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Sufang Yang - One of the best experts on this subject based on the ideXlab platform.
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Buffalos bubalus bubalis cloned by nuclear transfer of somatic cells
Biology of Reproduction, 2007Co-Authors: Fenghua Lu, Sufang YangAbstract:Abstract Cloning of Buffalos (Bubalus bubalis) through nuclear transfer is a potential alternative approach in genetic improvement of Buffalos. However, to our knowledge, cloned offspring of Buffalos derived from embryonic, fetal, or somatic cells have not yet been reported. Thus, factors affecting the nuclear transfer of Buffalo somatic cells were examined, and the possibility of cloning Buffalos was explored in the present study. Treatment of Buffalo fibroblasts and granulosa cells with aphidicolin plus serum starvation resulted in more cells being arrested at the G0/G1 phase, the proportion of cells with DNA fragmentation being less, and the number of embryos derived from these cells that developed to blastocysts being greater. In addition, a difference was found in the development of embryos reconstructed with fetal fibroblasts from different individuals (P < 0.001). Forty-two blastocysts derived from granulosa cells and fetal fibroblasts were transferred into 21 recipient swamp Buffalos, and 4 recipi...
T Suteevun - One of the best experts on this subject based on the ideXlab platform.
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epigenetic characteristics of cloned and in vitro fertilized swamp Buffalo bubalus bubalis embryos
Journal of Animal Science, 2006Co-Authors: T Suteevun, Rangsun Parnpai, Sadie L Smith, C C Chang, S Muenthaisong, X C TianAbstract:Swamp Buffalos are becoming endan- gered due to reproductive inefficiencies. This is of con- cern because many countries depend heavily on their products. Somatic cell nuclear transfer (SCNT) is a po- tential strategy for preserving endangered species. To date, SCNT in swamp Buffalo has succeeded in the cre- ation of blastocyst embryos. However, development to term of SCNT swamp Buffalos is extremely limited, and only 1 live birth has been reported. An abnormal epigenetic mechanism is suspected to be the cause of developmental failure, as is also seen in other species. The DNA methylation and histone acetylation are key players in epigenetic modification and display marked variability during embryonic preimplantation develop- ment. Knowledge of epigenetic modifications will aid in solving the developmental problems of SCNT embryos and improving reproductive technology in the swamp Buffalo. The objective of this study was to determine the relationship between preimplantation embryonic
William Weir - One of the best experts on this subject based on the ideXlab platform.
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the african Buffalo parasite theileria sp Buffalo can infect and immortalize cattle leukocytes and encodes divergent orthologues of theileria parva antigen genes
International journal for parasitology. Parasites and wildlife, 2015Co-Authors: Richard P Bishop, William Weir, Johanneke D Hemmink, W I Morrison, Philip G Toye, Tatjana Sitt, P R Spooner, A J Musoke, Robert A Skilton, David OdongoAbstract:African Cape Buffalo (Syncerus caffer) is the wildlife reservoir of multiple species within the apicomplexan protozoan genus Theileria, including Theileria parva which causes East coast fever in cattle. A parasite, which has not yet been formally named, known as Theileria sp. (Buffalo) has been recognized as a potentially distinct species based on rDNA sequence, since 1993. We demonstrate using reverse line blot (RLB) and sequencing of 18S rDNA genes, that in an area where Buffalo and cattle co-graze and there is a heavy tick challenge, T. sp. (Buffalo) can frequently be isolated in culture from cattle leukocytes. We also show that T. sp. (Buffalo), which is genetically very closely related to T. parva, according to 18s rDNA sequence, has a conserved orthologue of the polymorphic immunodominant molecule (PIM) that forms the basis of the diagnostic ELISA used for T. parva serological detection. Closely related orthologues of several CD8 T cell target antigen genes are also shared with T. parva. By contrast, orthologues of the T. parva p104 and the p67 sporozoite surface antigens could not be amplified by PCR from T. sp. (Buffalo), using conserved primers designed from the corresponding T. parva sequences. Collectively the data re-emphasise doubts regarding the value of rDNA sequence data alone for defining apicomplexan species in the absence of additional data. ‘Deep 454 pyrosequencing’ of DNA from two Theileria sporozoite stabilates prepared from Rhipicephalus appendiculatus ticks fed on Buffalo failed to detect T. sp. (Buffalo). This strongly suggests that R. appendiculatus may not be a vector for T. sp. (Buffalo). Collectively, the data provides further evidence that T. sp. (Buffalo). is a distinct species from T. parva.
