The Experts below are selected from a list of 2172 Experts worldwide ranked by ideXlab platform
Anne Belinda Bjerre - One of the best experts on this subject based on the ideXlab platform.
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Butanol Fermentation of the brown seaweed laminaria digitata by clostridium beijerinckii dsm 6422
Bioresource Technology, 2017Co-Authors: Xiaoru Hou, Irini Angelidaki, Nikolaj From, W J J Huijgen, Anne Belinda BjerreAbstract:Seaweed represents an abundant, renewable, and fast-growing biomass resource for 3rd generation biofuel production. This study reports an efficient Butanol Fermentation Process carried out by Clostridium beijerinckii DSM-6422 using enzymatic hydrolysate of the sugar-rich brown seaweed Laminaria digitata harvested from the coast of the Danish North Sea as substrate. The highest Butanol yield (0.42g/g-consumed-substrates) compared to literature was achieved, with a significantly higher Butanol:acetone-Butanol-ethanol (ABE) molar ratio (0.85) than typical (0.6). This demonstrates the possibility of using the seaweed L. digitata as a potential biomass for Butanol production. For the first time, consumption of alginate components was observed by C. beijerinckii DSM-6422. The efficient utilization of sugars and lactic acid further highlighted the potential of using this strain for future development of large-scale cost-effective Butanol production based on (ensiled) seaweed.
Mustafa Kansiz - One of the best experts on this subject based on the ideXlab platform.
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mid infrared spectroscopy coupled to sequential injection analysis for the on line monitoring of the acetone Butanol Fermentation Process
Analytica Chimica Acta, 2001Co-Authors: Mustafa Kansiz, Richard J Gapes, Donald Mcnaughton, Bernhard Lendl, Christian K SchusterAbstract:Fourier transform infrared (FTIR) spectroscopy, coupled to sequential injection analysis (SIA) was employed for the on-line monitoring of an acetone–Butanol Fermentation by simultaneously determining acetone, acetate, n-Butanol, butyrate and glucose from the mid-IR spectra of the samples. The analysis system (SIA-FTIR) developed was completely computer controlled, requiring only minute amounts of NaOH and Na2CO3 as reagents and was capable of performing 30 analyte determinations per hour. From a batch Fermentation run of 55 h duration, samples were automatically drawn and analysed at 14 different points of time. The analysis by SIA-FTIR comprised of triplicate injections, from each of which two spectra one with 4 cm −1 the other with 8 cm −1 spectral resolution were obtained. In addition, triplicate reference analysis by gas chromatography and enzymatic test kits were also done at corresponding points of time. Based on the data of the reference analysis a partial least squares (PLS) model was established and validated by a leave-one-out cross validation. Best results were obtained using the second derivatised spectra collected at 8 cm −1 resolution. The regression coefficients and standard errors of prediction (S.E.P.) were as follows: acetone, r = 0.999, S.E.P. = 0.077 g/l; acetate, r = 0.998, S.E.P. = 0.063 g/l; butyrate, r = 0.955 and S.E.P. = 0.058 g/l; n-Butanol, r = 0.999, S.E.P. = 0.301 g/l and glucose, r = 0.999, S.E.P. = 0.493 g/l. The high quality of the results obtained, and the observation that the precision of the SIA-FTIR analysis was as good and often better than those of the reference methods, showed that SIA-FTIR is a powerful tool for rapid on-line bioProcess monitoring. © 2001 Elsevier Science B.V. All rights reserved.
