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Bursa of Fabricius

The Experts below are selected from a list of 213 Experts worldwide ranked by ideXlab platform

J A Vega – 1st expert on this subject based on the ideXlab platform

  • localization of trk neurotrophin receptor like proteins in avian primary lymphoid organs thymus and Bursa of Fabricius
    Journal of Neuroimmunology, 1996
    Co-Authors: E Ciriaco, R Laura, G Germanà, C Dallaglio, Jonas Hannestad, J J Huerta, J A Vega

    Abstract:

    Abstract The avian thymus and Bursa of Fabricius are the specific organs where the maturation and differentiation of T- and B-lymphocytes, respectively, take place. In the mammalian lymphoid organs mRNAs of the neurotrophins and their receptors have been identified but their localization at the protein level remains still unknown. This study was undertaken to analyze the localization of the Trk family of tyrosine kinase receptors in the avian primary lymphoid organs (thymus and Bursa of Fabricius) during the posthatching development using immunohistochemistry. These proteins serve as essential constituents of the high affinity receptors for neurotrophins. In the thymus of all groups of age specific immunoreactivity (IR) was observed for all three Trks: TrkA-like IR was found labelling medullary epithelial cells and a subpopulation of cortical epithelial cells; TrkB-like IR was found in the medullar dendritic cells and cortical macrophages; TrkC-like IR labelled the cortical epithelial cells and scattered medullar clusters of epithelial cells (including Hassal’s corpuscles). Quantitative analysis revealed age-dependent decrease in the area occupied by TrkA-like IR in the cortex, and age-dependent increase in the medulla; no changes were detected in the area occupied by TrkB-like IR; the TrkC-like immunoreactive cells increase from 7 to 30 days and then decrease. Regarding to the Bursa of Fabricius, TrkA- and TrkC-like IR were exclusively found in the epithelial cells of the follicle associated and the interfollicular epithelia, as well as TrkC-like IR in some medullary reticular epithelial cells of adult animals. Nevertheless, TrkB-like IR labelled extrafollicular unidentified cells in 7 days old animals, and the follicular secretory dendritic cells at 30 and 60 post-hatching. The area occupied by the medullary TrkB-like IR cells increased between 30 and 60 days. No immunostaining of lymphocytes was observed for any of the assessed antigens. The blood vessels of both the thymus and the Bursa of Fabricius were immunoreactive for TrkA- and TrkC-like proteins. The present results provide evidence for the localization of Trks in the non-lymphoid cells (epithelial and dendritic) of the avian primary lymphoid organs, suggesting a role for neurotrophins in these cells. Moreover, the selective cell localization of each Trk protein, and the absence of apparent overlapping, claims for a differential role of the specific Trk ligands. Whether or not these findings have functional relevance for T- and B-lymphocytes processing in avian primary lymphoid organs is discussed.

  • Muscarinic cholinergic receptor subtypes in the pigeon Bursa of Fabricius: a radioligand binding and autoradiographic study.
    Journal of neuroimmunology, 1996
    Co-Authors: A Ricci, E Ciriaco, E Bronzetti, L Felici, J A Vega, G Germanà

    Abstract:

    The pharmacological profile and the anatomical localisation of muscarinic cholinergic receptor subtypes were studied in the pigeon Bursa of Fabricius, using radioligand binding and autoradiographic techniques with [3H]quinuclidinyl benzilate (QNB) as a ligand. [3H]QNB was specifically bound to sections of Bursa of Fabricius. The binding was time-, temperature- and concentration-dependent. The dissociation constant was 0.31 +/- 0.02 nM, and the maximum density of binding sites averaged 38 +/- 2.5 fmol/mg protein. The pharmacological profile of [3H]QNB binding to sections of pigeon Bursa of Fabricius was consistent with the labelling of M2, M3 and M4 muscarinic receptor subtypes. Light microscope autoradiography showed the localisation of [3H]QNB binding sites in the medulla, in follicular septa, in the cortico-medullary border and in lesser amounts in the cortical layer. The functional significance of these receptors should be clarified in future studies.

E Ciriaco – 2nd expert on this subject based on the ideXlab platform

  • age related changes in the avian primary lymphoid organs thymus and Bursa of Fabricius
    Microscopy Research and Technique, 2003
    Co-Authors: E Ciriaco, Pablo Perez Pinera, Belen Diazesnal, R Laura

    Abstract:

    The avian primary lymphoid organs, the thymus and the Bursa of Fabricius, undergo age-dependent changes leading in some cases to the complete atrophy of the organ. Nevertheless, the timetable of the involutive process as well as the consequences in the structure and functionality of the organs vary largely in the time frame and structural changes among species. On the other hand, and in contrast with the large body of literature reporting the structural and functional changes in mammalian primary lymphoid organs, the age-dependent changes in avian thymus and Bursa of Fabricius are scarce, fragmentary, and heterogeneous. This article reviews the current literature on this topic, and focuses primarily on the involution of the Bursa of Fabricius. Microsc. Res. Tech. 62:482- 487, 2003. © 2003 Wiley-Liss, Inc.

