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Jongsheek Kim - One of the best experts on this subject based on the ideXlab platform.
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a taqman real time pcr assay for quantifying white spot syndrome virus wssv infections in wild Broodstock and hatchery reared postlarvae of fleshy shrimp fenneropenaeus chinensis
Aquaculture, 2009Co-Authors: Inkwon Jang, Xianhong Meng, Hyungchul Seo, Yeongrok Cho, Bongrae Kim, Gopalakannan Ayyaru, Jongsheek KimAbstract:Abstract In the present study, a highly sensitive and specific TaqMan real-time PCR was developed to quantify white spot syndrome virus (WSSV) infections in wild Broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis . A total of 159 individuals of wild F. chinensis brooders from 3 locations were captured and 210 postlarvae (PL 1–8 ) were obtained from seven commercial hatcheries in 2007 in South Korea. The WSSV infections in 3 Broodstocks showed a wide range, from 0 to 2.28 × 10 6 (with a mean of 1.50 × 10 4 ) copies ng − 1 of DNA. Out of 159 brooders assayed, 39 (24.5%) were negative and 120 (75.5%) were positive; 153 (96.2%) showed less than 100 copies (mean 10.2 copies), 111 (69.8%) showed less than 10 copies and only 6 individuals (3.8%) showed high infections with a range of 2.36 × 10 2 to 2.28 × 10 6 copies ng − 1 of DNA. In 210 postlarvae, a range of 2.6 to 713.6 (with a mean of 220) copies g − 1 of DNA was observed. The mean WSSV copy number in the postlarvae was 7.9 × 10 5 , which was equivalent to 8.5 × 10 5 copies mg − 1 of postlarvae weight. A total of 87.1% of postlarvae were WSSV positive and except for two hatcheries (H 4 and H 7 ), the postlarvae of all the other five hatcheries were positive. Even postlarvae from the same hatchery, especially of hatcheries H 4 and H 5 , showed a wide range of WSSV infection resulting in higher infections than other hatcheries. There were 34.3% of the postlarvae assayed in the present study that showed very low infection, with less than 10 copies ng − 1 of DNA. Based on our results, it is recommended to pre-screen Broodstock or larvae for selective breeding, stocking in production systems or stock enhancement.
Inkwon Jang - One of the best experts on this subject based on the ideXlab platform.
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a taqman real time pcr assay for quantifying white spot syndrome virus wssv infections in wild Broodstock and hatchery reared postlarvae of fleshy shrimp fenneropenaeus chinensis
Aquaculture, 2009Co-Authors: Inkwon Jang, Xianhong Meng, Hyungchul Seo, Yeongrok Cho, Bongrae Kim, Gopalakannan Ayyaru, Jongsheek KimAbstract:Abstract In the present study, a highly sensitive and specific TaqMan real-time PCR was developed to quantify white spot syndrome virus (WSSV) infections in wild Broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis . A total of 159 individuals of wild F. chinensis brooders from 3 locations were captured and 210 postlarvae (PL 1–8 ) were obtained from seven commercial hatcheries in 2007 in South Korea. The WSSV infections in 3 Broodstocks showed a wide range, from 0 to 2.28 × 10 6 (with a mean of 1.50 × 10 4 ) copies ng − 1 of DNA. Out of 159 brooders assayed, 39 (24.5%) were negative and 120 (75.5%) were positive; 153 (96.2%) showed less than 100 copies (mean 10.2 copies), 111 (69.8%) showed less than 10 copies and only 6 individuals (3.8%) showed high infections with a range of 2.36 × 10 2 to 2.28 × 10 6 copies ng − 1 of DNA. In 210 postlarvae, a range of 2.6 to 713.6 (with a mean of 220) copies g − 1 of DNA was observed. The mean WSSV copy number in the postlarvae was 7.9 × 10 5 , which was equivalent to 8.5 × 10 5 copies mg − 1 of postlarvae weight. A total of 87.1% of postlarvae were WSSV positive and except for two hatcheries (H 4 and H 7 ), the postlarvae of all the other five hatcheries were positive. Even postlarvae from the same hatchery, especially of hatcheries H 4 and H 5 , showed a wide range of WSSV infection resulting in higher infections than other hatcheries. There were 34.3% of the postlarvae assayed in the present study that showed very low infection, with less than 10 copies ng − 1 of DNA. Based on our results, it is recommended to pre-screen Broodstock or larvae for selective breeding, stocking in production systems or stock enhancement.
Marisol Izquierdo - One of the best experts on this subject based on the ideXlab platform.