B. Saha - One of the best experts on this subject based on the ideXlab platform.
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Cellulosic Butanol (ABE) Biofuel Production from Sweet Sorghum Bagasse (SSB): Impact of Hot Water Pretreatment and Solid Loadings on Fermentation Employing Clostridium beijerinckii P260
BioEnergy Research, 2016Co-Authors: N. Qureshi, S. Liu, S. Hughes, D. Palmquist, B. Dien, B. SahaAbstract:A novel Butanol Fermentation Process was developed in which sweet sorghum bagasse (SSB) was pretreated using liquid hot water (LHW) pretreatment technique followed by enzymatic hydrolysis and Butanol (acetone Butanol ethanol (ABE)) Fermentation. A pretreatment temperature of 200 °C resulted in the generation of a hydrolyzate that inhibited Butanol Fermentation. When SSB pretreatment temperature was decreased to 190 °C (0-min holding time), the hydrolyzate was successfully fermented without inhibition and an ABE productivity of 0.51 g L^−1 h^−1 was achieved which is comparable to the 0.49 g L^−1 h^−1 observed in the control Fermentation where glucose was used as a feedstock. These results are based on the use of 86 g L^−1 SSB solid loadings in the pretreatment reactors. We were also able to increase SSB solid loadings from 120 to 200 g L^−1 in the pretreatment step (190 °C) followed by hydrolysis and Butanol Fermentation. As pretreatment solid loadings increased, ABE yield remained in the range of 0.38–0.46. In these studies, a maximum ABE concentration of 16.88 g L^−1 was achieved. Using the LHW pretreatment technique, 88.40–96.00 % of polymeric sugars (cellulose + hemicellulose) were released in the SSB hydrolyzate. The LHW pretreatment technique does not require chemical additions and is environmentally friendly, and the hydrolyzate can be used successfully for Butanol Fermentation.
Christian K Schuster - One of the best experts on this subject based on the ideXlab platform.
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mid infrared spectroscopy coupled to sequential injection analysis for the on line monitoring of the acetone Butanol Fermentation Process
Analytica Chimica Acta, 2001Co-Authors: Mustafa Kansiz, Richard J Gapes, Donald Mcnaughton, Bernhard Lendl, Christian K SchusterAbstract:Fourier transform infrared (FTIR) spectroscopy, coupled to sequential injection analysis (SIA) was employed for the on-line monitoring of an acetone–Butanol Fermentation by simultaneously determining acetone, acetate, n-Butanol, butyrate and glucose from the mid-IR spectra of the samples. The analysis system (SIA-FTIR) developed was completely computer controlled, requiring only minute amounts of NaOH and Na2CO3 as reagents and was capable of performing 30 analyte determinations per hour. From a batch Fermentation run of 55 h duration, samples were automatically drawn and analysed at 14 different points of time. The analysis by SIA-FTIR comprised of triplicate injections, from each of which two spectra one with 4 cm −1 the other with 8 cm −1 spectral resolution were obtained. In addition, triplicate reference analysis by gas chromatography and enzymatic test kits were also done at corresponding points of time. Based on the data of the reference analysis a partial least squares (PLS) model was established and validated by a leave-one-out cross validation. Best results were obtained using the second derivatised spectra collected at 8 cm −1 resolution. The regression coefficients and standard errors of prediction (S.E.P.) were as follows: acetone, r = 0.999, S.E.P. = 0.077 g/l; acetate, r = 0.998, S.E.P. = 0.063 g/l; butyrate, r = 0.955 and S.E.P. = 0.058 g/l; n-Butanol, r = 0.999, S.E.P. = 0.301 g/l and glucose, r = 0.999, S.E.P. = 0.493 g/l. The high quality of the results obtained, and the observation that the precision of the SIA-FTIR analysis was as good and often better than those of the reference methods, showed that SIA-FTIR is a powerful tool for rapid on-line bioProcess monitoring. © 2001 Elsevier Science B.V. All rights reserved.
Xiaoru Hou - One of the best experts on this subject based on the ideXlab platform.
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Butanol Fermentation of the brown seaweed laminaria digitata by clostridium beijerinckii dsm 6422
Bioresource Technology, 2017Co-Authors: Xiaoru Hou, Irini Angelidaki, Nikolaj From, W J J Huijgen, Anne Belinda BjerreAbstract:Seaweed represents an abundant, renewable, and fast-growing biomass resource for 3rd generation biofuel production. This study reports an efficient Butanol Fermentation Process carried out by Clostridium beijerinckii DSM-6422 using enzymatic hydrolysate of the sugar-rich brown seaweed Laminaria digitata harvested from the coast of the Danish North Sea as substrate. The highest Butanol yield (0.42g/g-consumed-substrates) compared to literature was achieved, with a significantly higher Butanol:acetone-Butanol-ethanol (ABE) molar ratio (0.85) than typical (0.6). This demonstrates the possibility of using the seaweed L. digitata as a potential biomass for Butanol production. For the first time, consumption of alginate components was observed by C. beijerinckii DSM-6422. The efficient utilization of sugars and lactic acid further highlighted the potential of using this strain for future development of large-scale cost-effective Butanol production based on (ensiled) seaweed.
