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Pierre Metzger - One of the best experts on this subject based on the ideXlab platform.
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Braunicetals: acetals from condensation of macrocyclic aldehydes and terpene diols in Botryococcus braunii.
Phytochemistry, 2008Co-Authors: Pierre Metzger, Marie Noelle Rager, Céline FosseAbstract:Two series of braunicetals were isolated from the green microalga Botryococcus braunii. Based on spectroscopic and chemical evidence, their structures were determined to be acetals formed by the condensation of C32 and C34 macrocyclic aldehydes with C33 and C34 methylated squalene diols (series I), or a C40 lycopaene diol (series II).
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Lycopanerols I-L, four new tetraterpenoid ethers from Botryococcus braunii.
Journal of Natural Products, 2003Co-Authors: Pierre Metzger, Marie Noelle Rager, Nicole Sellier, Claude LargeauAbstract:Four new tetraterpenoid ethers, lycopanerols I-L (5-8), were isolated from a culture of a strain of the green microalga Botryococcus braunii (L race). The structures were determined by means of spectral analyses and chemical degradation. The relative stereochemistry of these ethers was established by ROESY NMR. A biogenetic relationship is proposed between lycopanerols and lycopadiene (1), the acyclic diunsaturated tetraterpene hydrocarbon synthesized by the alga.
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lycopanerols h two high molecular weight ether lipids from Botryococcus braunii comprising an α tocopherol unit
Tetrahedron Letters, 2002Co-Authors: Pierre Metzger, Marie Noelle RagerAbstract:Two high molecular weight ether lipids, C151 and C153 lycopanerols H, have been isolated from the lipid extract of a strain of the green microalga Botryococcus braunii. These new compounds arise from the linkage via ether bridges of tetraterpenoid, n-alkylphenol and α-tocopherol units. Their structures have been elucidated by Mass and NMR spectral analysis.
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Botryolins A and B, two tetramethylsqualene triethers from the green microalga Botryococcus braunii
Phytochemistry, 2002Co-Authors: Pierre Metzger, Marie Noelle Rager, Claude LargeauAbstract:Two new triterpenoid polyethers with a tetramethylsqualene carbon skeleton, botryolins A and B, have been isolated from the green microalga Botryococcus braunii. Their structures were determined by means of spectral analyses including 2D NMR.
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six novel tetraterpenoid ethers lycopanerols b g and some other constituents from the green microalga Botryococcus braunii
Phytochemistry, 2000Co-Authors: Marie Noelle Rager, Pierre MetzgerAbstract:Abstract Six novel tetraterpenoid ethers, lycopanerols B-G, were isolated from lipidic extracts of the green microalga Botryococcus braunii (L race), along with a series of phytyl esters and α- and β-tocopherols. The structures of the compounds were determined by means of spectral analyses including 2D NMR techniques. A biogenetic relationship is proposed between lycopanerols and lycopadiene, the acyclic diunsaturated tetraterpenoid hydrocarbon synthesized by the alga.
Marie Noelle Rager - One of the best experts on this subject based on the ideXlab platform.
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Braunicetals: acetals from condensation of macrocyclic aldehydes and terpene diols in Botryococcus braunii.
Phytochemistry, 2008Co-Authors: Pierre Metzger, Marie Noelle Rager, Céline FosseAbstract:Two series of braunicetals were isolated from the green microalga Botryococcus braunii. Based on spectroscopic and chemical evidence, their structures were determined to be acetals formed by the condensation of C32 and C34 macrocyclic aldehydes with C33 and C34 methylated squalene diols (series I), or a C40 lycopaene diol (series II).
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Lycopanerols I-L, four new tetraterpenoid ethers from Botryococcus braunii.
Journal of Natural Products, 2003Co-Authors: Pierre Metzger, Marie Noelle Rager, Nicole Sellier, Claude LargeauAbstract:Four new tetraterpenoid ethers, lycopanerols I-L (5-8), were isolated from a culture of a strain of the green microalga Botryococcus braunii (L race). The structures were determined by means of spectral analyses and chemical degradation. The relative stereochemistry of these ethers was established by ROESY NMR. A biogenetic relationship is proposed between lycopanerols and lycopadiene (1), the acyclic diunsaturated tetraterpene hydrocarbon synthesized by the alga.
