The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
Priti Krishna - One of the best experts on this subject based on the ideXlab platform.
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effects of 24 epibrassinolide on freezing and thermotolerance of bromegrass Bromus Inermis cell cultures
Physiologia Plantarum, 1995Co-Authors: Ronald W. Wilen, Melanie Sacco, Lawrence V. Gusta, Priti KrishnaAbstract:The effect of 24-epibrassinolide (EBS), a brassinosteroid, on the response of smooth bromegrass (Bromus Inermis Leyss) cell suspension cultures under low and high temperature stress was evaluated. EBS minimally increased freezing tolerance of bromegrass cells by 3-5 ° C, but markedly enhanced cell viability following exposure to high temperature stress. The net effect on hardening was, however, less than that obtained with abscisic acid (ABA). Treatment of cells with EBS increased the accumulation of a subset of ABA-inducible heat-stable proteins, but unlike ABA, EBS did not induce the expression of dehydrin transcripts which hybridized to a barley dehydrin clone. EBS slightly increased the hsp90 transcript levels, while ABA reduced the level of expression. These results confirm that brassinosteroids confer some stress tolerance to plant cells and further suggest that the mechanisms by which these compounds exert anti-stress effects may only in part be similar to that of ABA.
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Effects of 24‐epibrassinolide on freezing and thermotolerance of bromegrass (Bromus Inermis) cell cultures
Physiologia Plantarum, 1995Co-Authors: Ronald W. Wilen, Melanie Sacco, Lawrence V. Gusta, Priti KrishnaAbstract:The effect of 24-epibrassinolide (EBS), a brassinosteroid, on the response of smooth bromegrass (Bromus Inermis Leyss) cell suspension cultures under low and high temperature stress was evaluated. EBS minimally increased freezing tolerance of bromegrass cells by 3-5 ° C, but markedly enhanced cell viability following exposure to high temperature stress. The net effect on hardening was, however, less than that obtained with abscisic acid (ABA). Treatment of cells with EBS increased the accumulation of a subset of ABA-inducible heat-stable proteins, but unlike ABA, EBS did not induce the expression of dehydrin transcripts which hybridized to a barley dehydrin clone. EBS slightly increased the hsp90 transcript levels, while ABA reduced the level of expression. These results confirm that brassinosteroids confer some stress tolerance to plant cells and further suggest that the mechanisms by which these compounds exert anti-stress effects may only in part be similar to that of ABA.
Masaya Ishikawa - One of the best experts on this subject based on the ideXlab platform.
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Transformation of suspension cultures of bromegrass (Bromus Inermis) by Agrobacterium tumefaciens
Plant Cell Tissue and Organ Culture, 2006Co-Authors: Toshihide Nakamura, Masaya IshikawaAbstract:Smooth bromegrass (Bromus Inermis Leyss) is an extremely cold hardy perennial grass and its cell culture is an excellent system for studying mechanisms of cold hardiness induced by low temperature or abscisic acid (ABA). Agrobacterium tumefaciens-mediated transformation of non-embryogenic bromegrass cultures was attempted. Agrobacterium strain EHA105 carrying a binary vector that contained the neomycin phosphotransferase (NPT II), beta-glucuronidase (GUS) and green fluorescent protein (GFP) genes were co-cultivated for 3 days with bromegrass cells at the late exponential or early stationary growth phase (7–9 days after subculture). These conditions gave optimal transformation efficiency. Putative transformants were identified by selection for geneticin resistance and by examining the calluses using fluorescence microscopy. This allows the elimination of escapes and selection of cells that express the target genes. PCR and Southern blot analyses confirmed the integration of the GUS and GFP genes into the genome of transformed bromegrass cell lines. Transformants with various levels of GUS expression were obtained with a high frequency following Agrobacterium-mediated gene transfer and visual selection by GFP. The successful transformation method described allows reverse genetics approaches for analyzing cold hardiness genes isolated from bromegrass cells.
Ronald W. Wilen - One of the best experts on this subject based on the ideXlab platform.
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effects of 24 epibrassinolide on freezing and thermotolerance of bromegrass Bromus Inermis cell cultures
Physiologia Plantarum, 1995Co-Authors: Ronald W. Wilen, Melanie Sacco, Lawrence V. Gusta, Priti KrishnaAbstract:The effect of 24-epibrassinolide (EBS), a brassinosteroid, on the response of smooth bromegrass (Bromus Inermis Leyss) cell suspension cultures under low and high temperature stress was evaluated. EBS minimally increased freezing tolerance of bromegrass cells by 3-5 ° C, but markedly enhanced cell viability following exposure to high temperature stress. The net effect on hardening was, however, less than that obtained with abscisic acid (ABA). Treatment of cells with EBS increased the accumulation of a subset of ABA-inducible heat-stable proteins, but unlike ABA, EBS did not induce the expression of dehydrin transcripts which hybridized to a barley dehydrin clone. EBS slightly increased the hsp90 transcript levels, while ABA reduced the level of expression. These results confirm that brassinosteroids confer some stress tolerance to plant cells and further suggest that the mechanisms by which these compounds exert anti-stress effects may only in part be similar to that of ABA.
