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Franco Ciuchini - One of the best experts on this subject based on the ideXlab platform.
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coombs antiglobulin test using Brucella Abortus 99 as antigen to detect incomplete antibodies induced by b Abortus rb51 vaccine in cattle
Clinical and Vaccine Immunology, 2002Co-Authors: Franco Ciuchini, Rosanna Adone, Paolo PasqualiAbstract:This study showed that vaccination of cattle with Brucella Abortus rough strain RB51 induces incomplete antibodies that can be detectable by a Coombs antiglobulin test using the B. Abortus 99 smooth strain.
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Brucella Abortus rb51 and hot saline extract from Brucella ovis as antigens in a complement fixation test used to detect sheep vaccinated with Brucella Abortus rb51
Clinical and Vaccine Immunology, 2001Co-Authors: Rosanna Adone, Franco CiuchiniAbstract:The efficacy of Brucella Abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. Abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detect B. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovis also reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.
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complement fixation test to assess humoral immunity in cattle and sheep vaccinated with Brucella Abortus rb51
Clinical and Vaccine Immunology, 1999Co-Authors: Rosanna Adone, Franco CiuchiniAbstract:The live attenuated Brucella Abortus strain RB51 is a rifampin-resistant, lipopolysaccharide (LPS) O-chain-deficient mutant of virulent B. Abortus 2308. The reduced O-chain content in RB51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to LPS. The absence of available serologic tests for RB51 also complicates the diagnosis of possible RB51 infections in humans exposed to this strain. The purpose of this study was to evaluate the suitability of a complement fixation (CF) test performed with the rough strain B. Abortus RB51, previously deprived of anticomplementary activity, in detecting anti-B. Abortus RB51 antibodies in cattle and sheep experimentally vaccinated with this strain. The results of this study showed that a CF test with RB51 as the antigen is able to specifically detect antibodies following RB51 vaccination in cattle and sheep. In addition, this method could be a useful tool for detecting B. Abortus RB51 infection in humans.
Domenico Iannelli - One of the best experts on this subject based on the ideXlab platform.
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protective effect of the nramp1 bb genotype against Brucella Abortus in the water buffalo bubalus bubalis
Infection and Immunity, 2007Co-Authors: Rosanna Capparelli, Giorgia Borriello, D Fenizia, Flora Alfano, Sante Roperto, Maria Grazia Amoroso, Antonio C Bianco, F Roperto, Domenico IannelliAbstract:We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella Abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3′ untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella Abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.
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genetic resistance to Brucella Abortus in the water buffalo bubalus bubalis
Infection and Immunity, 2006Co-Authors: Giorgia Borriello, Rosanna Capparelli, Michele Bianco, D Fenizia, Flora Alfano, Federico Capuano, Danilo Ercolini, Antonio Parisi, Sante Roperto, Domenico IannelliAbstract:Brucellosis is a costly disease of water buffaloes (Bubalus bubalis). Latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. We exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis. We tested 231 water buffalo cows for the presence of anti-Brucella Abortus antibodies (by the agglutination and complement fixation tests) and the Nramp1 genotype (by PCR-denaturing gradient gel electrophoresis). When the 231 animals (58 cases and 173 controls) were divided into infected (seropositive) and noninfected (seronegative) groups and the Nramp1 genotypes were compared, the seropositive subjects were 52 out of 167 (31%) in the Nramp1A+ (Nramp1AA or Nramp1AB) group and 6 out of 64 (9.4%) in the Nramp1A− (Nramp1BB) group (odds ratio, 4.37; 95% confidence limits, 1.87 to 10.19; χ2, 11.65 for 1 degree of freedom). Monocytes from Nramp1BB subjects displayed significantly (P < 0.01) higher levels of Nramp1 mRNA than Nramp1AA subjects and also a significantly (P < 0.01) higher ability in controlling the intracellular replication of several Brucella species in vitro. Thus, selection for the Nramp1BB genotype can become a valuable tool for the control of water buffalo brucellosis in the areas where the disease is endemic.
Rosanna Adone - One of the best experts on this subject based on the ideXlab platform.
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coombs antiglobulin test using Brucella Abortus 99 as antigen to detect incomplete antibodies induced by b Abortus rb51 vaccine in cattle
Clinical and Vaccine Immunology, 2002Co-Authors: Franco Ciuchini, Rosanna Adone, Paolo PasqualiAbstract:This study showed that vaccination of cattle with Brucella Abortus rough strain RB51 induces incomplete antibodies that can be detectable by a Coombs antiglobulin test using the B. Abortus 99 smooth strain.
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Brucella Abortus rb51 and hot saline extract from Brucella ovis as antigens in a complement fixation test used to detect sheep vaccinated with Brucella Abortus rb51
Clinical and Vaccine Immunology, 2001Co-Authors: Rosanna Adone, Franco CiuchiniAbstract:The efficacy of Brucella Abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. Abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detect B. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovis also reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.
