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Abdelazim Elsayed Elhelaly - One of the best experts on this subject based on the ideXlab platform.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 Cells and protection using rosmarinic and ascorbic a
Food Chemistry, 2019Co-Authors: Abdelazim Elsayed Elhelaly, Waleed R Elghareeb, Hitoshi Chiba, Wageh Sobhy DarwishAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (Caco-2) Cell Line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed Caco-2 Cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected Caco-2 Cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 Cells and protection using rosmarinic and ascorbic acids
Food Chemistry, 2019Co-Authors: Abdelazim Elsayed Elhelaly, Waleed R Elghareeb, Hitoshi Chiba, Wageh Sobhy DarwishAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (Caco-2) Cell Line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed Caco-2 Cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected Caco-2 Cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
Wageh Sobhy Darwish - One of the best experts on this subject based on the ideXlab platform.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 Cells and protection using rosmarinic and ascorbic a
Food Chemistry, 2019Co-Authors: Abdelazim Elsayed Elhelaly, Waleed R Elghareeb, Hitoshi Chiba, Wageh Sobhy DarwishAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (Caco-2) Cell Line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed Caco-2 Cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected Caco-2 Cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 Cells and protection using rosmarinic and ascorbic acids
Food Chemistry, 2019Co-Authors: Abdelazim Elsayed Elhelaly, Waleed R Elghareeb, Hitoshi Chiba, Wageh Sobhy DarwishAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (Caco-2) Cell Line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed Caco-2 Cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected Caco-2 Cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
Waleed R Elghareeb - One of the best experts on this subject based on the ideXlab platform.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 Cells and protection using rosmarinic and ascorbic a
Food Chemistry, 2019Co-Authors: Abdelazim Elsayed Elhelaly, Waleed R Elghareeb, Hitoshi Chiba, Wageh Sobhy DarwishAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (Caco-2) Cell Line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed Caco-2 Cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected Caco-2 Cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 Cells and protection using rosmarinic and ascorbic acids
Food Chemistry, 2019Co-Authors: Abdelazim Elsayed Elhelaly, Waleed R Elghareeb, Hitoshi Chiba, Wageh Sobhy DarwishAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (Caco-2) Cell Line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed Caco-2 Cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected Caco-2 Cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
Hitoshi Chiba - One of the best experts on this subject based on the ideXlab platform.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 Cells and protection using rosmarinic and ascorbic a
Food Chemistry, 2019Co-Authors: Abdelazim Elsayed Elhelaly, Waleed R Elghareeb, Hitoshi Chiba, Wageh Sobhy DarwishAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (Caco-2) Cell Line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed Caco-2 Cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected Caco-2 Cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 Cells and protection using rosmarinic and ascorbic acids
Food Chemistry, 2019Co-Authors: Abdelazim Elsayed Elhelaly, Waleed R Elghareeb, Hitoshi Chiba, Wageh Sobhy DarwishAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (Caco-2) Cell Line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed Caco-2 Cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected Caco-2 Cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
Dinoraz Velez - One of the best experts on this subject based on the ideXlab platform.
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intestinal transport of cylindrospermopsin using the caco 2 Cell Line
Toxicology in Vitro, 2017Co-Authors: Silvia Pichardo, Vicenta Devesa, Dinoraz Velez, Maria Puerto, Ana M CameanAbstract:Cylindrospermopsin (CYN) is a cyanotoxin produced by various cyanobacterial species. It is a water soluble zwitterion, stable at extreme temperatures and pH. Despite the main route of exposure to CYN is through drinking water and food, there is a lack of data concerning its intestinal absorption and the mechanisms implicated. The aim of this study was to characterize the mechanisms involved in the intestinal absorption of CYN, using Caco-2 human Cell Line as a model of the intestinal epithelium. The results obtained in the present work increases the limited knowledge regarding CYN transport across the intestinal epithelium and identifies the paraCellular route as an important pathway in CYN absorption. A minor carrier-mediated transCellular transport has been evidenced. This transport is not affected by low temperatures, suggesting that an active mechanism is not involved. Moreover, the transport through the intestinal monolayer is H+ and GSH dependent and Na+independent. The transport characteristics elucidated in this study prepare the ground for future studies directed at identifying transporters involved in the intestinal absorption of this toxin.
