 0.19%; P=0.10 respectively) in diseased dogs. The richness and the α-diversity were significantly lower (93.09 ± 76.19 versus 340.50 ± 158.68; P=0.012 and 1.54 ± 0.48 versus 17.74 ± 9.36; P=0.006 respectively) and the bacterial load higher (5.72 ± 0.38 versus 4.93 ± 0.15; P=0.004) in diseased compared with healthy dogs. B. Bronchiseptica qPCR classes and positive culture results positively correlated with the relative abundance of B. Bronchiseptica obtained by 16S rDNA amplicon sequencing (r= 0.56, P=0.028 and r=0.70, P=0.002 respectively). M. cynos qPCR classes also positively correlated with the relative abundance of M. cynos (r=0.84, P

Yasuhiko Horiguchi - One of the best experts on this subject based on the ideXlab platform.

  • Association of Bordetella dermonecrotic toxin with the extracellular matrix.
    BMC microbiology, 2010
    Co-Authors: Aya Fukui-miyazaki, Shigeki Kamitani, Masami Miyake, Yasuhiko Horiguchi
    Abstract:

    Background Bordetella dermonecrotic toxin (DNT) causes the turbinate atrophy in swine atrophic rhinitis, caused by a Bordetella Bronchiseptica Infection of pigs, by inhibiting osteoblastic differentiation. The toxin is not actively secreted from the bacteria, and is presumed to be present in only small amounts in infected areas. How such small amounts can affect target tissues is unknown.

  • Association of Bordetella dermonecrotic toxin with the extracellular matrix
    BMC Microbiology, 2010
    Co-Authors: Aya Fukui-miyazaki, Shigeki Kamitani, Masami Miyake, Yasuhiko Horiguchi
    Abstract:

    Background Bordetella dermonecrotic toxin (DNT) causes the turbinate atrophy in swine atrophic rhinitis, caused by a Bordetella Bronchiseptica Infection of pigs, by inhibiting osteoblastic differentiation. The toxin is not actively secreted from the bacteria, and is presumed to be present in only small amounts in infected areas. How such small amounts can affect target tissues is unknown. Results Fluorescence microscopy revealed that DNT associated with a fibrillar structure developed on cultured cells. A cellular component cross-linked with DNT conjugated with a cross-linker was identified as fibronectin by mass spectrometry. Colocalization of the fibronectin network on the cells with DNT was also observed by fluorescence microscope. Several lines of evidence suggested that DNT interacts with fibronectin not directly, but through another cellular component that remains to be identified. The colocalization was observed in not only DNT-sensitive cells but also insensitive cells, indicating that the fibronectin network neither serves as a receptor for the toxin nor is involved in the intoxicating procedures. The fibronectin network-associated toxin was easily liberated when the concentration of toxin in the local environment decreased, and was still active. Conclusions Components in the extracellular matrix are known to regulate activities of various growth factors by binding and liberating them in response to alterations in the extracellular environment. Similarly, the fibronectin-based extracellular matrix may function as a temporary storage system for DNT, enabling small amounts of the toxin to efficiently affect target tissues or cells.

Fastrès Aline - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of the lung microbiota in dogs with Bordetella Bronchiseptica Infection and correlation with culture and quantitative polymerase chain reaction
    2020
    Co-Authors: Fastrès Aline, Canonne-guibert Morgane, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Clercx Cécile
    Abstract:

    Infection with Bordetella Bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung Infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bbin all diseased dogs, about half being co‑infected with Mc. In diseased compared with healthy dogs, the β‑diversity changed (P=0.0024); bacterial richness and α‑diversity were lower (P=0.012 and 0.0061), and bacterial load higher (P= 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P= 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb Infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases.Peer reviewe

  • Analysis of the lung microbiota in dogs with Bordetella Bronchiseptica Infection and correlation with culture and quantitative polymerase chain reaction
    'Springer Science and Business Media LLC', 2020
    Co-Authors: Fastrès Aline, Canonne-guibert Morgane, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Clercx Cécile
    Abstract:

    peer reviewedaudience: researcher, professional, studentInfection with Bordetella Bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung Infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bbin all diseased dogs, about half being co‑infected with Mc. In diseased compared with healthy dogs, the β‑diversity changed (P=0.0024); bacterial richness and α‑diversity were lower (P=0.012 and 0.0061), and bacterial load higher (P= 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P= 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb Infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases

