The Experts below are selected from a list of 1215 Experts worldwide ranked by ideXlab platform
Baojie Li - One of the best experts on this subject based on the ideXlab platform.
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the noncanonical bmp signaling pathway plays an important role in club cell regeneration
Stem Cells, 2020Co-Authors: Soma Biswas, Yuji Mishina, Baojie Li, Ping LiAbstract:: The Bronchiole is a major site for the development of several life-threatening disorders including chronic obstructive pulmonary disease and lung adenocarcinomas. The bronchiolar epithelium is composed of club cells and ciliated epithelial cells, with club cells serving as progenitor cells. Presently, the identity of the cells involved in regeneration of bronchiolar epithelium and the underlying mechanisms remain incompletely understood. Here, we show that Prrx1, a homeobox transcription factor, can mark club cells in adult mice during homeostasis and regeneration. We further show that the noncanonical signaling pathway of BMPs, BMPR1A-Tak1-p38MAPK, plays a critical role in club cell regeneration. Ablation of Bmpr1a, Tak1, or Mapk14 (encoding p38α) in Prrx1+ club cells caused minimal effect on bronchiolar epithelium homeostasis, yet it resulted in severe defects in club cell regeneration and Bronchiole repair in adult mice. We further show that this pathway supports proliferation and expansion of the regenerating club cells. Our findings thus identify a marker for club cells and reveal a critical role for the BMP noncanonical pathway in club cell regeneration.
Jeongeun Hwang - One of the best experts on this subject based on the ideXlab platform.
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Jinwon L: Quantifying Morphological Parameters of the Terminal Branching Units in a Mouse Lung by Phase Contrast Synchrotron Radiation Computed Tomography. PLoS One 2013
2016Co-Authors: Jeongeun Hwang, Miju Kim, Seunghwan Kim, Jinwon LeeAbstract:An effective technique of phase contrast synchrotron radiation computed tomography was established for the quantitative analysis of the microstructures in the respiratory zone of a mouse lung. Heitzman’s method was adopted for the whole-lung sample preparation, and Canny’s edge detector was used for locating the air-tissue boundaries. This technique revealed detailed morphology of the respiratory zone components, including terminal Bronchioles and alveolar sacs, with sufficiently high resolution of 1.74 mm isotropic voxel size. The technique enabled visual inspection of the respiratory zone components and comprehension of their relative positions in three dimensions. To check the method’s feasibility for quantitative imaging, morphological parameters such as diameter, surface area and volume were measured and analyzed for sixteen randomly selected terminal branching units, each consisting of a terminal Bronchiole and a pair of succeeding alveolar sacs. The four types of asymmetry ratios concerning alveolar sac mouth diameter, alveolar sac surface area, and alveolar sac volume are measured. This is the first ever finding of the asymmetry ratio for the terminal Bronchioles and alveolar sacs, and it is noteworthy that an appreciable degree of branching asymmetry was observed among the alveolar sacs at the terminal end of the airway tree, despite the number of samples was small yet. The series of efficient techniques developed and confirmed in this study, from sample preparation to quantification, is expected to contribute to a wider and exacte
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Quantifying morphological parameters of the terminal branching units in a mouse lung by phase contrast synchrotron radiation computed tomography.
PLOS ONE, 2013Co-Authors: Jeongeun HwangAbstract:An effective technique of phase contrast synchrotron radiation computed tomography was established for the quantitative analysis of the microstructures in the respiratory zone of a mouse lung. Heitzman’s method was adopted for the whole-lung sample preparation, and Canny’s edge detector was used for locating the air-tissue boundaries. This technique revealed detailed morphology of the respiratory zone components, including terminal Bronchioles and alveolar sacs, with sufficiently high resolution of 1.74 µm isotropic voxel size. The technique enabled visual inspection of the respiratory zone components and comprehension of their relative positions in three dimensions. To check the method’s feasibility for quantitative imaging, morphological parameters such as diameter, surface area and volume were measured and analyzed for sixteen randomly selected terminal branching units, each consisting of a terminal Bronchiole and a pair of succeeding alveolar sacs. The four types of asymmetry ratios concerning alveolar sac mouth diameter, alveolar sac surface area, and alveolar sac volume are measured. This is the first ever finding of the asymmetry ratio for the terminal Bronchioles and alveolar sacs, and it is noteworthy that an appreciable degree of branching asymmetry was observed among the alveolar sacs at the terminal end of the airway tree, despite the number of samples was small yet. The series of efficient techniques developed and confirmed in this study, from sample preparation to quantification, is expected to contribute to a wider and exacter application of phase contrast synchrotron radiation computed tomography to a variety of studies.
