The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform

Troy A. Alexander - One of the best experts on this subject based on the ideXlab platform.

  • Development of methodology based on commercialized SERS-active substrates for rapid discrimination of Poxviridae virions.
    Analytical Chemistry, 2008
    Co-Authors: Troy A. Alexander
    Abstract:

    Surface-enhanced Raman spectroscopy (SERS) can be made an attractive approach for identification of Raman-active compounds and biological materials (i.e., toxins, viruses, or intact bacterial cells/spores) through development of reproducible, spatially uniform SERS-active substrates. Recently, reproducible (from substrate-to-substrate), spatially homogeneous (over large areas) SERS-active substrates have been commercialized and are now available in the marketplace. We have utilized these patterned surfaces to acquire SERS spectral signatures of intact Bovine Papular Stomatitis, pseudocowpox, and Yaba monkey tumor viruses. Salient spectral signature features make it possible to discriminate among these genetically distinct Poxviridae−Chordopoxvirinae virions. In addition, partial least-squares, a multivariate calibration method, has been used to develop personal computer-borne algorithms useful for classification of unknown Parapoxvirus (e.g., Bovine Papular Stomatitis virus and pseudocowpox virus) samples...

  • Development of methodology based on commercialized SERS-active substrates for rapid discrimination of Poxviridae virions.
    Analytical chemistry, 2008
    Co-Authors: Troy A. Alexander
    Abstract:

    Surface-enhanced Raman spectroscopy (SERS) can be made an attractive approach for identification of Raman-active compounds and biological materials (i.e., toxins, viruses, or intact bacterial cells/spores) through development of reproducible, spatially uniform SERS-active substrates. Recently, reproducible (from substrate-to-substrate), spatially homogeneous (over large areas) SERS-active substrates have been commercialized and are now available in the marketplace. We have utilized these patterned surfaces to acquire SERS spectral signatures of intact Bovine Papular Stomatitis, pseudocowpox, and Yaba monkey tumor viruses. Salient spectral signature features make it possible to discriminate among these genetically distinct Poxviridae-Chordopoxvirinae virions. In addition, partial least-squares, a multivariate calibration method, has been used to develop personal computer-borne algorithms useful for classification of unknown Parapoxvirus (e.g., Bovine Papular Stomatitis virus and pseudocowpox virus) samples based solely on SERS spectral signatures. To our knowledge, this is the first report detailing application of these commercial-off-the-shelf (COTS) SERS-active substrates to identification of intact poxviruses.

Hiroshi Sentsui - One of the best experts on this subject based on the ideXlab platform.

  • Severe dermatitis on cattle teats caused by Bovine Papular Stomatitis virus
    The Veterinary record, 2009
    Co-Authors: Yasuo Inoshima, Takashi Nakane, Hiroshi Sentsui
    Abstract:

    We read with interest the letter by Leonard and others ( VR , January 10, 2009, vol 164, p 65) describing extensive proliferative scabby lesions on the teats of a dairy cow, where Bovine Papular Stomatitis virus (BPSV), a member of the genus Parapoxvirus , was unexpectedly identified by sequence

  • Characterization of parapoxviruses circulating among wild Japanese serows (Capricornis crispus).
    Microbiology and immunology, 2002
    Co-Authors: Yasuo Inoshima, Kenji Murakami, Hiroshi Sentsui
    Abstract:

    We antigenically and molecularly compared 5 parapoxvirus isolates and 7 viral DNA samples from clinical lesions of Japanese serows with 3 viruses from sheep and goats. All isolates from Japanese serows except one, Ishikawa-S, reacted with six monoclonal antibodies to orf virus (ORFV). Restriction endonuclease analysis using amplified viral DNA showed the ORFV-specific pattern in all samples except Ishikawa-S, which showed a Bovine Papular Stomatitis virus (BPSV)-specific pattern. Partial nucleotide sequences of the envelope genes were determined and those of all samples from Japanese serows and sheep except Ishikawa-S were completely identical and also had high identities with the goat virus. These findings suggest that parapoxvirus infection in Japanese serows might be mainly caused by ORFV and accidentally by BPSV. The envelope gene sequenced here seems to be conserved in Japanese ORFVs.