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complement fixation test to assess humoral immunity in cattle and sheep vaccinated with Brucella Abortus rb51
Clinical and Vaccine Immunology, 1999Co-Authors: Rosanna Adone, Franco CiuchiniAbstract:The live attenuated Brucella Abortus strain RB51 is a rifampin-resistant, lipopolysaccharide (LPS) O-chain-deficient mutant of virulent B. Abortus 2308. The reduced O-chain content in RB51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to LPS. The absence of available serologic tests for RB51 also complicates the diagnosis of possible RB51 infections in humans exposed to this strain. The purpose of this study was to evaluate the suitability of a complement fixation (CF) test performed with the rough strain B. Abortus RB51, previously deprived of anticomplementary activity, in detecting anti-B. Abortus RB51 antibodies in cattle and sheep experimentally vaccinated with this strain. The results of this study showed that a CF test with RB51 as the antigen is able to specifically detect antibodies following RB51 vaccination in cattle and sheep. In addition, this method could be a useful tool for detecting B. Abortus RB51 infection in humans.
Philip H. Elzer - One of the best experts on this subject based on the ideXlab platform.
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the siderophore 2 3 dihydroxybenzoic acid is not required for virulence of Brucella Abortus in balb c mice
Infection and Immunity, 1999Co-Authors: Bryan H. Bellaire, Philip H. Elzer, Cynthia L. Baldwin, Martin R RoopAbstract:2,3-Dihydroxybenzoic acid (DHBA) is the only siderophore described for Brucella, and previous studies suggested that DHBA might contribute to the capacity of these organisms to persist in host macrophages. Employing an isogenic siderophore mutant (ΔentC) constructed from virulent Brucella Abortus 2308, however, we found that production of DHBA is not required for replication in cultured murine macrophages or for the establishment and maintenance of chronic infection in the BALB/c mouse model.
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vaccination with Brucella Abortus rough mutant rb51 protects balb c mice against virulent strains of Brucella Abortus Brucella melitensis and Brucella ovis
Infection and Immunity, 1994Co-Authors: M P Jiménez De Bagüés, Gerhardt G Schurig, Philip H. Elzer, S M Jones, José M. Blasco, F. M. Enright, A J WinterAbstract:Vaccination of BALB/c mice with live Brucella Abortus RB51, a stable rough mutant, produced protection against challenge with virulent strains of Brucella Abortus, Brucella melitensis, and Brucella ovis. Passive-transfer experiments indicated that vaccinated mice were protected against B. Abortus 2308 through cell-mediated immunity, against B. ovis PA through humoral immunity, and against B. melitensis 16M through both forms of immunity. Live bacteria were required for the induction of protective cell-mediated immunity; vaccination with whole killed cells of strain RB51 failed to protect mice against B. Abortus 2308 despite development of good delayed-type hypersensitivity reactions. Protective antibodies against the heterologous species were generated in vaccinated mice primarily through anamnestic responses following challenge infections. Growth of the antigenically unrelated bacterium Listeria monocytogenes in the spleens of vaccinated mice indicated that nonspecific killing by residual activated macrophages contributed minimally to protection. These results encourage the continued investigation of strain RB51 as an alternative vaccine against heterologous Brucella species. However, its usefulness against B. ovis would be limited if, as suggested here, epitopes critical for protective cell-mediated immunity are not shared between B. Abortus and B. ovis.
Giorgia Borriello - One of the best experts on this subject based on the ideXlab platform.
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protective effect of the nramp1 bb genotype against Brucella Abortus in the water buffalo bubalus bubalis
Infection and Immunity, 2007Co-Authors: Rosanna Capparelli, Giorgia Borriello, D Fenizia, Flora Alfano, Sante Roperto, Maria Grazia Amoroso, Antonio C Bianco, F Roperto, Domenico IannelliAbstract:We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella Abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3′ untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella Abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.
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genetic resistance to Brucella Abortus in the water buffalo bubalus bubalis
Infection and Immunity, 2006Co-Authors: Giorgia Borriello, Rosanna Capparelli, Michele Bianco, D Fenizia, Flora Alfano, Federico Capuano, Danilo Ercolini, Antonio Parisi, Sante Roperto, Domenico IannelliAbstract:Brucellosis is a costly disease of water buffaloes (Bubalus bubalis). Latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. We exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis. We tested 231 water buffalo cows for the presence of anti-Brucella Abortus antibodies (by the agglutination and complement fixation tests) and the Nramp1 genotype (by PCR-denaturing gradient gel electrophoresis). When the 231 animals (58 cases and 173 controls) were divided into infected (seropositive) and noninfected (seronegative) groups and the Nramp1 genotypes were compared, the seropositive subjects were 52 out of 167 (31%) in the Nramp1A+ (Nramp1AA or Nramp1AB) group and 6 out of 64 (9.4%) in the Nramp1A− (Nramp1BB) group (odds ratio, 4.37; 95% confidence limits, 1.87 to 10.19; χ2, 11.65 for 1 degree of freedom). Monocytes from Nramp1BB subjects displayed significantly (P < 0.01) higher levels of Nramp1 mRNA than Nramp1AA subjects and also a significantly (P < 0.01) higher ability in controlling the intracellular replication of several Brucella species in vitro. Thus, selection for the Nramp1BB genotype can become a valuable tool for the control of water buffalo brucellosis in the areas where the disease is endemic.
