The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Peter M. Vogt - One of the best experts on this subject based on the ideXlab platform.
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insight in microcirculation and histomorphology during Burn Shock treatment using in vivo confocal laser scanning microscopy
Journal of Critical Care, 2010Co-Authors: M.a. Altintas, Ahmet Ali Altintas, Merlin Guggenheim, Matthias Aust, Andreas D. Niederbichler, Karsten Knobloch, Peter M. VogtAbstract:Abstract Purpose Microcirculatory disturbances are well known during Shock; however, the accompanied histomorphological alterations are widely unknown. We used high resolution confocal-laser-scanning microscopy for the evaluation of microcirculation and histomorphology during Burn Shock treatment. Methods Confocal-laser-scanning microscopy was performed in 10 Burn Shock patients (4 women, 6 men; aged 40.6 ± 11.4 years, Burn extent >20% body surface area) initially and 24 hours after Shock resuscitation. Ten matched hemodynamic stable Burn intensive care unit patients served as controls. The following parameters were evaluated: quantitative blood cell flow, cell size of the granular layer, basal layer thickness, and epidermal thickness. Results Quantitative blood cell flow in controls was 62.45 ± 3.39 cells per minute. Burn Shock significantly reduced blood cell flow to 37.27 ± 3.64 cells per minute; fluid resuscitation effectively restored baseline blood flow (65.18 ± 3.76 cells per minute) after 24 hours. Granular cell size was 793.61 ± 41.58 μ m 2 in controls vs 644.27 ± 42.96 μ m 2 during Burn Shock. Post resuscitation granular cell size measured 932.74 ± 38.83 μ m 2 . Basal layer thickness was 14.84 ± 0.59 μ m in controls, 13.26 ± 0.54 μ m in Burn patients at admission and before resuscitation, and 17.50 ± 0.46 μ m after resuscitation. Epidermal thickness in control patients was 49.60 ± 2.36 μ m, 37.83 ± 2.47 μ m in Burn patients at admission and 69.50 ± 3.18 μ m after resuscitation. Conclusions Confocal-laser-scanning microscopy provides a noninvasive tool for simultaneous evaluation of microcirculation and tissue histomorphology. It may help to assess the adequacy of and response to resuscitation of Burn patients early after trauma.
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Insight in microcirculation and histomorphology during Burn Shock treatment using in vivo confocal-laser-scanning microscopy ☆,☆☆
Journal of critical care, 2009Co-Authors: M.a. Altintas, Ahmet Ali Altintas, Merlin Guggenheim, Matthias Aust, Andreas D. Niederbichler, Karsten Knobloch, Peter M. VogtAbstract:Abstract Purpose Microcirculatory disturbances are well known during Shock; however, the accompanied histomorphological alterations are widely unknown. We used high resolution confocal-laser-scanning microscopy for the evaluation of microcirculation and histomorphology during Burn Shock treatment. Methods Confocal-laser-scanning microscopy was performed in 10 Burn Shock patients (4 women, 6 men; aged 40.6 ± 11.4 years, Burn extent >20% body surface area) initially and 24 hours after Shock resuscitation. Ten matched hemodynamic stable Burn intensive care unit patients served as controls. The following parameters were evaluated: quantitative blood cell flow, cell size of the granular layer, basal layer thickness, and epidermal thickness. Results Quantitative blood cell flow in controls was 62.45 ± 3.39 cells per minute. Burn Shock significantly reduced blood cell flow to 37.27 ± 3.64 cells per minute; fluid resuscitation effectively restored baseline blood flow (65.18 ± 3.76 cells per minute) after 24 hours. Granular cell size was 793.61 ± 41.58 μ m 2 in controls vs 644.27 ± 42.96 μ m 2 during Burn Shock. Post resuscitation granular cell size measured 932.74 ± 38.83 μ m 2 . Basal layer thickness was 14.84 ± 0.59 μ m in controls, 13.26 ± 0.54 μ m in Burn patients at admission and before resuscitation, and 17.50 ± 0.46 μ m after resuscitation. Epidermal thickness in control patients was 49.60 ± 2.36 μ m, 37.83 ± 2.47 μ m in Burn patients at admission and 69.50 ± 3.18 μ m after resuscitation. Conclusions Confocal-laser-scanning microscopy provides a noninvasive tool for simultaneous evaluation of microcirculation and tissue histomorphology. It may help to assess the adequacy of and response to resuscitation of Burn patients early after trauma.
