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A Sahul S Hameed – One of the best experts on this subject based on the ideXlab platform.

  • experimental vertical transmission of macrobrachium rosenbergii nodavirus mrnv and extra small virus xsv from Brooders to progeny in macrobrachium rosenbergii and artemia
    Journal of Fish Diseases, 2007
    Co-Authors: R Sudhakaran, V Ishaq P Ahmed, P Haribabu, S C Mukherjee, Sri J Widada, J R Bonami, A Sahul S Hameed

    Abstract:

    White tail disease (WTD) is a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. Experiments were carried out to determine the possibility of vertical transmission of M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) in M. rosenbergii and Artemia. Prawn broodstock inoculated with MrNV and XSV by oral or immersion challenge survived without any clinical signs of WTD. The Brooders spawned 5–7 days after inoculation and the eggs hatched. The survival rate of larvae gradually decreased, and 100% mortality was observed at the post-larvae (PL) stage. Whitish muscle, the typical sign of WTD, was seen in advanced larval developmental stages. The ovarian tissue and fertilized eggs were found to be positive for MrNV/XSV by reverse transcriptasepolymerase chain reaction (RT-PCR) whereas the larval stages showed positive by RT nested PCR (nRT-PCR). In Artemia, reproductive cysts and nauplii derived from challenged Brooders were normal and survival rates were within the expected range for normal rearing conditions. The reproductive cysts were found to be positive for MrNV/ XSV by RT-PCR whereas the nauplii showed MrNV/XSV-positive by nRT-PCR. The PL of M. rosenbergii fed nauplii derived from challenged Artemia Brooders died at 9 days post-inoculum with clinical signs of WTD.

  • experimental transmission and tissue tropism of macrobrachium rosenbergii nodavirus mrnv and its associated extra small virus xsv
    Diseases of Aquatic Organisms, 2004
    Co-Authors: A Sahul S Hameed, Sri J Widada, K Yoganandhan, J R Bonami

    Abstract:

    White tail disease (WTD) was found to be a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. The causative organisms have been identified as M. rosenbergii nodavirus (MrNV) and its associated extra small virus (XSV). Experimentally transmitted to healthy animals, they caused 100% mortality in post-larvae but failed to cause mortality in adult prawns. The RT-PCR assay revealed the presence of both viruses in moribund post-larvae and in gill tissue, head muscle, stomach, intestine, heart, hemolymph, pleopods, ovaries and tail muscle, but not in eyestalks or the hepatopancreas of experimentally infected adult prawns. The presence of these viruses in ovarian tissue indicates the possibility of vertical transmission. Pleopods have been found to be a suitable organ for detecting these viruses in Brooders using the RT-PCR technique.

J R Bonami – One of the best experts on this subject based on the ideXlab platform.

  • experimental vertical transmission of macrobrachium rosenbergii nodavirus mrnv and extra small virus xsv from Brooders to progeny in macrobrachium rosenbergii and artemia
    Journal of Fish Diseases, 2007
    Co-Authors: R Sudhakaran, V Ishaq P Ahmed, P Haribabu, S C Mukherjee, Sri J Widada, J R Bonami, A Sahul S Hameed

    Abstract:

    White tail disease (WTD) is a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. Experiments were carried out to determine the possibility of vertical transmission of M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) in M. rosenbergii and Artemia. Prawn broodstock inoculated with MrNV and XSV by oral or immersion challenge survived without any clinical signs of WTD. The Brooders spawned 5–7 days after inoculation and the eggs hatched. The survival rate of larvae gradually decreased, and 100% mortality was observed at the post-larvae (PL) stage. Whitish muscle, the typical sign of WTD, was seen in advanced larval developmental stages. The ovarian tissue and fertilized eggs were found to be positive for MrNV/XSV by reverse transcriptasepolymerase chain reaction (RT-PCR) whereas the larval stages showed positive by RT nested PCR (nRT-PCR). In Artemia, reproductive cysts and nauplii derived from challenged Brooders were normal and survival rates were within the expected range for normal rearing conditions. The reproductive cysts were found to be positive for MrNV/ XSV by RT-PCR whereas the nauplii showed MrNV/XSV-positive by nRT-PCR. The PL of M. rosenbergii fed nauplii derived from challenged Artemia Brooders died at 9 days post-inoculum with clinical signs of WTD.