Naoya Tanabe - One of the best experts on this subject based on the ideXlab platform.
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analysis of airway pathology in copd using a combination of computed tomography micro computed tomography and histology
European Respiratory Journal, 2018Co-Authors: Stijn E Verleden, Naoya Tanabe, Dragos M Vasilescu, Daisuke Kinose, Miranda Kirby, Harvey O Coxson, Bart M Vanaudenaerde, Yasutaka NakanoAbstract:The small conducting airways are the major site of obstruction in chronic obstructive pulmonary disease (COPD). This study examined small airway pathology using a novel combination of multidetector row computed tomography (MDCT), micro-computed tomography (microCT) and histology. Airway branches visible on specimen MDCT were counted and the dimensions of the third- to fifth-generation airways were computed, while the terminal Bronchioles (designated TB), preterminal Bronchioles (TB-1) and pre-preterminal Bronchioles (TB-2) were examined with microCT and histology in eight explanted lungs with end-stage COPD and seven unused donor lungs that served as controls. On MDCT, COPD lungs showed a decrease in the number of 2–2.5 mm diameter airways and the lumen area of fifth-generation airways, while on microCT there was a reduction in the number of terminal Bronchioles as well as a decrease in the luminal areas, wall volumes and alveolar attachments to the walls of TB, TB-1 and TB-2 Bronchioles. The combination of microCT and histology showed increased B-cell infiltration into the walls of TB-1 and TB-2 Bronchioles, and this change was correlated with a reduced number of alveolar attachments in COPD. Small airways disease extends from 2 mm diameter airways to the terminal Bronchioles in COPD. Destruction of alveolar attachments may be driven by a B-cell-mediated immune response in the preterminal Bronchioles.
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micro computed tomography comparison of preterminal Bronchioles in centrilobular and panlobular emphysema
American Journal of Respiratory and Critical Care Medicine, 2017Co-Authors: Naoya Tanabe, John E Mcdonough, Dragos M Vasilescu, Daisuke Kinose, Masaru Suzuki, Joel D Cooper, Peter D Pare, James C HoggAbstract:Rationale: Very little is known about airways that are too small to be visible on thoracic multidetector computed tomography but larger than the terminal Bronchioles.Objectives: To examine the structure of preterminal Bronchioles located one generation proximal to terminal Bronchioles in centrilobular and panlobular emphysema.Methods: Preterminal Bronchioles were identified by backtracking from the terminal Bronchioles, and their centerlines were established along the entire length of their lumens. Multiple cross-sectional images perpendicular to the centerline were reconstructed to evaluate the bronchiolar wall and lumen, and the alveolar attachments to the outer airway walls in relation to emphysematous destruction in 28 lung samples from six patients with centrilobular emphysema, 20 lung samples from seven patients with panlobular emphysema associated with alpha-1 antitrypsin deficiency, and 47 samples from seven control (donor) lungs.Measurements and Main Results: The preterminal bronchiolar length, w...
Daisuke Kinose - One of the best experts on this subject based on the ideXlab platform.