M.a. Altintas - One of the best experts on this subject based on the ideXlab platform.
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insight in microcirculation and histomorphology during Burn Shock treatment using in vivo confocal laser scanning microscopy
Journal of Critical Care, 2010Co-Authors: M.a. Altintas, Ahmet Ali Altintas, Merlin Guggenheim, Matthias Aust, Andreas D. Niederbichler, Karsten Knobloch, Peter M. VogtAbstract:Abstract Purpose Microcirculatory disturbances are well known during Shock; however, the accompanied histomorphological alterations are widely unknown. We used high resolution confocal-laser-scanning microscopy for the evaluation of microcirculation and histomorphology during Burn Shock treatment. Methods Confocal-laser-scanning microscopy was performed in 10 Burn Shock patients (4 women, 6 men; aged 40.6 ± 11.4 years, Burn extent >20% body surface area) initially and 24 hours after Shock resuscitation. Ten matched hemodynamic stable Burn intensive care unit patients served as controls. The following parameters were evaluated: quantitative blood cell flow, cell size of the granular layer, basal layer thickness, and epidermal thickness. Results Quantitative blood cell flow in controls was 62.45 ± 3.39 cells per minute. Burn Shock significantly reduced blood cell flow to 37.27 ± 3.64 cells per minute; fluid resuscitation effectively restored baseline blood flow (65.18 ± 3.76 cells per minute) after 24 hours. Granular cell size was 793.61 ± 41.58 μ m 2 in controls vs 644.27 ± 42.96 μ m 2 during Burn Shock. Post resuscitation granular cell size measured 932.74 ± 38.83 μ m 2 . Basal layer thickness was 14.84 ± 0.59 μ m in controls, 13.26 ± 0.54 μ m in Burn patients at admission and before resuscitation, and 17.50 ± 0.46 μ m after resuscitation. Epidermal thickness in control patients was 49.60 ± 2.36 μ m, 37.83 ± 2.47 μ m in Burn patients at admission and 69.50 ± 3.18 μ m after resuscitation. Conclusions Confocal-laser-scanning microscopy provides a noninvasive tool for simultaneous evaluation of microcirculation and tissue histomorphology. It may help to assess the adequacy of and response to resuscitation of Burn patients early after trauma.
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Insight in microcirculation and histomorphology during Burn Shock treatment using in vivo confocal-laser-scanning microscopy ☆,☆☆
Journal of critical care, 2009Co-Authors: M.a. Altintas, Ahmet Ali Altintas, Merlin Guggenheim, Matthias Aust, Andreas D. Niederbichler, Karsten Knobloch, Peter M. VogtAbstract:Abstract Purpose Microcirculatory disturbances are well known during Shock; however, the accompanied histomorphological alterations are widely unknown. We used high resolution confocal-laser-scanning microscopy for the evaluation of microcirculation and histomorphology during Burn Shock treatment. Methods Confocal-laser-scanning microscopy was performed in 10 Burn Shock patients (4 women, 6 men; aged 40.6 ± 11.4 years, Burn extent >20% body surface area) initially and 24 hours after Shock resuscitation. Ten matched hemodynamic stable Burn intensive care unit patients served as controls. The following parameters were evaluated: quantitative blood cell flow, cell size of the granular layer, basal layer thickness, and epidermal thickness. Results Quantitative blood cell flow in controls was 62.45 ± 3.39 cells per minute. Burn Shock significantly reduced blood cell flow to 37.27 ± 3.64 cells per minute; fluid resuscitation effectively restored baseline blood flow (65.18 ± 3.76 cells per minute) after 24 hours. Granular cell size was 793.61 ± 41.58 μ m 2 in controls vs 644.27 ± 42.96 μ m 2 during Burn Shock. Post resuscitation granular cell size measured 932.74 ± 38.83 μ m 2 . Basal layer thickness was 14.84 ± 0.59 μ m in controls, 13.26 ± 0.54 μ m in Burn patients at admission and before resuscitation, and 17.50 ± 0.46 μ m after resuscitation. Epidermal thickness in control patients was 49.60 ± 2.36 μ m, 37.83 ± 2.47 μ m in Burn patients at admission and 69.50 ± 3.18 μ m after resuscitation. Conclusions Confocal-laser-scanning microscopy provides a noninvasive tool for simultaneous evaluation of microcirculation and tissue histomorphology. It may help to assess the adequacy of and response to resuscitation of Burn patients early after trauma.
