The Experts below are selected from a list of 213 Experts worldwide ranked by ideXlab platform

Tizhong Shan - One of the best experts on this subject based on the ideXlab platform.

  • Breed Difference and regulatory role of crtc3 in porcine intramuscular adipocyte
    Animal Genetics, 2020
    Co-Authors: Jing Liu, Qiuyun Nong, J Wang, Wei Chen, Wenjing You, J Xie, Y Wang, Tizhong Shan
    Abstract:

    The cAMP responsive element binding protein (CREB)-regulated transcription coactivator 3 (CRTC3) is a member of the CRTC protein family and plays an important role in energy metabolism. The aim of this study was to determine if the expression of porcine CRTC3 is related to intramuscular fat (IMF) deposition and meat quality in Heigai pigs (a local fatty Breed in China) and Duroc × Landrace × Yorkshire (DLY) pigs (a lean crossbred pig widely cultured in China). In addition, the effect of ectopic expression of CRTC3 on gene expression in porcine IMF adipocytes was also examined. Our results showed that Heigai pigs had lower lean percentage, thicker back fat thickness and smaller loin muscle area than DLY pigs. Compared with DLY pigs, Heigai pigs had higher marbling scores, better meat color and higher IMF contents and triglyceride concentrations. Higher levels of oxidative metabolic enzyme and expression of the slow oxidative muscle fiber-related genes were observed in longissimus dorsi muscle and psoas major muscle (P < 0.05) from Heigai pigs. Notably, CRTC3 and adipocyte-specific marker genes were highly expressed in muscle tissues of Heigai pigs. The expression of lipolysis-related genes ATGL and HSL were lower in Heigai muscles. Moreover, forced expression of CRTC3 promoted lipid accumulation and increased the expression of PPARγ, C/EBPα, leptin and FABP4 (P < 0.05), whereas it decreased the expression of ATGL and HSL in IMF adipocytes. These results suggest that CRTC3 expression is associated with lipid accumulation and IMF deposition in pigs.

  • Breed Difference of porcine Sirtuin 1, adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL)
    Livestock Science, 2013
    Co-Authors: Yang Ren, Jing Huang, Tizhong Shan, Xihong Zhou, Xinxia Wang, Yizhen Wang
    Abstract:

    Abstract In the current study, we investigated the Breed Difference of Sirt1 gene expression in liver and pancreas between Jinhua pigs (a local fatty Breed of China) and Landrace (a leaner Breed). In addition, the gene expression of lipid metabolism enzymes adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) has also been examined. Results showed that the serum triglyceride (TG), and total cholesterol (TCHO) concentration of Jinhua pigs were greater than Landrace while the serum glycerol in Jinhua pigs was lower at the age of 180 d. The serum leptin concentration was also greater in Jinhua pigs. Compared with Landrace, the mRNA level and protein abundance of Sirt1 were lower both in liver and pancreas in Jinhua pigs. Consistent with Sirt1 expression, in liver and pancreas, the mRNA expression of lipolitic enzymes ATGL and HSL was always lower in Jinhua pigs than Landrace respectively. Furthermore, similar results were found in 60 d pigs. The mRNA levels of Sirt1, ATGL and HSL were decreased in 60 d Jinhua pigs comparatively. These results indicated that porcine Sirt1, as well as ATGL and HSL gene expressions are different between fatty and leaner pigs. This will provide useful information for better known of Sirt1 function in fat deposition.