  • Genetic heterogeneity among parapoxviruses isolated from sheep, cattle and Japanese serows (Capricornis crispus).
    Journal of General Virology, 2001
    Co-Authors: Yasuo Inoshima, Kenji Murakami, Takashi Yokoyama, Hiroshi Sentsui
    Abstract:

    Standard strains of four parapoxviruses and seven unclassified Japanese strains isolated from sheep, cattle and wild Japanese serows (Capricornis crispus) were compared molecularly. Restriction fragment length polymorphism (RFLP) analysis of viral DNA, indirect immunofluorescence assays using monoclonal antibodies, partial nucleotide sequencing of the envelope gene, phylogenetic analysis and PCR–RFLP were carried out. These analyses revealed that the parapoxviruses were divided into four groups and the region sequenced in this study was highly conserved within each group. Each of the Japanese isolates was classified into one of these groups. These findings also indicated that parapoxvirus infections among wild Japanese serows seem to be caused by at least two different parapoxviruses, Bovine Papular Stomatitis virus and orf virus. The methods presented here are useful for genetic characterization and classification of parapoxviruses.

  • Detection and diagnosis of parapoxvirus by the polymerase chain reaction.
    Journal of virological methods, 2000
    Co-Authors: Yasuo Inoshima, Akira Morooka, Hiroshi Sentsui
    Abstract:

    The genus Parapoxvirus includes four members, Bovine Papular Stomatitis virus (BPSV), pseudocowpox virus (PCPV), orf virus (ORFV) and parapoxvirus of red deer in New Zealand (PVNZ). A set of primers for polymerase chain reaction (PCR) was designed to detect viral DNA from cells infected with each of the four parapoxviruses. The set of primers resulted in the amplification of appropriately sized products from cells infected with BPSV, PCPV, ORFV and PVNZ, respectively. The PCR method was applied for the detection of seven field isolates of parapoxvirus from cattle, sheep and free-ranging wild Japanese serows. The expected size of DNA was amplified from cells infected with each of the seven isolates. No specific PCR products were detected from vaccinia virus-, fowlpox virus- and mock-infected cells. Moreover, by a semi-nested PCR with an inner primer and Southern blot analysis, viral DNA was detected from lesions of clinically affected cattle, sheep and Japanese serows. These results suggested that the PCR method used in this study was specific for the detection of parapoxviruses and thus useful for diagnosis of parapoxvirus infections, especially in discrimination from diseases with similar clinical symptoms.

Colin Mcinnes - One of the best experts on this subject based on the ideXlab platform.

  • Severe oesophagitis in an adult bull caused by Bovine Papular Stomatitis virus.
    The Veterinary record, 2011
    Co-Authors: Sonja Jeckel, Colin Mcinnes, Ann R. Wood, David J. Everest, Cornelia Bidewell, Julian Dare, A. Schock
    Abstract:

    INFECTIONS with Bovine Papular Stomatitis virus (BPSV) are most commonly reported in young cattle, usually with mild lesions in the mouth and on the nose (Andrews 2004). There is a zoonotic risk, as described in a recent letter to Veterinary Record (Holmes and others 2011). We wish to report in more detail an unusually severe and extensive infection with BPSV affecting an adult Limousin bull, previously mentioned briefly in the VLA disease surveillance report for February 2009 ( VR , April 25, 2009, vol 164, p 515). The animal was one of a group of four two-and-a-half-year-old bulls, which developed illthrift shortly after housing following their working season on extensive marshland grazing. The bulls …

  • Zoonotic transmission of Bovine Papular Stomatitis virus.
    The Veterinary record, 2011
    Co-Authors: Paul Holmes, Colin Mcinnes, Ann R. Wood, Moyna Richey, A. Schock, Esme Wilson, Paul Verney
    Abstract:

    Bovine Papular Stomatitis virus (BPSV) is usually considered a mild disease in calves and is rarely associated with severe disease problems. We would like to report a case in which this virus was acquired from a calf and caused severe disease in one of its handlers. A six-week-old Aberdeen Angus suckler calf with chronic scour had been treated by the owner and the owner's assistant for over a week before it was euthanased and submitted to Animal Health and Veterinary Laboratories Agency (AHVLA) – Shrewsbury under Defra's cattle scanning surveillance programme. …

  • the genome of pseudocowpoxvirus comparison of a reindeer isolate and a reference strain
    Journal of General Virology, 2010
    Co-Authors: Maria Hautaniemi, Andrew A. Mercer, Norihito Ueda, Jarno Tuimala, Juhani Lahdenpera, Colin Mcinnes
    Abstract:

