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V. Yaylayan - One of the best experts on this subject based on the ideXlab platform.
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Volatile metabolite profiling for the discrimination of onion bulbs infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii
European Journal of Plant Pathology, 2004Co-Authors: Balakrishnan Prithiviraj, Ajit Vikram, Ajjamada C Kushalappa, V. YaylayanAbstract:The volatile metabolites of the headspace gas of onion bulbs inoculated with three different pathogens, Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii, were profiled using gas chromatography/mass spectrometry. Differences in the number and amount of volatile metabolites were observed. Two hundred and fifty three volatile metabolites were detected in bulbs inoculated with three pathogens or sterile distilled water. On day three, 202 volatile metabolites were observed, compared to 166 on day six. Of the 253 compounds, however, only 59 occurred relatively consistently over replications, of which 25 compounds were specific to one or more pathogens, including 10 that were unique to a pathogen. Metabolites such as 1-Oxa-4,6-diazacyclooctane-5-thione and 4-mercapto-3-(methylthio)-ς-(thio-lactone)-crotonic acid were exclusive to onions inoculated with F. oxysporum. Acetone, acetic acid-hydrazide, propylcarbamate, 1-bromo-1-propene, thiirane, 1-(methylthio)-E-1-propene and 1-ethenyl-4-ethyl-benzene were specific to B. Allii. 3-bromo-furan was specific to E. carotovora ssp. carotovora. Sterile water-inoculated bulbs produced 3,3′-dioxy-1,2-propanediol-tetranitrate. Highest amount of sulfurs was found in pathogen-inoculated, while highest amounts of terpenes, aromatics and aliphatics were found in sterile distilled water-inoculated bulbs. The possible use of these differences in the volatile metabolites for detecting and discriminating diseases of onion in storage is discussed.
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Volatile Metabolite Profiling for the Discrimination of Onion Bulbs Infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii
European Journal of Plant Pathology, 2004Co-Authors: Balakrishnan Prithiviraj, Ajit Vikram, Ajjamada C Kushalappa, V. YaylayanAbstract:The volatile metabolites of the headspace gas of onion bulbs inoculated with three different pathogens, Erwinia carotovora ssp. carotovora , Fusarium oxysporum and Botrytis Allii , were profiled using gas chromatography/mass spectrometry. Differences in the number and amount of volatile metabolites were observed. Two hundred and fifty three volatile metabolites were detected in bulbs inoculated with three pathogens or sterile distilled water. On day three, 202 volatile metabolites were observed, compared to 166 on day six. Of the 253 compounds, however, only 59 occurred relatively consistently over replications, of which 25 compounds were specific to one or more pathogens, including 10 that were unique to a pathogen. Metabolites such as 1-Oxa-4,6-diazacyclooctane-5-thione and 4-mercapto-3-(methylthio)-ς-(thio-lactone)-crotonic acid were exclusive to onions inoculated with F. oxysporum . Acetone, acetic acid-hydrazide, propylcarbamate, 1-bromo-1-propene, thiirane, 1-(methylthio)- E -1-propene and 1-ethenyl-4-ethyl-benzene were specific to B. Allii . 3-bromo-furan was specific to E. carotovora ssp. carotovora . Sterile water-inoculated bulbs produced 3,3′-dioxy-1,2-propanediol-tetranitrate. Highest amount of sulfurs was found in pathogen-inoculated, while highest amounts of terpenes, aromatics and aliphatics were found in sterile distilled water-inoculated bulbs. The possible use of these differences in the volatile metabolites for detecting and discriminating diseases of onion in storage is discussed.
Ajjamada C Kushalappa - One of the best experts on this subject based on the ideXlab platform.
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volatile metabolites from the headspace of onion bulbs inoculated with postharvest pathogens as a tool for disease discrimination
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie, 2005Co-Authors: Ajit Vikram, Habiballah Hamzehzarghani, Ajjamada C KushalappaAbstract:Gas chromatography and mass spectrometry was used to analyze volatiles in the headspace of 'Fortress' onion bulbs inoculated with Fusarium oxysporum, Botrytis Allii, Erwinia carotovora subsp. carotovora, Aspergillus niger, or Penicillium aurantiogriseum. A total of 130 volatile metabolites were detected, of which 28 occurred more than four times in seven replicates and two incubation periods. Out of 28 relatively consistent compounds, 12 compounds were specific to one or more diseases or inoculations. Ethyl cyclobutane was specific to bulbs inoculated with F. oxysporum, while styrene was common to A. niger, E. carotovora subsp. carotovora, and F. oxysporum, with the highest abundance in the latter. N-3-Aminophenyl acetamide was common to E. carotovora subsp. carotovora, P. aurantiogriseum, and the water inoculated control. 2-Azabicyclo[3.2.0]hept-6-ene was detected in bulbs inoculated with A. niger, E. carotovora subsp. carotovora, and P. aurantiogriseum. Discriminant analysis models based on metabolic fi...
