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Yih-ming Yang - One of the best experts on this subject based on the ideXlab platform.
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relationship of Burst Forming Unit erythroid progenitors and their dna synthesis stage to fetal hemoglobin levels in hydroxyurea treated patients with sickle cell anemia
American Journal of Hematology, 1994Co-Authors: Vipul N. Mankad, Surendra Baliga, Karen Phillips, Arvind K. Shah, Yih-ming YangAbstract:DNA-synthesis stage and total number of circulating Burst-Forming-Units-erythroid (BFU-E) have been inversely correlated with hemoglobin F levels in the peripheral blood, as well as in the cells from the BFU-E-derived colonies obtained from homozygous sickle cell anemia (SS) patients during steady state. Similar studies in SS patients treated with cytotoxic agents have not been reported. However, regeneration of the erythroid marrow that follows the cytoreduction phase of chemotherapy has been suggested as one of the mechanisms of stimulation of fetal hemoglobin synthesis. Therefore, a longitudinal study of hemopoiesis in hydroxyurea-treated SS patients was conducted. Thirty-two sets of hemopoietic studies, including total circulating BFU-E and S-phase BFU-E, were obtained from three patients treated with hydroxyurea. A dose-dependent decrease in total BFU-E colonies occurred in peripheral blood of all three patients (r = -0.58, -0.85, and -0.97, respectively, with each P < 0.05). There was a strong positive correlation between hydroxyurea dose and fetal hemoglobin levels in two of the three patients who responded clinically (r = 0.89618 and 0.88632, respectively, with each P < 0.01). When data from all patients were combined (n = 32), there was a strong, inverse, linear relationship between total number of BFU-E and percentage S-phase BFU-E with fetal hemoglobin levels (r = -0.6649 and -0.7404, respectively, with each P < 0.0001). A stronger, curvilinear, multiple relationship was detected between total BFU-E and percentage S-phase BFU-E with fetal hemoglobin levels (R = 0.8351 and 0.8602 with each P < 0.0001).
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Relationship of Burst-Forming-Unit-erythroid progenitors and their DNA-synthesis stage to fetal hemoglobin levels in hydroxyurea-treated patients with sickle cell anemia.
American journal of hematology, 1994Co-Authors: Vipul N. Mankad, Surendra Baliga, Karen Phillips, Arvind K. Shah, Yih-ming YangAbstract:DNA-synthesis stage and total number of circulating Burst-Forming-Units-erythroid (BFU-E) have been inversely correlated with hemoglobin F levels in the peripheral blood, as well as in the cells from the BFU-E-derived colonies obtained from homozygous sickle cell anemia (SS) patients during steady state. Similar studies in SS patients treated with cytotoxic agents have not been reported. However, regeneration of the erythroid marrow that follows the cytoreduction phase of chemotherapy has been suggested as one of the mechanisms of stimulation of fetal hemoglobin synthesis. Therefore, a longitudinal study of hemopoiesis in hydroxyurea-treated SS patients was conducted. Thirty-two sets of hemopoietic studies, including total circulating BFU-E and S-phase BFU-E, were obtained from three patients treated with hydroxyurea. A dose-dependent decrease in total BFU-E colonies occurred in peripheral blood of all three patients (r = −0.58, −0.85, and −0.97, respectively, with each P < 0.05). There was a strong positive correlation between hydroxyurea dose and fetal hemoglobin levels in two of the three patients who responded clinically (r = 0.89618 and 0.88632, respectively, with each P < 0.01). When data from all patients were combined (n = 32), there was a strong, inverse, linear relationship between total number of BFU-E and percentage S-phase BFU-E with fetal hemoglobin levels (r = −0.6649 and −0.7404, respectively, with each P < 0.0001). A stronger, curvilinear, multiple relationship was detected between total BFU-E and percentage S-phase BFU-E with fetal hemoglobin levels (R = 0.8351 and 0.8602 with each P < 0.0001). © 1994 Wiley-Liss, Inc.
Keishi Abe - One of the best experts on this subject based on the ideXlab platform.
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Effects of thyroid hormone on carbonic anhydrase I concentration in human erythroid Burst-Forming Unit-derived cells.
