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Nora R. Ceballos - One of the best experts on this subject based on the ideXlab platform.
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mechanism of hcg induced Spermiation in the toad rhinella arenarum amphibia anura
General and Comparative Endocrinology, 2010Co-Authors: Clara M Volonteri, Nora R. CeballosAbstract:In Rhinella arenarum Spermiation occurs as a consequence of LH/FSH increase during the amplexus or by a single dose of hCG, among other gonadotropins. The present study employs an in vitro system to study the mechanism of action of hCG in the Spermiation of R. arenarum. Testicular fragments were incubated for 2h at 28°C in the presence or absence of 20IU hCG with or without different PKA/PKC inhibitors and activators as well as ouabain and amiloride as Na(+)/K(+) ATPase and transcellular Na(+) transport inhibitors, respectively. Ouabain did not induce Spermiation in absence of hCG and inhibited hCG-induced Spermiation in a dose-dependent manner, reaching 90% inhibition with the higher concentration. In contrast, amiloride neither affected Spermiation nor steroidogenesis. Activation of PKA with 8Br-cAMP induced Spermiation in the absence of hCG while its inhibition with H89 blocked hCG action. On the other hand, PKC inhibition with Bi or STP did not affect hCG-induced Spermiation although PKC activation significantly decreased hCG-dependent sperm release. These results suggest that PKC inhibits Spermiation but also that the inhibition exerted by the kinase could be blocked by hCG. Taken together, these observations could indicate that PKA is involved in the mechanism of the gonadotropin action, mechanism also requiring the activation of a non-pumping Na(+)/K(+) ATPase pathway.
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Relationship between steroidogenesis and Spermiation in Rana catesbeiana and Leptodactylus ocellatus.
Journal of Comparative Physiology B, 2006Co-Authors: Cinthia Rosemblit, Andrea G. Pozzi, Nora R. CeballosAbstract:The present study employs an in vitro system to analyse the role of steroid hormones in hCG-induced Spermiation in two species of anuran amphibian: Rana catesbeiana and Leptodactylus ocellatus. In vitro Spermiation was induced with 10 IU hCG and the effect of different steroid-biosynthesis inhibitors was analysed. Cyanoketone (10−5 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of androgen was significantly reduced. These results clearly showed that, in both species, Spermiation-inducing action of hCG does not depend on the biosynthesis of 3-oxo-4-ene steroids. Moreover, when combined inhibitors, aminoglutethimide (10−5 M) plus cyanoketone (10−5 M), were employed, Spermiation evoked by hCG was not modified while hCG-induced androgen secretion significantly decreased. Additionally, none of the steroids used, progesterone, 17, 20α-dihydroxy-4-pregnen-3-one, testosterone and 5α-dihydrotestosterone, were able to induce Spermiation in the absence of hCG, confirming that steroids are not involved in that process. In conclusion, as previously described in Bufo arenarum, in L. ocellatus and R. catesbeiana hCG-induced Spermiation does not depend on steroid biosynthesis.
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effect of human gonadotropins on Spermiation and androgen biosynthesis in the testis of the toad bufo arenarum amphibia anura
Journal of Experimental Zoology Part A: Comparative Experimental Biology, 2006Co-Authors: Andrea G. Pozzi, Cinthia Rosemblit, Nora R. CeballosAbstract:This paper analyzes, in the toad Bufo arenarum, the effect on Spermiation and androgen secretion of two human recombinant gonadotropins, human recombinant LH (hrLH) and human recombinant FSH (hrFSH) as well as the well-known Spermiation-inducing hormone, human chorionic gonadotropin (hCG). For this purpose, testes were incubated with different concentrations of hrLH (0.01–2.5 µg/ml) and hrFSH (0.05–5 µg/ml), and results were compared with those obtained with 2.5 µg/ml hCG. Spermiation was most efficiently stimulated by hrFSH, which elicited a higher response than either hrLH or hCG. Both hrFSH and hrLH produced a bell-shaped dose–response curve, with a 50% inhibition on Spermiation at a concentration twice higher than that necessary to get the highest response. However, none of the gonadotropins yielded a biphasic response on androgen secretion, hrLH producing the highest response at a concentration that evoked a 70% inhibition in the Spermiation test. Regarding steroidogenesis, hrLH and hrFSH were more active than hCG. Taken together, the results described in this paper suggest that, in B. arenarum, Spermiation and androgen secretion are mediated by different receptors. After comparing the effects of recombinant hormones, we conclude that hrFSH has a greater effect on Spermiation than hCG or hrLH. J. Exp. Zool. 305A:96–102, 2006. © 2005 Wiley-Liss, Inc.
