The Experts below are selected from a list of 3672 Experts worldwide ranked by ideXlab platform

Sante Roperto - One of the best experts on this subject based on the ideXlab platform.

  • Bovine Papillomavirus type 13 expression in the urothelial bladder tumours of cattle
    Transboundary and Emerging Diseases, 2016
    Co-Authors: Sante Roperto, Valeria Russo, Marita Georgia Riccardi, Leonardo Leonardi, Manuela Martano, Federica Corrado, F Roperto
    Abstract:

    Bovine Papillomavirus type 13 (BPV-13), a novel DeltaPapillomavirus, has been found associated with urothelial tumours of the urinary bladder of cattle grazing on lands infested with bracken fern. BPV-13 was detected in 28 of 39 urothelial tumours. Diagnosis was based on sequencing of L1 and E5 amplicons from tumour samples. The nucleotide sequences generated from these amplicons showed a 100% homology with the sequences of BPV-13 L1 and E5 DNA found in Brazil from a fibropapilloma of the ear in a cow and from equine sarcoids in two horses. GenBank accession number of our representative BPV-13 sequences is JQ798171.1. Furthermore, mRNA encoding BPV-13 E5 oncoprotein was also documented, and its expression was also shown by immunohistochemistry and immunofluorescence in the basal and suprabasal urothelial tumour cells. In twenty-three tumours, BPV-13 was simultaneously found with BPV-2, a DeltaPapillomavirus genus, species 4. The latter virus was detected by amplifying and sequencing a 154-bp-sized DNA fragment of BPV-2 E5. In addition, BPV-13 by itself was seen to be expressed in five BPV-2-negative urothelial tumours. This study shows that BPV-13 is present in urothelial tumour cells thus sharing biological properties with BPV-1 and BPV-2. Although further studies are needed, BPV-13 appears to be another worldwide infectious agent responsible for a distressing disease causing severe economic losses in cattle industry.

  • Bovine Papillomavirus type 2 bpv 2 e5 oncoprotein binds to the subunit d of the v1 atpase proton pump in naturally occurring urothelial tumors of the urinary bladder of cattle
    PLOS ONE, 2014
    Co-Authors: Sante Roperto, Valeria Russo, Iolanda Esposito, Marita Georgia Riccardi, Chiara Urraro, Roberta De Luca, Giuseppe Borzacchiello, Cinzia Raso, Marco Gaspari, Dora Maria Ceccarelli
    Abstract:

    Background Active infection by Bovine Papillomavirus type 2 (BPV-2) was documented for fifteen urinary bladder tumors in cattle. Two were diagnosed as papillary urothelial neoplasm of low malignant potential (PUNLMP), nine as papillary and four as invasive urothelial cancers.

  • productive infection of Bovine Papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes
    PLOS ONE, 2013
    Co-Authors: Sante Roperto, Valeria Russo, Ayhan Ozkul, Annunziata Corteggio, Aylin Sepicidincel, C Catoi, Iolanda Esposito, Marita Georgia Riccardi, Chiara Urraro, Roberta De Luca
    Abstract:

    Background Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of Bovine Papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring Papillomavirus-associated urothelial bladder tumors.

  • pbmcs are additional sites of productive infection of Bovine Papillomavirus type 2
    Journal of General Virology, 2011
    Co-Authors: Sante Roperto, Valeria Russo, Iolanda Esposito, Chiara Urraro, Roberta De Luca, Giuseppe Borzacchiello, S Comazzi, Emilio Ciusani, Francesca Paolini, Aldo Venuti
    Abstract:

