The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
Kanju Ohsawa - One of the best experts on this subject based on the ideXlab platform.
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Contact sex pheromone components of the cowpea weevil, Callosobruchus maculatus.
Journal of chemical ecology, 2007Co-Authors: Satoshi Nojima, Kenji Shimomura, Hiroshi Honda, Izuru Yamamoto, Kanju OhsawaAbstract:The cowpea weevil, Callosobruchus maculatus, is a major pest of stored pulses. Females of this species produce a contact sex pheromone that elicits copulation behavior in males. Pheromone was extracted from filter-paper shelters taken from cages that housed females. Crude ether extract stimulated copulation in male C. maculatus. Initial fractionation showed behavioral activity in acidic and neutral fractions. Furthermore, bioassay-guided fractionation and gas chromatography-mass spectroscopy (GC-MS) analysis of active fractions revealed that the active components of the acidic fraction were 2,6-dimethyloctane-1,8-dioic acid and nonanedioic acid. These components along with the hydrocarbon fraction, a mixture of C(27)-C(35) straight chain and methyl branched hydrocarbons, had a synergistic effect on the behavior of males. Glass dummies treated with an authentic pheromone blend induced copulation behavior in males. The potential roles of the contact sex pheromone of C. maculatus are discussed.
Goro Yabuta - One of the best experts on this subject based on the ideXlab platform.
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Efficient and stereo-selective syntheses of three stereoisomers of the sex pheromone of the cowpea weevil, Callosobruchus maculatus.
Bioscience biotechnology and biochemistry, 2007Co-Authors: Arata Yajima, Masamichi Yamamoto, Tomoo Nukada, Goro YabutaAbstract:An efficient and stereoselective synthesis of three stereoisomers of the sex pheromone of the cowpea weevil, Callosobruchus maculatus, is reported.
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Direct determination of the stereoisomer constitution by 2D-HPLC and stereochemistry–pheromone activity relationship of the copulation release pheromone of the cowpea weevil, Callosobruchus maculatus
Tetrahedron, 2006Co-Authors: Arata Yajima, Tomoo Nukada, Kazuaki Akasaka, Tomonori Nakai, Hiroki Maehara, Hiroshi Ohrui, Goro YabutaAbstract:Abstract The copulation release pheromone of the cowpea weevil, Callosobruchus maculatus, was re-isolated from about 30,000 virgin female. The natural pheromone was confirmed to be stereochemically impure by Ohrui–Akasaka-2D-HPLC methodology. The structure–activity relationship of the pheromone was also clarified.
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Synthesis of the four stereoisomers of 2,6-dimethyloctane-1,8-dioic acid, a component of the copulation release pheromone of the cowpea weevil, Callosobruchus maculatus.
Bioscience biotechnology and biochemistry, 2005Co-Authors: Tomonori Nakai, Arata Yajima, Tomoo Nukada, Kazuaki Akasaka, Hiroshi Ohrui, Takayuki Kaihoku, Miki Ohtaki, Goro YabutaAbstract:A diastereomeric mixture and the four stereoisomers of 2,6-dimethyloctane-1,8-dioic acid (2), a copulation release pheromone of the cowpea weevil, Callosobruchus maculatus, were synthesized. The stereoisomeric purities of the four synthetic isomers of 2 were determined by the HPLC analyses of their bis-2-(2,3-anthracenedicarboximide)-1-cyclohexyl esters.
José Xavier-filho - One of the best experts on this subject based on the ideXlab platform.
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Toxicity of Hydrolyzed Vicilins toward Callosobruchus maculatus and Phytopathogenic Fungi
Journal of agricultural and food chemistry, 2009Co-Authors: Adriana F. Uchoa, Maria Raquel Alcântara De Miranda, Kátia Valevski Sales Fernandes, Antônia E. A. Oliveira, Valdirene Moreira Gomes, Amanda Jardim De Souza, Francisco J. A. Lemos, José Xavier-filhoAbstract:Studies have shown that vicilins (7S storage proteins) from seeds were able to bind to the surface of the Callosobruchus maculatus larval midgut and to the peritrophic matrices of the midguts of Diatraea saccharalis and Tenebrio molitor , inhibiting larval development. Vicilins were also shown to inhibit yeast growth and bind to yeast cells through the association with chitin-containing structures. The present work studies the association of peptides from vicilins of genotypes of Vigna unguiculata (susceptible and resistant to bruchid) with acetylated chitin and the toxicity of vicilin fragments and chitin-binding vicilin fragments to C. maculatus and phytopathogenic fungi. Hydrolysis of vicilins with alpha-chymotrypsin results in a complex mixture of fragments that were separated by chitin-affinity chromatography. Chitin-binding peptides from both genotypes were toxic to C. maculatus larvae, and alpha-chymotrypsin-hydrolyzed vicilins were deleterious to the above insect and to Fusarium oxysporum , Colletotrichum musae , and Saccharomyces cerevisiae fungi.