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human chorionic gonadotropin induced Spermiation in bufo arenarum is not mediated by steroid biosynthesis
General and Comparative Endocrinology, 2000Co-Authors: Andrea G. Pozzi, Nora R. CeballosAbstract:This study employed an in vitro system to identify potential steroidal mediators of Spermiation in Bufo arenarum. Testicular fragments were incubated for 2 h at 28 degrees. Spermiation was induced by 10 IU human chorionic gonadotropin (hCG) and the effect of different inhibitors of steroid biosynthesis was analyzed. Cyanoketone (10(-5)-10(-6) M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of 3-oxo-4-ene steroids and its reduced metabolites was inhibited by 95%. Aminogluthetimide at a concentration that inhibited testosterone biosynthesis (10(-4) and 10(-5) M) did not alter hCG actions. Similar results were obtained with spironolactone, an inhibitor of 17alpha-hydroxylase/17-20 lyase activity. No Spermiation-inducing activity was found with different steroids (progesterone, 17-hydroxypregnenolone, 17, 20alpha/beta-dihydroxy-4-pregnene-3-one, estradiol, testosterone, etc.). It is concluded that Spermiation induced by hCG is not steroid mediated in B. arenarum.
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Human chorionic gonadotropin-induced Spermiation in Bufo arenarum is not mediated by steroid biosynthesis.
General and Comparative Endocrinology, 2000Co-Authors: Andrea G. Pozzi, Nora R. CeballosAbstract:This study employed an in vitro system to identify potential steroidal mediators of Spermiation in Bufo arenarum. Testicular fragments were incubated for 2 h at 28°. Spermiation was induced by 10 IU human chorionic gonadotropin (hCG) and the effect of different inhibitors of steroid biosynthesis was analyzed. Cyanoketone (10−5–10−6 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of 3-oxo-4-ene steroids and its reduced metabolites was inhibited by 95%. Aminogluthetimide at a concentration that inhibited testosterone biosynthesis (10−4 and 10−5 M) did not alter hCG actions. Similar results were obtained with spironolactone, an inhibitor of 17α-hydroxylase/17-20 lyase activity. No Spermiation-inducing activity was found with different steroids (progesterone, 17-hydroxypregnenolone, 17,20α/β-dihydroxy-4-pregnene-3-one, estradiol, testosterone, etc.). It is concluded that Spermiation induced by hCG is not steroid mediated in B. arenarum.
Andrea G. Pozzi - One of the best experts on this subject based on the ideXlab platform.
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Relationship between steroidogenesis and Spermiation in Rana catesbeiana and Leptodactylus ocellatus.
Journal of Comparative Physiology B, 2006Co-Authors: Cinthia Rosemblit, Andrea G. Pozzi, Nora R. CeballosAbstract:The present study employs an in vitro system to analyse the role of steroid hormones in hCG-induced Spermiation in two species of anuran amphibian: Rana catesbeiana and Leptodactylus ocellatus. In vitro Spermiation was induced with 10 IU hCG and the effect of different steroid-biosynthesis inhibitors was analysed. Cyanoketone (10−5 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of androgen was significantly reduced. These results clearly showed that, in both species, Spermiation-inducing action of hCG does not depend on the biosynthesis of 3-oxo-4-ene steroids. Moreover, when combined inhibitors, aminoglutethimide (10−5 M) plus cyanoketone (10−5 M), were employed, Spermiation evoked by hCG was not modified while hCG-induced androgen secretion significantly decreased. Additionally, none of the steroids used, progesterone, 17, 20α-dihydroxy-4-pregnen-3-one, testosterone and 5α-dihydrotestosterone, were able to induce Spermiation in the absence of hCG, confirming that steroids are not involved in that process. In conclusion, as previously described in Bufo arenarum, in L. ocellatus and R. catesbeiana hCG-induced Spermiation does not depend on steroid biosynthesis.
