The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Alina D. Zamfir - One of the best experts on this subject based on the ideXlab platform.
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high resolution mass spectrometry provides novel insights into the ganglioside pattern of Brain cavernous Hemangioma
Analytical Biochemistry, 2020Co-Authors: Raluca Ica, Zeljka Vukelic, Anca Simulescu, Mirela Sarbu, Cristian V A Munteanu, Alina D. ZamfirAbstract:In this study we have optimized nanoelectrospray ionization (nanoESI) high resolution mass spectrometry (HR MS) performed on Orbitrap instrument in the negative ion mode for the determination of the composition and structure of gangliosides extracted from human Brain cavernous Hemangioma. The optimized HR MS platform, allowed the discrimination of 62 ions, corresponding to 52 different ganglioside species, which represents roughly twice the number of species existing in the current inventory of human Brain Hemangioma-associated gangliosides. The experiments revealed a ganglioside pattern dominated by GD-type of structures as well as an elevated incidence of species characterized by a low degree of sialylation and short glycan chains, including asialo GA1 (d18:1/18:0), which offer a new perspective upon the ganglioside composition in this benign tumor. Many of the structures are characteristic for this type of tumor only and are to be considered in further investigations for their potential use in early Brain Hemangioma diagnosis based on molecular markers. The detailed fragmentation analysis performed by collision-induced dissociation (CID) tandem MS provided information of structural elements related to the glycan core and ceramide moiety, which confirmed the molecular configuration of GD3 (d18:1/24:1) and GD3 (d18:1/24:2) species with potential biomarker role.
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Determination of ganglioside composition and structure in human Brain Hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Željka Vukelić, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O -Ac-GD2 and one modified O -Ac-GM4 gangliosides were observed. This aspect suggests that these O -acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS^2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor. Human Brain biomarker discovery by advanced chipbased nanoelectrospray mass spectrometry
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determination of ganglioside composition and structure in human Brain Hemangioma by chip based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Svjetlana Kalanjbognar, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O-Ac-GD2 and one modified O-Ac-GM4 gangliosides were observed. This aspect suggests that these O-acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor.
Catalin Schiopu - One of the best experts on this subject based on the ideXlab platform.
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nanoelectrospray ionization
2016Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Allna Serb, Adna D. Zamfir, C. SchiopuAbstract:Determination of ganglioside composition and structure in human Brain Hemangioma by chip-base
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Determination of ganglioside composition and structure in human Brain Hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Željka Vukelić, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O -Ac-GD2 and one modified O -Ac-GM4 gangliosides were observed. This aspect suggests that these O -acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS^2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor. Human Brain biomarker discovery by advanced chipbased nanoelectrospray mass spectrometry
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determination of ganglioside composition and structure in human Brain Hemangioma by chip based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Svjetlana Kalanjbognar, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O-Ac-GD2 and one modified O-Ac-GM4 gangliosides were observed. This aspect suggests that these O-acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor.
Florina Capitan - One of the best experts on this subject based on the ideXlab platform.
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nanoelectrospray ionization
2016Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Allna Serb, Adna D. Zamfir, C. SchiopuAbstract:Determination of ganglioside composition and structure in human Brain Hemangioma by chip-base
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Determination of ganglioside composition and structure in human Brain Hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Željka Vukelić, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O -Ac-GD2 and one modified O -Ac-GM4 gangliosides were observed. This aspect suggests that these O -acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS^2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor. Human Brain biomarker discovery by advanced chipbased nanoelectrospray mass spectrometry
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determination of ganglioside composition and structure in human Brain Hemangioma by chip based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Svjetlana Kalanjbognar, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O-Ac-GD2 and one modified O-Ac-GM4 gangliosides were observed. This aspect suggests that these O-acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor.
Corina Flangea - One of the best experts on this subject based on the ideXlab platform.
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nanoelectrospray ionization
2016Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Allna Serb, Adna D. Zamfir, C. SchiopuAbstract:Determination of ganglioside composition and structure in human Brain Hemangioma by chip-base
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Determination of ganglioside composition and structure in human Brain Hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Željka Vukelić, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O -Ac-GD2 and one modified O -Ac-GM4 gangliosides were observed. This aspect suggests that these O -acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS^2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor. Human Brain biomarker discovery by advanced chipbased nanoelectrospray mass spectrometry
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determination of ganglioside composition and structure in human Brain Hemangioma by chip based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Svjetlana Kalanjbognar, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O-Ac-GD2 and one modified O-Ac-GM4 gangliosides were observed. This aspect suggests that these O-acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor.
Eugen Sisu - One of the best experts on this subject based on the ideXlab platform.
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nanoelectrospray ionization
2016Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Allna Serb, Adna D. Zamfir, C. SchiopuAbstract:Determination of ganglioside composition and structure in human Brain Hemangioma by chip-base
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Determination of ganglioside composition and structure in human Brain Hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Željka Vukelić, Svjetlana Kalanj-bognar, Eugen Sisu, Michael Przybylski, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O -Ac-GD2 and one modified O -Ac-GM4 gangliosides were observed. This aspect suggests that these O -acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS^2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor. Human Brain biomarker discovery by advanced chipbased nanoelectrospray mass spectrometry
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determination of ganglioside composition and structure in human Brain Hemangioma by chip based nanoelectrospray ionization tandem mass spectrometry
Analytical and Bioanalytical Chemistry, 2009Co-Authors: Catalin Schiopu, Corina Flangea, Florina Capitan, Alina Serb, Eugen Sisu, Michael Przybylski, Zeljka Vukelic, Svjetlana Kalanjbognar, Alina D. ZamfirAbstract:We report here on a preliminary investigation of ganglioside composition and structure in human Hemangioma, a benign tumor in the frontal cortex (HFC) in comparison to normal frontal cortex (NFC) tissue using for the first time advanced mass spectrometric methods based on fully automated chip-nanoelectrospray (nanoESI) high-capacity ion trap (HCT) and collision-induced dissociation (CID). The high ionization efficiency, sensitivity and reproducibility provided by the chip-nanoESI approach allowed for a reliable MS-based ganglioside comparative assay. Unlike NFC, ganglioside mixture extracted from HFC was found dominated by species of short glycan chains exhibiting lower overall sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected. Interestingly, none of these trisialylated forms was detected in NFC, suggesting that such components might selectively be associated with HFC. Unlike the case of previously investigated high malignancy gliosarcoma, in HFC one modified O-Ac-GD2 and one modified O-Ac-GM4 gangliosides were observed. This aspect suggests that these O-acetylated structures could be associated with cerebral tumors having reduced malignancy grade. Fragmentation analysis by CID in MS2 mode using as precursors the ions corresponding to GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data corroborating for the first time the presence of the common GT1a and GT1b isomers and the incidence of unusual GT1c and GT1d glycoforms in Brain Hemangioma tumor.