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Hyung Joon Cha - One of the best experts on this subject based on the ideXlab platform.
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A comparative study on the Bulk Adhesive strength of the recombinant mussel Adhesive protein fp-3
Biofouling, 2013Co-Authors: Byeongseon Yang, Yoo Seong Choi, Jeong Hyun Seo, Dong Gyun Kang, Hyung Joon ChaAbstract:Mussel Adhesive protein (MAP) type 3 (fp-3) is considered one of the key components for mussel adhesion. However, its Bulk Adhesive strength has not been characterized due to its availability in limited quantities. In the present work, a feasible production (∼47 mg l−1) of recombinant fp-3 was achieved, and its Bulk Adhesive strength was measured for the first time; ∼0.57 MPa for the unmodified form and ∼0.94 and ∼2.28 MPa for the 3,4-dihydroxy-L-phenylalanine (DOPA)-modified form, having a 9.6% yield without and with oxidant treatment, respectively. Furthermore, values for the Bulk Adhesive strength of several DOPA-modified recombinant MAPs were compared. The maximum Adhesive strength of DOPA-modified fp-3 after oxidant treatment was stronger than that of type 5 (fp-5), which has a 6.2% modification yield, and was comparable to that of hybrid types fp-131 and fp-151, which have similar yields (∼5%). The strong Bulk Adhesive property of recombinant fp-3 demonstrates its potential use as a promising bioadh...
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Recombinant mussel Adhesive protein fp-5 (MAP fp-5) as a Bulk bioAdhesive and surface coating material
Biofouling, 2011Co-Authors: Yoo Seong Choi, Seonghye Lim, Yun Jung Yang, Dong Gyun Kang, Chang Sup Kim, Hyung Joon ChaAbstract:Mussel Adhesive proteins (MAPs) attach to all types of inorganic and organic surfaces, even in wet environments. MAP of type 5 (fp-5), in particular, has been considered as a key Adhesive material. However, the low availability of fp-5 has hampered its biochemical characterization and practical applications. Here, soluble recombinant fp-5 is mass-produced in Escherichia coli. Tyrosinase-modified recombinant fp-5 showed ∼1.11 MPa Adhesive shear strength, which is the first report of a Bulk-scale Adhesive force measurement for purified recombinant of natural MAP type. Surface coatings were also performed through simple dip-coating of various objects. In addition, complex coacervate using recombinant fp-5 and hyaluronic acid was prepared as an efficient Adhesive formulation, which greatly improved the Bulk Adhesive strength. Collectively, it is expected that this work will enhance basic understanding of mussel adhesion and that recombinant fp-5 can be successfully used as a realistic Bulk-scale bioAdhesive and an efficient surface coating material.
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the Adhesive properties of coacervated recombinant hybrid mussel Adhesive proteins
Biomaterials, 2010Co-Authors: Seonghye Lim, Young Hoon Song, Yoo Seong Choi, Dong Gyun Kang, Hyung Joon ChaAbstract:Marine mussels attach to substrates using Adhesive proteins. It has been suggested that complex coacervation (liquid-liquid phase separation via concentration) might be involved in the highly condensed and non-water dispersed adhesion process of mussel Adhesive proteins (MAPs). However, as purified natural MAPs are difficult to obtain, it has not been possible to experimentally validate the coacervation model. In the present work, we demonstrate complex coacervation in a system including recombinant MAPs and hyaluronic acid (HA). Our recombinant hybrid MAPs, fp-151 and fp-131, can be produced in large quantities, and are readily purified. We observed successful complex coacervation using cationic fp-151 or fp-131, and an anionic HA partner. Importantly, we found that highly condensed complex coacervates significantly increased the Bulk Adhesive strength of MAPs in both dry and wet environments. In addition, oil droplets were successfully engulfed using a MAP-based interfacial coacervation process, to form microencapsulated particles. Collectively, our results indicate that a complex coacervation system based on MAPs shows superior Adhesive properties, combined with additional valuable features including liquid/liquid phase separation and appropriate viscoelasticity. Our microencapsulation system could be useful in the development of new Adhesive biomaterials, including self-Adhesive microencapsulated drug carriers, for use in biotechnological and biomedical applications.