Endocrinology, 1996Co-Authors: Noriyo Sayama, Katsumi Yoshida, K Endo, Yoshinori Kiso, Hiroshi Fukazawa, Kouki Mori, Kyou Kikuchi, Yoshihiko Aizawa, Hironobu Hori, Keishi AbeAbstract:Individuals with hyperthyroidism exhibit red blood cell concentrations of carbonic anhydrase I (CAI) that reflect the integrated serum thyroid hormone concentration over the preceding few months. Furthermore, T3, at a physiological free concentration, decreases the CAI concentration in human erythroleukemic YN-1 cells. The effect of T3 on CAI concentration in Burst-Forming Unit-erythroid (BFU-E)- derived cells, obtained by culturing peripheral blood mononuclear cells with various cytokines, including erythropoietin, has now been investigated. BFU-E-derived cells contained a high concentration of CAI (mean +/- SE, 4.8 +/- 0.8 x 10(-12) mol/10(6) cells; n = 8). The CAI in BFU-E-derived cells was immunologically identical to that present in mature red blood cells. T3 decreased the CAI concentration in BFU-E-derived cells in a dose-dependent manner (28%, 47% and 75% decreases at 3 x 10(-10), 1 x 10(-9), and 3 x 10(-9) mol/liter T3, respectively). These results suggest that BFU-E-derived cells may be used to s...
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effects of thyroid hormone on carbonic anhydrase i concentration in human erythroid Burst Forming Unit derived cells
Endocrinology, 1996Co-Authors: Noriyo Sayama, Katsumi Yoshida, K Endo, Yoshinori Kiso, Hiroshi Fukazawa, Kouki Mori, Kyou Kikuchi, Yoshihiko Aizawa, Hironobu Hori, Keishi AbeAbstract:Individuals with hyperthyroidism exhibit red blood cell concentrations of carbonic anhydrase I (CAI) that reflect the integrated serum thyroid hormone concentration over the preceding few months. Furthermore, T3, at a physiological free concentration, decreases the CAI concentration in human erythroleukemic YN-1 cells. The effect of T3 on CAI concentration in Burst-Forming Unit-erythroid (BFU-E)- derived cells, obtained by culturing peripheral blood mononuclear cells with various cytokines, including erythropoietin, has now been investigated. BFU-E-derived cells contained a high concentration of CAI (mean +/- SE, 4.8 +/- 0.8 x 10(-12) mol/10(6) cells; n = 8). The CAI in BFU-E-derived cells was immunologically identical to that present in mature red blood cells. T3 decreased the CAI concentration in BFU-E-derived cells in a dose-dependent manner (28%, 47% and 75% decreases at 3 x 10(-10), 1 x 10(-9), and 3 x 10(-9) mol/liter T3, respectively). These results suggest that BFU-E-derived cells may be used to study the effect of T3 on human red blood cell CAI. This system may prove useful in the tissue diagnosis of resistance to thyroid hormone.
Riitta Kekomäki - One of the best experts on this subject based on the ideXlab platform.
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loss of the ability to generate large Burst Forming Unit like megakaryocytic colonies from thawed cord blood in semisolid cultures after short term suspension culture
Vox Sanguinis, 2015Co-Authors: Mikko Eskola, Sari Bäckman, Sari Möttönen, Riitta KekomäkiAbstract:Background and Objectives Total colony-Forming cells from thawed cord blood Units (CBUs) include megakaryocytic colony-Forming Units (CFU-Mks), which survive the freezing process. The aim of this study was to evaluate whether different megakaryocytic progenitors from unseparated CBUs survive the freezing process and a short-term liquid culture. Materials and Methods Thawed samples of CBUs were cultured in liquid medium. During the cultures, serial samples were drawn to assess the growth of different megakaryocytic progenitors in a semisolid collagen medium with identical cytokines as in the liquid medium. Megakaryocytic cells were detected using immunohistochemistry and flow cytometry. Results In suspension culture, the megakaryocytic progenitors almost completely lost the ability to generate large (Burst-Forming Unit-like, BFU-like) megakaryocytic colonies in semisolid cultures (large colonies, median count per chamber d0: 7·25 vs. d7: 1·5; P < 0·0001), whereas the number of small colonies (median count per chamber d0: 7·25 vs. d7: 16·0; P = 0·0505) peaked at day seven. Further 7-day culture in suspension resulted in the decline of small colonies as well (d7: 16·0 vs. d14: 5·75; P = 0·0088). Total CFU-Mk count declined from 23·3 (range 12·5–34·0) at d0 to 7·25 (range 1·0–13·5) at d14 (P < 0·0001). Conclusion Immediately post-thaw, CBUs possess an ability to generate large BFU-like megakaryocytic colonies, whereas the colonies were not detectable in most CBUs in semisolid culture after a short suspension culture. Small CFU-Mks were observed throughout the cultures. It may be that the BFU-Mk colonies matured and acquired CFU-Mk behaviour.