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Effect of Protein Fractions Isolated from Cereal Grains on the Development and Survival of the Cowpea Weevil, Callosobruchus maculatus
International Journal of Tropical Insect Science, 1995Co-Authors: Lucia B. S. Andrade, V. M. Q. Flores, M. P. Sales, R. A. Morais, A. Blanco-labra, José Xavier-filhoAbstract:—The effect of protein fractions isolated from cereal grains on the development and survival of the bean weevil, Callosobruchus maculatus, was investigated using wheat, rice and maize. These cereals were fractionated into albumins, globulins, prolamins, glutelins and a residue. The fractions were incorporated in artificial seeds which were infested by C. maculatus. Survival (as indicated by LD50) and development (as indicated by WD50) of 20-day-larvae of the bruchid were negatively affected by most of the fractions. Rice globulins were the most detrimental and affected both the survival and development of the larvae, while wheat albumins and globulins had a stronger effect on larval development than on survival. A previously isolated maize α-amylase/trypsin inhibitor showed an LD50 of 1.5% to Callosobruchus maculatus.
Michael Dockery - One of the best experts on this subject based on the ideXlab platform.
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Callosobruchus maculatus — a seed beetle with a future in schools
Journal of Biological Education, 1997Co-Authors: Michael DockeryAbstract:Keeping seed beetles in the classroom or laboratory is relatively easy and the animal offers considerable scope for studying the behaviour of an invertebrate in both primary and secondary schools The seed beetle Callosobruchus maculatus is an organism that is very easy to keep and maintain in classrooms and laboratories, and provides plenty of scope for practicals and projects to support work in the Science National Curriculum from Key Stage 2 (7–11-year-olds] to Key Stage 4 (14–16-year-olds) and beyond. The maintenance of the animals is discussed and descriptions given to allow the identification of the sexes. The use of the seed beetle for studying animal behaviour is exemplified with suggestions for practical and project work. Sources for beetles are given, together with a list of the equipment needed for their study and maintenance.
Antônia E. A. Oliveira - One of the best experts on this subject based on the ideXlab platform.
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Chemical Modifications of Vicilins Interfere with Chitin-Binding Affinity and Toxicity to Callosobruchus maculatus (Coleoptera: Chrysomelidae) Insect: A Combined In Vitro and In Silico Analysis.
Journal of agricultural and food chemistry, 2020Co-Authors: Maria Raquel Alcântara De Miranda, Adriana F. Uchoa, Sarah Rodrigues Ferreira, Kayan Eudorico Ventury, Evenilton Pessoa Costa, Paulo R. L. Do Carmo, Olga Lima Tavares Machado, Kátia Valevski Sales Fernandes, Antônia E. A. OliveiraAbstract:Vicilins are related to cowpea seed resistance towards Callosobruchus maculatus due to their ability to bind to chitinous structures lining larval midgut. However, this binding mechanism is not ful...
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Toxicity of Hydrolyzed Vicilins toward Callosobruchus maculatus and Phytopathogenic Fungi
Journal of agricultural and food chemistry, 2009Co-Authors: Adriana F. Uchoa, Maria Raquel Alcântara De Miranda, Kátia Valevski Sales Fernandes, Antônia E. A. Oliveira, Valdirene Moreira Gomes, Amanda Jardim De Souza, Francisco J. A. Lemos, José Xavier-filhoAbstract:Studies have shown that vicilins (7S storage proteins) from seeds were able to bind to the surface of the Callosobruchus maculatus larval midgut and to the peritrophic matrices of the midguts of Diatraea saccharalis and Tenebrio molitor , inhibiting larval development. Vicilins were also shown to inhibit yeast growth and bind to yeast cells through the association with chitin-containing structures. The present work studies the association of peptides from vicilins of genotypes of Vigna unguiculata (susceptible and resistant to bruchid) with acetylated chitin and the toxicity of vicilin fragments and chitin-binding vicilin fragments to C. maculatus and phytopathogenic fungi. Hydrolysis of vicilins with alpha-chymotrypsin results in a complex mixture of fragments that were separated by chitin-affinity chromatography. Chitin-binding peptides from both genotypes were toxic to C. maculatus larvae, and alpha-chymotrypsin-hydrolyzed vicilins were deleterious to the above insect and to Fusarium oxysporum , Colletotrichum musae , and Saccharomyces cerevisiae fungi.