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Bulk Adhesive strength of recombinant hybrid mussel Adhesive protein.
Biofouling, 2009Co-Authors: Hyung Joon Cha, Seonghye Lim, James D. White, Cristina R. Matos-pérez, Jonathan J WilkerAbstract:Mussel Adhesive proteins (MAPs) have received increased attention as potential biomedical and environmental friendly Adhesives. However, practical application of MAPs has been severely limited by u...
Yoo Seong Choi - One of the best experts on this subject based on the ideXlab platform.
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Mussel Adhesive protein as an environmentally-friendly harmless wood furniture Adhesive
International Journal of Adhesion and Adhesives, 2016Co-Authors: Young Hoon Song, Yoo Seong Choi, Bong-hyuk ChoiAbstract:Abstract Recent Adhesive technologies have focused on the development of high-quality and eco-friendly Adhesives. Thus, there is a gradual shift from the currently used chemical-based Adhesives toward harmless Adhesives with improved quality and performance. Here, we evaluated the potential use of bacteria-produced recombinant mussel Adhesive protein (MAP) as a harmless wood furniture Adhesive. We formulated a MAP wood Adhesive as an inclusion body type for economical preparation, and we confirmed its harmlessness through the non-detection of volatile organic compounds and heavy metals. The formulated MAP showed sufficiently strong Bulk Adhesive strength for the dried gluing of wood adherends. We also found that the formulated MAP wood Adhesive exhibits robust adhesion in various environmental conditions, including open assembly times, incubation times, temperatures, and humidity levels. In summary, the developed recombinant MAP could be successfully used as a promising environmentally-friendly, harmless wood furniture Adhesive.
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A comparative study on the Bulk Adhesive strength of the recombinant mussel Adhesive protein fp-3
Biofouling, 2013Co-Authors: Byeongseon Yang, Yoo Seong Choi, Jeong Hyun Seo, Dong Gyun Kang, Hyung Joon ChaAbstract:Mussel Adhesive protein (MAP) type 3 (fp-3) is considered one of the key components for mussel adhesion. However, its Bulk Adhesive strength has not been characterized due to its availability in limited quantities. In the present work, a feasible production (∼47 mg l−1) of recombinant fp-3 was achieved, and its Bulk Adhesive strength was measured for the first time; ∼0.57 MPa for the unmodified form and ∼0.94 and ∼2.28 MPa for the 3,4-dihydroxy-L-phenylalanine (DOPA)-modified form, having a 9.6% yield without and with oxidant treatment, respectively. Furthermore, values for the Bulk Adhesive strength of several DOPA-modified recombinant MAPs were compared. The maximum Adhesive strength of DOPA-modified fp-3 after oxidant treatment was stronger than that of type 5 (fp-5), which has a 6.2% modification yield, and was comparable to that of hybrid types fp-131 and fp-151, which have similar yields (∼5%). The strong Bulk Adhesive property of recombinant fp-3 demonstrates its potential use as a promising bioadh...
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Recombinant mussel Adhesive protein fp-5 (MAP fp-5) as a Bulk bioAdhesive and surface coating material
Biofouling, 2011Co-Authors: Yoo Seong Choi, Seonghye Lim, Yun Jung Yang, Dong Gyun Kang, Chang Sup Kim, Hyung Joon ChaAbstract:Mussel Adhesive proteins (MAPs) attach to all types of inorganic and organic surfaces, even in wet environments. MAP of type 5 (fp-5), in particular, has been considered as a key Adhesive material. However, the low availability of fp-5 has hampered its biochemical characterization and practical applications. Here, soluble recombinant fp-5 is mass-produced in Escherichia coli. Tyrosinase-modified recombinant fp-5 showed ∼1.11 MPa Adhesive shear strength, which is the first report of a Bulk-scale Adhesive force measurement for purified recombinant of natural MAP type. Surface coatings were also performed through simple dip-coating of various objects. In addition, complex coacervate using recombinant fp-5 and hyaluronic acid was prepared as an efficient Adhesive formulation, which greatly improved the Bulk Adhesive strength. Collectively, it is expected that this work will enhance basic understanding of mussel adhesion and that recombinant fp-5 can be successfully used as a realistic Bulk-scale bioAdhesive and an efficient surface coating material.
