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Kanju Ohsawa - One of the best experts on this subject based on the ideXlab platform.
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Aktivitas minyak dan serbuk enam spesies tumbuhan terhadap peneluran dan Mortalitas Callosobruchus sp. (Coleoptera: Bruchidae)
Perhimpunan Entomologi Indonesia, 2017Co-Authors: Dadang Dadang, Udi Saputro, Kanju OhsawaAbstract:Storage pests remain one of the main problems in storage. The pest attacks occur not only in the field, but also in storage. Control should be done to maintain the quality of storedproducts. The environmentally friendly measures should be implemented to avoid negative impacts to the environment and human being. The objective of this research was to study the mortality and oviposition deterrence caused by powder and oil of six plant species against Callosobruchus sp. (Coleoptera: Bruchidae). Flowers of Eugenia aromatica, roots of Vetiveria zizanioides, leaves of Pogostemon cablin, seeds of Ricinus communis, seeds of Foeniculum vulgare, and stems of Cymbopogon citratus were air-dried and milled to yield powder. Oil of six plant species were purchased from local market. Oviposition deterrent bioassay was conducted by no-choice method for both powder and oil, while mortality bioassay was conducted by topical application and residual methods for plant oil only. Several plant species showed high biological activity to Callosobruchus sp. Powder of E. aromatica and oil of V. zizanioides caused high oviposition deterrence, while oil of V. zizanioides and E. aromatica caused high mortality
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variation in mate recognition specificities among four Callosobruchus seed beetles
Entomologia Experimentalis Et Applicata, 2010Co-Authors: Kenji Shimomura, Shunsuke Yajima, Takanori Mimura, Susumu Ishikawa, Kanju OhsawaAbstract:Differentiation of mate recognition systems is one of the important steps for speciation in animals. For some insects, a contact sex pheromone present on the cuticular surface is indispensable in discriminating reproductive partners. In Callosobruchus species (Coleoptera: Chrysomelidae: Bruchinae), contact sex pheromones have been found in two species, Callosobruchus chinensis (L.) and Callosobruchus maculatus (Fabricius). It was suggested, however, that these two species lacked the ability to discriminate their conspecific and/or heterosexual partners. To elucidate this inconsistency, we verified the existence of contact sex pheromones from two other species, Callosobruchus rhodesianus (Pic) and Callosobruchus analis (Fabricius). As a result, unlike C. chinensis and C. maculatus, the males of C. rhodesianus and C. analis were able to discriminate their heterosexual partners. Comparing cross-copulation behavior, i.e., copulation behavior between two species, against these four species indicated that the mate recognition specificities were quite different. Males of C. rhodesianus and C. analis had highly species-specific mating behavior, whereas males of C. chinensis and C. maculatus were much less specific. These results indicate that variation in mate recognition can arise even among congeneric species living in a sympatric environment, and this variation might have arisen during species differentiation. Based on our results in combination with previous reports on interspecific competition, we suggest that the observed asymmetric cross-copulation behavior might be, at least partially, an adaptation for surviving interspecific competition.
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Homofarnesals: Female Sex Attractant Pheromone Components of the Southern Cowpea Weevil, Callosobruchus chinensis
Journal of Chemical Ecology, 2008Co-Authors: Kenji Shimomura, Shunsuke Yajima, Satoshi Nojima, Kanju OhsawaAbstract:The southern cowpea weevil, Callosobruchus chinensis (Coleoptera: Bruchidae), is a major pest of stored legumes in warm temperate and tropical climates. The female sex attractant pheromone was extracted from filter-paper shelters taken from containers that housed virgin females. The extracts were purified by various chromatographic techniques, and the biologically active components in the fractions were screened by gas chromatographic–electroantennographic detection analysis with male antennae. Two compounds that elicited electrophysiological responses were isolated, and gas chromatography–mass spectrometry, nuclear magnetic resonance, and micro-chemical analyses suggested that the active compounds were homofarnesals, (2 Z ,6 E )- and (2 E ,6 E )-7-ethyl-3,11-dimethyl-2,6,10-dodecatrienals. Males of C. chinensis were significantly attracted to filter paper discs loaded with the synthetic compounds at 0.01–0.1 ng compared to solvent control in a Y-tube olfactometer assay. These pheromone components represent unique chemical structures within the genus Callosobruchus .
Larry L. Murdock - One of the best experts on this subject based on the ideXlab platform.
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An Insecticidal N-Acetylglucosamine-Specific Lectin Gene from Griffonia simplicifolia (Leguminosae)
Plant Physiology, 1996Co-Authors: Joseph E. Huesing, Richard E. Shade, Ray A. Bressan, Paul M. Hasegawa, Larry L. MurdockAbstract:Griffonia simplicifolia II, an N-acetylglucosamine-specific legume lectin, has insecticidal activity when fed to the cowpea weevil, Callosobruchus maculatus (F.). A cDNA clone encoding G. simplicifolia II was isolated from a leaf cDNA library, sequenced, and expressed in a bacterial expression system. The recombinant protein exhibited N-acetylglucosamine-binding and insecticidal activity against cowpea weevil, indicating that glycosylation and multimeric structure are not required for these properties. These results support the hypothesis that genes of the legume lectin gene family encode proteins that function in plant defense against herbivores.