Julien Textoris - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome modulation by hydrocortisone in severe Burn Shock: ancillary analysis of a prospective randomized trial
Critical Care, 2017Co-Authors: Jonathan Plassais, Marie-angélique Cazalis, Diane Le Quang, Fabienne Venet, Alexandre Pachot, Guillaume Monneret, Sylvie Tissot, Julien TextorisAbstract:BackgroundDespite shortening vasopressor use in Shock, hydrocortisone administration remains controversial, with potential harm to the immune system. Few studies have assessed the impact of hydrocortisone on the transcriptional response in Shock, and we are lacking data on Burn Shock. Our objective was to assess the hydrocortisone-induced transcriptional modulation in severe Burn Shock, particularly modulation of the immune response.MethodsWe collected whole blood samples during a randomized controlled trial assessing the efficacy of hydrocortisone administration in Burn Shock. Using whole genome microarrays, we first compared Burn patients (n = 32) from the placebo group to healthy volunteers to describe the transcriptional modulation induced by Burn Shock over the first week. Then we compared Burn patients randomized for either hydrocortisone administration or placebo, to assess hydrocortisone-induced modulation.ResultsStudy groups were similar in terms of severity and major outcomes, but Shock duration was significantly reduced in the hydrocortisone group. Many genes (n = 1687) were differentially expressed between Burn patients and healthy volunteers, with 85% of them exhibiting a profound and persistent modulation over seven days. Interestingly, we showed that hydrocortisone enhanced the Shock-associated repression of adaptive, but also innate immunity.ConclusionsWe found that the initial host response to Burn Shock encompasses wide and persistent modulation of gene expression, with profound modulation of pathways associated with metabolism and immunity. Importantly, hydrocortisone administration may worsen the immunosuppression associated with severe injury. These data should be taken into account in the risk ratio of hydrocortisone administration in patients with inflammatory Shock.Trial registrationClinicalTrials.gov, NCT00149123. Registered on 6 September 2005.
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Transcriptome modulation by hydrocortisone in severe Burn Shock: ancillary analysis of a prospective randomized trial.
Critical care (London England), 2017Co-Authors: Jonathan Plassais, Marie-angélique Cazalis, Diane Le Quang, Fabienne Venet, Alexandre Pachot, Guillaume Monneret, Sylvie Tissot, Julien TextorisAbstract:Despite shortening vasopressor use in Shock, hydrocortisone administration remains controversial, with potential harm to the immune system. Few studies have assessed the impact of hydrocortisone on the transcriptional response in Shock, and we are lacking data on Burn Shock. Our objective was to assess the hydrocortisone-induced transcriptional modulation in severe Burn Shock, particularly modulation of the immune response. We collected whole blood samples during a randomized controlled trial assessing the efficacy of hydrocortisone administration in Burn Shock. Using whole genome microarrays, we first compared Burn patients (n = 32) from the placebo group to healthy volunteers to describe the transcriptional modulation induced by Burn Shock over the first week. Then we compared Burn patients randomized for either hydrocortisone administration or placebo, to assess hydrocortisone-induced modulation. Study groups were similar in terms of severity and major outcomes, but Shock duration was significantly reduced in the hydrocortisone group. Many genes (n = 1687) were differentially expressed between Burn patients and healthy volunteers, with 85% of them exhibiting a profound and persistent modulation over seven days. Interestingly, we showed that hydrocortisone enhanced the Shock-associated repression of adaptive, but also innate immunity. We found that the initial host response to Burn Shock encompasses wide and persistent modulation of gene expression, with profound modulation of pathways associated with metabolism and immunity. Importantly, hydrocortisone administration may worsen the immunosuppression associated with severe injury. These data should be taken into account in the risk ratio of hydrocortisone administration in patients with inflammatory Shock. ClinicalTrials.gov, NCT00149123 . Registered on 6 September 2005.
Karsten Knobloch - One of the best experts on this subject based on the ideXlab platform.