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analysis of airway pathology in copd using a combination of computed tomography micro computed tomography and histology
European Respiratory Journal, 2018Co-Authors: Stijn E Verleden, Naoya Tanabe, Dragos M Vasilescu, Daisuke Kinose, Miranda Kirby, Harvey O Coxson, Bart M Vanaudenaerde, Yasutaka NakanoAbstract:The small conducting airways are the major site of obstruction in chronic obstructive pulmonary disease (COPD). This study examined small airway pathology using a novel combination of multidetector row computed tomography (MDCT), micro-computed tomography (microCT) and histology. Airway branches visible on specimen MDCT were counted and the dimensions of the third- to fifth-generation airways were computed, while the terminal Bronchioles (designated TB), preterminal Bronchioles (TB-1) and pre-preterminal Bronchioles (TB-2) were examined with microCT and histology in eight explanted lungs with end-stage COPD and seven unused donor lungs that served as controls. On MDCT, COPD lungs showed a decrease in the number of 2–2.5 mm diameter airways and the lumen area of fifth-generation airways, while on microCT there was a reduction in the number of terminal Bronchioles as well as a decrease in the luminal areas, wall volumes and alveolar attachments to the walls of TB, TB-1 and TB-2 Bronchioles. The combination of microCT and histology showed increased B-cell infiltration into the walls of TB-1 and TB-2 Bronchioles, and this change was correlated with a reduced number of alveolar attachments in COPD. Small airways disease extends from 2 mm diameter airways to the terminal Bronchioles in COPD. Destruction of alveolar attachments may be driven by a B-cell-mediated immune response in the preterminal Bronchioles.
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micro computed tomography comparison of preterminal Bronchioles in centrilobular and panlobular emphysema
American Journal of Respiratory and Critical Care Medicine, 2017Co-Authors: Naoya Tanabe, John E Mcdonough, Dragos M Vasilescu, Daisuke Kinose, Masaru Suzuki, Joel D Cooper, Peter D Pare, James C HoggAbstract:Rationale: Very little is known about airways that are too small to be visible on thoracic multidetector computed tomography but larger than the terminal Bronchioles.Objectives: To examine the structure of preterminal Bronchioles located one generation proximal to terminal Bronchioles in centrilobular and panlobular emphysema.Methods: Preterminal Bronchioles were identified by backtracking from the terminal Bronchioles, and their centerlines were established along the entire length of their lumens. Multiple cross-sectional images perpendicular to the centerline were reconstructed to evaluate the bronchiolar wall and lumen, and the alveolar attachments to the outer airway walls in relation to emphysematous destruction in 28 lung samples from six patients with centrilobular emphysema, 20 lung samples from seven patients with panlobular emphysema associated with alpha-1 antitrypsin deficiency, and 47 samples from seven control (donor) lungs.Measurements and Main Results: The preterminal bronchiolar length, w...
Annick Clement - One of the best experts on this subject based on the ideXlab platform.
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surfactant protein c gene sftpc mutation associated lung disease high resolution computed tomography hrct findings and its relation to histological analysis
Pediatric Pulmonology, 2010Co-Authors: M Mechri, Ralph Epaud, Sophie Emond, Aurore Coulomb, Francis Jaubert, A Tarrant, Delphine Feldmann, Florence Flamein, Annick ClementAbstract:Aim of the Study Determine high-resolution tomography (HRCT) scan characteristics in children with SFTPC mutation and correlate them to histological findings. Patients and Methods This retrospective multicenter study included 15 children (7 females and 8 males) with SFTPC mutations. HRCT scans have been performed in all the children and lung biopsies in 8 children. Results From all signs assessed on initial HRCT scans, ground-glass opacities (n = 14, 93%) and lung cysts (n = 6, 40%) were predominant. Interlobular septal thickening (n = 1, 7%), air space consolidation (n = 1, 7%), paraseptal emphysema (n = 2, 13%), and pulmonary nodules (n = 1, 7%) were also found. Histological analysis revealed accumulation of macrophages in the alveolar lumen, type II pneumocyte hyperplasia, and alveolar septal thickening. Dilatation of the respiratory Bronchiole and alveolar duct associated with muscular hyperplasia were also described. Interestingly, lung cysts on HRCT scans were associated with dilatation of terminal bronchioli and alveolar duct in lung biopsies. Conclusion In children with SFTPC mutations, HRCT scan finding was highly correlated to the histological findings and, as such, represent a useful tool to identify patients that may require SFTPC gene sequencing. Pediatr Pulmonol. 2010; 45:1021–1029. © 2010 Wiley-Liss, Inc.