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Loss of the ability to generate large Burst-Forming Unit-like megakaryocytic colonies from thawed cord blood in semisolid cultures after short term suspension culture.
Vox sanguinis, 2014Co-Authors: Mikko Eskola, Sari Bäckman, Sari Möttönen, Riitta KekomäkiAbstract:Background and Objectives Total colony-Forming cells from thawed cord blood Units (CBUs) include megakaryocytic colony-Forming Units (CFU-Mks), which survive the freezing process. The aim of this study was to evaluate whether different megakaryocytic progenitors from unseparated CBUs survive the freezing process and a short-term liquid culture. Materials and Methods Thawed samples of CBUs were cultured in liquid medium. During the cultures, serial samples were drawn to assess the growth of different megakaryocytic progenitors in a semisolid collagen medium with identical cytokines as in the liquid medium. Megakaryocytic cells were detected using immunohistochemistry and flow cytometry. Results In suspension culture, the megakaryocytic progenitors almost completely lost the ability to generate large (Burst-Forming Unit-like, BFU-like) megakaryocytic colonies in semisolid cultures (large colonies, median count per chamber d0: 7·25 vs. d7: 1·5; P
Vipul N. Mankad - One of the best experts on this subject based on the ideXlab platform.
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relationship of Burst Forming Unit erythroid progenitors and their dna synthesis stage to fetal hemoglobin levels in hydroxyurea treated patients with sickle cell anemia
American Journal of Hematology, 1994Co-Authors: Vipul N. Mankad, Surendra Baliga, Karen Phillips, Arvind K. Shah, Yih-ming YangAbstract:DNA-synthesis stage and total number of circulating Burst-Forming-Units-erythroid (BFU-E) have been inversely correlated with hemoglobin F levels in the peripheral blood, as well as in the cells from the BFU-E-derived colonies obtained from homozygous sickle cell anemia (SS) patients during steady state. Similar studies in SS patients treated with cytotoxic agents have not been reported. However, regeneration of the erythroid marrow that follows the cytoreduction phase of chemotherapy has been suggested as one of the mechanisms of stimulation of fetal hemoglobin synthesis. Therefore, a longitudinal study of hemopoiesis in hydroxyurea-treated SS patients was conducted. Thirty-two sets of hemopoietic studies, including total circulating BFU-E and S-phase BFU-E, were obtained from three patients treated with hydroxyurea. A dose-dependent decrease in total BFU-E colonies occurred in peripheral blood of all three patients (r = -0.58, -0.85, and -0.97, respectively, with each P < 0.05). There was a strong positive correlation between hydroxyurea dose and fetal hemoglobin levels in two of the three patients who responded clinically (r = 0.89618 and 0.88632, respectively, with each P < 0.01). When data from all patients were combined (n = 32), there was a strong, inverse, linear relationship between total number of BFU-E and percentage S-phase BFU-E with fetal hemoglobin levels (r = -0.6649 and -0.7404, respectively, with each P < 0.0001). A stronger, curvilinear, multiple relationship was detected between total BFU-E and percentage S-phase BFU-E with fetal hemoglobin levels (R = 0.8351 and 0.8602 with each P < 0.0001).
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Relationship of Burst-Forming-Unit-erythroid progenitors and their DNA-synthesis stage to fetal hemoglobin levels in hydroxyurea-treated patients with sickle cell anemia.