    Bovine Papillomavirus type 2 (BPV-2) is an oncogenic virus infecting both epithelial and mesenchymal cells. Its life cycle, similar to other Papillomaviruses (PVs), appears to be linked to epithelial differentiation. Human and Bovine PVs have been known to reside in a latent, episomal form in PBMCs; therefore, it is believed that blood cells, like all mesenchymal cells, function as non-permissive carriers. Here, for the first time in veterinary and comparative medicine, the BPV-2 E5 oncoprotein and the major structural L1 capsid protein, known to be expressed only in productive infections, were shown to occur in defined subsets of PBMCs. E5 oncoprotein was detected in sorted T- and B-cells as well as in monocytes by flow cytometry and Western blot analysis. However, CD4+ and CD8+ lymphocytes appeared to be the main circulating targets of the virus, thus possibly representing the most important reservoir of active BPV-2 in blood. L1 protein was identified by flow cytometry in a population of blood cells recognized as lymphocytes by morphological scatter properties. Western blot analysis was performed on lysates obtained from the sorted subpopulations of PBMCs and detected L1 protein in CD4+ and CD8+ cells only. Thus, this study showed that CD4+ and CD8+ lymphocytes are permissive for BPV-2 and are new, hitherto unknown sites of productive PV infection. In light of these observations, the life cycle of PVs needs to be revisited to gain novel insights into the epidemiology of BPV infection and the pathogenesis of related diseases.

  • expression of platelet derived growth factor β receptor and Bovine Papillomavirus e5 and e7 oncoproteins in equine sarcoid
    Journal of Comparative Pathology, 2008
    Co-Authors: Giuseppe Borzacchiello, Sante Roperto, Valeria Russo, L Della Salda, F Roperto
    Abstract:

    Equine sarcoids are benign fibroblastic skin tumours that are recognized throughout the world. Infection with Bovine Papillomavirus (BPV) types 1 and 2 has been implicated as a major factor in disease development; however, the cellular mechanisms underlying fibroblast transformation remain poorly defined. The present study further characterizes aspects of the association with BPV in 15 equine sarcoids. BPV DNA was demonstrated in 12/15 tumours collected from different areas of Italy. Nine of these 12 tumours expressed the BPV oncoproteins E5 and E7, but these oncoproteins were not expressed by normal equine cells. The BPV E5 protein is known to bind to the platelet-derived growth factor-beta receptor (PDGF-betaR) and this molecule was expressed by 11 of the 12 sarcoids in which E5 was demonstrated. These findings add further weight to the theory that BPV and the PDGF-betaR may have a role in the pathogenesis of this disease.

Daniel Dimaio - One of the best experts on this subject based on the ideXlab platform.

  • in vitro dimerization of the Bovine Papillomavirus e5 protein transmembrane domain
    Biochemistry, 2008
    Co-Authors: Joanne Oates, Daniel Dimaio, Matthew R Hicks, Timothy R Dafforn, Ann M Dixon
    Abstract:

    The E5 protein from Bovine Papillomavirus is a type II membrane protein and the product of the smallest known oncogene. E5 causes cell transformation by binding and activating the platelet-derived growth factor beta receptor (PDGF beta R). In order to productively interact with the receptor, it is thought that E5 binds as a dimer. However, wild-type E5 and various mutants have also been shown to form trimers, tetramers, and even higher order oligomers. The residues in E5 that drive and stabilize a dimeric state are also still in question. At present, two different models for the E5 dimer exist in the literature, one symmetric and one asymmetric. There is universal agreement, however, that the transmembrane (TM) domain plays a vital role in stabilizing the functional oligomer; indeed, mutation of various TM domain residues can abolish E5 function. In order to better resolve the role of the E5 TM domain in function, we have undertaken the first quantitative in vitro characterization of the E5 TM domain in detergent micelles and liposomes. Circular and linear dichroism analyses verify that the TM domain adopts a stable alpha-helical structure and is able to partition efficiently across lipid bilayers. SDS-PAGE and analytical ultracentrifugation demonstrate for the first time that the TM domain of E5 forms a strong dimer with a standard state free energy of dissociation of 5.0 kcal mol(-1). We have used our new results to interpret existing models of E5 dimer formation and provide a direct link between TM helix interactions and E5 function.