  • Breed Difference and regulation of the porcine Sirtuin 1 by insulin.
    Journal of animal science, 2010
    Co-Authors: Tizhong Shan, Yang Ren, Yanyan Liu, Lingjun Zhu, Yizhen Wang
    Abstract:

    Sirtuin 1 (Sirt1) plays an important role in fat metabolism. In the current study, we examined the Breed Differences in Sirt1 between Jinhua pigs (a fatty Breed of China) and Landrace pigs (a leaner Breed). In addition, the effect of insulin on the gene expression of Sirt1 and the major lipase, adipose triglyceride lipase (ATGL), and hormone-sensitive lipase (HSL) in fat metabolism was also studied in vitro. Compared with the Landrace pigs, the BW of Jinhua pigs was less (P < 0.01), whereas the body fat content were greater (P < 0.01). The protein content and the mRNA abundance of Sirt1 in Jinhua pigs were less (P < 0.01) in subcutaneous adipose tissues compared with the Landrace pigs. Likewise, the mRNA abundance of ATGL and HSL were also less (P < 0.01) in Jinhua pigs. In vitro, treatment with a different dose of insulin (10, 50 and 100 nM) decreased (P < 0.01) glycerol release and the mRNA abundance of Sirt1, ATGL, and HSL in porcine adipocytes. Likewise, treatment with 50 nM insulin for 24 and 48 h also decreased (P < 0.05) glycerol release and the expression of Sirt1, ATGL, and HSL in porcine adipocytes. Furthermore, insulin and Sirt1-specific small interfering RNA treatment decreased (P < 0.01) the expression of Sirt1, ATGL, and HSL compared with the control or insulin treatment. These results indicate that insulin may regulate transcription of Sirt1, ATGL, and HSL in porcine adipocytes and provide information for manipulating these gene expressions in regulating fat metabolism in pigs.

  • Breed Difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα
    Animal Genetics, 2009
    Co-Authors: Tizhong Shan, T. Wu, Y. Reng, Yizhen Wang
    Abstract:

    : Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are major novel triglyceride lipases in animals. The aim of this study was to determine if there are Differences in the porcine ATGL (pATGL) and HSL genes between Jinhua pigs (a fatty Breed) and Landrace pigs (a leaner Breed). In addition, the effect of TNFalpha and pATGL-specific siRNA (pATGL-siRNA) on the expression of pATGL and HSL in porcine adipocytes was also examined. Compared with Landrace pigs, the body weight (BW) of Jinhua pigs was lower (P < 0.01), while intramuscular fat content (in the longissimus dorsi muscle), as well as the back fat thickness and body fat content were higher (P < 0.01). The expression of pATGL and HSL mRNA in Jinhua pigs was lower (P < 0.01) in subcutaneous adipose tissue, and greater (P < 0.01) in longissimus dorsi muscle compared with Landrace pigs. In vitro treatment of porcine adipocytes with TNFalpha decreased (P < 0.01) the glycerol release and the gene expression of pATGL, HSL and PPARgamma in porcine adipocytes. Furthermore, transfection with pATGL-siRNA significantly decreased (P < 0.01) the expression of pATGL, while it had no effect on the expression of HSL. Treatment with 25 ng/ml TNFalpha in conjunction with pATGL-siRNA significantly decreased (P < 0.01) the expression of pATGL and HSL in cultured porcine adipocytes. These results provide useful information to further the understanding of the function of pATGL and HSL in porcine lipid metabolism, which should be applicable to the regulation of fat deposition and improvement of meat quality.

Yizhen Wang - One of the best experts on this subject based on the ideXlab platform.

  • Breed Difference of porcine Sirtuin 1, adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL)
    Livestock Science, 2013
    Co-Authors: Yang Ren, Jing Huang, Tizhong Shan, Xihong Zhou, Xinxia Wang, Yizhen Wang
    Abstract:

    Abstract In the current study, we investigated the Breed Difference of Sirt1 gene expression in liver and pancreas between Jinhua pigs (a local fatty Breed of China) and Landrace (a leaner Breed). In addition, the gene expression of lipid metabolism enzymes adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) has also been examined. Results showed that the serum triglyceride (TG), and total cholesterol (TCHO) concentration of Jinhua pigs were greater than Landrace while the serum glycerol in Jinhua pigs was lower at the age of 180 d. The serum leptin concentration was also greater in Jinhua pigs. Compared with Landrace, the mRNA level and protein abundance of Sirt1 were lower both in liver and pancreas in Jinhua pigs. Consistent with Sirt1 expression, in liver and pancreas, the mRNA expression of lipolitic enzymes ATGL and HSL was always lower in Jinhua pigs than Landrace respectively. Furthermore, similar results were found in 60 d pigs. The mRNA levels of Sirt1, ATGL and HSL were decreased in 60 d Jinhua pigs comparatively. These results indicated that porcine Sirt1, as well as ATGL and HSL gene expressions are different between fatty and leaner pigs. This will provide useful information for better known of Sirt1 function in fat deposition.