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effect of human gonadotropins on Spermiation and androgen biosynthesis in the testis of the toad bufo arenarum amphibia anura
Journal of Experimental Zoology Part A: Comparative Experimental Biology, 2006Co-Authors: Andrea G. Pozzi, Cinthia Rosemblit, Nora R. CeballosAbstract:This paper analyzes, in the toad Bufo arenarum, the effect on Spermiation and androgen secretion of two human recombinant gonadotropins, human recombinant LH (hrLH) and human recombinant FSH (hrFSH) as well as the well-known Spermiation-inducing hormone, human chorionic gonadotropin (hCG). For this purpose, testes were incubated with different concentrations of hrLH (0.01–2.5 µg/ml) and hrFSH (0.05–5 µg/ml), and results were compared with those obtained with 2.5 µg/ml hCG. Spermiation was most efficiently stimulated by hrFSH, which elicited a higher response than either hrLH or hCG. Both hrFSH and hrLH produced a bell-shaped dose–response curve, with a 50% inhibition on Spermiation at a concentration twice higher than that necessary to get the highest response. However, none of the gonadotropins yielded a biphasic response on androgen secretion, hrLH producing the highest response at a concentration that evoked a 70% inhibition in the Spermiation test. Regarding steroidogenesis, hrLH and hrFSH were more active than hCG. Taken together, the results described in this paper suggest that, in B. arenarum, Spermiation and androgen secretion are mediated by different receptors. After comparing the effects of recombinant hormones, we conclude that hrFSH has a greater effect on Spermiation than hCG or hrLH. J. Exp. Zool. 305A:96–102, 2006. © 2005 Wiley-Liss, Inc.
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human chorionic gonadotropin induced Spermiation in bufo arenarum is not mediated by steroid biosynthesis
General and Comparative Endocrinology, 2000Co-Authors: Andrea G. Pozzi, Nora R. CeballosAbstract:This study employed an in vitro system to identify potential steroidal mediators of Spermiation in Bufo arenarum. Testicular fragments were incubated for 2 h at 28 degrees. Spermiation was induced by 10 IU human chorionic gonadotropin (hCG) and the effect of different inhibitors of steroid biosynthesis was analyzed. Cyanoketone (10(-5)-10(-6) M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of 3-oxo-4-ene steroids and its reduced metabolites was inhibited by 95%. Aminogluthetimide at a concentration that inhibited testosterone biosynthesis (10(-4) and 10(-5) M) did not alter hCG actions. Similar results were obtained with spironolactone, an inhibitor of 17alpha-hydroxylase/17-20 lyase activity. No Spermiation-inducing activity was found with different steroids (progesterone, 17-hydroxypregnenolone, 17, 20alpha/beta-dihydroxy-4-pregnene-3-one, estradiol, testosterone, etc.). It is concluded that Spermiation induced by hCG is not steroid mediated in B. arenarum.
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Human chorionic gonadotropin-induced Spermiation in Bufo arenarum is not mediated by steroid biosynthesis.
General and Comparative Endocrinology, 2000Co-Authors: Andrea G. Pozzi, Nora R. CeballosAbstract:This study employed an in vitro system to identify potential steroidal mediators of Spermiation in Bufo arenarum. Testicular fragments were incubated for 2 h at 28°. Spermiation was induced by 10 IU human chorionic gonadotropin (hCG) and the effect of different inhibitors of steroid biosynthesis was analyzed. Cyanoketone (10−5–10−6 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of 3-oxo-4-ene steroids and its reduced metabolites was inhibited by 95%. Aminogluthetimide at a concentration that inhibited testosterone biosynthesis (10−4 and 10−5 M) did not alter hCG actions. Similar results were obtained with spironolactone, an inhibitor of 17α-hydroxylase/17-20 lyase activity. No Spermiation-inducing activity was found with different steroids (progesterone, 17-hydroxypregnenolone, 17,20α/β-dihydroxy-4-pregnene-3-one, estradiol, testosterone, etc.). It is concluded that Spermiation induced by hCG is not steroid mediated in B. arenarum.
Liza Odonnell - One of the best experts on this subject based on the ideXlab platform.
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mechanisms of spermiogenesis and Spermiation and how they are disturbed
Spermatogenesis, 2014Co-Authors: Liza OdonnellAbstract:Haploid round spermatids undergo a remarkable transformation during spermiogenesis. The nucleus polarizes to one side of the cell as the nucleus condenses and elongates, and the microtubule-based manchette sculpts the nucleus into its species-specific head shape. The assembly of the central component of the sperm flagellum, known as the axoneme, begins early in spermiogenesis, and is followed by the assembly of secondary structures needed for normal flagella. The final remodelling of the mature elongated spermatid occurs during Spermiation, when the spermatids line up along the luminal edge, shed their residual cytoplasm and are ultimately released into the lumen. Defects in spermiogenesis and Spermiation are manifested as low sperm number, abnormal sperm morphology and poor motility and are commonly observed during reproductive toxicant administration, as well as in genetically modified mouse models of male infertility. This chapter summarizes the major physiological processes and the most commonly observed defects in spermiogenesis and Spermiation, to aid in the diagnosis of the potential mechanisms that could be perturbed by experimental manipulation such as reproductive toxicant administration.