    Parapoxviruses (PPV), of the family Poxviridae, cause a pustular cutaneous disease in sheep and goats (orf virus, ORFV) and cattle (pseudocowpoxvirus, PCPV and Bovine Papular Stomatitis virus, BPSV). Here, we present the first genomic sequence of a reference strain of PCPV (VR634) along with the genomic sequence of a PPV (F00.120R) isolated in Finland from reindeer (Rangifer tarandus tarandus). The F00.120R and VR634 genomes are 135 and 145 kb in length and contain 131 and 134 putative genes, respectively, with their genome organization being similar to that of other PPVs. The predicted proteins of F00.120R and VR634 have an average amino acid sequence identity of over 95 %, whereas they share only 88 and 73 % amino acid identity with the ORFV and BPSV proteomes, respectively. The most notable differences were found near the genome termini. F00.120R lacks six and VR634 lacks three genes seen near the right terminus of other PPVs. Four genes at the left end of F00.120R and one in the middle of both genomes appear to be fragmented paralogues of other genes within the genome. VR634 has larger than expected inverted terminal repeats possibly as a result of genomic rearrangements. The high G+C content (64 %) of these two viruses along with amino acid sequence comparisons and whole genome phylogenetic analyses confirm the classification of PCPV as a separate species within the genus Parapoxvirus and verify that the virus responsible for an outbreak of contagious Stomatitis in reindeer over the winter of 1999–2000 can be classified as PCPV.

  • Conservation and variation of the parapoxvirus GM-CSF-inhibitory factor (GIF) proteins
    Journal of General Virology, 2009
    Co-Authors: David Deane, Norihito Ueda, Lyn M. Wise, Stephen B. Fleming, Ann R. Wood, Ann Percival, C. Jepson, Neil F. Inglis, Colin Mcinnes
    Abstract:

    The GIF protein of orf virus (ORFV) binds and inhibits the ovine cytokines granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). An equivalent protein has so far not been found in any of the other poxvirus genera and we therefore investigated whether it was conserved in the parapoxviruses. The corresponding genes from both the Bovine-specific pseudocowpox virus (PCPV) and Bovine Papular Stomatitis virus (BPSV) were cloned and sequenced. The predicted amino acid sequences of the PCPV and BPSV proteins shared 88 and 37 % identity, respectively, with the ORFV protein. Both retained the six cysteine residues and the WSXWS-like motif that are required for biological activity of the ORFV protein. However, an analysis of the biological activity of the two recombinant proteins revealed that, whilst the PCPV GIF protein bound to both ovine and Bovine GM-CSF and IL-2 with very similar binding affinities to the ORFV GIF protein, no GM-CSF- or IL-2-binding activity was found for the BPSV protein.

  • Unusual Bovine Papular Stomatitis virus infection in a British dairy cow.
    The Veterinary record, 2009
    Co-Authors: Den Leonard, Colin Mcinnes, Ann R. Wood, A. Otter, David J. Everest, A. Schock
    Abstract:

    We wish to report an unusual presentation of Bovine Papular Stomatitis virus (BPSV) infection of a single cow’s teat, which was the only clinically detected manifestation of this infection in a dairy herd. The affected animal was a five-year-old Holstein in its third lactation with an average

A. De La Concha-bermejillo - One of the best experts on this subject based on the ideXlab platform.

  • Genetic characterization of orf viruses isolated from various ruminant species of a zoo.
    Veterinary microbiology, 2004
    Co-Authors: J. Guo, James M. Rasmussen, Arno Wünschmann, A. De La Concha-bermejillo
    Abstract:

    In the present study, an outbreak of proliferative dermatitis in musk ox (Ovibos moschatus), Sichuan takin (Budorcas taxicolor tibetana) and domestic Shetland sheep (Ovis aries) in a zoo is described. Skin lesions consisted of severe, persistent, multifocal, proliferative dermatitis in musk ox, and mild, transient, focal, dermatitis in the Sichuan takin and Shetland sheep. Parapoxviruses were isolated from skin lesions, and characterized by restriction enzyme analysis and partial gene sequencing. The results of this investigation indicate that the outbreak of proliferative dermatitis was due to infection by a single parapoxvirus, which is genetically closely related to other orf virus (ORFV) strains but distant to Bovine Papular Stomatitis virus (BPSV) and pseudocowpox virus (PCPV).