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lycopanerols h two high molecular weight ether lipids from Botryococcus braunii comprising an α tocopherol unit
Tetrahedron Letters, 2002Co-Authors: Pierre Metzger, Marie Noelle RagerAbstract:Two high molecular weight ether lipids, C151 and C153 lycopanerols H, have been isolated from the lipid extract of a strain of the green microalga Botryococcus braunii. These new compounds arise from the linkage via ether bridges of tetraterpenoid, n-alkylphenol and α-tocopherol units. Their structures have been elucidated by Mass and NMR spectral analysis.
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Botryolins A and B, two tetramethylsqualene triethers from the green microalga Botryococcus braunii
Phytochemistry, 2002Co-Authors: Pierre Metzger, Marie Noelle Rager, Claude LargeauAbstract:Two new triterpenoid polyethers with a tetramethylsqualene carbon skeleton, botryolins A and B, have been isolated from the green microalga Botryococcus braunii. Their structures were determined by means of spectral analyses including 2D NMR.
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six novel tetraterpenoid ethers lycopanerols b g and some other constituents from the green microalga Botryococcus braunii
Phytochemistry, 2000Co-Authors: Marie Noelle Rager, Pierre MetzgerAbstract:Abstract Six novel tetraterpenoid ethers, lycopanerols B-G, were isolated from lipidic extracts of the green microalga Botryococcus braunii (L race), along with a series of phytyl esters and α- and β-tocopherols. The structures of the compounds were determined by means of spectral analyses including 2D NMR techniques. A biogenetic relationship is proposed between lycopanerols and lycopadiene, the acyclic diunsaturated tetraterpenoid hydrocarbon synthesized by the alga.
Makoto M Watanabe - One of the best experts on this subject based on the ideXlab platform.
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A novel alphaproteobacterial ectosymbiont promotes the growth of the hydrocarbon-rich green alga Botryococcus braunii
Scientific Reports, 2015Co-Authors: Yuuhiko Tanabe, Masaki Yoshida, Yusuke Okazaki, Takashi Shiratori, Ken-ichiro Ishida, Hiroshi Matsuura, Shin-ichi Nakano, Makoto M WatanabeAbstract:Botryococcus braunii is a colony-forming green alga that accumulates large amounts of liquid hydrocarbons within the colony. The utilization of B. braunii for biofuel production is however hindered by its low biomass productivity. Here we describe a novel bacterial ectosymbiont (BOTRYCO-2) that confers higher biomass productivity to B. braunii . 16S rDNA analysis indicated that the sequence of BOTRYCO-2 shows low similarity (
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effects of soybean curd wastewater on the growth and hydrocarbon production of Botryococcus braunii strain bot 22
Bioresource Technology, 2012Co-Authors: Natsuki Yonezawa, Hiroshi Matsuura, Kunimitsu Kaya, Makoto Shiho, Makoto M WatanabeAbstract:Abstract A laboratory study was conducted to evaluate the possibility of using wastewater from a soybean curd manufacturing plant as a growth promoter of Botryococcus braunii strain BOT-22. Soybean curd wastewater (SCW) were added to AF-6 medium to set the final concentration to 0% (control), 1%, 2%, 5%, and 10% (v/v). The growth and hydrocarbon production observed in the cultures with 1% and 2% SCW were significantly higher than that observed in the control. It was postulated that proteins and/or reducing sugars in SCW could enhance the growth. A major finding was a shift in the chemical composition of hydrocarbons from C34H58 to C32H54 in association with increased concentrations of SCW. Considering the inorganic ions in SCW, it was presumed that a mixture of nitrate, 1–2% SCW, and secondarily treated SCW can be applied for mass cultivation of Botryococcus.