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insight in microcirculation and histomorphology during Burn Shock treatment using in vivo confocal laser scanning microscopy
Journal of Critical Care, 2010Co-Authors: M.a. Altintas, Ahmet Ali Altintas, Merlin Guggenheim, Matthias Aust, Andreas D. Niederbichler, Karsten Knobloch, Peter M. VogtAbstract:Abstract Purpose Microcirculatory disturbances are well known during Shock; however, the accompanied histomorphological alterations are widely unknown. We used high resolution confocal-laser-scanning microscopy for the evaluation of microcirculation and histomorphology during Burn Shock treatment. Methods Confocal-laser-scanning microscopy was performed in 10 Burn Shock patients (4 women, 6 men; aged 40.6 ± 11.4 years, Burn extent >20% body surface area) initially and 24 hours after Shock resuscitation. Ten matched hemodynamic stable Burn intensive care unit patients served as controls. The following parameters were evaluated: quantitative blood cell flow, cell size of the granular layer, basal layer thickness, and epidermal thickness. Results Quantitative blood cell flow in controls was 62.45 ± 3.39 cells per minute. Burn Shock significantly reduced blood cell flow to 37.27 ± 3.64 cells per minute; fluid resuscitation effectively restored baseline blood flow (65.18 ± 3.76 cells per minute) after 24 hours. Granular cell size was 793.61 ± 41.58 μ m 2 in controls vs 644.27 ± 42.96 μ m 2 during Burn Shock. Post resuscitation granular cell size measured 932.74 ± 38.83 μ m 2 . Basal layer thickness was 14.84 ± 0.59 μ m in controls, 13.26 ± 0.54 μ m in Burn patients at admission and before resuscitation, and 17.50 ± 0.46 μ m after resuscitation. Epidermal thickness in control patients was 49.60 ± 2.36 μ m, 37.83 ± 2.47 μ m in Burn patients at admission and 69.50 ± 3.18 μ m after resuscitation. Conclusions Confocal-laser-scanning microscopy provides a noninvasive tool for simultaneous evaluation of microcirculation and tissue histomorphology. It may help to assess the adequacy of and response to resuscitation of Burn patients early after trauma.
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Insight in microcirculation and histomorphology during Burn Shock treatment using in vivo confocal-laser-scanning microscopy ☆,☆☆
Journal of critical care, 2009Co-Authors: M.a. Altintas, Ahmet Ali Altintas, Merlin Guggenheim, Matthias Aust, Andreas D. Niederbichler, Karsten Knobloch, Peter M. VogtAbstract:Abstract Purpose Microcirculatory disturbances are well known during Shock; however, the accompanied histomorphological alterations are widely unknown. We used high resolution confocal-laser-scanning microscopy for the evaluation of microcirculation and histomorphology during Burn Shock treatment. Methods Confocal-laser-scanning microscopy was performed in 10 Burn Shock patients (4 women, 6 men; aged 40.6 ± 11.4 years, Burn extent >20% body surface area) initially and 24 hours after Shock resuscitation. Ten matched hemodynamic stable Burn intensive care unit patients served as controls. The following parameters were evaluated: quantitative blood cell flow, cell size of the granular layer, basal layer thickness, and epidermal thickness. Results Quantitative blood cell flow in controls was 62.45 ± 3.39 cells per minute. Burn Shock significantly reduced blood cell flow to 37.27 ± 3.64 cells per minute; fluid resuscitation effectively restored baseline blood flow (65.18 ± 3.76 cells per minute) after 24 hours. Granular cell size was 793.61 ± 41.58 μ m 2 in controls vs 644.27 ± 42.96 μ m 2 during Burn Shock. Post resuscitation granular cell size measured 932.74 ± 38.83 μ m 2 . Basal layer thickness was 14.84 ± 0.59 μ m in controls, 13.26 ± 0.54 μ m in Burn patients at admission and before resuscitation, and 17.50 ± 0.46 μ m after resuscitation. Epidermal thickness in control patients was 49.60 ± 2.36 μ m, 37.83 ± 2.47 μ m in Burn patients at admission and 69.50 ± 3.18 μ m after resuscitation. Conclusions Confocal-laser-scanning microscopy provides a noninvasive tool for simultaneous evaluation of microcirculation and tissue histomorphology. It may help to assess the adequacy of and response to resuscitation of Burn patients early after trauma.
Andreas D. Niederbichler - One of the best experts on this subject based on the ideXlab platform.