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in vitro study of intestinal transport of fluoride using the caco 2 Cell Line
Food and Chemical Toxicology, 2013Co-Authors: R A Rocha, Vicenta Devesa, Dinoraz VelezAbstract:Water and food are the main sources of fluoride exposure and therefore it is necessary to study intestinal absorption in order to make a correct evaluation of the risk/benefit associated with exposure to fluoride. The present study characterizes intestinal transport of fluoride, using the Caco-2 Cell Line as a model of the intestinal epithelium, and evaluates the coefficients of apparent permeability and intraCellular accumulation in various conditions (pH, temperature, opening of Cell junctions, presence of anions). The results indicate that fluoride is an element with moderate absorption (<70%) in both directions (absorptive and secretory). Both in absorption (apical–basolateral) and in secretion (basolateral–apical) there is transport by the paraCellular pathway, which may be considered predominant. Absorption and secretion of fluoride increase at acid pH levels, possibly because of its non-ionized state at these pHs and/or because of participation of a F−/H+ cotransporter or a F−/OH− antiporter. The results also suggest transCellular participation of mechanisms involved in transport of Cl− and of an active transport in the secretory direction. The present study extend the knowledge on the Cellular transport of fluoride and provide the basis for future studies aimed at identifying potential transporters involved in human fluoride absorption.
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in vitro study of intestinal transport of arsenite monomethylarsonous acid and dimethylarsinous acid by caco 2 Cell Line
Toxicology Letters, 2011Co-Authors: Marta Calatayud, Vicenta Devesa, R Montoro, Dinoraz VelezAbstract:Abstract Arsenic is a pollutant widely distributed in the environment. There are numerous studies on the toxicity of trivalent arsenic forms As(III), MMA(III), and DMA(III), but few data are available on the processes of digestion and absorption of these arsenic species and the mechanisms involved are unknown. The present study evaluated the processes involved in intestinal absorption of trivalent arsenic species, using the Caco-2 Cell model as system. The apparent permeability values obtained for As(III) in apical-basolateral direction (4.6 ± 0.3) × 10 −6 cm/s, showing moderate intestinal absorption. Transport of MMA(III) [ P app = (7.0 ± 0.9) × 10 −6 cm/s] and DMA(III) [ P app = (10.6 ± 1.4) × 10 −6 cm/s] is greater than that of As(III). The Cellular retention of As(III) (0.9–2.4%) was less than that observed for MMA(III) (30%) and DMA(III) (35%). A substantial paraCellular component was observed in transport of As(III) and MMA(III), whereas DMA(III) does not use this pathway for its absorption. For all the trivalent species, transport depends on temperature, with an active transCellular component for MMA(III) and DMA(III). Variations in pH do not affect transport of these species. The presence of GSH and green tea extract significantly alters transport of As(III) across Caco-2 Cells.
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characterization of the intestinal absorption of arsenate monomethylarsonic acid and dimethylarsinic acid using the caco 2 Cell Line
Chemical Research in Toxicology, 2010Co-Authors: Marta Calatayud, Vicenta Devesa, Dinoraz Velez, Jose Gimeno, R MontoroAbstract:Many toxicological studies have been conducted with arsenic species in target organ Cell Lines. However, although epithelial gastrointestinal Cells constitute the first barrier to the absorption of contaminants, studies using intestinal Cells are scarce. The present study examines absorption through the intestinal epithelium of the pentavalent arsenic species most commonly found in foods [arsenate, As(V); monomethylarsonic acid, MMA(V); and dimethylarsinic acid, DMA(V)], using the Caco-2 Cell Line as a model. Different concentrations (1.3−667.6 μM) and culture conditions (media, pH, addition of phosphates, and treatment with ethylenediaminetetraacetic acid) were evaluated to characterize such transport. The apparent permeabilities indicate that the methylated species show low absorption, whereas As(V) is a compound with moderate absorption. The kinetic study shows only a saturable component for MMA(V) transport in the range of concentrations assayed. The existence of paraCellular transport was shown for a...