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Relationship between hydrocarbons and molecular phylogeny of Botryococcus braunii
Algal Research, 2012Co-Authors: Masanobu Kawachi, Takako Tanoi, Mikihide Demura, Kunimitsu Kaya, Makoto M WatanabeAbstract:Abstract The relationship between hydrocarbons and the molecular phylogeny of Botryococcus braunii was investigated using 31 axenic strains isolated in Japan. The resulting 18S rRNA tree, which includes additional sequences from the gene bank, revealed the existence of three major pylogenetic clades (I, II, and III) and four subclades (II1, II2, III1, and III2) with high bootstrap values. By gas chromatography/mass spectrometry analysis, nine hydrocarbon species were detected and categorized as 4 types: the three known races A, B, and L and the tentatively named race S comprising epoxy-n-alkane and saturated n-alkane chains with carbon numbers 18 and 20, respectively. Strains of clade I were coincident with race A. Clade II contained race B, and clade III contained race L and its derivatives, and race S. Clade III was further categorized into subclade III1 containing the typical race L strains and subclade III2 containing race S with high bootstrap values. The phylogenetic relationship of Botryococcus strains appeared to be in considerable agreement with unique hydrocarbon synthesis pathways. Maximum divergence values of 18S rRNA indicated large speciation among the clades at almost the species level, suggesting the utility of biochemical properties, e.g., hydrocarbon types, in determining the taxonomy of Botryococcus .
Lei Fang - One of the best experts on this subject based on the ideXlab platform.
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transcriptomic analysis of a moderately growing subisolate Botryococcus braunii 779 chlorophyta in response to nitrogen deprivation
Biotechnology for Biofuels, 2015Co-Authors: Lei Fang, Deying Sun, Xin Chen, Wee Chew, Jianhua LiuAbstract:Background The colonial microalga Botryococcus braunii has been brought to people’s attention for its conspicuous ability to accumulate a variety of lipids including hydrocarbons. B. braunii strains are classified into 3 races based on the types of hydrocarbons. A and B races are known to accumulate high level of lipids. However, their extreme slow growth rate has impeded its application for renewable biofuel production.
Paran Gani - One of the best experts on this subject based on the ideXlab platform.
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Extraction of hydrocarbons from freshwater green microalgae (Botryococcus sp.) biomass after phycoremediation of domestic wastewater.
International Journal of Phytoremediation, 2017Co-Authors: Paran Gani, Norshuhaila Mohamed Sunar, Hazel Monica Matias-peralta, Aziz Abdul Latiff, Radin Maya Saphira Radin Mohamed, U.k. ParjoAbstract:ABSTRACTThis study was undertaken to analyze the efficiency of Botryococcus sp. in the phycoremediation of domestic wastewater and to determine the variety of hydrocarbons derived from microalgal oil after phycoremediation. The study showed a significant (p < 0.05) reduction of pollutant loads of up to 93.9% chemical oxygen demand, 69.1% biochemical oxygen demand, 59.9% total nitrogen, 54.5% total organic carbon, and 36.8% phosphate. The average dry weight biomass produce was 0.1 g/L of wastewater. In addition, the dry weight biomass of Botryococcus sp. was found to contain 72.5% of crude oil. The composition analysis using Gas Chromatogram - Mass Spectrometry (GC-MS) found that phthalic acid, 2-ethylhexyltridecyl ester (C29H48O4), contributed the highest percentage (71.6%) of the total hydrocarbon compounds to the extracted algae oil. The result of the study suggests that Botryococcus sp. can be used for effective phycoremediation, as well as to provide a sustainable hydrocarbon source as a value-added c...
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A Verhulst model for microalgae Botryococcus sp. growth and nutrient removal in wastewater
2017Co-Authors: Siti Suhana Jamaian, Noorhadila Mohd Bakeri, Norshuhaila Mohamed Sunar, Paran GaniAbstract:Microalgae Botryococcus sp. is a colonial green alga found in lakes and reservoirs in Malaysia. Previous studies reported that the potential of Botryococcus sp. photosynthesis as a source of fuel. The Botryococcus sp. contains hydrocarbon up to 75% of dry weight, which can be converted into petrol, diesel or turbine fuel or other liquid or gaseous hydrocarbons. Recently, an experimental study was conducted on phycoremediation technology for wastewater using Botryococcus sp. The phycoremediation technology is useful to remove the excess of nutrients such as nitrogen, phosphorus and also have the ability to remove various pollutants from wastewater. This research implements the Verhulst model to estimate the nutrient removal by microalgae Botryococcus sp. from the wastewater. This model has been validated with the experiments of microalgae Botryococcus sp. grown in domestic and palm oil wastewater. The results suggested that microalgae Botryococcus sp. could be cultured in domestic and palm oil wastewater w...