  • Genomes of the Parapoxviruses Orf Virus and Bovine Papular Stomatitis Virus
    Journal of virology, 2004
    Co-Authors: G. Delhon, A. De La Concha-bermejillo, E. R. Tulman, Gerald F. Kutish, Claudio L. Afonso, Howard D. Lehmkuhl, M. E. Piccone, Daniel L. Rock
    Abstract:

    Bovine Papular Stomatitis virus (BPSV) and orf virus (ORFV), members of the genus Parapoxvirus of the Poxviridae, are etiologic agents of worldwide diseases affecting cattle and small ruminants, respectively. Here we report the genomic sequences and comparative analysis of BPSV strain BV-AR02 and ORFV strains OV-SA00, isolated from a goat, and OV-IA82, isolated from a sheep. Parapoxvirus (PPV) BV-AR02, OV-SA00, and OV-IA82 genomes range in size from 134 to 139 kbp, with an average nucleotide composition of 64% G+C. BPSV and ORFV genomes contain 131 and 130 putative genes, respectively, and share colinearity over 127 genes, 88 of which are conserved in all characterized chordopoxviruses. BPSV and ORFV contain 15 and 16 open reading frames (ORFs), respectively, which lack similarity to other poxvirus or cellular proteins. All genes with putative roles in pathogenesis, including a vascular endothelial growth factor (VEGF)-like gene, are present in both viruses; however, BPSV contains two extra ankyrin repeat genes absent in ORFV. Interspecies sequence variability is observed in all functional classes of genes but is highest in putative virulence/host range genes, including genes unique to PPV. At the amino acid level, OV-SA00 is 94% identical to OV-IA82 and 71% identical to BV-AR02. Notably, ORFV 006/132, 103, 109, 110, and 116 genes (VEGF, homologues of vaccinia virus A26L, A33R, and A34R, and a novel PPV ORF) show an unusual degree of intraspecies variability. These genomic differences are consistent with the classification of BPSV and ORFV as two PPV species. Compared to other mammalian chordopoxviruses, PPV shares unique genomic features with molluscum contagiosum virus, including a G+C-rich nucleotide composition, three orthologous genes, and a paucity of nucleotide metabolism genes. Together, these data provide a comparative view of PPV genomics.

  • Characterization of a North American orf virus isolated from a goat with persistent, proliferative dermatitis.
    Virus research, 2003
    Co-Authors: J. Guo, Z. Zhang, John F. Edwards, R.w. Ermel, Charles A. Taylor, A. De La Concha-bermejillo
    Abstract:

    The characterization of an orf virus (OV) isolated from skin lesions of a goat kid with severe, persistent, proliferative dermatitis, and designated orf virus-San Angelo 2000 (OV-SA00) strain, is described. The identity of OV-SA00 was confirmed by a combination of methods, including electron microscopy, amplification of specific fragments of viral DNA by polymerase chain reaction, restriction enzyme analysis of viral DNA and gene sequencing. Restriction endonuclease analyses of viral DNA and the protein profile studied by Western blot revealed differences between OV-SA00 strain and the profiles of other OV strains that have been published. The restriction enzyme profile of OV-SA00 was also different from the orf virus vaccine (OV-V) strain used to vaccinate this kid. Comparison of the nucleotide and deduced amino acid sequences indicated that OV-SA00 is closely related to OV-V strain, the Scottish OV strains orf11 and MRI Scab, and the human OV-CE/Shoe strain and more distant to Bovine Papular Stomatitis virus (BPSV) reference strain and the pseudocowpox virus (PCPV)-MNV/Till strain. These results indicate that OV-SA00 is a strain of OV rather than a different parapoxvirus. Further studies are necessary to determine if the severity of orf-induced lesions in this goat kid was the result of individual host susceptibility factors.

Yasuo Inoshima - One of the best experts on this subject based on the ideXlab platform.

  • Successful measures to prevent the spread of Bovine Papular Stomatitis in a dairy farm.
    The Journal of veterinary medical science, 2020
    Co-Authors: Masaki Kato, Kaori Shimizu, Daiki Kawashima, Yui Ito, Osamu Yamamoto, Hiromi Nakajima, Takuya Oizumi, Yasuo Inoshima
    Abstract:

    Nasal papules and oral ulcers were observed in calves that were group-housed at a dairy farm. The calves were diagnosed with Bovine Papular Stomatitis (BPS) due to parapoxvirus (PPV) infection based on virologic examinations using polymerase chain reaction to detect PPV. To prevent the spread of BPS, we isolated the affected calves, made procedural changes so that the affected herd was managed after the healthy herd, disinfected the bedding with slaked lime, disinfected the stalls and fences with invert soap, and changed the animals' feed to soft grass which does not damage the oral cavity. As a result, we succeeded in control the infection quickly.