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Volatile metabolite profiling for the discrimination of onion bulbs infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii
European Journal of Plant Pathology, 2004Co-Authors: Balakrishnan Prithiviraj, Ajit Vikram, Ajjamada C Kushalappa, V. YaylayanAbstract:The volatile metabolites of the headspace gas of onion bulbs inoculated with three different pathogens, Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii, were profiled using gas chromatography/mass spectrometry. Differences in the number and amount of volatile metabolites were observed. Two hundred and fifty three volatile metabolites were detected in bulbs inoculated with three pathogens or sterile distilled water. On day three, 202 volatile metabolites were observed, compared to 166 on day six. Of the 253 compounds, however, only 59 occurred relatively consistently over replications, of which 25 compounds were specific to one or more pathogens, including 10 that were unique to a pathogen. Metabolites such as 1-Oxa-4,6-diazacyclooctane-5-thione and 4-mercapto-3-(methylthio)-ς-(thio-lactone)-crotonic acid were exclusive to onions inoculated with F. oxysporum. Acetone, acetic acid-hydrazide, propylcarbamate, 1-bromo-1-propene, thiirane, 1-(methylthio)-E-1-propene and 1-ethenyl-4-ethyl-benzene were specific to B. Allii. 3-bromo-furan was specific to E. carotovora ssp. carotovora. Sterile water-inoculated bulbs produced 3,3′-dioxy-1,2-propanediol-tetranitrate. Highest amount of sulfurs was found in pathogen-inoculated, while highest amounts of terpenes, aromatics and aliphatics were found in sterile distilled water-inoculated bulbs. The possible use of these differences in the volatile metabolites for detecting and discriminating diseases of onion in storage is discussed.
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Volatile Metabolite Profiling for the Discrimination of Onion Bulbs Infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii
European Journal of Plant Pathology, 2004Co-Authors: Balakrishnan Prithiviraj, Ajit Vikram, Ajjamada C Kushalappa, V. YaylayanAbstract:The volatile metabolites of the headspace gas of onion bulbs inoculated with three different pathogens, Erwinia carotovora ssp. carotovora , Fusarium oxysporum and Botrytis Allii , were profiled using gas chromatography/mass spectrometry. Differences in the number and amount of volatile metabolites were observed. Two hundred and fifty three volatile metabolites were detected in bulbs inoculated with three pathogens or sterile distilled water. On day three, 202 volatile metabolites were observed, compared to 166 on day six. Of the 253 compounds, however, only 59 occurred relatively consistently over replications, of which 25 compounds were specific to one or more pathogens, including 10 that were unique to a pathogen. Metabolites such as 1-Oxa-4,6-diazacyclooctane-5-thione and 4-mercapto-3-(methylthio)-ς-(thio-lactone)-crotonic acid were exclusive to onions inoculated with F. oxysporum . Acetone, acetic acid-hydrazide, propylcarbamate, 1-bromo-1-propene, thiirane, 1-(methylthio)- E -1-propene and 1-ethenyl-4-ethyl-benzene were specific to B. Allii . 3-bromo-furan was specific to E. carotovora ssp. carotovora . Sterile water-inoculated bulbs produced 3,3′-dioxy-1,2-propanediol-tetranitrate. Highest amount of sulfurs was found in pathogen-inoculated, while highest amounts of terpenes, aromatics and aliphatics were found in sterile distilled water-inoculated bulbs. The possible use of these differences in the volatile metabolites for detecting and discriminating diseases of onion in storage is discussed.
Ajit Vikram - One of the best experts on this subject based on the ideXlab platform.
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volatile metabolites from the headspace of onion bulbs inoculated with postharvest pathogens as a tool for disease discrimination
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie, 2005Co-Authors: Ajit Vikram, Habiballah Hamzehzarghani, Ajjamada C KushalappaAbstract:Gas chromatography and mass spectrometry was used to analyze volatiles in the headspace of 'Fortress' onion bulbs inoculated with Fusarium oxysporum, Botrytis Allii, Erwinia carotovora subsp. carotovora, Aspergillus niger, or Penicillium aurantiogriseum. A total of 130 volatile metabolites were detected, of which 28 occurred more than four times in seven replicates and two incubation periods. Out of 28 relatively consistent compounds, 12 compounds were specific to one or more diseases or inoculations. Ethyl cyclobutane was specific to bulbs inoculated with F. oxysporum, while styrene was common to A. niger, E. carotovora subsp. carotovora, and F. oxysporum, with the highest abundance in the latter. N-3-Aminophenyl acetamide was common to E. carotovora subsp. carotovora, P. aurantiogriseum, and the water inoculated control. 2-Azabicyclo[3.2.0]hept-6-ene was detected in bulbs inoculated with A. niger, E. carotovora subsp. carotovora, and P. aurantiogriseum. Discriminant analysis models based on metabolic fi...