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Phycoremediation using Botryococcus sp. as nutrients removal in organic wastewaters coupled with hydrocarbon production
2017Co-Authors: Paran GaniAbstract:Rapid population growth and industrial development are expected to contribute extremely to the world environmental crisis due to the excessive wastewater generation, global warming, climate change and increased use of petroleum fuels. In response to the problems, new technology via phycoremediation to reduce the wastewater contamination coupled with production of sustainable hydrocarbon has received much interest worldwide. Thus, the aim of the study is to produce the hydrocarbon from microalgae, Botryococcus sp. combined with phycoremediation of domestic wastewater (DW) and food processing wastewater (FW). The Botryococcus sp. locally isolated from the tropical rainforest. The optimisation study proved that the Botryococcus sp. grew well in the temperature of 23-33°C, the light intensity of 243 μmol m-2s-1 and 24 hours of light exposure. In fact, this Botryococcus sp. much more tolerated with the outdoor condition when integrated with wastewater phycoremediation in term of biomass productivity and wastewaters bioremediation. The best microalgae concentration was performed at 106 cells/mL for both wastewaters. The highest removal of nutrients (TP, TN and TOC) in DW and FW up to 100% and 92.8%, respectively under outdoor condition; while 95.4% and 76.4%, respectively under indoor condition. Selected heavy metal (Zn, Fe, Cd, Mn) study showed a very significant reduction (p
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Application of phycoremediation technology in the treatment of food processing wastewater by freshwater microalgae Botryococcus sp.
2016Co-Authors: Paran Gani, Norshuhaila Mohamed Sunar, Hazel Monica Matias-peralta, Aziz Abdul LatiffAbstract:The cultivating of freshwater microalgae namely Botryococcus sp. in outdoor conditions for phycoremediation of food processing wastewater was investigated. Dilution of wastewater and distilled water as pure sample (100%) and diluted sample (50%) of wastewater were examined. The highest growth of Botryococcus sp. in pure sample of food processing wastewater occurs at Day 12 at approximately 3.72×106 cell/mL with the highest removal of nitrate, phosphate, and total organic carbon are 86.62%, 78.23% and 76.66%, respectively. Diluted (50%) food processing wastewater, the Botryococcus sp. was able to reach maximum growth at Day 13 at cell concentration approximately at 9.7×105 cell/mL. Significantly reduction for some nutrients such as nitrate (78.78%), biochemical oxygen demand (69.03%) and total organic carbon (67.93%) were observed. This finding proved good indication of Botryococcus sp. grows in food processing wastewater. The information from these findings is potentially useful for biotechnology industries for further development of bio-based product from microalgae biomass.
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Experimental Study for Phycoremediation of Botryococcus Sp. on Greywater
Applied Mechanics and Materials, 2015Co-Authors: Paran Gani, Norshuhaila Mohamed Sunar, Hazel Monica Matias-peralta, Aziz Abdul Latiff, Nabila Syazwanie Kamaludin, U.k. Parjo, Q. EmparanAbstract:Greywater (GW) is identified as waste disposal from home activites that is discharging from laundry, bath and wash-basin. GW useful in irrigation of a garden and aids to reduce cost as well as maintain the environmental prosperity. This paper discussed the effectiveness of Botryococcus sp. to clean GW in phycoremediation treatment. This process involves as growing the Botryococcus sp. in the GW which is contributing to utilize supplements in GW for its grow. The results indicated that Botryococcus sp. is effective to reduce COD (88%), BOD (82%), TIC (76%), TC (58%), TN (52%), TOC (39%), Phosphate (37.5%) and pH (7%) for 100% concentration of GW. Meanwhile, for the 50% of GW concentration Botryococcus sp. capable to remove such as COD (83%), TIC (82%), BOD (68%), TN (67%), Phosphate (36.8%), TC (34%), TOC (31%) and pH (1.2%). Then, the study concludes that Botryococcus sp. can grow effectively in GW and be able to reduce the rate of nutrient in GW.