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Spermiation the process of sperm release
Spermatogenesis [P], 2011Co-Authors: Liza Odonnell, Peter K. Nicholls, Robert I Mclachlan, Moira K Obryan, Peter G StantonAbstract:Spermiation is the process by which mature spermatids are released from Sertoli cells into the seminiferous tubule lumen prior to their passage to the epididymis. It takes place over several days at the apical edge of the seminiferous epithelium, and involves several discrete steps including remodelling of the spermatid head and cytoplasm, removal of specialized adhesion structures and the final disengagement of the spermatid from the Sertoli cell. Spermiation is accomplished by the co-ordinated interactions of various structures, cellular processes and adhesion complexes which make up the “Spermiation machinery”. This review addresses the morphological, ultrastructural and functional aspects of mammalian Spermiation. The molecular composition of the Spermiation machinery, its dynamic changes and regulatory factors are examined. The causes of Spermiation failure and their impact on sperm morphology and function are assessed in an effort to understand how this process may contribute to sperm count suppress...
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a complex containing alpha6beta1 integrin and phosphorylated focal adhesion kinase between sertoli cells and elongated spermatids during spermatid release from the seminiferous epithelium
Journal of Endocrinology, 2006Co-Authors: Amanda Beardsley, David Robertson, Liza OdonnellAbstract:Spermiation is the final step of spermatogenesis and culminates in the disengagement (release) of elongated spermatids from Sertoli cells into the seminiferous tubule lumen. Spermiation failure, wherein spermatids are retained by Sertoli cells instead of releasing, occurs after hormone suppression. The mechanisms involved in spermatid disengagement and retention are not well understood. We previously showed that b1-integrin is associated with spermatids until the point of disengagement, but the ectoplasmic specialisation junction (ES) is not. The aims of this paper are to further characterise the complex that is present immediately prior to spermatid disengagement by identifying the a-integrin form dimerised with b1-integrin, localising focal adhesion kinase (FAK) and determining if microtubules are involved. Adult Sprague‐Dawley rats received testosterone and oestradiol implants and an FSH antibody for 7 days to suppress testicular testosterone and FSH and induce Spermiation failure. Control rats were treated with saline. Immunohistochemical analysis showed that a6-integrin and a phosphorylated form of FAK (FAK-Tyr 397 ) are present between late spermatids and Sertoli cells after ES removal, until the point of disengagement, and both proteins remain associated with retained spermatids after Spermiation failure induced by hormone suppression. Using dual-label immunofluorescence, tubulins (and thus microtubules) were observed to co-localise with ES, but were neither associated with elongated spermatids just prior to release nor with retained spermatids following hormone suppression. These results suggest that microtubules are not involved in the final release of spermatids from Sertoli cells. We conclude that spermatid release during Spermiation is mediated by a ‘disengagement complex’ containing a6b1-integrin and phospho-FAK, the function of which can be affected by gonadotrophin suppression.
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127 an α6β1 integrin focal adhesion kinase complex may regulate Spermiation and Spermiation failure
Reproduction Fertility and Development, 2004Co-Authors: A J Beardsley, David Robertson, Liza OdonnellAbstract:Spermiation is the final step of spermatogenesis (sperm production) where mature spermatids are released from the somatic Sertoli cells. Spermiation is hormone sensitive; testosterone (T) and FSH withdrawal causes a disruption to the disengagement of spermatids, which are instead retained by Sertoli cells. The mechanisms involved with spermatid release and retention are not understood. We showed previously that an unknown adhesion junction containing β1-integrin persisted on retained spermatids suggesting that a defect in this adhesion complex at disengagement may underlie Spermiation failure. The aim of this study is to identify the α-integrin dimerised with β1-integrin and investigate the role of phosphorylated FAK, a kinase that is involved with integrin-mediated cell adhesion, during Spermiation and Spermiation failure. Four adult Sprague-Dawley rats received T and oestradiol implants and FSH antibody for 7A days to suppress testicular T and FSH and induce Spermiation failure. Using immunohistochemistry, α6-integrin (but not α4-integrin) and FAK-Tyr397 were localised on the Sertoli cell plasma membrane adjacent to mature spermatids. This localisation was observed until the point of spermatid release and remained on the Sertoli cell that surrounded retained spermatids after hormone suppression. A similar localisation has been previously observed with β1-integrin, suggesting that all three form a complex at the site of disengagement. To look at the function of FAK-Tyr397, comparative Western blot analysis is currently being undertaken on seminiferous tubules specific for Spermiation from control and treated animals. Preliminary studies suggest that FAK-Tyr397 remains phosphorylated during Spermiation failure, suggesting that FAK dephosphorylation may be important for the function of spermatid-associated adhesion complexes, as has been demonstrated in other adhesion systems. In conclusion, α6β1-integrin/FAK-containing adhesion complexes are associated with spermatids during Spermiation, and the function of such complexes are likely to be perturbed during Spermiation failure.