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Volatile metabolite profiling for the discrimination of onion bulbs infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii
European Journal of Plant Pathology, 2004Co-Authors: Balakrishnan Prithiviraj, Ajit Vikram, Ajjamada C Kushalappa, V. YaylayanAbstract:The volatile metabolites of the headspace gas of onion bulbs inoculated with three different pathogens, Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii, were profiled using gas chromatography/mass spectrometry. Differences in the number and amount of volatile metabolites were observed. Two hundred and fifty three volatile metabolites were detected in bulbs inoculated with three pathogens or sterile distilled water. On day three, 202 volatile metabolites were observed, compared to 166 on day six. Of the 253 compounds, however, only 59 occurred relatively consistently over replications, of which 25 compounds were specific to one or more pathogens, including 10 that were unique to a pathogen. Metabolites such as 1-Oxa-4,6-diazacyclooctane-5-thione and 4-mercapto-3-(methylthio)-ς-(thio-lactone)-crotonic acid were exclusive to onions inoculated with F. oxysporum. Acetone, acetic acid-hydrazide, propylcarbamate, 1-bromo-1-propene, thiirane, 1-(methylthio)-E-1-propene and 1-ethenyl-4-ethyl-benzene were specific to B. Allii. 3-bromo-furan was specific to E. carotovora ssp. carotovora. Sterile water-inoculated bulbs produced 3,3′-dioxy-1,2-propanediol-tetranitrate. Highest amount of sulfurs was found in pathogen-inoculated, while highest amounts of terpenes, aromatics and aliphatics were found in sterile distilled water-inoculated bulbs. The possible use of these differences in the volatile metabolites for detecting and discriminating diseases of onion in storage is discussed.
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Volatile Metabolite Profiling for the Discrimination of Onion Bulbs Infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii
European Journal of Plant Pathology, 2004Co-Authors: Balakrishnan Prithiviraj, Ajit Vikram, Ajjamada C Kushalappa, V. YaylayanAbstract:The volatile metabolites of the headspace gas of onion bulbs inoculated with three different pathogens, Erwinia carotovora ssp. carotovora , Fusarium oxysporum and Botrytis Allii , were profiled using gas chromatography/mass spectrometry. Differences in the number and amount of volatile metabolites were observed. Two hundred and fifty three volatile metabolites were detected in bulbs inoculated with three pathogens or sterile distilled water. On day three, 202 volatile metabolites were observed, compared to 166 on day six. Of the 253 compounds, however, only 59 occurred relatively consistently over replications, of which 25 compounds were specific to one or more pathogens, including 10 that were unique to a pathogen. Metabolites such as 1-Oxa-4,6-diazacyclooctane-5-thione and 4-mercapto-3-(methylthio)-ς-(thio-lactone)-crotonic acid were exclusive to onions inoculated with F. oxysporum . Acetone, acetic acid-hydrazide, propylcarbamate, 1-bromo-1-propene, thiirane, 1-(methylthio)- E -1-propene and 1-ethenyl-4-ethyl-benzene were specific to B. Allii . 3-bromo-furan was specific to E. carotovora ssp. carotovora . Sterile water-inoculated bulbs produced 3,3′-dioxy-1,2-propanediol-tetranitrate. Highest amount of sulfurs was found in pathogen-inoculated, while highest amounts of terpenes, aromatics and aliphatics were found in sterile distilled water-inoculated bulbs. The possible use of these differences in the volatile metabolites for detecting and discriminating diseases of onion in storage is discussed.
Balakrishnan Prithiviraj - One of the best experts on this subject based on the ideXlab platform.