  • Analysis of the lung microbiota in dogs with Bordetella Bronchiseptica Infection and correlation with culture and quantitative polymerase chain reaction
    'Springer Science and Business Media LLC', 2020
    Co-Authors: Fastrès Aline, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Canonne, Morgane A., Clercx Cécile
    Abstract:

    Infection with Bordetella Bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung Infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bb in all diseased dogs, about half being co-infected with Mc. In diseased compared with healthy dogs, the β-diversity changed (P = 0.0024); bacterial richness and α-diversity were lower (P = 0.012 and 0.0061), and bacterial load higher (P = 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P = 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb Infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases

  • ANALYSIS OF THE LUNG MICROBIOTA IN DOGS WITH Bordetella Bronchiseptica Infection AND CORRELATION WITH CULTURE AND QUANTITATIVE POLYMERASE CHAIN REACTION
    2019
    Co-Authors: Fastrès Aline, Canonne-guibert Morgane, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Clercx Cécile
    Abstract:

    Purpose of the study: Infection with Bordetella Bronchiseptica, one of the principal pathogens involved in canine infectious respiratory disease complex (CIRD-C), can be diagnosed using bacterial culture or quantitative polymerase chain reaction (qPCR). The lung microbiota (LM) has been described in healthy experimental dogs, while it has not yet been studied in dogs with lower respiratory Infection. In the present study we aimed to analyze the LM in dogs with B. Bronchiseptica Infection in comparison with healthy dogs, and to assess the correlation between 16S rDNA amplicon sequencing and culture and qPCR/ and to correlate 16S rDNA amplicon sequencing with culture and qPCR results. Methods used: Twenty dogs with B. Bronchiseptica Infection and 4 healthy age-matched dogs were retrospectively included. B. Bronchiseptica Infection was diagnosed based on either positive qPCR (n=19/19) and/or positive culture (n=11/17, including 1 dog with culture available only) in the bronchoalveolar lavage fluid (BALF). qPCR for Mycoplasma cynos were also available in 18 of the dogs with B. Bronchiseptica Infection and the 4 healthy dogs. qPCRs results were categorized into 6 classes based on the cycle threshold values. 16S rDNA sequences were obtained from naïve BALF after DNA extraction, PCR targeting the V1-V3 region of the 16S rDNA and sequencing. Sequences were mapped on the SILVA database with an OTU clustering distance of 0.03. Total bacterial load was calculated with a qPCR targeting the V2-V3 region of the 16S rDNA. Analysis of the LM were performed using MOTHUR v1.39 for the ecological data calculation including the β and α-diversity, the richness and the evenness. Mann-Whitney tests were used to compare the ecological data and the bacterial load. Welsh t-tests and FDR correction were used to compare the differences in relative abundances. Correlations between sequencing results at each taxonomic level and qPCRs or culture were done by Spearman tests. Summary of the results: 16S rDNA sequencing results showed the presence of B. Bronchiseptica in large amounts in all BALF samples in diseased dogs. Seven of them were co-infected with M. cynos, one dog with another species of Mycoplasma, and one with bacteria of the Pseudomonas genus. A shift in the β-diversity of the LM was observed in diseased compared with healthy dogs (P=0.002). Compared with healthy dogs, there were significantly more Burkholderiaceae at family level and Bordetella at genus level (75.92 ± 25.99 versus 2.94 ± 2.56%; P=0.058 and 75.65 ± 26.04 versus 0.11 ± 0.19%; P=0.10 respectively) in diseased dogs. The richness and the α-diversity were significantly lower (93.09 ± 76.19 versus 340.50 ± 158.68; P=0.012 and 1.54 ± 0.48 versus 17.74 ± 9.36; P=0.006 respectively) and the bacterial load higher (5.72 ± 0.38 versus 4.93 ± 0.15; P=0.004) in diseased compared with healthy dogs. B. Bronchiseptica qPCR classes and positive culture results positively correlated with the relative abundance of B. Bronchiseptica obtained by 16S rDNA amplicon sequencing (r= 0.56, P=0.028 and r=0.70, P=0.002 respectively). M. cynos qPCR classes also positively correlated with the relative abundance of M. cynos (r=0.84, P