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the Adhesive properties of coacervated recombinant hybrid mussel Adhesive proteins
Biomaterials, 2010Co-Authors: Seonghye Lim, Young Hoon Song, Yoo Seong Choi, Dong Gyun Kang, Hyung Joon ChaAbstract:Marine mussels attach to substrates using Adhesive proteins. It has been suggested that complex coacervation (liquid-liquid phase separation via concentration) might be involved in the highly condensed and non-water dispersed adhesion process of mussel Adhesive proteins (MAPs). However, as purified natural MAPs are difficult to obtain, it has not been possible to experimentally validate the coacervation model. In the present work, we demonstrate complex coacervation in a system including recombinant MAPs and hyaluronic acid (HA). Our recombinant hybrid MAPs, fp-151 and fp-131, can be produced in large quantities, and are readily purified. We observed successful complex coacervation using cationic fp-151 or fp-131, and an anionic HA partner. Importantly, we found that highly condensed complex coacervates significantly increased the Bulk Adhesive strength of MAPs in both dry and wet environments. In addition, oil droplets were successfully engulfed using a MAP-based interfacial coacervation process, to form microencapsulated particles. Collectively, our results indicate that a complex coacervation system based on MAPs shows superior Adhesive properties, combined with additional valuable features including liquid/liquid phase separation and appropriate viscoelasticity. Our microencapsulation system could be useful in the development of new Adhesive biomaterials, including self-Adhesive microencapsulated drug carriers, for use in biotechnological and biomedical applications.
Seonghye Lim - One of the best experts on this subject based on the ideXlab platform.
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Bulk Adhesive strength of recombinant hybrid mussel Adhesive protein
'Informa UK Limited', 2018Co-Authors: Hj Cha, Ds Hwang, Seonghye Lim, James D. White, Cristina R. Matos-pérez, Jonathan J WilkerAbstract:Mussel Adhesive proteins (MAPs) have received increased attention as potential biomedical and environmental friendly Adhesives. However, practical application of MAPs has been severely limited by uneconomical extraction and unsuccessful genetic production. Developing new Adhesives requires access to large quantities of material and demonstrations of Bulk mechanical properties. Previously, the authors designed fp-151, a fusion protein comprised of six MAP type 1 (fp-1) decapeptide repeats at each MAP type 5 (fp-5) terminus and successfully expressed it in Escherichia coli. This recombinant hybrid protein exhibited high-level expression, a simple purification and high biocompatibility as well as strong Adhesive ability on a micro-scale. In the present work, investigations on the Bulk Adhesive properties of semi-purified (90% purity) fusion fp-151 were performed in air. The unmodified recombinant fp-151, as expressed, contains tyrosine residues and showed significant shear-Adhesive forces (0.33MPa). Adhesion strength increased (0.45MPa) after enzymatic oxidation of tyrosine residues to l-3,4-dihydroxyphenylalanine (DOPA) groups. Addition of cross-linkers such as iron(III), manganese(III) and periodate (IO4-) generally enhanced adhesion, although too much addition decreased adhesion. Among the three cross-linking reagents examined, the non-metallic oxidant periodate showed the highest shear-Adhesive forces (0.86MPa). In addition, it was found that Adhesive strengths could be increased by adding weights to the samples. The highest adhesion strength found was that of DOPA-containing fp-151 cross-linked with periodate and having weights applied to the samples (1.06MPa). Taken together, the first Bulk-scale Adhesive force measurements are presented for an expressed recombinant hybrid mussel Adhesive protein.X1393
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Recombinant mussel Adhesive protein fp-5 (MAP fp-5) as a Bulk bioAdhesive and surface coating material
Biofouling, 2011Co-Authors: Yoo Seong Choi, Seonghye Lim, Yun Jung Yang, Dong Gyun Kang, Chang Sup Kim, Hyung Joon ChaAbstract:Mussel Adhesive proteins (MAPs) attach to all types of inorganic and organic surfaces, even in wet environments. MAP of type 5 (fp-5), in particular, has been considered as a key Adhesive material. However, the low availability of fp-5 has hampered its biochemical characterization and practical applications. Here, soluble recombinant fp-5 is mass-produced in Escherichia coli. Tyrosinase-modified recombinant fp-5 showed ∼1.11 MPa Adhesive shear strength, which is the first report of a Bulk-scale Adhesive force measurement for purified recombinant of natural MAP type. Surface coatings were also performed through simple dip-coating of various objects. In addition, complex coacervate using recombinant fp-5 and hyaluronic acid was prepared as an efficient Adhesive formulation, which greatly improved the Bulk Adhesive strength. Collectively, it is expected that this work will enhance basic understanding of mussel adhesion and that recombinant fp-5 can be successfully used as a realistic Bulk-scale bioAdhesive and an efficient surface coating material.