  • localization of trk neurotrophin receptor like proteins in avian primary lymphoid organs thymus and Bursa of Fabricius
    Journal of Neuroimmunology, 1996
    Co-Authors: E Ciriaco, R Laura, G Germanà, C Dallaglio, Jonas Hannestad, J J Huerta, J A Vega

    Abstract:

    Abstract The avian thymus and Bursa of Fabricius are the specific organs where the maturation and differentiation of T- and B-lymphocytes, respectively, take place. In the mammalian lymphoid organs mRNAs of the neurotrophins and their receptors have been identified but their localization at the protein level remains still unknown. This study was undertaken to analyze the localization of the Trk family of tyrosine kinase receptors in the avian primary lymphoid organs (thymus and Bursa of Fabricius) during the posthatching development using immunohistochemistry. These proteins serve as essential constituents of the high affinity receptors for neurotrophins. In the thymus of all groups of age specific immunoreactivity (IR) was observed for all three Trks: TrkA-like IR was found labelling medullary epithelial cells and a subpopulation of cortical epithelial cells; TrkB-like IR was found in the medullar dendritic cells and cortical macrophages; TrkC-like IR labelled the cortical epithelial cells and scattered medullar clusters of epithelial cells (including Hassal’s corpuscles). Quantitative analysis revealed age-dependent decrease in the area occupied by TrkA-like IR in the cortex, and age-dependent increase in the medulla; no changes were detected in the area occupied by TrkB-like IR; the TrkC-like immunoreactive cells increase from 7 to 30 days and then decrease. Regarding to the Bursa of Fabricius, TrkA- and TrkC-like IR were exclusively found in the epithelial cells of the follicle associated and the interfollicular epithelia, as well as TrkC-like IR in some medullary reticular epithelial cells of adult animals. Nevertheless, TrkB-like IR labelled extrafollicular unidentified cells in 7 days old animals, and the follicular secretory dendritic cells at 30 and 60 post-hatching. The area occupied by the medullary TrkB-like IR cells increased between 30 and 60 days. No immunostaining of lymphocytes was observed for any of the assessed antigens. The blood vessels of both the thymus and the Bursa of Fabricius were immunoreactive for TrkA- and TrkC-like proteins. The present results provide evidence for the localization of Trks in the non-lymphoid cells (epithelial and dendritic) of the avian primary lymphoid organs, suggesting a role for neurotrophins in these cells. Moreover, the selective cell localization of each Trk protein, and the absence of apparent overlapping, claims for a differential role of the specific Trk ligands. Whether or not these findings have functional relevance for T- and B-lymphocytes processing in avian primary lymphoid organs is discussed.

  • Muscarinic cholinergic receptor subtypes in the pigeon Bursa of Fabricius: a radioligand binding and autoradiographic study.
    Journal of neuroimmunology, 1996
    Co-Authors: A Ricci, E Ciriaco, E Bronzetti, L Felici, J A Vega, G Germanà

    Abstract:

    The pharmacological profile and the anatomical localisation of muscarinic cholinergic receptor subtypes were studied in the pigeon Bursa of Fabricius, using radioligand binding and autoradiographic techniques with [3H]quinuclidinyl benzilate (QNB) as a ligand. [3H]QNB was specifically bound to sections of Bursa of Fabricius. The binding was time-, temperature- and concentration-dependent. The dissociation constant was 0.31 +/- 0.02 nM, and the maximum density of binding sites averaged 38 +/- 2.5 fmol/mg protein. The pharmacological profile of [3H]QNB binding to sections of pigeon Bursa of Fabricius was consistent with the labelling of M2, M3 and M4 muscarinic receptor subtypes. Light microscope autoradiography showed the localisation of [3H]QNB binding sites in the medulla, in follicular septa, in the cortico-medullary border and in lesser amounts in the cortical layer. The functional significance of these receptors should be clarified in future studies.

Yan-fei Li – 3rd expert on this subject based on the ideXlab platform

  • Effects of Sub-Chronic Aluminum Intoxication on Growth and Morphological Structure of Spleen and Bursa of Fabricius in Chickens
    2009 3rd International Conference on Bioinformatics and Biomedical Engineering, 2009
    Co-Authors: Chong-wei Hu, Yan-fei Li

    Abstract:

    The model of different level’s sub-chronic aluminum intoxication in chickens was established by continuous peritoneal injection different gradient aluminum trichloride (AICI3). The growth index of spleen and Bursa of Fabricius were detected and the structures of them were observed by optical microscope and transmission electron microscope. The results showed that (1) the growth index of spleen and Bursa of Fabricius of aluminum toxical group was significant lower than the control group in chickens (P

  • Effects of Sub-Chronic Aluminum Intoxication on Growth and Morphological Structure of Spleen and Bursa of Fabricius in Chickens
    2009 3rd International Conference on Bioinformatics and Biomedical Engineering, 2009
    Co-Authors: Chong-wei Hu, Yan-fei Li

    Abstract:

    The model of different level’s sub-chronic aluminum intoxication in chickens was established by continuous peritoneal injection different gradient aluminum trichloride (AICI 3 ). The growth index of spleen and Bursa of Fabricius were detected and the structures of them were observed by optical microscope and transmission electron microscope. The results showed that (1) the growth index of spleen and Bursa of Fabricius of aluminum toxical group was significant lower than the control group in chickens (P 3 . (2) In fact of histopathologically, middle and low dose groups had no apparently pathological changes. Spleen presented changes of apoptotic morphology and germinal center of Bursal nodule hyperplasyed in high dose group. (3) In the fact of ultrastructurally, low dose group had no apparently pathological changes. In middle and high dose groups, karyolemma broken, mitochondria swelled, cristae fragmentated or disappeared, rough endoplasmic reticulum dilated, cisternas widen, confluenced vacuole and presented typical mark shape of apoptosis in lymphocyte of spleen; karyolemma broken, caryolysised, myelin bodys appeared, the number of organelles within intracytoplasm decreased significantly in lymphocyte of Bursa of Fabricius. The results indicated that aluminum intoxication greatly hindered the development of spleen and Bursa of Fabricius, as a consequence obvious pathological injury of spleen and Bursa of Fabricius.