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characterization of normal Spermiation and Spermiation failure induced by hormone suppression in adult rats
Biology of Reproduction, 2003Co-Authors: Amanda Beardsley, Liza OdonnellAbstract:At the end of spermatogenesis, elongated spermatids are released from supporting Sertoli cells via the process termed Spermiation. Previous studies have shown that Spermiation failure occurs after hormone suppression, in which spermatids are retained instead of releasing. However, the molecular mechanisms involved in Spermiation and Spermiation failure are largely unknown. The aims of the present study were, first, to characterize the ultrastructural events associated with normal Spermiation and Spermiation failure using light and electron microscopy and, second, to investigate the localization of cell adhesion-associated (b1-integrin and cadherins) and junction-associated molecules (integrin-associated kinase [ILK], b-catenin, and espin) during these processes. Four adult Sprague-Dawley rats received testosterone and estradiol implants and FSH antibody (2 mg kg 21 day 21 ) for 7 days to suppress testicular testosterone and FSH and to induce Spermiation failure. Four rats treated with saline were used as controls. After testosterone and FSH suppression, Spermiation at the ultrastructural level appeared to be normal until the final disengagement of the spermatids from Sertoli cells (stage VIII), at which stage a large number of retained spermatids were noted. Immunohistochemical localization of espin showed that during Spermiation, removal of the ectoplasmic specialization (ES) occurred 30 h before spermatid disengagement, suggesting that non-ES junctions mediate the spermatidSertoli cell interaction before and during disengagement. b1-Integrin and b-catenin remained associated with spermatids after ES removal and until disengagement; however, ILK was removed along with the ES. Though detectable, N-cadherin was not associated with the spermatid-Sertoli cell junction. After testosterone and FSH suppression, b1-integrin, but not N-cadherin or b-catenin, remained associated with spermatids that failed to spermiate. In conclusion, hormone suppression-induced Spermiation failure is caused by defects in the disengagement of spermatids from the Sertoli cell, and this process likely is mediated by b1-integrin in an ILK-independent mechanism. follicle-stimulating hormone, Sertoli cells, spermatid, spermatogenesis, testosterone
Sylvain Milla - One of the best experts on this subject based on the ideXlab platform.
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plasma 11 deoxycorticosterone doc and mineralocorticoid receptor testicular expression during rainbow trout oncorhynchus mykiss Spermiation implication with 17alpha 20beta dihydroxyprogesterone on the milt fluidity
Reproductive Biology and Endocrinology, 2008Co-Authors: Sylvain Milla, Xavier Terrien, Armin Sturm, Fidaa Ibrahim, Franck Giton, J Fiet, Patrick PrunetAbstract:Background In rainbow trout (Oncorhynchus mykiss), the endocrine control of Spermiation is not fully understood. Besides 11ketotestosterone (11KT) and 17alpha, 20beta-dihydroxyprogesterone (MIS), the potential physiological ligand of the mineralocorticoid receptor (MR) 11-deoxycorticosterone (DOC), is a credible candidate in O. mykiss Spermiation regulation as Spermiation is accompanied with changes in aqueous and ionic flows.
J F Asturiano - One of the best experts on this subject based on the ideXlab platform.