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the Adhesive properties of coacervated recombinant hybrid mussel Adhesive proteins
Biomaterials, 2010Co-Authors: Seonghye Lim, Young Hoon Song, Yoo Seong Choi, Dong Gyun Kang, Hyung Joon ChaAbstract:Marine mussels attach to substrates using Adhesive proteins. It has been suggested that complex coacervation (liquid-liquid phase separation via concentration) might be involved in the highly condensed and non-water dispersed adhesion process of mussel Adhesive proteins (MAPs). However, as purified natural MAPs are difficult to obtain, it has not been possible to experimentally validate the coacervation model. In the present work, we demonstrate complex coacervation in a system including recombinant MAPs and hyaluronic acid (HA). Our recombinant hybrid MAPs, fp-151 and fp-131, can be produced in large quantities, and are readily purified. We observed successful complex coacervation using cationic fp-151 or fp-131, and an anionic HA partner. Importantly, we found that highly condensed complex coacervates significantly increased the Bulk Adhesive strength of MAPs in both dry and wet environments. In addition, oil droplets were successfully engulfed using a MAP-based interfacial coacervation process, to form microencapsulated particles. Collectively, our results indicate that a complex coacervation system based on MAPs shows superior Adhesive properties, combined with additional valuable features including liquid/liquid phase separation and appropriate viscoelasticity. Our microencapsulation system could be useful in the development of new Adhesive biomaterials, including self-Adhesive microencapsulated drug carriers, for use in biotechnological and biomedical applications.
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Bulk Adhesive strength of recombinant hybrid mussel Adhesive protein.
Biofouling, 2009Co-Authors: Hyung Joon Cha, Seonghye Lim, James D. White, Cristina R. Matos-pérez, Jonathan J WilkerAbstract:Mussel Adhesive proteins (MAPs) have received increased attention as potential biomedical and environmental friendly Adhesives. However, practical application of MAPs has been severely limited by u...
Dong Gyun Kang - One of the best experts on this subject based on the ideXlab platform.
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A comparative study on the Bulk Adhesive strength of the recombinant mussel Adhesive protein fp-3
Biofouling, 2013Co-Authors: Byeongseon Yang, Yoo Seong Choi, Jeong Hyun Seo, Dong Gyun Kang, Hyung Joon ChaAbstract:Mussel Adhesive protein (MAP) type 3 (fp-3) is considered one of the key components for mussel adhesion. However, its Bulk Adhesive strength has not been characterized due to its availability in limited quantities. In the present work, a feasible production (∼47 mg l−1) of recombinant fp-3 was achieved, and its Bulk Adhesive strength was measured for the first time; ∼0.57 MPa for the unmodified form and ∼0.94 and ∼2.28 MPa for the 3,4-dihydroxy-L-phenylalanine (DOPA)-modified form, having a 9.6% yield without and with oxidant treatment, respectively. Furthermore, values for the Bulk Adhesive strength of several DOPA-modified recombinant MAPs were compared. The maximum Adhesive strength of DOPA-modified fp-3 after oxidant treatment was stronger than that of type 5 (fp-5), which has a 6.2% modification yield, and was comparable to that of hybrid types fp-131 and fp-151, which have similar yields (∼5%). The strong Bulk Adhesive property of recombinant fp-3 demonstrates its potential use as a promising bioadh...