  • Breed Difference and regulation of the porcine Sirtuin 1 by insulin.
    Journal of animal science, 2010
    Co-Authors: Tizhong Shan, Yang Ren, Yanyan Liu, Lingjun Zhu, Yizhen Wang
    Abstract:

    Sirtuin 1 (Sirt1) plays an important role in fat metabolism. In the current study, we examined the Breed Differences in Sirt1 between Jinhua pigs (a fatty Breed of China) and Landrace pigs (a leaner Breed). In addition, the effect of insulin on the gene expression of Sirt1 and the major lipase, adipose triglyceride lipase (ATGL), and hormone-sensitive lipase (HSL) in fat metabolism was also studied in vitro. Compared with the Landrace pigs, the BW of Jinhua pigs was less (P < 0.01), whereas the body fat content were greater (P < 0.01). The protein content and the mRNA abundance of Sirt1 in Jinhua pigs were less (P < 0.01) in subcutaneous adipose tissues compared with the Landrace pigs. Likewise, the mRNA abundance of ATGL and HSL were also less (P < 0.01) in Jinhua pigs. In vitro, treatment with a different dose of insulin (10, 50 and 100 nM) decreased (P < 0.01) glycerol release and the mRNA abundance of Sirt1, ATGL, and HSL in porcine adipocytes. Likewise, treatment with 50 nM insulin for 24 and 48 h also decreased (P < 0.05) glycerol release and the expression of Sirt1, ATGL, and HSL in porcine adipocytes. Furthermore, insulin and Sirt1-specific small interfering RNA treatment decreased (P < 0.01) the expression of Sirt1, ATGL, and HSL compared with the control or insulin treatment. These results indicate that insulin may regulate transcription of Sirt1, ATGL, and HSL in porcine adipocytes and provide information for manipulating these gene expressions in regulating fat metabolism in pigs.

  • Breed Difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα
    Animal Genetics, 2009
    Co-Authors: Tizhong Shan, T. Wu, Y. Reng, Yizhen Wang
    Abstract:

    : Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are major novel triglyceride lipases in animals. The aim of this study was to determine if there are Differences in the porcine ATGL (pATGL) and HSL genes between Jinhua pigs (a fatty Breed) and Landrace pigs (a leaner Breed). In addition, the effect of TNFalpha and pATGL-specific siRNA (pATGL-siRNA) on the expression of pATGL and HSL in porcine adipocytes was also examined. Compared with Landrace pigs, the body weight (BW) of Jinhua pigs was lower (P < 0.01), while intramuscular fat content (in the longissimus dorsi muscle), as well as the back fat thickness and body fat content were higher (P < 0.01). The expression of pATGL and HSL mRNA in Jinhua pigs was lower (P < 0.01) in subcutaneous adipose tissue, and greater (P < 0.01) in longissimus dorsi muscle compared with Landrace pigs. In vitro treatment of porcine adipocytes with TNFalpha decreased (P < 0.01) the glycerol release and the gene expression of pATGL, HSL and PPARgamma in porcine adipocytes. Furthermore, transfection with pATGL-siRNA significantly decreased (P < 0.01) the expression of pATGL, while it had no effect on the expression of HSL. Treatment with 25 ng/ml TNFalpha in conjunction with pATGL-siRNA significantly decreased (P < 0.01) the expression of pATGL and HSL in cultured porcine adipocytes. These results provide useful information to further the understanding of the function of pATGL and HSL in porcine lipid metabolism, which should be applicable to the regulation of fat deposition and improvement of meat quality.

