The Experts below are selected from a list of 190605 Experts worldwide ranked by ideXlab platform

Jun Okuda - One of the best experts on this subject based on the ideXlab platform.

Juchan Gang - One of the best experts on this subject based on the ideXlab platform.

  • temporal changes of plasma vitellogenin vtg alkaline labile protein phosphorus alpp Calcium Ca glutamate pyruvate transaminase gpt and hepatosomatic index hsi in the estradiol 17 beta administered immature rockfish sebastes schlegeli
    Journal of fish pathology, 2004
    Co-Authors: Ungi Hwang, Jeongmin Sim, Seungyun Park, Juchan Gang
    Abstract:

    Temporal changes of plasma vitellogenin (VTG), alkaline-labile protein phosphorus (ALPP), Calcium (Ca), glutamate pyruvate transaminase (GPT) and hepatosomatic index (HSI) were examined in the -administered immature rockfish, Sebastes schlegeli. Fish were intraperitoneally injected with (5 ㎎/kg B.W.) in 70% ethanol and then plasma were extracted at 0, 1, 3, 6, 9, 12 and 15 days. VTG band was detected at a molecular weight position of about 170 kDa on Day 3 in SDS-PAGE. This band beCame more distinct at 6 days but its was gradually thinned with time-course, and not detected at 15 days. Plasma ALPP and Ca increased suddenly at 1 day and the highest concentrations were detected at 6 days and then these concentrations decreased gradually with time-course. ALPP and Ca concentrations at 15 days after E2 administration were very similar to that before E2 administration. GPT was increased at 1 day and higher GPT was detected at 3 days. However, GPT was gradually decreased with time-course. GPT and HSI at 15 days after E2 administration were also very similar to that before E2 administration. HSI was also increased at 1 day and the highest value was detected at 3 days and then gradually decreased with time-course. These results suggest that plasma ALPP, Ca, GPT and HSI could be utilized as a biomarker of exogenous E2 exposure in coastal ecosystem, beCause the changes of ALPP, Ca, GPT and HSI after E2 administration are very similar to that of VTG

Seung Min Lee - One of the best experts on this subject based on the ideXlab platform.

  • effects of 4 nonylphenol on the induction of plasma vitellogenin vtg alkaline labile protein phosphorus alpp Calcium Ca glutamate pyruvate transaminase gpt and hepatosomatic index hsi in the immature rockfish sebastes schlegeli
    Environmental Health and Toxicology, 2007
    Co-Authors: Unki Hwang, Hyounkook Jin, Seungyoun Park, Pyoungjoong Kim, Seung Min Lee
    Abstract:

    4-nonylphenol (4-NP)이 해산어류인 조피볼락, Sebastes schlegeli의 혈장 vitellogenin (VTO), alkaline-labile protein phosphorus (ALPP), Calcium (Ca), glutamate pyruvate transaminase (GPT) 및 hepatosomatic index (HSI)에 미치는 영향을 조사하였다. 실험어에 3일간격으로 estradiol-17β (E₂, 5 ㎎/㎏ B.W.) 또는 4-NP (0, 10, 50, 100 및 200 ㎎/㎏ B.W.)을 복강에 2번 주사한 후, 7일째에 채혈과 적출을 통해 혈장과 간장을 수집해 분석이 실시되었다. 대조 실험어에는 용매로 사용된 70% 에탄올만이 투여되었다. E₂ 투여 실험어의 혈장 단백질을 전기 영동상으로 분석한 결과 약 l70kDa의 위치에서 짙은 VTG 밴드가 관찰되었으나, 용매만 투여한 대조 실험어의 혈장에서는 동일 밴드가 관찰되지 않았다. 4-NP 투여한 모든 실험어의 혈장 단백질에서는 E₂ 투여 실험어와 동일한 VTG 밴드가 관찰되었다. 혈장 ALPP와 Ca 농도도 4-NP 투여 실험어에서 E₂ 투여 실험어와 유사하게 증가하였으며, 이들 농도 변화는 VTG 합성과 더불어 증가하는 경향을 나타냈다. 혈장 전위효소인 GPT와 HSI도 E₂ 투여 실험어와 유사하게 4-NP가 투여된 모든 실험어에서 급격히 증가하였다. 이상의 결과로부터 연안생태계 내에서 서식하는 어류가 4-NP과 같은 내분비 장애물질(Endocrine Disrupting Compounds, EDCs)에 의해 영향을 받는지를 규명하기 위한 생물학적 지표로서 VTG와 더불어 혈장 ALPP와 Ca이 사용가능할 것으로 판단된다. 또한, 조피볼락과 같은 해산어가 EDCs에 노출되어 VTG가 합성될 때 간장 기능의 손상으로 혈장 전위효소인 GPT가 일시적으로 증가하고 간장도 비대해져 HSI가 높아지는 것으로 판단된다.

Valeri Leich - One of the best experts on this subject based on the ideXlab platform.

Meghan Murray - One of the best experts on this subject based on the ideXlab platform.