American journal of hematology, 1994Co-Authors: Vipul N. Mankad, Surendra Baliga, Karen Phillips, Arvind K. Shah, Yih-ming YangAbstract:DNA-synthesis stage and total number of circulating Burst-Forming-Units-erythroid (BFU-E) have been inversely correlated with hemoglobin F levels in the peripheral blood, as well as in the cells from the BFU-E-derived colonies obtained from homozygous sickle cell anemia (SS) patients during steady state. Similar studies in SS patients treated with cytotoxic agents have not been reported. However, regeneration of the erythroid marrow that follows the cytoreduction phase of chemotherapy has been suggested as one of the mechanisms of stimulation of fetal hemoglobin synthesis. Therefore, a longitudinal study of hemopoiesis in hydroxyurea-treated SS patients was conducted. Thirty-two sets of hemopoietic studies, including total circulating BFU-E and S-phase BFU-E, were obtained from three patients treated with hydroxyurea. A dose-dependent decrease in total BFU-E colonies occurred in peripheral blood of all three patients (r = −0.58, −0.85, and −0.97, respectively, with each P < 0.05). There was a strong positive correlation between hydroxyurea dose and fetal hemoglobin levels in two of the three patients who responded clinically (r = 0.89618 and 0.88632, respectively, with each P < 0.01). When data from all patients were combined (n = 32), there was a strong, inverse, linear relationship between total number of BFU-E and percentage S-phase BFU-E with fetal hemoglobin levels (r = −0.6649 and −0.7404, respectively, with each P < 0.0001). A stronger, curvilinear, multiple relationship was detected between total BFU-E and percentage S-phase BFU-E with fetal hemoglobin levels (R = 0.8351 and 0.8602 with each P < 0.0001). © 1994 Wiley-Liss, Inc.
Noriyo Sayama - One of the best experts on this subject based on the ideXlab platform.
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Effects of thyroid hormone on carbonic anhydrase I concentration in human erythroid Burst-Forming Unit-derived cells.
Endocrinology, 1996Co-Authors: Noriyo Sayama, Katsumi Yoshida, K Endo, Yoshinori Kiso, Hiroshi Fukazawa, Kouki Mori, Kyou Kikuchi, Yoshihiko Aizawa, Hironobu Hori, Keishi AbeAbstract:Individuals with hyperthyroidism exhibit red blood cell concentrations of carbonic anhydrase I (CAI) that reflect the integrated serum thyroid hormone concentration over the preceding few months. Furthermore, T3, at a physiological free concentration, decreases the CAI concentration in human erythroleukemic YN-1 cells. The effect of T3 on CAI concentration in Burst-Forming Unit-erythroid (BFU-E)- derived cells, obtained by culturing peripheral blood mononuclear cells with various cytokines, including erythropoietin, has now been investigated. BFU-E-derived cells contained a high concentration of CAI (mean +/- SE, 4.8 +/- 0.8 x 10(-12) mol/10(6) cells; n = 8). The CAI in BFU-E-derived cells was immunologically identical to that present in mature red blood cells. T3 decreased the CAI concentration in BFU-E-derived cells in a dose-dependent manner (28%, 47% and 75% decreases at 3 x 10(-10), 1 x 10(-9), and 3 x 10(-9) mol/liter T3, respectively). These results suggest that BFU-E-derived cells may be used to s...
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effects of thyroid hormone on carbonic anhydrase i concentration in human erythroid Burst Forming Unit derived cells
Endocrinology, 1996Co-Authors: Noriyo Sayama, Katsumi Yoshida, K Endo, Yoshinori Kiso, Hiroshi Fukazawa, Kouki Mori, Kyou Kikuchi, Yoshihiko Aizawa, Hironobu Hori, Keishi AbeAbstract:Individuals with hyperthyroidism exhibit red blood cell concentrations of carbonic anhydrase I (CAI) that reflect the integrated serum thyroid hormone concentration over the preceding few months. Furthermore, T3, at a physiological free concentration, decreases the CAI concentration in human erythroleukemic YN-1 cells. The effect of T3 on CAI concentration in Burst-Forming Unit-erythroid (BFU-E)- derived cells, obtained by culturing peripheral blood mononuclear cells with various cytokines, including erythropoietin, has now been investigated. BFU-E-derived cells contained a high concentration of CAI (mean +/- SE, 4.8 +/- 0.8 x 10(-12) mol/10(6) cells; n = 8). The CAI in BFU-E-derived cells was immunologically identical to that present in mature red blood cells. T3 decreased the CAI concentration in BFU-E-derived cells in a dose-dependent manner (28%, 47% and 75% decreases at 3 x 10(-10), 1 x 10(-9), and 3 x 10(-9) mol/liter T3, respectively). These results suggest that BFU-E-derived cells may be used to study the effect of T3 on human red blood cell CAI. This system may prove useful in the tissue diagnosis of resistance to thyroid hormone.
