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Rguibi Idrissi Hamid - One of the best experts on this subject based on the ideXlab platform.
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The analysis of the canid mitochondrial genome studied in Morocco shows that it is neither wolf (Canis lupus) nor Eurasian jackal (Canis aureus)
'PeerJ', 2016Co-Authors: Urios Vicente, Donat-torres, María P., Ramírez Castillo Carlos, Monroy-vilchis Octavio, Rguibi Idrissi HamidAbstract:The mitochondrial genome of three Moroccan canids has been analysed. Two of them complete and one partial. The sequences are included in GenBank with the accession numbers KT378605 (16721 bp), KT378606 (16734bp) and KT378607 (27809bp) Theses results have been compared with the results currently available in GenBank. In the phylogenetic analysis of the of cytochrome b regions and control region the three are grouped together with Canis lupus lupaster and the Senegalese golden jackal Canis aureus and separate from the wolf Canis lupus and the Eurasian golden jackal Canis aureus. The comparison of the complete mitochondrial genomes with Canis lupus confirms the distance between the two groups. We conclude that they belong to a different species to the wolf Canis lupus and the Eurasian golden jackal. We propose in agreement with (Koepfli et al., 2015) that it´s scientific name be Canis anthus by merit of being the name by which it was classified and published for the first time as a different species by Cuvier in 1824.Urios, V.; Donat-Torres, MP.; Ramírez Castillo, C.; Monroy-Vilchis, O.; Rguibi Idrissi, H. (2016). The analysis of the canid mitochondrial genome studied in Morocco shows that it is neither wolf (Canis lupus) nor Eurasian jackal (Canis aureus). PeerJ Computer Science. 1(1763):1-19. doi:10.7287/peerj.preprints.1763v1Senia1191176
Hamid Rguibi Idrissi - One of the best experts on this subject based on the ideXlab platform.
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the analysis of the canid mitochondrial genome studied in morocco shows that it is neither wolf canis lupus nor eurasian jackal canis aureus
PeerJ, 2016Co-Authors: Vicente Urios, Maria P Donattorres, Carlos Ramirez Castillo, Octavio Monroyvilchis, Hamid Rguibi IdrissiAbstract:The mitochondrial genome of three Moroccan canids has been analysed. Two of them complete and one partial. The sequences are included in GenBank with the accession numbers KT378605 (16721 bp), KT378606 (16734bp) and KT378607 (27809bp) Theses results have been compared with the results currently available in GenBank. In the phylogenetic analysis of the of cytochrome b regions and control region the three are grouped together with Canis lupus lupaster and the Senegalese golden jackal Canis aureus and separate from the wolf Canis lupus and the Eurasian golden jackal Canis aureus. The comparison of the complete mitochondrial genomes with Canis lupus confirms the distance between the two groups. We conclude that they belong to a different species to the wolf Canis lupus and the Eurasian golden jackal. We propose in agreement with (Koepfli et al., 2015) that its scientific name be Canis anthus by merit of being the name by which it was classified and published for the first time as a different species by Cuvier in 1824.
Urios Vicente - One of the best experts on this subject based on the ideXlab platform.
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The analysis of the canid mitochondrial genome studied in Morocco shows that it is neither wolf (Canis lupus) nor Eurasian jackal (Canis aureus)
'PeerJ', 2016Co-Authors: Urios Vicente, Donat-torres, María P., Ramírez Castillo Carlos, Monroy-vilchis Octavio, Rguibi Idrissi HamidAbstract:The mitochondrial genome of three Moroccan canids has been analysed. Two of them complete and one partial. The sequences are included in GenBank with the accession numbers KT378605 (16721 bp), KT378606 (16734bp) and KT378607 (27809bp) Theses results have been compared with the results currently available in GenBank. In the phylogenetic analysis of the of cytochrome b regions and control region the three are grouped together with Canis lupus lupaster and the Senegalese golden jackal Canis aureus and separate from the wolf Canis lupus and the Eurasian golden jackal Canis aureus. The comparison of the complete mitochondrial genomes with Canis lupus confirms the distance between the two groups. We conclude that they belong to a different species to the wolf Canis lupus and the Eurasian golden jackal. We propose in agreement with (Koepfli et al., 2015) that it´s scientific name be Canis anthus by merit of being the name by which it was classified and published for the first time as a different species by Cuvier in 1824.Urios, V.; Donat-Torres, MP.; Ramírez Castillo, C.; Monroy-Vilchis, O.; Rguibi Idrissi, H. (2016). The analysis of the canid mitochondrial genome studied in Morocco shows that it is neither wolf (Canis lupus) nor Eurasian jackal (Canis aureus). PeerJ Computer Science. 1(1763):1-19. doi:10.7287/peerj.preprints.1763v1Senia1191176
Vicente Urios - One of the best experts on this subject based on the ideXlab platform.