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Volatile metabolite profiling for the discrimination of onion bulbs infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii
European Journal of Plant Pathology, 2004Co-Authors: Balakrishnan Prithiviraj, Ajit Vikram, Ajjamada C Kushalappa, V. YaylayanAbstract:The volatile metabolites of the headspace gas of onion bulbs inoculated with three different pathogens, Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii, were profiled using gas chromatography/mass spectrometry. Differences in the number and amount of volatile metabolites were observed. Two hundred and fifty three volatile metabolites were detected in bulbs inoculated with three pathogens or sterile distilled water. On day three, 202 volatile metabolites were observed, compared to 166 on day six. Of the 253 compounds, however, only 59 occurred relatively consistently over replications, of which 25 compounds were specific to one or more pathogens, including 10 that were unique to a pathogen. Metabolites such as 1-Oxa-4,6-diazacyclooctane-5-thione and 4-mercapto-3-(methylthio)-ς-(thio-lactone)-crotonic acid were exclusive to onions inoculated with F. oxysporum. Acetone, acetic acid-hydrazide, propylcarbamate, 1-bromo-1-propene, thiirane, 1-(methylthio)-E-1-propene and 1-ethenyl-4-ethyl-benzene were specific to B. Allii. 3-bromo-furan was specific to E. carotovora ssp. carotovora. Sterile water-inoculated bulbs produced 3,3′-dioxy-1,2-propanediol-tetranitrate. Highest amount of sulfurs was found in pathogen-inoculated, while highest amounts of terpenes, aromatics and aliphatics were found in sterile distilled water-inoculated bulbs. The possible use of these differences in the volatile metabolites for detecting and discriminating diseases of onion in storage is discussed.
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Volatile Metabolite Profiling for the Discrimination of Onion Bulbs Infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis Allii
European Journal of Plant Pathology, 2004Co-Authors: Balakrishnan Prithiviraj, Ajit Vikram, Ajjamada C Kushalappa, V. YaylayanAbstract:The volatile metabolites of the headspace gas of onion bulbs inoculated with three different pathogens, Erwinia carotovora ssp. carotovora , Fusarium oxysporum and Botrytis Allii , were profiled using gas chromatography/mass spectrometry. Differences in the number and amount of volatile metabolites were observed. Two hundred and fifty three volatile metabolites were detected in bulbs inoculated with three pathogens or sterile distilled water. On day three, 202 volatile metabolites were observed, compared to 166 on day six. Of the 253 compounds, however, only 59 occurred relatively consistently over replications, of which 25 compounds were specific to one or more pathogens, including 10 that were unique to a pathogen. Metabolites such as 1-Oxa-4,6-diazacyclooctane-5-thione and 4-mercapto-3-(methylthio)-ς-(thio-lactone)-crotonic acid were exclusive to onions inoculated with F. oxysporum . Acetone, acetic acid-hydrazide, propylcarbamate, 1-bromo-1-propene, thiirane, 1-(methylthio)- E -1-propene and 1-ethenyl-4-ethyl-benzene were specific to B. Allii . 3-bromo-furan was specific to E. carotovora ssp. carotovora . Sterile water-inoculated bulbs produced 3,3′-dioxy-1,2-propanediol-tetranitrate. Highest amount of sulfurs was found in pathogen-inoculated, while highest amounts of terpenes, aromatics and aliphatics were found in sterile distilled water-inoculated bulbs. The possible use of these differences in the volatile metabolites for detecting and discriminating diseases of onion in storage is discussed.
C.l.m. De Visser - One of the best experts on this subject based on the ideXlab platform.
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Bestrijding van koprot in uien
2004Co-Authors: M.c. Plentinger, R.c.f.m. Van Den Broek, M. Huisman-de Lange, C.l.m. De VisserAbstract:Dit onderzoeksproject had de volgende doelstellingen: 1. vaststellen van het (neven-)effect van toegelaten fungiciden in uien op een infectie door Botrytis Allii, de veroorzaker van koprot in uien; 2. de vraag beantwoorden of er perspectieven zijn om de sporulatie dan wel de aanwezigheid van de schimmel te detecteren; 3. In 2003 proberen om m.b.v. een adviessysteem het tijdstip van bespuiten nauwkeuriger vast te stellen.
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Onderzoek naar effectieve chemische bestrijding van bladvlekkenziekte en koprot en naar voorspelling van koprot in uien = Research into effective chemical control of leaf spot and neck rot and into methods to predict neck rot in onions
1994Co-Authors: C.l.m. De Visser, L. Hoekstra, D. HoekAbstract:In de periode 1988-1993 zijn kas- en veldproeven met uien uitgevoerd waarin het effect is nagegaan van diverse fungiciden op bladvlekkenziekte en koprot In een aantal proeven is bovendien onderzocht of de werkingsduur van vier middelen verschilde door ze te vergelijken in een 7-daags en in een 14-daags spuitschema. Daarnaast is onderzoek verricht naar het verband tussen de aanwezigheid van Botrytis Allii in bladtoppen gedurende het groeiseizoen en het uiteindelijk percentage koprot in bewaring.