  • productive interaction between transmembrane mutants of the Bovine Papillomavirus e5 protein and the platelet derived growth factor β receptor
    Journal of Virology, 2005
    Co-Authors: Charchang Lai, Anne P B Edwards, Daniel Dimaio
    Abstract:

    The Bovine Papillomavirus E5 protein is a 44-amino-acid transmembrane protein that transforms cells by binding to the transmembrane region of the cellular platelet-derived growth factor (PDGF) β receptor, resulting in sustained receptor signaling. However, there are published reports that certain mutants with amino acid substitutions in the membrane-spanning segment of the E5 protein transform cells without activating the PDGF β receptor. We re-examined several of these transmembrane mutants, and here we present five lines of evidence that these mutants do in fact activate the PDGF β receptor, resulting in cellular signaling and transformation.

  • Stable association between the Bovine Papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells
    Proceedings of the National Academy of Sciences of the United States of America, 1992
    Co-Authors: Lisa M. Petti, Daniel Dimaio
    Abstract:

    Abstract The 44-amino acid E5 transforming protein of Bovine Papillomavirus is the shortest protein known to induce tumorigenic transformation of fibroblasts. We showed previously that expression of the E5 protein activates the cellular beta receptor for platelet-derived growth factor (PDGF) and proposed that the activated receptor transmits the transforming signal to the cell. Here we use coimmunoprecipitation analysis to show that the E5 protein and the activated PDGF receptor exist in a stable complex in transformed mouse C127 cells. These results suggest a distinct mechanism of growth factor receptor activation and provide further evidence that the PDGF receptor is an important target of the E5 protein.

  • activation of the platelet derived growth factor receptor by the Bovine Papillomavirus e5 transforming protein
    The EMBO Journal, 1991
    Co-Authors: Lisa M. Petti, L A Nilson, Daniel Dimaio
    Abstract:

    The Bovine Papillomavirus E5 gene encodes a 44 amino acid membrane-associated protein that can induce tumorigenic transformation of rodent fibroblast cell lines. Genetic studies suggest that the E5 protein may transform cells by influencing the activity of cellular proteins involved in growth regulation. We report here that the endogenous cellular beta type receptor for the platelet-derived growth factor (PDGF) is constitutively activated in C127 and FR3T3 cells stably transformed by the E5 protein, but not in these cell types transformed by a variety of other oncogenes. In C127 cells, a metabolic precursor as well as the mature form of the receptor is activated by E5 transformation. Activation of the receptor also occurs upon acute E5-mediated transformation of these cells and precedes mitogenic stimulation in this system. Moreover, activation of the receptor by addition of PDGF or the v-sis gene to untransformed cells is sufficient to induce DNA synthesis and stable growth transformation. We propose that the PDGF receptor is an important cellular intermediate in the transforming activity of the Bovine Papillomavirus E5 protein. There is a short region of sequence similarity between the fibroPapillomavirus E5 proteins and PDGF, suggesting that the E5 proteins may activate the PDGF receptor by binding directly to it.

Giuseppe Borzacchiello - One of the best experts on this subject based on the ideXlab platform.

  • Bovine Papillomavirus type 2 bpv 2 e5 oncoprotein binds to the subunit d of the v1 atpase proton pump in naturally occurring urothelial tumors of the urinary bladder of cattle
    PLOS ONE, 2014
    Co-Authors: Sante Roperto, Valeria Russo, Iolanda Esposito, Marita Georgia Riccardi, Chiara Urraro, Roberta De Luca, Giuseppe Borzacchiello, Cinzia Raso, Marco Gaspari, Dora Maria Ceccarelli
    Abstract:

    Background Active infection by Bovine Papillomavirus type 2 (BPV-2) was documented for fifteen urinary bladder tumors in cattle. Two were diagnosed as papillary urothelial neoplasm of low malignant potential (PUNLMP), nine as papillary and four as invasive urothelial cancers.