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efficacy of ash for controlling infestations of Callosobruchus maculatus f coleoptera bruchidae in stored cowpeas
Journal of Stored Products Research, 1991Co-Authors: Jane L Wolfson, Richard E. Shade, Paul Mentzer, Larry L. MurdockAbstract:Abstract Storing cowpeas, Vigna unguiculata (L.) Walpers, with ash to protect them against Callosobruchus maculatus (F.) is a traditional storage method in northern Cameroon. The amount of ash used and the details of the methodology (i.e. mixing vs layering, the source of ash used) varies among farmers. In experiments designed to simulate traditional ash-storage procedures, it was found that a minimum ratio of 3 parts of ash to 4 parts of cowpeas prevented population growth of C. maculatus and that a 3 cm layer of ash on top of stored seeds prevented infestation by adults.
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rice and stinging nettle lectins insecticidal activity similar to wheat germ agglutinin
Phytochemistry, 1991Co-Authors: Joseph E. Huesing, Larry L. Murdock, Richard E. ShadeAbstract:Previous studies have shown that lectins with specificity for GlcNAc residues, when fed in the diet of the cowpea weevil,Callosobruchus maculatus, cause delays in its development. We have begun anin vivo structure activity analysis to determine what molecular features lead to maximum lectin toxicity. Although rice lectin has a four-fold greater agglutinating activity toward mammalian erythrocytes than wheat germ agglutinin (WGA), its biological activity when fed toC. maculatus is similar to WGA. Stinging nettle lectin, a poor agglutinin, isca two to four times less effective than WGA.
Goro Yabuta - One of the best experts on this subject based on the ideXlab platform.
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Direct Determination of the Stereoisomeric Composition of Callosobruchusic Acid, the Copulation Release Pheromone of the Azuki Bean Weevil, Callosobruchus chinensis L., by the 2D-Ohrui-Akasaka Method
Journal of Chemical Ecology, 2007Co-Authors: Arata Yajima, Kazuaki Akasaka, Masamichi Yamamoto, Sachiko Ohmori, Tomoo Nukada, Goro YabutaAbstract:The stereoisomeric composition of the copulation release pheromone of the azuki bean weevil, Callosobruchus chinensis L., was determined to be R : S = 3.3–3.4:1 by the 2D-Ohrui–Akasaka method.
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direct determination of the stereoisomer constitution by 2d hplc and stereochemistry pheromone activity relationship of the copulation release pheromone of the cowpea weevil Callosobruchus maculatus
Tetrahedron, 2006Co-Authors: Arata Yajima, Kazuaki Akasaka, Tomoo Nukada, Tomonori Nakai, Hiroki Maehara, Hiroshi Ohrui, Goro YabutaAbstract:Abstract The copulation release pheromone of the cowpea weevil, Callosobruchus maculatus, was re-isolated from about 30,000 virgin female. The natural pheromone was confirmed to be stereochemically impure by Ohrui–Akasaka-2D-HPLC methodology. The structure–activity relationship of the pheromone was also clarified.
Richard E. Shade - One of the best experts on this subject based on the ideXlab platform.
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An Insecticidal N-Acetylglucosamine-Specific Lectin Gene from Griffonia simplicifolia (Leguminosae)
Plant Physiology, 1996Co-Authors: Joseph E. Huesing, Richard E. Shade, Ray A. Bressan, Paul M. Hasegawa, Larry L. MurdockAbstract:Griffonia simplicifolia II, an N-acetylglucosamine-specific legume lectin, has insecticidal activity when fed to the cowpea weevil, Callosobruchus maculatus (F.). A cDNA clone encoding G. simplicifolia II was isolated from a leaf cDNA library, sequenced, and expressed in a bacterial expression system. The recombinant protein exhibited N-acetylglucosamine-binding and insecticidal activity against cowpea weevil, indicating that glycosylation and multimeric structure are not required for these properties. These results support the hypothesis that genes of the legume lectin gene family encode proteins that function in plant defense against herbivores.
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a solar heater for disinfesting stored cowpeas on subsistence farms
Journal of Stored Products Research, 1992Co-Authors: L. W. Kitch, Jane L Wolfson, Georges Ntoukam, Richard E. Shade, L L MurdockAbstract:Abstract The construction and testing of a solar heater for eliminating Callosobruchus maculatus (F.) infestations in 50 kg cowpea bulks is described. Research station and on-farm experiments at four different sites in northern Cameroon demonstrated the effectiveness of the solar heater in eliminating C. maculatus infestations. The heaters made of solar radiation-absorbing black plastic covered with a sheet of clear plastic to trap the solar radiation attained temperatures lethal to C. maculatus larvae on four different test dates. On-farm tests coupled with farmer feedback provided evidence that these solar heaters are a practical and useful new approach to preserving cowpeas for use by low resource producers.