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Recombinant mussel Adhesive protein fp-5 (MAP fp-5) as a Bulk bioAdhesive and surface coating material
Biofouling, 2011Co-Authors: Yoo Seong Choi, Seonghye Lim, Yun Jung Yang, Dong Gyun Kang, Chang Sup Kim, Hyung Joon ChaAbstract:Mussel Adhesive proteins (MAPs) attach to all types of inorganic and organic surfaces, even in wet environments. MAP of type 5 (fp-5), in particular, has been considered as a key Adhesive material. However, the low availability of fp-5 has hampered its biochemical characterization and practical applications. Here, soluble recombinant fp-5 is mass-produced in Escherichia coli. Tyrosinase-modified recombinant fp-5 showed ∼1.11 MPa Adhesive shear strength, which is the first report of a Bulk-scale Adhesive force measurement for purified recombinant of natural MAP type. Surface coatings were also performed through simple dip-coating of various objects. In addition, complex coacervate using recombinant fp-5 and hyaluronic acid was prepared as an efficient Adhesive formulation, which greatly improved the Bulk Adhesive strength. Collectively, it is expected that this work will enhance basic understanding of mussel adhesion and that recombinant fp-5 can be successfully used as a realistic Bulk-scale bioAdhesive and an efficient surface coating material.
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the Adhesive properties of coacervated recombinant hybrid mussel Adhesive proteins
Biomaterials, 2010Co-Authors: Seonghye Lim, Young Hoon Song, Yoo Seong Choi, Dong Gyun Kang, Hyung Joon ChaAbstract:Marine mussels attach to substrates using Adhesive proteins. It has been suggested that complex coacervation (liquid-liquid phase separation via concentration) might be involved in the highly condensed and non-water dispersed adhesion process of mussel Adhesive proteins (MAPs). However, as purified natural MAPs are difficult to obtain, it has not been possible to experimentally validate the coacervation model. In the present work, we demonstrate complex coacervation in a system including recombinant MAPs and hyaluronic acid (HA). Our recombinant hybrid MAPs, fp-151 and fp-131, can be produced in large quantities, and are readily purified. We observed successful complex coacervation using cationic fp-151 or fp-131, and an anionic HA partner. Importantly, we found that highly condensed complex coacervates significantly increased the Bulk Adhesive strength of MAPs in both dry and wet environments. In addition, oil droplets were successfully engulfed using a MAP-based interfacial coacervation process, to form microencapsulated particles. Collectively, our results indicate that a complex coacervation system based on MAPs shows superior Adhesive properties, combined with additional valuable features including liquid/liquid phase separation and appropriate viscoelasticity. Our microencapsulation system could be useful in the development of new Adhesive biomaterials, including self-Adhesive microencapsulated drug carriers, for use in biotechnological and biomedical applications.
A D Crocombe - One of the best experts on this subject based on the ideXlab platform.
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the strength prediction of Adhesive single lap joints exposed to long term loading in a hostile environment
International Journal of Adhesion and Adhesives, 2014Co-Authors: A D Crocombe, Xiao Han, S N R AnwarAbstract:This work is concerned with investigating the residual static strength of Adhesively bonded joints after long-term exposure to a combined mechanical-hygro-thermal environment. Associated experimental data are also reported. The degradation process of the joints was modelled using a fully-coupled approach, with the moisture concentration affecting the stress distribution and the stress state affecting the moisture diffusion analyses simultaneously. A bilinear cohesive zone model was then used to implement the progressive damage FE analysis of the quasi-statically loaded joints following the ageing phase. This model is degraded using the damage factors (creep strain and moisture uptake) accumulated over the ageing process and calibrated against the experimental results from static tests on the Bulk Adhesive. Predicted and experimentally-measured quasi-static responses for the aged Adhesive joints were found to be in good agreement.