  • pbmcs are additional sites of productive infection of Bovine Papillomavirus type 2
    Journal of General Virology, 2011
    Co-Authors: Sante Roperto, Valeria Russo, Iolanda Esposito, Chiara Urraro, Roberta De Luca, Giuseppe Borzacchiello, S Comazzi, Emilio Ciusani, Francesca Paolini, Aldo Venuti
    Abstract:

    Bovine Papillomavirus type 2 (BPV-2) is an oncogenic virus infecting both epithelial and mesenchymal cells. Its life cycle, similar to other Papillomaviruses (PVs), appears to be linked to epithelial differentiation. Human and Bovine PVs have been known to reside in a latent, episomal form in PBMCs; therefore, it is believed that blood cells, like all mesenchymal cells, function as non-permissive carriers. Here, for the first time in veterinary and comparative medicine, the BPV-2 E5 oncoprotein and the major structural L1 capsid protein, known to be expressed only in productive infections, were shown to occur in defined subsets of PBMCs. E5 oncoprotein was detected in sorted T- and B-cells as well as in monocytes by flow cytometry and Western blot analysis. However, CD4+ and CD8+ lymphocytes appeared to be the main circulating targets of the virus, thus possibly representing the most important reservoir of active BPV-2 in blood. L1 protein was identified by flow cytometry in a population of blood cells recognized as lymphocytes by morphological scatter properties. Western blot analysis was performed on lysates obtained from the sorted subpopulations of PBMCs and detected L1 protein in CD4+ and CD8+ cells only. Thus, this study showed that CD4+ and CD8+ lymphocytes are permissive for BPV-2 and are new, hitherto unknown sites of productive PV infection. In light of these observations, the life cycle of PVs needs to be revisited to gain novel insights into the epidemiology of BPV infection and the pathogenesis of related diseases.

  • Bovine Papillomavirus type 1 dna and e5 oncoprotein expression in water buffalo fibropapillomas
    Veterinary Pathology, 2009
    Co-Authors: O Silvestre, Giuseppe Borzacchiello, M S Campo, E A Gault, D Nava, G Iovane, Valentina Russo, D Vecchio, F Dausilio, Orlando Paciello
    Abstract:

    Papillomas and fibropapillomas may occur in the skin and in different organs in animals. Ten different genotypes of Bovine Papillomavirus (BPV) have been identified. BPV-1 through BPV-10 are all strictly species-specific, but BPV-1/2 may also infect other species such as equids, inducing fibroblastic tumors. BPV-1 and BPV-2 are associated with fibropapillomas in cattle; these tumors are formed by excessive proliferation of virus-infected dermal fibroblasts and epidermal keratinocytes. Nine water buffalo (Bubalus bubalis) were examined for the presence of multiple cutaneous and perivulvar tumors. Cutaneous and perivulvar fibropapillomatosis were confirmed histologically. Negative-stain transmission electron microscopic examination revealed Papillomavirus-like particles in the fibropa- pillomas, and papillomaviral DNA was also detected by the polymerase chain reaction. The amplified long control region (LCR) DNA sequence was identical to that of BPV-1. The BPV-1 E5 oncoprotein was strongly expressed in the tumor cells thus confirming a causal role of the virus. This article represents the first report of cutaneous, perivulvar, and vulvar fibropapilloma associated with BPV-1 infection in the water buffalo and describes another example of cross-species infection by BPV-1.

  • expression of platelet derived growth factor β receptor and Bovine Papillomavirus e5 and e7 oncoproteins in equine sarcoid
    Journal of Comparative Pathology, 2008
    Co-Authors: Giuseppe Borzacchiello, Sante Roperto, Valeria Russo, L Della Salda, F Roperto
    Abstract:

    Equine sarcoids are benign fibroblastic skin tumours that are recognized throughout the world. Infection with Bovine Papillomavirus (BPV) types 1 and 2 has been implicated as a major factor in disease development; however, the cellular mechanisms underlying fibroblast transformation remain poorly defined. The present study further characterizes aspects of the association with BPV in 15 equine sarcoids. BPV DNA was demonstrated in 12/15 tumours collected from different areas of Italy. Nine of these 12 tumours expressed the BPV oncoproteins E5 and E7, but these oncoproteins were not expressed by normal equine cells. The BPV E5 protein is known to bind to the platelet-derived growth factor-beta receptor (PDGF-betaR) and this molecule was expressed by 11 of the 12 sarcoids in which E5 was demonstrated. These findings add further weight to the theory that BPV and the PDGF-betaR may have a role in the pathogenesis of this disease.