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reproductive performance of gilthead seabream sparus aurata Broodstock showing different expression of fatty acyl desaturase 2 and fed two dietary fatty acid profiles
Scientific Reports, 2020Co-Authors: Shajahan Ferosekhan, Serhat Turkmen, Ana M Gomez, J M Afonso, Ramon Fontanillas, Grethe Rosenlund, Sadasivam Kaushik, Marisol IzquierdoAbstract:Previous studies have shown that it is possible to nutritionally program gilthead seabream offspring through fish oil (FO) replacement by vegetable oils (VO) in the Broodstock diet, to improve their ability to grow fast when fed low fish meal (FM) and FO diets during grow-out phase. However, in those studies Broodstock performance was reduced by the VO contained diet. Therefore, the present study aimed to determine if it is possible to replace FO by a mixture of FO and rapeseed oil (RO) with a specific fatty acid profile in Broodstock diets, without altering gilthead seabream Broodstock reproductive performance. Besides, the study also aimed to evaluate the reproductive performance of Broodstock with different expression of fatty acid desaturase 2 gene (fads2) a key enzyme in synthesis of long chain polyunsaturated fatty acids. For that purpose, broodfish having either a high (HD) or low (LD) expression of fads2 were fed for three months during the spawning season with two diets containing different fatty acid profiles and their effects on reproductive hormones, fecundity, sperm and egg quality, egg biochemical composition and fads2 expression were studied. The results showed that blood fads2 expression in females, which tended to be higher than in males, was positively related to plasma 17β-estradiol levels. Moreover, Broodstock with high blood fads2 expression showed a better reproductive performance, in terms of fecundity and sperm and egg quality, which was correlated with female fads2 expression. Our data also showed that it is feasible to reduce ARA, EPA and DHA down to 0.43, 6.6 and 8.4% total fatty acids, respectively, in Broodstock diets designed to induce nutritional programming effects in the offspring without adverse effects on spawning quality. Further studies are being conducted to test the offspring with low FM and FO diets along life span.
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effects of dietary lipid composition and fatty acid desaturase 2 expression in Broodstock gilthead sea bream on lipid metabolism related genes and methylation of the fads2 gene promoter in their offspring
International Journal of Molecular Sciences, 2019Co-Authors: Serhat Turkmen, Erick Perera, M J Zamorano, Paula Simomirabet, Jaume Perezsanchez, Marisol IzquierdoAbstract:Polyunsaturated fatty acids (PUFA) in parental diets play a key role in regulating n-3 LC-PUFA metabolism of the offspring. However, it is not clear whether this metabolic regulation is driven by the precursors presented in the diet or by the parental ability to synthesize them. To elucidate this, Broodstocks of gilthead sea bream with different blood expression levels of fads2, which encodes for the rate-limiting enzyme in the n-3 LC-PUFA synthesis pathway, were fed either a diet supplemented with alpha-linolenic acid (ALA, 18:3n-3) or a control diet. The progenies obtained from these four experimental groups were then challenged with a low LC-PUFA diet at the juvenile stage. Results showed that the offspring from parents with high fads2 expression presented higher growth and improved utilization of low n-3 LC-PUFA diets compared to the offspring from parents with low fads2 expression. Besides, an ALA-rich diet during the gametogenesis caused negative effects on the growth of the offspring. The epigenetic analysis demonstrated that methylation in the promoter of fads2 of the offspring was correlated with the parental fads2 expression levels and type of the Broodstock diet.
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Effect of Broodstock nutrition on reproductive performance of fish
Aquaculture, 2001Co-Authors: Marisol Izquierdo, Hipólito Fernández-palacios, Albert G J TaconAbstract:Abstract In many cultured fish species, particularly in those new for aquaculture, unpredictable and variable reproductive performance is an important limiting factor for the successful mass production of juveniles. An improvement in Broodstock nutrition and feeding has been shown to greatly improve not only egg and sperm quality but also seed production. Gonadal development and fecundity are affected by certain essential dietary nutrients, especially in continuous spawners with short vitellogenic periods. Thus, during the last two decades, more attention has been paid to the level of different nutrients in Broodstock diets. However, studies on Broodstock nutrition are limited and relatively expensive to conduct. Lipid and fatty acid composition of Broodstock diet have been identified as major dietary factors that determine successful reproduction and survival of offspring. Some fish species readily incorporate dietary unsaturated fatty acids into eggs, even during the course of the spawning season. Highly unsaturated fatty acids (HUFA) with 20 or more carbon atoms affect, directly or through their metabolites, fish maturation and steroidogenesis. In some species, HUFA in Broodstock diets increases fecundity, fertilization and egg quality. As in higher vertebrates, vitamin E deficiency affects reproductive performance, causing immature gonads and lower hatching rate and survival of offspring. For example, elevation of dietary α-tocopherol levels has been found to reduce the percentage of abnormal eggs and increase fecundity in the gilthead seabream ( Sparus aurata ). Ascorbic acid has also been shown to play an important role in salmonid reproduction, where the dietary requirement of Broodstock was higher than that of juveniles. Among different feed ingredients, cuttlefish, squid and krill meals are recognized as valuable components of Broodstock diets. The protein component of cuttlefish and squid together with their optimal concentration of HUFA appear to be responsible for their positive effect on reproductive performance. Both polar and nonpolar lipid fractions of raw krill were found to effectively improve egg quality.