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Effects of 24‐epibrassinolide on freezing and thermotolerance of bromegrass (Bromus Inermis) cell cultures
Physiologia Plantarum, 1995Co-Authors: Ronald W. Wilen, Melanie Sacco, Lawrence V. Gusta, Priti KrishnaAbstract:The effect of 24-epibrassinolide (EBS), a brassinosteroid, on the response of smooth bromegrass (Bromus Inermis Leyss) cell suspension cultures under low and high temperature stress was evaluated. EBS minimally increased freezing tolerance of bromegrass cells by 3-5 ° C, but markedly enhanced cell viability following exposure to high temperature stress. The net effect on hardening was, however, less than that obtained with abscisic acid (ABA). Treatment of cells with EBS increased the accumulation of a subset of ABA-inducible heat-stable proteins, but unlike ABA, EBS did not induce the expression of dehydrin transcripts which hybridized to a barley dehydrin clone. EBS slightly increased the hsp90 transcript levels, while ABA reduced the level of expression. These results confirm that brassinosteroids confer some stress tolerance to plant cells and further suggest that the mechanisms by which these compounds exert anti-stress effects may only in part be similar to that of ABA.
Lawrence V. Gusta - One of the best experts on this subject based on the ideXlab platform.
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effects of 24 epibrassinolide on freezing and thermotolerance of bromegrass Bromus Inermis cell cultures
Physiologia Plantarum, 1995Co-Authors: Ronald W. Wilen, Melanie Sacco, Lawrence V. Gusta, Priti KrishnaAbstract:The effect of 24-epibrassinolide (EBS), a brassinosteroid, on the response of smooth bromegrass (Bromus Inermis Leyss) cell suspension cultures under low and high temperature stress was evaluated. EBS minimally increased freezing tolerance of bromegrass cells by 3-5 ° C, but markedly enhanced cell viability following exposure to high temperature stress. The net effect on hardening was, however, less than that obtained with abscisic acid (ABA). Treatment of cells with EBS increased the accumulation of a subset of ABA-inducible heat-stable proteins, but unlike ABA, EBS did not induce the expression of dehydrin transcripts which hybridized to a barley dehydrin clone. EBS slightly increased the hsp90 transcript levels, while ABA reduced the level of expression. These results confirm that brassinosteroids confer some stress tolerance to plant cells and further suggest that the mechanisms by which these compounds exert anti-stress effects may only in part be similar to that of ABA.
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Effects of 24‐epibrassinolide on freezing and thermotolerance of bromegrass (Bromus Inermis) cell cultures
Physiologia Plantarum, 1995Co-Authors: Ronald W. Wilen, Melanie Sacco, Lawrence V. Gusta, Priti KrishnaAbstract:The effect of 24-epibrassinolide (EBS), a brassinosteroid, on the response of smooth bromegrass (Bromus Inermis Leyss) cell suspension cultures under low and high temperature stress was evaluated. EBS minimally increased freezing tolerance of bromegrass cells by 3-5 ° C, but markedly enhanced cell viability following exposure to high temperature stress. The net effect on hardening was, however, less than that obtained with abscisic acid (ABA). Treatment of cells with EBS increased the accumulation of a subset of ABA-inducible heat-stable proteins, but unlike ABA, EBS did not induce the expression of dehydrin transcripts which hybridized to a barley dehydrin clone. EBS slightly increased the hsp90 transcript levels, while ABA reduced the level of expression. These results confirm that brassinosteroids confer some stress tolerance to plant cells and further suggest that the mechanisms by which these compounds exert anti-stress effects may only in part be similar to that of ABA.
Melanie Sacco - One of the best experts on this subject based on the ideXlab platform.
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effects of 24 epibrassinolide on freezing and thermotolerance of bromegrass Bromus Inermis cell cultures
Physiologia Plantarum, 1995Co-Authors: Ronald W. Wilen, Melanie Sacco, Lawrence V. Gusta, Priti KrishnaAbstract:The effect of 24-epibrassinolide (EBS), a brassinosteroid, on the response of smooth bromegrass (Bromus Inermis Leyss) cell suspension cultures under low and high temperature stress was evaluated. EBS minimally increased freezing tolerance of bromegrass cells by 3-5 ° C, but markedly enhanced cell viability following exposure to high temperature stress. The net effect on hardening was, however, less than that obtained with abscisic acid (ABA). Treatment of cells with EBS increased the accumulation of a subset of ABA-inducible heat-stable proteins, but unlike ABA, EBS did not induce the expression of dehydrin transcripts which hybridized to a barley dehydrin clone. EBS slightly increased the hsp90 transcript levels, while ABA reduced the level of expression. These results confirm that brassinosteroids confer some stress tolerance to plant cells and further suggest that the mechanisms by which these compounds exert anti-stress effects may only in part be similar to that of ABA.
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Effects of 24‐epibrassinolide on freezing and thermotolerance of bromegrass (Bromus Inermis) cell cultures
Physiologia Plantarum, 1995Co-Authors: Ronald W. Wilen, Melanie Sacco, Lawrence V. Gusta, Priti KrishnaAbstract:The effect of 24-epibrassinolide (EBS), a brassinosteroid, on the response of smooth bromegrass (Bromus Inermis Leyss) cell suspension cultures under low and high temperature stress was evaluated. EBS minimally increased freezing tolerance of bromegrass cells by 3-5 ° C, but markedly enhanced cell viability following exposure to high temperature stress. The net effect on hardening was, however, less than that obtained with abscisic acid (ABA). Treatment of cells with EBS increased the accumulation of a subset of ABA-inducible heat-stable proteins, but unlike ABA, EBS did not induce the expression of dehydrin transcripts which hybridized to a barley dehydrin clone. EBS slightly increased the hsp90 transcript levels, while ABA reduced the level of expression. These results confirm that brassinosteroids confer some stress tolerance to plant cells and further suggest that the mechanisms by which these compounds exert anti-stress effects may only in part be similar to that of ABA.