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insight in microcirculation and histomorphology during Burn Shock treatment using in vivo confocal laser scanning microscopy
Journal of Critical Care, 2010Co-Authors: M.a. Altintas, Ahmet Ali Altintas, Merlin Guggenheim, Matthias Aust, Andreas D. Niederbichler, Karsten Knobloch, Peter M. VogtAbstract:Abstract Purpose Microcirculatory disturbances are well known during Shock; however, the accompanied histomorphological alterations are widely unknown. We used high resolution confocal-laser-scanning microscopy for the evaluation of microcirculation and histomorphology during Burn Shock treatment. Methods Confocal-laser-scanning microscopy was performed in 10 Burn Shock patients (4 women, 6 men; aged 40.6 ± 11.4 years, Burn extent >20% body surface area) initially and 24 hours after Shock resuscitation. Ten matched hemodynamic stable Burn intensive care unit patients served as controls. The following parameters were evaluated: quantitative blood cell flow, cell size of the granular layer, basal layer thickness, and epidermal thickness. Results Quantitative blood cell flow in controls was 62.45 ± 3.39 cells per minute. Burn Shock significantly reduced blood cell flow to 37.27 ± 3.64 cells per minute; fluid resuscitation effectively restored baseline blood flow (65.18 ± 3.76 cells per minute) after 24 hours. Granular cell size was 793.61 ± 41.58 μ m 2 in controls vs 644.27 ± 42.96 μ m 2 during Burn Shock. Post resuscitation granular cell size measured 932.74 ± 38.83 μ m 2 . Basal layer thickness was 14.84 ± 0.59 μ m in controls, 13.26 ± 0.54 μ m in Burn patients at admission and before resuscitation, and 17.50 ± 0.46 μ m after resuscitation. Epidermal thickness in control patients was 49.60 ± 2.36 μ m, 37.83 ± 2.47 μ m in Burn patients at admission and 69.50 ± 3.18 μ m after resuscitation. Conclusions Confocal-laser-scanning microscopy provides a noninvasive tool for simultaneous evaluation of microcirculation and tissue histomorphology. It may help to assess the adequacy of and response to resuscitation of Burn patients early after trauma.
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Insight in microcirculation and histomorphology during Burn Shock treatment using in vivo confocal-laser-scanning microscopy ☆,☆☆
Journal of critical care, 2009Co-Authors: M.a. Altintas, Ahmet Ali Altintas, Merlin Guggenheim, Matthias Aust, Andreas D. Niederbichler, Karsten Knobloch, Peter M. VogtAbstract:Abstract Purpose Microcirculatory disturbances are well known during Shock; however, the accompanied histomorphological alterations are widely unknown. We used high resolution confocal-laser-scanning microscopy for the evaluation of microcirculation and histomorphology during Burn Shock treatment. Methods Confocal-laser-scanning microscopy was performed in 10 Burn Shock patients (4 women, 6 men; aged 40.6 ± 11.4 years, Burn extent >20% body surface area) initially and 24 hours after Shock resuscitation. Ten matched hemodynamic stable Burn intensive care unit patients served as controls. The following parameters were evaluated: quantitative blood cell flow, cell size of the granular layer, basal layer thickness, and epidermal thickness. Results Quantitative blood cell flow in controls was 62.45 ± 3.39 cells per minute. Burn Shock significantly reduced blood cell flow to 37.27 ± 3.64 cells per minute; fluid resuscitation effectively restored baseline blood flow (65.18 ± 3.76 cells per minute) after 24 hours. Granular cell size was 793.61 ± 41.58 μ m 2 in controls vs 644.27 ± 42.96 μ m 2 during Burn Shock. Post resuscitation granular cell size measured 932.74 ± 38.83 μ m 2 . Basal layer thickness was 14.84 ± 0.59 μ m in controls, 13.26 ± 0.54 μ m in Burn patients at admission and before resuscitation, and 17.50 ± 0.46 μ m after resuscitation. Epidermal thickness in control patients was 49.60 ± 2.36 μ m, 37.83 ± 2.47 μ m in Burn patients at admission and 69.50 ± 3.18 μ m after resuscitation. Conclusions Confocal-laser-scanning microscopy provides a noninvasive tool for simultaneous evaluation of microcirculation and tissue histomorphology. It may help to assess the adequacy of and response to resuscitation of Burn patients early after trauma.
