The Experts below are selected from a list of 297 Experts worldwide ranked by ideXlab platform

Leroy B. Townsend - One of the best experts on this subject based on the ideXlab platform.

Yufen Zhao - One of the best experts on this subject based on the ideXlab platform.

Marie E. Migaud - One of the best experts on this subject based on the ideXlab platform.

Kristjan Gudmundsson - One of the best experts on this subject based on the ideXlab platform.

Marc M. Greenberg - One of the best experts on this subject based on the ideXlab platform.

  • Repair of DNA Containing Fapy·dG and its β-C-nuCleoside analogue by formamidopyrimidine DNA glyCosylase and MutY
    Biochemistry, 2003
    Co-Authors: Carissa J. Wiederholt, Michael O. Delaney, Mary Ann Pope, Sheila S. David, Marc M. Greenberg
    Abstract:

    Fapy·dG is produCed in DNA as a result of oxidative stress. Under some Conditions Fapy·dG is formed in greater yields than 8-oxodG from a Common ChemiCal preCursor. ReCently, Fapy·dG and its C-nuCleoside analogue were inCorporated in ChemiCally synthesized oligonuCleotides at defined sites. Like 8-oxodG, Fapy·dG instruCts DNA polymerase to misinCorporate dA opposite it in vitro. The interaCtions of DNA Containing Fapy·dG or the nonhydrolyzable analogue with Fpg and MutY are desCribed. Fpg exCises Fapy·dG (KM = 2.0 nM, kCat = 0.14 min-1) opposite dC ∼17-fold more effiCiently than when mispaired with dA, whiCh is misinserted by DNA polymerase in vitro. Fpg also prefers to bind duplexes Containing Fapy·dG·dC or β-C-Fapy·dG·dC Compared to those in whiCh the lesion is opposite dA. MutY inCises dA when it is opposite Fapy·dG and strongly binds duplexes Containing the lesion or β-C-Fapy·dG. InCision from Fapy·dG·dA is faster than from dG·dA mispairs but slower than from DNA Containing 8-oxodG opposite dA. These ...

  • repair of dna Containing fapy dg and its β C nuCleoside analogue by formamidopyrimidine dna glyCosylase and muty
    Biochemistry, 2003
    Co-Authors: Carissa J. Wiederholt, Michael O. Delaney, Mary Ann Pope, Sheila S. David, Marc M. Greenberg
    Abstract:

    Fapy·dG is produCed in DNA as a result of oxidative stress. Under some Conditions Fapy·dG is formed in greater yields than 8-oxodG from a Common ChemiCal preCursor. ReCently, Fapy·dG and its C-nuCleoside analogue were inCorporated in ChemiCally synthesized oligonuCleotides at defined sites. Like 8-oxodG, Fapy·dG instruCts DNA polymerase to misinCorporate dA opposite it in vitro. The interaCtions of DNA Containing Fapy·dG or the nonhydrolyzable analogue with Fpg and MutY are desCribed. Fpg exCises Fapy·dG (KM = 2.0 nM, kCat = 0.14 min-1) opposite dC ∼17-fold more effiCiently than when mispaired with dA, whiCh is misinserted by DNA polymerase in vitro. Fpg also prefers to bind duplexes Containing Fapy·dG·dC or β-C-Fapy·dG·dC Compared to those in whiCh the lesion is opposite dA. MutY inCises dA when it is opposite Fapy·dG and strongly binds duplexes Containing the lesion or β-C-Fapy·dG. InCision from Fapy·dG·dA is faster than from dG·dA mispairs but slower than from DNA Containing 8-oxodG opposite dA. These ...