  • serological and epidemiological studies of parapoxvirus infection in cattle in ishikawa prefecture
    Jarq-japan Agricultural Research Quarterly, 2011
    Co-Authors: Mika Ito, Toshiaki Murakami, Yuji Hayakawa, Eiichi Shintani, Yasuo Inoshima
    Abstract:

    A survey of antibodies against parapoxvirus species Bovine Papular Stomatitis virus (BPSV) was conducted by agar gel immunodiffusion (AGID) test on 750 serum samples collected from cattle in Ishikawa Prefecture, Japan, in 2007. On farms, 0-21.9% dairy cattle and 8.3-33.3% beef cattle were seropositive, but the difference was not significant. The positive reaction rate was significantly higher in dairy cattle reared on public pastures (33.3-82.4%) than in dairy and beef cattle reared on farms. Among dairy cattle on farms, the positive reaction rate was significantly higher in cattle reared on public pastures and in cattle introduced from other prefectures than in cattle that were neither reared on pastures nor introduced from other prefectures. The same serum samples were tested for antibodies against orf virus (ORFV) by AGID, and the positive reaction rate for ORFV was almost the same or less than that for BPSV, and the immunodiffusion line on the gel was weaker for ORFV. There was no correlation between positive reaction rates in cattle and grazing on public pastures that geographically overlap with the habitat of Japanese serows (Capricornis crispus), which are also hosts of parapoxvirus. These results suggest that the risk of BPSV infection is higher for dairy cattle reared on pastures than on farms, but the risk of infection due to grazing on pastures inhabited by Japanese serows seems to be low.

  • Severe dermatitis on cattle teats caused by Bovine Papular Stomatitis virus
    The Veterinary record, 2009
    Co-Authors: Yasuo Inoshima, Takashi Nakane, Hiroshi Sentsui
    Abstract:

    We read with interest the letter by Leonard and others ( VR , January 10, 2009, vol 164, p 65) describing extensive proliferative scabby lesions on the teats of a dairy cow, where Bovine Papular Stomatitis virus (BPSV), a member of the genus Parapoxvirus , was unexpectedly identified by sequence

  • Characterization of parapoxviruses circulating among wild Japanese serows (Capricornis crispus).
    Microbiology and immunology, 2002
    Co-Authors: Yasuo Inoshima, Kenji Murakami, Hiroshi Sentsui
    Abstract:

    We antigenically and molecularly compared 5 parapoxvirus isolates and 7 viral DNA samples from clinical lesions of Japanese serows with 3 viruses from sheep and goats. All isolates from Japanese serows except one, Ishikawa-S, reacted with six monoclonal antibodies to orf virus (ORFV). Restriction endonuclease analysis using amplified viral DNA showed the ORFV-specific pattern in all samples except Ishikawa-S, which showed a Bovine Papular Stomatitis virus (BPSV)-specific pattern. Partial nucleotide sequences of the envelope genes were determined and those of all samples from Japanese serows and sheep except Ishikawa-S were completely identical and also had high identities with the goat virus. These findings suggest that parapoxvirus infection in Japanese serows might be mainly caused by ORFV and accidentally by BPSV. The envelope gene sequenced here seems to be conserved in Japanese ORFVs.

  • Genetic heterogeneity among parapoxviruses isolated from sheep, cattle and Japanese serows (Capricornis crispus).
    Journal of General Virology, 2001
    Co-Authors: Yasuo Inoshima, Kenji Murakami, Takashi Yokoyama, Hiroshi Sentsui
    Abstract:

    Standard strains of four parapoxviruses and seven unclassified Japanese strains isolated from sheep, cattle and wild Japanese serows (Capricornis crispus) were compared molecularly. Restriction fragment length polymorphism (RFLP) analysis of viral DNA, indirect immunofluorescence assays using monoclonal antibodies, partial nucleotide sequencing of the envelope gene, phylogenetic analysis and PCR–RFLP were carried out. These analyses revealed that the parapoxviruses were divided into four groups and the region sequenced in this study was highly conserved within each group. Each of the Japanese isolates was classified into one of these groups. These findings also indicated that parapoxvirus infections among wild Japanese serows seem to be caused by at least two different parapoxviruses, Bovine Papular Stomatitis virus and orf virus. The methods presented here are useful for genetic characterization and classification of parapoxviruses.