  • Bovine Papillomavirus e5 oncoprotein binds to the activated form of the platelet derived growth factor beta receptor in naturally occurring Bovine urinary bladder tumours
    Oncogene, 2006
    Co-Authors: Giuseppe Borzacchiello, Valeria Russo, Flaviana Gentile, F Roperto, Aldo Venuti, Lucio Nitsch, M S Campo, Sante Roperto
    Abstract:

    Bovine Papillomavirus E5 oncoprotein binds to the activated form of the platelet-derived growth factor β receptor in naturally occurring Bovine urinary bladder tumours

Valeria Russo - One of the best experts on this subject based on the ideXlab platform.

  • Bovine Papillomavirus type 13 expression in the urothelial bladder tumours of cattle
    Transboundary and Emerging Diseases, 2016
    Co-Authors: Sante Roperto, Valeria Russo, Marita Georgia Riccardi, Leonardo Leonardi, Manuela Martano, Federica Corrado, F Roperto
    Abstract:

    Bovine Papillomavirus type 13 (BPV-13), a novel DeltaPapillomavirus, has been found associated with urothelial tumours of the urinary bladder of cattle grazing on lands infested with bracken fern. BPV-13 was detected in 28 of 39 urothelial tumours. Diagnosis was based on sequencing of L1 and E5 amplicons from tumour samples. The nucleotide sequences generated from these amplicons showed a 100% homology with the sequences of BPV-13 L1 and E5 DNA found in Brazil from a fibropapilloma of the ear in a cow and from equine sarcoids in two horses. GenBank accession number of our representative BPV-13 sequences is JQ798171.1. Furthermore, mRNA encoding BPV-13 E5 oncoprotein was also documented, and its expression was also shown by immunohistochemistry and immunofluorescence in the basal and suprabasal urothelial tumour cells. In twenty-three tumours, BPV-13 was simultaneously found with BPV-2, a DeltaPapillomavirus genus, species 4. The latter virus was detected by amplifying and sequencing a 154-bp-sized DNA fragment of BPV-2 E5. In addition, BPV-13 by itself was seen to be expressed in five BPV-2-negative urothelial tumours. This study shows that BPV-13 is present in urothelial tumour cells thus sharing biological properties with BPV-1 and BPV-2. Although further studies are needed, BPV-13 appears to be another worldwide infectious agent responsible for a distressing disease causing severe economic losses in cattle industry.

  • Bovine Papillomavirus type 2 bpv 2 e5 oncoprotein binds to the subunit d of the v1 atpase proton pump in naturally occurring urothelial tumors of the urinary bladder of cattle
    PLOS ONE, 2014
    Co-Authors: Sante Roperto, Valeria Russo, Iolanda Esposito, Marita Georgia Riccardi, Chiara Urraro, Roberta De Luca, Giuseppe Borzacchiello, Cinzia Raso, Marco Gaspari, Dora Maria Ceccarelli
    Abstract:

    Background Active infection by Bovine Papillomavirus type 2 (BPV-2) was documented for fifteen urinary bladder tumors in cattle. Two were diagnosed as papillary urothelial neoplasm of low malignant potential (PUNLMP), nine as papillary and four as invasive urothelial cancers.

  • productive infection of Bovine Papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes
    PLOS ONE, 2013
    Co-Authors: Sante Roperto, Valeria Russo, Ayhan Ozkul, Annunziata Corteggio, Aylin Sepicidincel, C Catoi, Iolanda Esposito, Marita Georgia Riccardi, Chiara Urraro, Roberta De Luca
    Abstract:

    Background Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of Bovine Papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring Papillomavirus-associated urothelial bladder tumors.