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experimental and numerical investigation of the static response of environmentally aged Adhesively bonded joints
International Journal of Adhesion and Adhesives, 2013Co-Authors: Sugiman Sugiman, A D Crocombe, I A AschroftAbstract:Abstract The aim of this research is to investigate the effect of moisture on the static response of Adhesively bonded monolithic single lap joints and laminated doublers loaded in bending. All joints were made of aluminium alloy Al 2024-T3 bonded using epoxy film Adhesive FM 73M OST. The joints were aged in deionised water at a temperature of 50 °C for up to 2 years exposure. The use of different widths of specimen (5 mm for monolithic single lap joints and 15 mm for laminated doublers) allowed both full and partial saturation of the Adhesive layer. The Bulk Adhesive has been characterised to obtain the coefficient of moisture diffusion, the coefficient of thermal and moisture expansion and the moisture dependent mechanical properties. The testing results showed that the mechanical properties degraded in a linear way with the moisture content. The residual strength after exposure decreased with increasing moisture content (exposure time) and tended to level off towards saturation. The damage evolution and failure of the joint has been successfully monitored using the backface strain technique and in-situ video microscopy. Progressive damage finite element modelling using a moisture dependent, bilinear traction-separation law has been undertaken to predict the residual strength. Residual stresses due to thermal and swelling strains in the Adhesive layer have been included; however their effect on the predicted static strength was not significant. Good agreement was found between the predicted residual strength and the experimental result.
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strength prediction of Adhesive joints after cyclic moisture conditioning using a cohesive zone model
Engineering Fracture Mechanics, 2011Co-Authors: Aamir Mubashar, I A Ashcroft, Gary W Critchlow, A D CrocombeAbstract:Abstract This paper presents a methodology to predict the strength of Adhesive joints under variable moisture conditions. The moisture uptake in Adhesive joints was determined using a history dependent moisture prediction methodology where diffusion coefficients were based on experimental cyclic moisture uptake of Bulk Adhesive samples. The predicted moisture concentrations and moisture diffusion history were used in a structural analysis with a cohesive zone model to predict damage and failure of the joints. A moisture concentration and moisture history dependent bilinear cohesive zone law was used. The methodology was used to determine the damage and failure in aluminium alloy – epoxy Adhesive single lap joints, conditioned at 50 °C and good predictions of failure load were observed. The damage in the Adhesive joints decreased the load carrying capacity before reaching the failure load and a nonlinear relationship between the load and displacement was observed. Changes in crack initiation and crack propagation were also observed between different types of joints. The presented methodology is generic and may be applied to different types of Adhesive joint and Adhesive.
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Evaluation of Fatigue Damage in Adhesive Bonding: Part 2: Single Lap Joint
Journal of Adhesion Science and Technology, 2010Co-Authors: M. Abdel Wahab, Irfan Hilmy, Ian Ashcroft, A D CrocombeAbstract:The damage parameters for crack initiation in a single lap joint (SLJ) are determined by combining continuous damage mechanics, finite element analysis (FEA) and experimental fatigue data. Even though a SLJ has a simple configuration, the stresses in the Adhesive region are quite complex and exhibit multi-axial states. Such a condition leads to the need to introduce a general value for the triaxiality function in the damage evolution law rather than using a triaxiality function which equals unity, as in the case of a uni-axial stress state, e.g., the Bulk Adhesive test specimen presented in Part 1 of this paper. The effect of stress singularity, due to the presence of corners at edges, also contributes to the complex state of stress and to the variability of the triaxiality function along the Adhesive layer in a SLJ. The damage parameters A and β determined in Part 1 for Bulk Adhesive are now extended to take into account the multi-axial stress state in the Adhesive layer, as calculated from FEA.
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evaluation of fatigue damage in Adhesive bonding part 1 Bulk Adhesive
Journal of Adhesion Science and Technology, 2010Co-Authors: Abdel M Wahab, Irfan Hilmy, I A Ashcroft, A D CrocombeAbstract:The measurement of fatigue damage in Adhesive bonding has been investigated. Bulk Adhesive was used in this study for two reasons: the stress distribution of Adhesives in Bulk is simpler to investigate than Adhesives in joints; and the specimen dimensions met fatigue test standards. Bulk Adhesive was made from a film type of epoxy resin. In general, the characteristics and the behaviour of Bulk Adhesive may differ from Adhesive in joint because of the presence of voids and the constraints imposed by the substrates. Low cycle fatigue tests with a load amplitude ratio of 0.1 at a frequency of 5 Hz were performed to determine the damage as a function of the number of cycles. Damage curves, i.e., the evolution of the damage variable as a function of number of cycles, were derived and plotted using an isotropic damage equation. Damage was evaluated using the decrease of stress range during the lifecycles of a constant displacement amplitude test. It was found that the damage curves were well fitted by a low cy...
