The Experts below are selected from a list of 279 Experts worldwide ranked by ideXlab platform
Behrouz Akbariadergani - One of the best experts on this subject based on the ideXlab platform.
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molecularly imprinted solid phase extraction for the selective determination of Bromhexine in human serum and urine with high performance liquid chromatography
Talanta, 2009Co-Authors: Mehran Javanbakht, Mohammad Hadi Namjumanesh, Behrouz AkbariaderganiAbstract:Abstract In this work, a novel method is described for the determination of Bromhexine in biological fluids using molecularly imprinted solid-phase extraction as the sample cleanup technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and Bromhexine as the template molecule. The novel imprinted polymer was used as a solid-phase extraction sorbent for the extraction of Bromhexine from human serum and urine. Various parameters affecting the extraction efficiency of the polymer have been evaluated. The optimal conditions for molecularly imprinted solid-phase extraction (MISPE) consisted of conditioning 1 mL methanol and 1 mL of deionized water at neutral pH, loading of 5 mL of the water sample (25 μg L −1 ) at pH 6.0, washing using 2 mL acetonitrile/acetone (1/4, v/v) and elution with 3× 1 mL methanol/acetic acid (10/1, v/v). The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of Bromhexine. Results from the HPLC analyses showed that the calibration curve of Bromhexine using MIP from human serum and urine is linear in the ranges of 0.5–100 and 1.5–100 μg L −1 with good precisions (3.3% and 2.8% for 5.0 μg L −1 ), respectively. The recoveries for serum and urine samples were higher than 92%.
Rajan V. Rele - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous UV-spectrophotometric estimation of Bromhexine hydrochloride and salbutamol sulphate by area under curve method in combined dosage form
2015Co-Authors: Rajan V. ReleAbstract:The objective of the study was to develop a simple, accurate, precise and rapid UV spectrophotometric, area under curve (AUC), method for simultaneous estimation of Bromhexine hydrochloride and salbutamol sulphate from combined dosage form. The validation was carried out by using ICH guidelines for the determination of Bromhexine hydrochloride and salbutamol sulphate by using 0.1N hydrochloric acid as the solvent in pharmaceutical dosage form. The proposed area under curve method involves the measurement of area at selected analytical wavelength ranges and performing the analysis using “Cramer’s rule and Matrix method” .The two analytical wavelengths ranges were used i.e. 240-250 nm and 220-230 nm for estimation of Bromhexine hydrochloride and salbutamol sulphate respectively. The linearity of the proposed method was found in the concentration range of 2- 14 µg /ml (r2= 0.9998) for Bromhexine hydrochloride and salbutamol sulphate (r2=0.9999) respectively. The percentage mean recovery was found to be 100.083% for Bromhexine hydrochloride and 100.111 % for salbutamol sulphate respectively. The method was statistically validated for its linearity, accuracy and precision as per ICH guidelines. Both intra and inter day variation showed less percentage (%) RSD values indicating high grade of precision of this method.
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simultaneous determination of amoxicillin trihydrate and Bromhexine hydrochloride in pharmaceutical dosage by reverse phase high performance liquid chromatography
Der Pharma Chemica, 2013Co-Authors: Rajan V. Rele, Rajesh N MaliAbstract:Rapid and accurate high performance liquid chromatography method is described for simultaneous determination of amoxicillin trihydrate and Bromhexine hydrochloride from the combine formulation i.e. capsules. The separation of two drugs was achieved on a ZORBAX Eclips XDB C18 (150 x 4.6 mm i.d.) 5 μ particle size. The mobile phase consisted of methanol, water and triethylamine in the ratio of 50: 50: 0.1(v/v). The pH was adjusted to 3.0 with orthophosphoric acid. The detection was carried out at wavelength 248 nm. The ZORBAX Eclips XDB column showed the most favorable chromatographic parameters for analysis. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of solution. The linear range for amoxicillin trihydrate and Bromhexine hydrochloride was 625 - 1875 μg / ml and 20 - 60 μg / ml respectively. The method has been successfully used to analyse commercial solid dosage i.e. capsules containing 250 mg of amoxicillin trihydrate and 8 mg. of Bromhexine hydrochloride with good recoveries.