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haemoparasite prevalence and theileria parva strain diversity in cape Buffalo syncerus caffer in uganda
Veterinary Parasitology, 2011Co-Authors: C A L Oura, Benon B Asiimwe, George W. Lubega, Andy Tait, William WeirAbstract:Cape Buffalo (Syncerus caffer) are considered to be an important reservoir for various tick-borne haemoparasites of veterinary importance. In this study we have compared the haemoparasite carrier prevalence in Buffalo from four geographically isolated national parks in Uganda [Lake Mburo National Park (LMNP), Queen Elizabeth National Park (QENP), Murchison Falls National Park (MFNP) and Kidepo Valley National Park (KVNP)]. Differences were seen in haemoparasite prevalence in Buffalo from the four national parks. All the Buffalo sampled in LMNP were carriers of Theileria parva however, Buffalo from MFNP and KVNP, which are both located in the north of Uganda, were negative for T. parva. Interestingly, 95% of Buffalo in the northern part of QENP were T. parva positive, however all Buffalo sampled in the south of the park were negative. A high multiplicity of infection was recorded in all the Buffalo found to be carrying T. parva, with evidence of at least nine parasite genotypes in some animals. Most of the Buffalo sampled in all four national parks were carriers of T. mutans and T. velifera, however none were carriers of T. taurotragi, Babesia bovis, Babesia bigemina, Ehrlichia bovis or Ehrlichia ruminantium. All the Buffalo sampled from LMNP were positive for T. buffeli and T. sp. (Buffalo) however, Buffalo from the parks in the north of the country (KVNP and MFNP) were negative for these haemoparasites. Anaplasma centrale and Anaplasma marginale were circulating in Buffalo from all four national parks. T. parva gene pools from two geographically separated populations of Buffalo in two of the national parks in Uganda (LMNP and QENP) were compared. The T. parva populations in the two national parks were distinct, indicating that there was limited gene flow between the populations. The results presented highlight the complexity of tick-borne pathogen infections in Buffalo and the significant role that Buffalo may play as reservoir hosts for veterinary haemoparasites that have the potential to cause severe disease in domestic cattle.
Zhongliang Zhao - One of the best experts on this subject based on the ideXlab platform.
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independent maternal origin of chinese swamp Buffalo bubalus bubalis
Animal Genetics, 2007Co-Authors: W Zhang, Zifan Lu, Fan Lu, X Y Yang, Hong Chen, Hong Zhang, Zhongliang ZhaoAbstract:Summary To obtain more knowledge on the origin and genetic diversity of the swamp Buffalo (Bubalus bubalis) in China, the complete mitochondrial D-loop sequences of 119 samples representing seven native types were compared. Two mitochondrial DNA (mtDNA) lineages (lineages A and B) were determined for the Chinese swamp Buffalo. Examination of the diversity patterns suggest that lineage A has undergone a population expansion event. Divergence of lineages A and B was estimated at 18 000 years ago. Combined analyses of mtDNA sequences from Chinese, Indian, Brazilian/Italian and Southeast Asian/Australian Buffalo samples showed independent domestication events in the swamp Buffalo from China and the river Buffalo from the India subcontinent. The spread of swamp and river Buffalo from China and India respectively to mainland Southeast Asia suggests that Southeast Asia is a hybrid zone for Buffalo. Our data support the hypothesis of the evolution of domesticated swamp and river Buffalo from ancestral swamp-like animals. These ancestral animals were extensively distributed across mainland Asia and most likely are represented today by the wild Asian Buffalo (Bubalus arnee).
X C Tian - One of the best experts on this subject based on the ideXlab platform.
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epigenetic characteristics of cloned and in vitro fertilized swamp Buffalo bubalus bubalis embryos
Journal of Animal Science, 2006Co-Authors: T Suteevun, Rangsun Parnpai, Sadie L Smith, C C Chang, S Muenthaisong, X C TianAbstract:Swamp Buffalos are becoming endan- gered due to reproductive inefficiencies. This is of con- cern because many countries depend heavily on their products. Somatic cell nuclear transfer (SCNT) is a po- tential strategy for preserving endangered species. To date, SCNT in swamp Buffalo has succeeded in the cre- ation of blastocyst embryos. However, development to term of SCNT swamp Buffalos is extremely limited, and only 1 live birth has been reported. An abnormal epigenetic mechanism is suspected to be the cause of developmental failure, as is also seen in other species. The DNA methylation and histone acetylation are key players in epigenetic modification and display marked variability during embryonic preimplantation develop- ment. Knowledge of epigenetic modifications will aid in solving the developmental problems of SCNT embryos and improving reproductive technology in the swamp Buffalo. The objective of this study was to determine the relationship between preimplantation embryonic