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the analysis of the canid mitochondrial genome studied in morocco shows that it is neither wolf canis lupus nor eurasian jackal canis aureus
PeerJ, 2016Co-Authors: Vicente Urios, Maria P Donattorres, Carlos Ramirez Castillo, Octavio Monroyvilchis, Hamid Rguibi IdrissiAbstract:The mitochondrial genome of three Moroccan canids has been analysed. Two of them complete and one partial. The sequences are included in GenBank with the accession numbers KT378605 (16721 bp), KT378606 (16734bp) and KT378607 (27809bp) Theses results have been compared with the results currently available in GenBank. In the phylogenetic analysis of the of cytochrome b regions and control region the three are grouped together with Canis lupus lupaster and the Senegalese golden jackal Canis aureus and separate from the wolf Canis lupus and the Eurasian golden jackal Canis aureus. The comparison of the complete mitochondrial genomes with Canis lupus confirms the distance between the two groups. We conclude that they belong to a different species to the wolf Canis lupus and the Eurasian golden jackal. We propose in agreement with (Koepfli et al., 2015) that its scientific name be Canis anthus by merit of being the name by which it was classified and published for the first time as a different species by Cuvier in 1824.
Onder Duzlu - One of the best experts on this subject based on the ideXlab platform.
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the investigation of some tick borne protozoon and rickettsial infections in dogs by real time pcr and the molecular characterizations of the detected isolates
Ankara Universitesi Veteriner Fakultesi Dergisi, 2014Co-Authors: Onder Duzlu, Abdullah Inci, Alparslan Yildirim, Zuhal Onder, Arif CiloğluAbstract:Bu calisma, Kayseri yoresinde kopeklerden toplanmis 400 kan orneginde Babesia canis vogeli, B. canis canis, B. canis rossi, B. gibsoni, Hepatozoon canis, Ehrlichia canis ve Anaplasma phagocytophilum’un Real Time PCR’la arastirilmasi ve izolatlarin molekuler karakterizasyonlari amaciyla yapilmistir. Real Time PCR sonuclarinda; E. canis, B. canis canis, B. gibsoni, A.phagocytophilum, H. canis ve B. canis vogeli prevalansi sirasiyla %14,5, %12,0, %9,0, %7,8, %5,3 ve %2,3 bulunmus olup B. canis rossi’ye rastlanmamistir. Orneklerin 182’si (%89,7) tek turle, 21’i (%10,3) iki turle enfekte saptanmistir. B.canis canis, B. canis vogeli ve B. gibsoni izolatlarinin 18S rRNA gen bolgesine ait sekanslarinin dunyadaki ayni turden diger izolatlarla ikili kiyaslamalarinda %1,4±0,2, %0,3±0,2 ve %0,9±0,3 genetik farklilik saptanmistir. E. canis icin 16S rRNA gen bolgesi yonunden uc izolatin kendi aralarinda %100 identik oldugu, dunyadaki izolatlarla ise %0,1 farklilik gosterdigi; A. phagocytophilum icin ankA gen bolgesi yonunden uc izolatin kendi aralarinda %99,8±0,2 identik oldugu, dunyadaki izolatlarla ise %0,9±0,3 farklilik gosterdigi; H. canis icin 18S rRNA geni yonunden iki izolatin kendi aralarinda %100 identik oldugu, dunyadaki izolatlarla ise %0,2±0,1 farklilik gosterdikleri tespit edilmistir. Sonuc olarak bu calismayla Kayseri yoresinde kopeklerde kene kaynakli protozoon ve rickettsial enfeksiyonlarin molekuler prevalanslari saptanmis ve enfeksiyonlara yol acan turlerin cesitli gen bolgeleri analiz edilerek molekuler karakterizasyonlari yapilmistir. Tum izolatlarin Genbank kayitlari gerceklestirilmistir
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kopeklerde kene kaynakli bazi protozoon ve rickettsial enfeksiyonlarin real time pcr ile arastirilmasi ve saptanan izolatlarin molekuler karakterizasyonlari