Ruqian Zhao - One of the best experts on this subject based on the ideXlab platform.

  • Chicken FTO gene: tissue-specific expression, brain distribution, Breed Difference and effect of fasting.
    Comparative biochemistry and physiology. Part A Molecular & integrative physiology, 2012
    Co-Authors: Yufeng Wang, Kaiqing Rao, Lixia Yuan, Nadia Everaert, Johan Buyse, Roland Grossmann, Ruqian Zhao
    Abstract:

    Fat mass and obesity-associated (FTO) gene is widely expressed in central and peripheral tissues of mammals, and exhibits a range of functions, especially in energy balance. However, basic knowledge of FTO in the chicken is lacking. Therefore, we studied the tissue distribution, age and Breed dependent changes, brain localization, as well as the impact of fasting on FTO mRNA expression in the chicken. FTO mRNA was expressed in all the tissues studied, and generally, with high expression in hypothalamus, liver, visceral fat and cerebellum. However it exhibited Breed-specific patterns: in broilers, the highest expression was seen in the liver, while in layers, hypothalamus and cerebellum showed relatively higher FTO mRNA expression. One-week-old broilers expressed markedly higher FTO mRNA in liver compared with the layers of the same age (P

  • layer and broiler chicks exhibit similar hypothalamic expression of orexigenic neuropeptides but distinct expression of genes related to energy homeostasis and obesity
    Brain Research, 2009
    Co-Authors: Lixia Yuan, Yufeng Wang, Roland Grossmann, Stephan W Barth, Ruqian Zhao
    Abstract:

    Abstract Layer and broiler chickens demonstrate striking Differences in body weight and body composition. However, the mechanism underlying such Difference is elusive. Hypothalamus–pituitary–adrenal (HPA) axis regulates energy homeostasis and body size in mammals, but information in birds is scarce. Here we test the hypothesis that such Breed Difference is more associated with hypothalamic expression of genes related to HPA axis, rather than orexigenic neuropeptides. Broiler chicks exhibit significantly higher body weight and food intake at day (D) 7 posthatching, but the food intake relative to body weight gain was actually lower. No Breed Differences were observed for hypothalamic expression of neuropeptide Y (NPY), agouti-related protein (AGRP), proopiomelanocortin (POMC), orexin (ORX), leptin receptor (LEPR), acetyl-CoA carboxylase (ACC) or fatty acid synthase (FAS). However, broiler chicks expressed significantly higher glucocorticoid receptor (GR) mRNA (P

  • characterization of adrenal acth signaling pathway and steroidogenic enzymes in erhualian and pietrain pigs with different plasma cortisol levels
    Steroids, 2008
    Co-Authors: Dong Xia, Shi Wei, J Hartung, Ruqian Zhao
    Abstract:

    Abstract Our previous study demonstrated significant Difference in the basal plasma cortisol levels between Erhualian (EHL) and Pietrain (PIE) pigs, implicating fundamental Breed Difference in adrenocortical function. The objectives of the present study were therefore to characterize the expression pattern of proteins involved in adrenal ACTH signaling and, including melanocortin type 2 receptor (MC2R), cAMP response element binding protein (CREB) and phosphorylated CREB (pCREB), steroidogenic acute regulatory protein (StAR), as well as that of the key enzymes involved in steroidogenesis in EHL and PIE pigs, in association with the plasma corticotrophin (ACTH) and cortisol levels. The plasma concentrations of the substrates for adrenal steroidogenesis, cholesterol and low-density lipoprotein (LDL) cholesterol, did not differ between Breeds. Plasma concentration of ACTH and the adrenal contents of MC2R mRNA and protein were similar in two Breeds of pigs, whereas the basal plasma concentrations of cortisol in EHL pigs were 1.5 folds higher than that in PIE pigs. The higher basal plasma cortisol levels in EHL pigs were found to be accompanied with the higher expression of ACTH post-receptor signaling components, cAMP, pCREB and StAR, as well as the higher expression of cholesterol side-chain cleavage cytochrome P450 (P450scc), 17α-hydroxylase cytochrome P450 (P45017α), 21-hydroxylase cytochrome P450 (P450c21) and 11β-hydroxylase cytochrome P450 (P45011β). These results indicated that the enhanced cAMP/PKA/pCREB-signaling system and augmented expression of StAR and steroidogenic enzymes are major attributes to the higher basal plasma cortisol concentrations in pigs.