Joshi Pranav Pramod - One of the best experts on this subject based on the ideXlab platform.
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simultaneous estimation of amoxicillin trihydrate and Bromhexine hydrochloride in oral solid dosage forms by spectrophotometric method
2011Co-Authors: Dhoka Madhura Vishal, Gawande Vandana Tukaram, Joshi Pranav PramodAbstract:Two accurate, precise, rapid and economic al methods were developed and validated for the estimation of Amoxicillin Trihydrate and Bromhexine Hydrochloride in Bulk and combined Pharmaceutical Dosage Form . First method is First order Derivative method , wherein wavelengths selected for quantitation were 283 nm, for Amoxicillin Trihydrate and 218.6 nm, for Bromhexine Hydrochloride . Second method is area under curve method wherein two wavelength ranges chosen were 271 - 274nm and 244 - 248nm for Amoxicillin trihydrate and Bromhexine hydrochloride respectiv ely. In both of the methods linearity for detector response was observed in the concentration range of 100 - 300 μg/ml for Amoxicillin Trihydrate and 2 - 10 μg/ml for Bromhexine Hydrochloride . The proposed methods were successfully applied for the simultaneous determination of both drugs in c apsule dosage form . The results of the analysis have been validated statistical ly and by recovery studies.
Salvatore Di Somma - One of the best experts on this subject based on the ideXlab platform.
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Potential new treatment strategies for COVID-19: is there a role for Bromhexine as add-on therapy?
Internal and Emergency Medicine, 2020Co-Authors: Markus Depfenhart, Danielle De Villiers, Gottfried Lemperle, Markus Meyer, Salvatore Di SommaAbstract:Of huge importance now is to provide a fast, cost-effective, safe, and immediately available pharmaceutical solution to curb the rapid global spread of SARS-CoV-2. Recent publications on SARS-CoV-2 have brought attention to the possible benefit of chloroquine in the treatment of patients infected by SARS-CoV-2. Whether chloroquine can treat SARS-CoV-2 alone and also work as a prophylactic is doubtful. An effective prophylactic medication to prevent viral entry has to contain, at least, either a protease inhibitor or a competitive virus ACE2-binding inhibitor. Using Bromhexine at a dosage that selectively inhibits TMPRSS2 and, in so doing, inhibits TMPRSS2-specific viral entry is likely to be effective against SARS-CoV-2. We propose the use of Bromhexine as a prophylactic and treatment. We encourage the scientific community to assess Bromhexine clinically as a prophylactic and curative treatment. If proven to be effective, this would allow a rapid, accessible, and cost-effective application worldwide.
M. E. B. Rao - One of the best experts on this subject based on the ideXlab platform.
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Spectrophotometric method for determination of salbutamol and Bromhexine in combined dosage forms
The Indian pharmacist, 2006Co-Authors: K. Ravi Sankar, V. Venkata Rao, V. Kiran Kumar, K. E. V. Nagoji, M. E. B. RaoAbstract:A simple, precise and accurate visible spectrophotometric method was developed for the simultaneous determination of salbutamol and Bromhexine present in combined pharmaceutical dosage forms. Both the drugs upon reaction with 3-methyl-2-benzo thiazolinone hydrazone (MBTH) hydrochloride in presence of ceric ammonium sulphate (CAS) formed pink coloured species and the colour was found to be stable for more than 1 hour. Salbutamol and Bromhexine have λ max at 460nm and 566nm, respectively. When mixed standard solutions containing salbutamol and Bromhexine were treated with MBTH and Ce (IV), they obeyed linearity in the concentration range of 2-20pg/ml and 4-40pg/ml respectively. When commercial formulations were analyzed, the results were found to be in good agreement with the labeled claim. The results obtained indicate that the proposed method could be successfully applied for the simultaneous determination of the above drugs.