  • pbmcs are additional sites of productive infection of Bovine Papillomavirus type 2
    Journal of General Virology, 2011
    Co-Authors: Sante Roperto, Valeria Russo, Iolanda Esposito, Chiara Urraro, Roberta De Luca, Giuseppe Borzacchiello, S Comazzi, Emilio Ciusani, Francesca Paolini, Aldo Venuti
    Abstract:

    Bovine Papillomavirus type 2 (BPV-2) is an oncogenic virus infecting both epithelial and mesenchymal cells. Its life cycle, similar to other Papillomaviruses (PVs), appears to be linked to epithelial differentiation. Human and Bovine PVs have been known to reside in a latent, episomal form in PBMCs; therefore, it is believed that blood cells, like all mesenchymal cells, function as non-permissive carriers. Here, for the first time in veterinary and comparative medicine, the BPV-2 E5 oncoprotein and the major structural L1 capsid protein, known to be expressed only in productive infections, were shown to occur in defined subsets of PBMCs. E5 oncoprotein was detected in sorted T- and B-cells as well as in monocytes by flow cytometry and Western blot analysis. However, CD4+ and CD8+ lymphocytes appeared to be the main circulating targets of the virus, thus possibly representing the most important reservoir of active BPV-2 in blood. L1 protein was identified by flow cytometry in a population of blood cells recognized as lymphocytes by morphological scatter properties. Western blot analysis was performed on lysates obtained from the sorted subpopulations of PBMCs and detected L1 protein in CD4+ and CD8+ cells only. Thus, this study showed that CD4+ and CD8+ lymphocytes are permissive for BPV-2 and are new, hitherto unknown sites of productive PV infection. In light of these observations, the life cycle of PVs needs to be revisited to gain novel insights into the epidemiology of BPV infection and the pathogenesis of related diseases.

  • expression of platelet derived growth factor β receptor and Bovine Papillomavirus e5 and e7 oncoproteins in equine sarcoid
    Journal of Comparative Pathology, 2008
    Co-Authors: Giuseppe Borzacchiello, Sante Roperto, Valeria Russo, L Della Salda, F Roperto
    Abstract:

    Equine sarcoids are benign fibroblastic skin tumours that are recognized throughout the world. Infection with Bovine Papillomavirus (BPV) types 1 and 2 has been implicated as a major factor in disease development; however, the cellular mechanisms underlying fibroblast transformation remain poorly defined. The present study further characterizes aspects of the association with BPV in 15 equine sarcoids. BPV DNA was demonstrated in 12/15 tumours collected from different areas of Italy. Nine of these 12 tumours expressed the BPV oncoproteins E5 and E7, but these oncoproteins were not expressed by normal equine cells. The BPV E5 protein is known to bind to the platelet-derived growth factor-beta receptor (PDGF-betaR) and this molecule was expressed by 11 of the 12 sarcoids in which E5 was demonstrated. These findings add further weight to the theory that BPV and the PDGF-betaR may have a role in the pathogenesis of this disease.

F Roperto - One of the best experts on this subject based on the ideXlab platform.

  • Bovine Papillomavirus type 13 expression in the urothelial bladder tumours of cattle
    Transboundary and Emerging Diseases, 2016
    Co-Authors: Sante Roperto, Valeria Russo, Marita Georgia Riccardi, Leonardo Leonardi, Manuela Martano, Federica Corrado, F Roperto
    Abstract:

    Bovine Papillomavirus type 13 (BPV-13), a novel DeltaPapillomavirus, has been found associated with urothelial tumours of the urinary bladder of cattle grazing on lands infested with bracken fern. BPV-13 was detected in 28 of 39 urothelial tumours. Diagnosis was based on sequencing of L1 and E5 amplicons from tumour samples. The nucleotide sequences generated from these amplicons showed a 100% homology with the sequences of BPV-13 L1 and E5 DNA found in Brazil from a fibropapilloma of the ear in a cow and from equine sarcoids in two horses. GenBank accession number of our representative BPV-13 sequences is JQ798171.1. Furthermore, mRNA encoding BPV-13 E5 oncoprotein was also documented, and its expression was also shown by immunohistochemistry and immunofluorescence in the basal and suprabasal urothelial tumour cells. In twenty-three tumours, BPV-13 was simultaneously found with BPV-2, a DeltaPapillomavirus genus, species 4. The latter virus was detected by amplifying and sequencing a 154-bp-sized DNA fragment of BPV-2 E5. In addition, BPV-13 by itself was seen to be expressed in five BPV-2-negative urothelial tumours. This study shows that BPV-13 is present in urothelial tumour cells thus sharing biological properties with BPV-1 and BPV-2. Although further studies are needed, BPV-13 appears to be another worldwide infectious agent responsible for a distressing disease causing severe economic losses in cattle industry.