  • ANALYSIS OF THE LUNG MICROBIOTA IN DOGS WITH Bordetella Bronchiseptica Infection AND CORRELATION WITH CULTURE AND QUANTITATIVE POLYMERASE CHAIN REACTION
    2019
    Co-Authors: Fastrès Aline, Canonne-guibert Morgane, Taminiau Bernard, Billen Frédéric, Garigliany Mutien-marie, Daube Georges, Clercx Cécile
    Abstract:

    audience: researcher, professional, studentPurpose of the study: Infection with Bordetella Bronchiseptica, one of the principal pathogens involved in canine infectious respiratory disease complex (CIRD-C), can be diagnosed using bacterial culture or quantitative polymerase chain reaction (qPCR). The lung microbiota (LM) has been described in healthy experimental dogs, while it has not yet been studied in dogs with lower respiratory Infection. In the present study we aimed to analyze the LM in dogs with B. Bronchiseptica Infection in comparison with healthy dogs, and to assess the correlation between 16S rDNA amplicon sequencing and culture and qPCR/ and to correlate 16S rDNA amplicon sequencing with culture and qPCR results. Methods used: Twenty dogs with B. Bronchiseptica Infection and 4 healthy age-matched dogs were retrospectively included. B. Bronchiseptica Infection was diagnosed based on either positive qPCR (n=19/19) and/or positive culture (n=11/17, including 1 dog with culture available only) in the bronchoalveolar lavage fluid (BALF). qPCR for Mycoplasma cynos were also available in 18 of the dogs with B. Bronchiseptica Infection and the 4 healthy dogs. qPCRs results were categorized into 6 classes based on the cycle threshold values. 16S rDNA sequences were obtained from naïve BALF after DNA extraction, PCR targeting the V1-V3 region of the 16S rDNA and sequencing. Sequences were mapped on the SILVA database with an OTU clustering distance of 0.03. Total bacterial load was calculated with a qPCR targeting the V2-V3 region of the 16S rDNA. Analysis of the LM were performed using MOTHUR v1.39 for the ecological data calculation including the β and α-diversity, the richness and the evenness. Mann-Whitney tests were used to compare the ecological data and the bacterial load. Welsh t-tests and FDR correction were used to compare the differences in relative abundances. Correlations between sequencing results at each taxonomic level and qPCRs or culture were done by Spearman tests. Summary of the results: 16S rDNA sequencing results showed the presence of B. Bronchiseptica in large amounts in all BALF samples in diseased dogs. Seven of them were co-infected with M. cynos, one dog with another species of Mycoplasma, and one with bacteria of the Pseudomonas genus. A shift in the β-diversity of the LM was observed in diseased compared with healthy dogs (P=0.002). Compared with healthy dogs, there were significantly more Burkholderiaceae at family level and Bordetella at genus level (75.92 ± 25.99 versus 2.94 ± 2.56%; P=0.058 and 75.65 ± 26.04 versus 0.11 ± 0.19%; P=0.10 respectively) in diseased dogs. The richness and the α-diversity were significantly lower (93.09 ± 76.19 versus 340.50 ± 158.68; P=0.012 and 1.54 ± 0.48 versus 17.74 ± 9.36; P=0.006 respectively) and the bacterial load higher (5.72 ± 0.38 versus 4.93 ± 0.15; P=0.004) in diseased compared with healthy dogs. B. Bronchiseptica qPCR classes and positive culture results positively correlated with the relative abundance of B. Bronchiseptica obtained by 16S rDNA amplicon sequencing (r= 0.56, P=0.028 and r=0.70, P=0.002 respectively). M. cynos qPCR classes also positively correlated with the relative abundance of M. cynos (r=0.84, P