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using specific recombinant gonadotropins to induce spermatogenesis and Spermiation in the european eel anguilla anguilla
Theriogenology, 2018Co-Authors: D S Penaranda, V Gallego, L Perez, C Rozenfeld, J G Herranzjusdado, Ana M Gomez, Ignacio Gimenez, J F AsturianoAbstract:New specific European eel (Anguilla anguilla) recombinant gonadotropins (aarGths) produced in the ovarian cells of Chinese hamsters (CHO) were used to induce maturation in captive male eels. In the first experiment, five different hormonal treatments were assayed: one group was given a constant dose of recombinant European eel follicle-stimulating hormone (aarFsh; 4 μg/fish) for 9 weeks, and the second group received a constant dose of recombinant European eel luteinizing hormone (aarLh; 2 μg/fish) also for 9 weeks. The other three groups were injected with different combinations of both aarGths (some doses constant, some variable). All five treatments stimulated androgen synthesis, but the increase was more pronounced in the fish treated with a combination of both aarGths. Unlike aarLh, aarFsh alone was able to induce Spermiation, the best results were achieved in the fish that were treated with a constant dose of aarFSH and an increasing dose of aarLH, with Spermiation being induced (20% motile cells) despite the fact that these fish were immature at the start of the experiment. In order to improve sperm quality, a second experiment was performed. Immature males received three constant doses of aarFsh (2.8, 1.4 or 0.7 μg/fish) and increasing doses of aarLh (every 3 weeks; 1, 2, 6 μg/fish). All the treatments induced Spermiation, however the best sperm quality (with ≥50% motile cells) was observed in the males treated with the highest dose of aarFsh. In conclusion, these specific recombinant gonadotropins have demonstrated their capacity to induce spermatogenesis and Spermiation in vivo in a teleost fish, the European eel.
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study of the effects of thermal regime and alternative hormonal treatments on the reproductive performance of european eel males anguilla anguilla during induced sexual maturation
Aquaculture, 2012Co-Authors: V Gallego, I Mazzeo, M C Vilchez, D S Penaranda, P C F Carneiro, L Perez, J F AsturianoAbstract:Abstract Since 1960, the European eel (Anguilla anguilla) has suffered a dramatic reduction in natural stocks. Breeding in captivity is considered an alternative, but obtaining high quality sperm seems basic on this regard. The main objective of this study was to assess the effects of three thermal regimes (two of them variable: T10 and T15; and one of them constant: T20) and three hormonal treatments with different hormones (hCG, hCGrec and PSMG) on the induction of maturation in European eel males. In the case of the thermal regimes, our results demonstrated that the onset and progression of Spermiation are strongly influenced, and perhaps closely regulated, by water temperature. T20 demonstrated the best results in all the sperm parameters (volume, density, motility, kinetic features, etc.) throughout most weeks of treatment, becoming a reliable and productive method for inducing Spermiation in this species. In the case of hormonal treatments, the onset and progression of Spermiation in European eel males were influenced by the hormone used. In this respect, hCGrec produced the best results in all the sperm parameters including volume, density, motility, kinetic features, etc., throughout most weeks of treatment, thus becoming an effective alternative treatment to the standard hCG treatment used to induce Spermiation in eel species. Moreover, hCGrec gave rise to the best economical profitability, making it possible to obtain good quality sperm samples at a lower price than by using the other two hormonal treatments.
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effect of different methods for the induction of Spermiation on semen quality in european eel
Aquaculture Research, 2005Co-Authors: J F Asturiano, D S Penaranda, L Perez, Daniel L Garzon, F Marcojimenez, Silvia Martinezllorens, Ana Tomas, M JoverAbstract:Five hormonal treatments with human chorionic gonadotropin (hCG) were tested for the induction of maturation and Spermiation in male farmed eels. The main aim was to optimize previously used hormonal treatments to achieve shorter induction treatments, longer Spermiation periods and/or higher sperm quality. Fish treated for just 3 weeks (treatment E) or until the onset of Spermiation (treatment C) showed the worst results, while the treatment consisting of weekly administration of 1.5 IU hCG g−1 fish (treatment A) induced the highest percentage of spermiating males, the highest number of sperm samples and sperm volumes and densities similar to the rest of the treatments (B: half hormone dosage, or D: biweekly administration). Evaluation of the sperm quality was performed by computer-assisted sperm analysis (CASA), considering the percentage of total motile spermatozoa, the percentage of fast and medium-velocity spermatozoa, as well as different motility parameters. Sperm samples from A-D groups showed between 44% and 54% motile spermatozoa, and between 10% and 15% fast spermatozoa, while samples from E-treated males showed 0% motile cells. No significant differences were found in the spermatozoa straight line velocity (VSL), curvilinear velocity (VCL) or the angular velocity (VAP), neither spermatozoa beating cross frequency (BCF) between A–D groups.