Bongrae Kim - One of the best experts on this subject based on the ideXlab platform.
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a taqman real time pcr assay for quantifying white spot syndrome virus wssv infections in wild Broodstock and hatchery reared postlarvae of fleshy shrimp fenneropenaeus chinensis
Aquaculture, 2009Co-Authors: Inkwon Jang, Xianhong Meng, Hyungchul Seo, Yeongrok Cho, Bongrae Kim, Gopalakannan Ayyaru, Jongsheek KimAbstract:Abstract In the present study, a highly sensitive and specific TaqMan real-time PCR was developed to quantify white spot syndrome virus (WSSV) infections in wild Broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis . A total of 159 individuals of wild F. chinensis brooders from 3 locations were captured and 210 postlarvae (PL 1–8 ) were obtained from seven commercial hatcheries in 2007 in South Korea. The WSSV infections in 3 Broodstocks showed a wide range, from 0 to 2.28 × 10 6 (with a mean of 1.50 × 10 4 ) copies ng − 1 of DNA. Out of 159 brooders assayed, 39 (24.5%) were negative and 120 (75.5%) were positive; 153 (96.2%) showed less than 100 copies (mean 10.2 copies), 111 (69.8%) showed less than 10 copies and only 6 individuals (3.8%) showed high infections with a range of 2.36 × 10 2 to 2.28 × 10 6 copies ng − 1 of DNA. In 210 postlarvae, a range of 2.6 to 713.6 (with a mean of 220) copies g − 1 of DNA was observed. The mean WSSV copy number in the postlarvae was 7.9 × 10 5 , which was equivalent to 8.5 × 10 5 copies mg − 1 of postlarvae weight. A total of 87.1% of postlarvae were WSSV positive and except for two hatcheries (H 4 and H 7 ), the postlarvae of all the other five hatcheries were positive. Even postlarvae from the same hatchery, especially of hatcheries H 4 and H 5 , showed a wide range of WSSV infection resulting in higher infections than other hatcheries. There were 34.3% of the postlarvae assayed in the present study that showed very low infection, with less than 10 copies ng − 1 of DNA. Based on our results, it is recommended to pre-screen Broodstock or larvae for selective breeding, stocking in production systems or stock enhancement.
Yeongrok Cho - One of the best experts on this subject based on the ideXlab platform.
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a taqman real time pcr assay for quantifying white spot syndrome virus wssv infections in wild Broodstock and hatchery reared postlarvae of fleshy shrimp fenneropenaeus chinensis
Aquaculture, 2009Co-Authors: Inkwon Jang, Xianhong Meng, Hyungchul Seo, Yeongrok Cho, Bongrae Kim, Gopalakannan Ayyaru, Jongsheek KimAbstract:Abstract In the present study, a highly sensitive and specific TaqMan real-time PCR was developed to quantify white spot syndrome virus (WSSV) infections in wild Broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis . A total of 159 individuals of wild F. chinensis brooders from 3 locations were captured and 210 postlarvae (PL 1–8 ) were obtained from seven commercial hatcheries in 2007 in South Korea. The WSSV infections in 3 Broodstocks showed a wide range, from 0 to 2.28 × 10 6 (with a mean of 1.50 × 10 4 ) copies ng − 1 of DNA. Out of 159 brooders assayed, 39 (24.5%) were negative and 120 (75.5%) were positive; 153 (96.2%) showed less than 100 copies (mean 10.2 copies), 111 (69.8%) showed less than 10 copies and only 6 individuals (3.8%) showed high infections with a range of 2.36 × 10 2 to 2.28 × 10 6 copies ng − 1 of DNA. In 210 postlarvae, a range of 2.6 to 713.6 (with a mean of 220) copies g − 1 of DNA was observed. The mean WSSV copy number in the postlarvae was 7.9 × 10 5 , which was equivalent to 8.5 × 10 5 copies mg − 1 of postlarvae weight. A total of 87.1% of postlarvae were WSSV positive and except for two hatcheries (H 4 and H 7 ), the postlarvae of all the other five hatcheries were positive. Even postlarvae from the same hatchery, especially of hatcheries H 4 and H 5 , showed a wide range of WSSV infection resulting in higher infections than other hatcheries. There were 34.3% of the postlarvae assayed in the present study that showed very low infection, with less than 10 copies ng − 1 of DNA. Based on our results, it is recommended to pre-screen Broodstock or larvae for selective breeding, stocking in production systems or stock enhancement.