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efficacy of ash for controlling infestations of Callosobruchus maculatus f coleoptera bruchidae in stored cowpeas
Journal of Stored Products Research, 1991Co-Authors: Jane L Wolfson, Richard E. Shade, Paul Mentzer, Larry L. MurdockAbstract:Abstract Storing cowpeas, Vigna unguiculata (L.) Walpers, with ash to protect them against Callosobruchus maculatus (F.) is a traditional storage method in northern Cameroon. The amount of ash used and the details of the methodology (i.e. mixing vs layering, the source of ash used) varies among farmers. In experiments designed to simulate traditional ash-storage procedures, it was found that a minimum ratio of 3 parts of ash to 4 parts of cowpeas prevented population growth of C. maculatus and that a 3 cm layer of ash on top of stored seeds prevented infestation by adults.
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rice and stinging nettle lectins insecticidal activity similar to wheat germ agglutinin
Phytochemistry, 1991Co-Authors: Joseph E. Huesing, Larry L. Murdock, Richard E. ShadeAbstract:Previous studies have shown that lectins with specificity for GlcNAc residues, when fed in the diet of the cowpea weevil,Callosobruchus maculatus, cause delays in its development. We have begun anin vivo structure activity analysis to determine what molecular features lead to maximum lectin toxicity. Although rice lectin has a four-fold greater agglutinating activity toward mammalian erythrocytes than wheat germ agglutinin (WGA), its biological activity when fed toC. maculatus is similar to WGA. Stinging nettle lectin, a poor agglutinin, isca two to four times less effective than WGA.
Olajire A. Gbaye - One of the best experts on this subject based on the ideXlab platform.
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Variation in the sensitivity of Callosobruchus (Coleoptera: Bruchidae) acetylcholinesterase to the organophosphate insecticide malaoxon: effect of species, geographical strain and food type
Pest management science, 2012Co-Authors: Olajire A. Gbaye, Graham J. Holloway, Amanda CallaghanAbstract:BACKGROUND: Bruchid beetles, Callosobruchus species, are serious pests of economically important grain legumes; their activity in stores is often controlled by the use of synthetic insecticides. Esterases are known to be involved in insecticide resistance in insects. However, there is a dearth of information on esterase activity in the genus Callosobruchus. In this study, the effect of species, geographical strain and food type on the variation in acetylcholinesterase (AChE) activity and its inhibition by malaoxon (malathion metabolite) was investigated using an in vitro spectrophotometric method. RESULT: AChE activity varied significantly among species and strains and also among legume type used for rearing them. Generally, irrespective of species, strain or food type, the higher the AChE activity of a population, the higher is its inhibition by malaoxon. C. chinensis had the highest AChE activity of the species studied, and in the presence of malaoxon it had the lowest remaining AChE activity, while C. rhodesianus retained the highest activity. CONCLUSION: A first-hand knowledge of AChE activity in regional Callosobruchus in line with the prevailing food types should be of utmost importance to grain legume breeders, researchers on plant materials for bruchid control and pesticide manufacturer/applicators for a robust integrated management of these bruchids. Copyright © 2012 Society of Chemical Industry
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legume type and temperature effects on the toxicity of insecticide to the genus Callosobruchus coleoptera bruchidae
Journal of Stored Products Research, 2011Co-Authors: Olajire A. Gbaye, John Millard, Graham J. HollowayAbstract:Abstract Three bruchid pest species, Callosobruchus maculatus , Callosobruchus chinensis and Callosobruchus rhodesianus , were studied for their response to insecticide toxicity taking into account the separate and interactive effects of temperature and pre-adult food. The food types used were cowpea ( Vigna unguiculata ) and mungbean ( Vigna radiata ). Callosobruchus maculatus was the most tolerant to malathion and the least affected by temperature change while C. rhodesianus was the least tolerant. Over a 4 °C range (23°, 25°, 27 °C), there was generally a significant impact of temperature on the tolerance of the three species to the insecticide. The food type on which the insects developed influenced considerably the degree of insecticide tolerance. Callosobruchus maculatus and C. chinensis populations reared on mungbean had higher tolerance to malathion than their counterparts reared on cowpea, but the opposite was observed in C. rhodesianus populations. The food influence in this study suggested an ancestral cause or fitness cost depending on the species. The interaction of food-by-temperature had no significant effect on malathion toxicity to this genus. Correlation analysis showed C. chinensis to be relatively less sensitive to insecticide concentration over the range studied compared with the other two species.