Ankara Universitesi Veteriner Fakultesi Dergisi, 2014Co-Authors: Onder Duzlu, Abdullah Inci, Alparslan Yildirim, Zuhal Onder, Arif CiloğluAbstract:Summary: This study was conducted to investigate Babesia canis vogeli, B. canis canis, B. canis rossi, B. gibsoni, Hepatozoon canis, Ehrlichia canis and Anaplasma phagocytophilum species, and to determine the molecular characterizations of the isolates in totally 400 whole blood samples of dogs in Kayseri region. According to the Real Time PCR results, the prevalence of E.canis, B.canis canis, B. gibsoni, A. phagocytophilum, H. canis, and B. canis vogeli was detected as 14.5%, 12.0%, 9.0%, 7.8%, 5.3%, and 2.3%, respectively while B.canis rossi was not detected in the examined samples. 182 (89.7%) out of the 400 samples were found to be infected with a single parasite species, and 21 (10.3%) were found to be infected with two species. According to the pairwise comparisons of 18S rRNA gene region of the isolates under B. canis canis, B.canis vogeli and B. gibsoni, 1.4±0.2%, 0.3±0.2%, and 0.9±0.3% genetic distance were detected with the other similar isolates from the world, respectively. Based on the 16S rRNA gene sequence alignments, 100% identity was found among the 3 isolates of E. canis while 0.1% genetic difference was determined with the isolates from the world. With respect to ankA gene region of A. phagocytophilum, 99.8±0.2% identity and 0.9±0.3% genetic difference were found among the 3 isolates obtained from Kayseri region and the isolates from the world, respectively. The 2 H. canis isolates were showed 100% identity to each other and 0.2±0.1% genetic difference were determined with the other isolates from the world with respect to 18S rRNA gene region. In conclusion, the molecular prevalence of tick-borne protozoon and rickettsial infections in dogs in Kayseri region was determined and the molecular characterizations of the obtained isolates were performed by analyzing the various gene regions in this study. All isolates were recorded to GenBank.
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kopeklerde kene kaynakli dotless bazi dotless protozoon ve rickettsial enfeksiyonlari dotless n real time pcr ile arasti dotless ri dotless lmasi dotless ve saptanan izolatlari dotless n molekuler karakterizasyonlari dotless
Ankara Universitesi Veteriner Fakultesi Dergisi, 2014Co-Authors: Onder Duzlu, Abdullah Inci, Alparslan Yildirim, Zuhal Onder, Arif CiloǧluAbstract:This study was conducted to investigate Babesia canis vogeli, B. canis canis, B. canis rossi, B. gibsoni, Hepatozoon canis, Ehrlichia canis and Anaplasma phagocytophilum species, and to determine the molecular characterizations of the isolates in totally 400 whole blood samples of dogs in Kayseri region. According to the Real Time PCR results, the prevalence of E.canis, B.canis canis, B. gibsoni, A. phagocytophilum, H. canis, and B. canis vogeli was detected as 14.5%, 12.0%, 9.0%, 7.8%, 5.3%, and 2.3%, respectively while B.canis rossi was not detected in the examined samples. 182 (89.7%) out of the 400 samples were found to be infected with a single parasite species, and 21 (10.3%) were found to be infected with two species. According to the pairwise comparisons of 18S rRNA gene region of the isolates under B. canis canis, B.canis vogeli and B. gibsoni, 1.4±0.2%, 0.3±0.2%, and 0.9±0.3% genetic distance were detected with the other similar isolates from the world, respectively. Based on the 16S rRNA gene sequence alignments, 100% identity was found among the 3 isolates of E. canis while 0.1% genetic difference was determined with the isolates from the world. With respect to ankA gene region of A. phagocytophilum, 99.8±0.2% identity and 0.9±0.3% genetic difference were found among the 3 isolates obtained from Kayseri region and the isolates from the world, respectively. The 2 H. canis isolates were showed 100% identity to each other and 0.2±0.1% genetic difference were determined with the other isolates from the world with respect to 18S rRNA gene region. In conclusion, the molecular prevalence of tick-borne protozoon and rickettsial infections in dogs in Kayseri region was determined and the molecular characterizations of the obtained isolates were performed by analyzing the various gene regions in this study. All isolates were recorded to GenBank. (Less)