  • expression of platelet derived growth factor β receptor and Bovine Papillomavirus e5 and e7 oncoproteins in equine sarcoid
    Journal of Comparative Pathology, 2008
    Co-Authors: Giuseppe Borzacchiello, Sante Roperto, Valeria Russo, L Della Salda, F Roperto
    Abstract:

    Equine sarcoids are benign fibroblastic skin tumours that are recognized throughout the world. Infection with Bovine Papillomavirus (BPV) types 1 and 2 has been implicated as a major factor in disease development; however, the cellular mechanisms underlying fibroblast transformation remain poorly defined. The present study further characterizes aspects of the association with BPV in 15 equine sarcoids. BPV DNA was demonstrated in 12/15 tumours collected from different areas of Italy. Nine of these 12 tumours expressed the BPV oncoproteins E5 and E7, but these oncoproteins were not expressed by normal equine cells. The BPV E5 protein is known to bind to the platelet-derived growth factor-beta receptor (PDGF-betaR) and this molecule was expressed by 11 of the 12 sarcoids in which E5 was demonstrated. These findings add further weight to the theory that BPV and the PDGF-betaR may have a role in the pathogenesis of this disease.

  • Bovine Papillomavirus e5 oncoprotein binds to the activated form of the platelet derived growth factor beta receptor in naturally occurring Bovine urinary bladder tumours
    Oncogene, 2006
    Co-Authors: Giuseppe Borzacchiello, Valeria Russo, Flaviana Gentile, F Roperto, Aldo Venuti, Lucio Nitsch, M S Campo, Sante Roperto
    Abstract:

    Bovine Papillomavirus E5 oncoprotein binds to the activated form of the platelet-derived growth factor β receptor in naturally occurring Bovine urinary bladder tumours

  • presence of Bovine Papillomavirus type 2 dna and expression of the viral oncoprotein e5 in naturally occurring urinary bladder tumours in cows
    Journal of General Virology, 2003
    Co-Authors: Giuseppe Borzacchiello, Sante Roperto, F Roperto, Federica Poggiali, G Iovane, M L Marcante, Aldo Venuti
    Abstract:

    Samples of neoplastic and normal urothelium were obtained from cows originating from areas of southern Italy, a region in which chronic enzootic haematuria is endemic and bracken fern infestation is widespread. Specimens were analysed for Bovine Papillomavirus type 2 (BPV-2) DNA, BPV-2 E5 expression and telomerase activity. A total of 46 of 60 tumours and 17 of 34 normal bladder mucosa samples harboured BPV-2 DNA. Analysis of a subset of samples showed E5 protein expression and telomerase activity in tumour tissue only. No normal samples positive for BPV DNA showed E5 protein expression or telomerase activity, suggesting the presence of DNA in a latent state. Taken together, these data on naturally occurring Bovine bladder tumours corroborate the hypothesis of their virus origin.

  • Bovine Papillomavirus type 4 in oesophageal papillomas of cattle from the south of italy
    Journal of Comparative Pathology, 2003
    Co-Authors: Giuseppe Borzacchiello, Sante Roperto, Aldo Venuti, M S Campo, V Ambrosio, Federica Poggiali, E Tsirimonakis, F Roperto
    Abstract:

    Oesophageal papillomas are known to occur in cattle infected with Bovine Papillomavirus type 4 (BPV-4), and BPV-4 papillomas may undergo malignant progression in cattle that feed on bracken fern. In the south of Italy, where bracken fern is common, examination of 1133 slaughterhouse cattle aged 4-12 years revealed oesophageal lesions (single or multiple peduncuolated proliferations, or mucosal thickening) in 147 (13%). These two types of lesion were consistent with exophytic and inverted papilloma, respectively. BPV-4 was detected by polymerase chain reaction (PCR) analysis in >60% of the samples in which oesophageal papilloma was diagnosed histopathologically. Nucleotide sequencing of the PCR amplicons confirmed the presence of BPV-4 in the papillomas. This is the first report of such infections in a European country other than Britain.