Yanan Yuan - One of the best experts on this subject based on the ideXlab platform.

  • SNP variation in ADRB3 gene reflects the Breed Difference of sheep populations.
    Molecular Biology Reports, 2012
    Co-Authors: Jianliang Wu, Liying Qiao, Yanan Yuan
    Abstract:

    The β3-adrenergic receptor (ADRB3), a G-protein coupled receptor, plays a major role in energy metabolism and regulation of lipolysis and homeostasis. We detect the single nucleotide polymorphism (SNP) variation in full-length sequence of ovine ADRB3 gene in 12 domestic sheep populations within four types by polymerase chain reaction–single strand conformation polymorphism and sequencing to reveal the Breed Difference. Twenty-two SNPs, 12 of which in the exon 1 and ten in the intron, were detected, and 12 new exonic and four new intronic SNPs were found. Most SNPs presented in Shanxi Dam Line and least ones in Dorset. The average SNP number in both meat and dual purpose for meat and wool Breeds was significantly higher than general and dual purpose Breeds for wool and meat. Frequency of each SNP in studied Breeds or types was different. The 18C Del and 1617T Ins majorly existed in dual purpose Breeds for wool and meat. The 25A Del, 119C>G and 130C>T were mostly found in the meat and dual purpose for meat and wool Breeds. The 1764C>A more frequently presented in meat than in other types. The majority of variations came from within the populations as suggested by analysis of molecular variance. Close relationship presented among the Chinese and western Breeds, respectively. In conclusion, SNPs of ovine ADRB3 gene can reflect the Breed Difference and within- and between-population variations, and to a great extent, the Breed relationship.

Y. Reng - One of the best experts on this subject based on the ideXlab platform.

  • Breed Difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα
    Animal Genetics, 2009
    Co-Authors: Tizhong Shan, T. Wu, Y. Reng, Yizhen Wang
    Abstract:

    : Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are major novel triglyceride lipases in animals. The aim of this study was to determine if there are Differences in the porcine ATGL (pATGL) and HSL genes between Jinhua pigs (a fatty Breed) and Landrace pigs (a leaner Breed). In addition, the effect of TNFalpha and pATGL-specific siRNA (pATGL-siRNA) on the expression of pATGL and HSL in porcine adipocytes was also examined. Compared with Landrace pigs, the body weight (BW) of Jinhua pigs was lower (P < 0.01), while intramuscular fat content (in the longissimus dorsi muscle), as well as the back fat thickness and body fat content were higher (P < 0.01). The expression of pATGL and HSL mRNA in Jinhua pigs was lower (P < 0.01) in subcutaneous adipose tissue, and greater (P < 0.01) in longissimus dorsi muscle compared with Landrace pigs. In vitro treatment of porcine adipocytes with TNFalpha decreased (P < 0.01) the glycerol release and the gene expression of pATGL, HSL and PPARgamma in porcine adipocytes. Furthermore, transfection with pATGL-siRNA significantly decreased (P < 0.01) the expression of pATGL, while it had no effect on the expression of HSL. Treatment with 25 ng/ml TNFalpha in conjunction with pATGL-siRNA significantly decreased (P < 0.01) the expression of pATGL and HSL in cultured porcine adipocytes. These results provide useful information to further the understanding of the function of pATGL and HSL in porcine lipid metabolism, which should be applicable to the regulation of fat deposition and improvement of meat quality.