  • experimental transmission and tissue tropism of macrobrachium rosenbergii nodavirus mrnv and its associated extra small virus xsv
    Diseases of Aquatic Organisms, 2004
    Co-Authors: A Sahul S Hameed, Sri J Widada, K Yoganandhan, J R Bonami

    Abstract:

    White tail disease (WTD) was found to be a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. The causative organisms have been identified as M. rosenbergii nodavirus (MrNV) and its associated extra small virus (XSV). Experimentally transmitted to healthy animals, they caused 100% mortality in post-larvae but failed to cause mortality in adult prawns. The RT-PCR assay revealed the presence of both viruses in moribund post-larvae and in gill tissue, head muscle, stomach, intestine, heart, hemolymph, pleopods, ovaries and tail muscle, but not in eyestalks or the hepatopancreas of experimentally infected adult prawns. The presence of these viruses in ovarian tissue indicates the possibility of vertical transmission. Pleopods have been found to be a suitable organ for detecting these viruses in Brooders using the RT-PCR technique.

Bagher Mojazi Amiri – One of the best experts on this subject based on the ideXlab platform.

  • gonadotropin releasing hormone analogue gnrha treatment improves the milt production and sperm motility of endangered caspian brown trout salmo trutta caspius over the course of a spawning season
    Aquaculture Research, 2011
    Co-Authors: Saeed Hajirezaee, Bagher Mojazi Amiri, Mehdi Mehrpoosh, Samane Nazeri, Hamid Niksirat

    Abstract:

    To determine the effect of gonadotropin releasing hormone-analogue (GnRHa) treatment on the milt quality of endangered Caspian brown trout, Salmo trutta caspius, the sperm motility (percentage and duration of motility), sperm production (sperm density, spermatocrit and milt volume) and milt pH were measured for GnRHa-treated (the treatment group) and untreated groups (the control group) during the spawning season. For untreated Brooders, the values of the motility per cent, sperm density and spermatocrit decreased continuously during the spawning season while the milt volume, duration of motility and milt pH showed only a significant decrease at the end of the season. For GnRHa-treated males, these parameters increased 14 days after GnRHa treatment (first milt collection) and then decreased continuously towards the end of the season. In addition, the values of milt and sperm density yielded per treated male were higher than that in the untreated group, although these were not statistically different. In any case, the total sum of yielded milt from the treatment group over the spawning season was higher than that in the untreated group. In this experiment, significant positive correlations were found between milt parameters as follows: sperm motility vs. milt pH; sperm density vs. spermatocrit; milt volume vs. spermatocrit; and milt volume vs. sperm density. The results show that the treatment of Caspian brown trout by GnRHa can improve the milt quality in terms of sperm motility and sperm production during a spawning season.

  • Comparative analysis of milt quality in the cultured and wild stocks of endangered Caspian brown trout, Salmo trutta caspius
    African Journal of Biotechnology, 2011
    Co-Authors: Saeed Hajirezaee, Hojatollah Jafaryan, Masoud Asghari, Amin Golpour, Bagher Mojazi Amiri

    Abstract:

    The sperm motility characteristics (percentage of motile spermatozoa and duration of motility) and sperm production (spermatocrit, milt volume and sperm concentration) were measured in order to compare the milt quality between cultured and wild stocks of Caspian brown trout, Salmo trutta caspius . Our results showed that cultured Brooders produce more dense milt than wild individuals. In contrast, the milt volume, percentage and duration of spermatozoa motility were higher in wild Brooders than in cultured individuals. The aim of the present study was to assess the effect of captivity condition on milt quality of cultured males of Caspian brown trout. Keywords: Sperm density, sperm motility, Caspian brown trout

  • fish milt quality and major factors influencing the milt quality parameters a review
    African Journal of Biotechnology, 2010
    Co-Authors: Saeed Hajirezaee, Bagher Mojazi Amiri, Alireza Mirvaghefi

    Abstract:

    In commercial fish production, the evaluation of milt quality is essential in order to increase the efficiency of artificial fertilization. Numerous studies have demonstrated that qualitative parameters of milt (i.e. seminal fluid composition, spermatozoa motility and sperm production) could be influence by several factors including biological characteristics of Brooders (age, weight and length), rearing conditions of Brooders (temperature, photoperiod, nourishment, undesirable components and animal welfare and health), artificial induction of spawning, spawning season (repeated milt collection and spermiation time) and post stripping factors (chemical properties of diluents and short-term and long-term storage of milt). In the present paper, we review the roles of these factors on quality of milt fish. On the whole understanding of the factors that affect milt quality could be useful for adjustment and efficient management of these factors in order to obtain good milt for fertilization. Keywords: Fish production, milt quality, artificial fertilization